Restriction map for
pF1KA0571
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# NO CUT ENZYMES ( 104 enzymes) #
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AatII Acc65I AccII AccIII AclI
AflII AflIII AgeI Aor51HI AscI
AseI AsnI Asp718I AspI AvaI
AviII BamHI BbeI BbrPI BciVI
BclI BcnI BfrI BsaAI BsaBI
BsaWI BseAI BsiWI BsmBI BsmI
BsoBI BspEI BspLU11I BssHII BssSI
Bst1107I BstBI BstUI BstZ17I CpoI
DraI DrdI EagI EclXI Eco47III
Eco52I EcoNI EcoRV EcoT22I FbaI
FseI FspI HaeII HindIII HpaI
KasI KpnI KspI MamI MfeI
MluI MroI MunI MvnI NarI
NciI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I RsrII SacII SalI
SbfI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspI SwaI Tth111I XbaI
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 34 enzymes) #
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AcsI 664 GAATTC [GA]^AATT[CT]
AcyI 774 GACGCC G[GA]^CG[CT]C
AhdI 590 GACACTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1378 GTGCAC G^TGCAC
ApaI 1331 GGGCCC GGGCC^C
ApaLI 1378 GTGCAC G^TGCAC
ApoI 664 GAATTC [GA]^AATT[CT]
Asp700I 272 GAAATGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 590 GACACTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 865 CCTAGG C^CTAGG
BlnI 865 CCTAGG C^CTAGG
BsaHI 774 GACGCC G[GA]^CG[CT]C
BsiEI 1210 CGATCG CG[GA][CT]^CG
BspDI 1492 ATCGAT AT^CGAT
BspHI 545 TCATGA T^CATGA
BstEII 7 GGTGACC G^GT[ACGT]ACC
BstPI 7 GGTGACC G^GT[ACGT]ACC
ClaI 1492 ATCGAT AT^CGAT
Eam1105I 590 GACACTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 7 GGTGACC G^GT[ACGT]ACC
EcoRI 664 GAATTC G^AATTC
Hin1I 774 GACGCC G[GA]^CG[CT]C
MseI 110 TTAA T^TAA
NaeI 650 GCCGGC GCC^GGC
NgoMIV 650 GCCGGC G^CCGGC
NspI 422 ACATGC [GA]CATG^[CT]
PpuMI 806 GGGACCT [GA]G^G[AT]CC[CT]
PvuI 1210 CGATCG CGAT^CG
RcaI 545 TCATGA T^CATGA
ScaI 1571 AGTACT AGT^ACT
SexAI 173 ACCTGGT A^CC[AT]GGT
StuI 99 AGGCCT AGG^CCT
Tru9I 110 TTAA T^TAA
XmnI 272 GAAATGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 38 enzymes) #
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AccI 817 GTAGAC GT^[AC][GT]AC
1309 GTCTAC
AlwNI 1655 CAGTGTCTG CAG[ACGT][ACGT][ACGT]^CTG
1840 CAGGCCCTG
AocI 451 CCTCAGG CC^T[ACGT]AGG
1473 CCTGAGG
AvaII 210 GGTCC G^G[AT]CC
807 GGACC
BanI 526 GGCACC G^G[CT][GA]CC
1903 GGTGCC
BbsI 1231 GAAGAC GAAGAC(2/6)
1833 GAAGAC
BcgI 794 GCAGGGAGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1022 GCAGATCTGTCG
BglI 645 GCCGAGCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
728 GCCTCACAGGC
BglII 227 AGATCT A^GATCT
1024 AGATCT
BlpI 313 GCTGAGC GC^T[ACGT]AGC
318 GCTCAGC
Bpu1102I 313 GCTGAGC GC^T[ACGT]AGC
318 GCTCAGC
BpuAI 1231 GAAGAC GAAGAC(2/6)
1833 GAAGAC
BsaI 999 GAGACC GGTCTC(1/5)
1105 GAGACC
BsgI 792 GTGCAG GTGCAG(16/14)
1139 GTGCAG
Bsp1407I 564 TGTACA T^GTACA
761 TGTACA
BspMI 87 GCAGGT ACCTGC(4/8)
1288 GCAGGT
BsrBI 300 CCGCTC CCGCTC(-3/-3)
3 GAGCGG
BsrDI 584 GCAATG GCAATG(2/0)
1066 GCAATG
BsrGI 564 TGTACA T^GTACA
761 TGTACA
BstAPI 493 GCAGAAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
502 GCAAGGAGTGC
Bsu36I 451 CCTCAGG CC^T[ACGT]AGG
1473 CCTGAGG
CelII 313 GCTGAGC GC^T[ACGT]AGC
318 GCTCAGC
CfoI 351 GCGC GCG^C
446 GCGC
DraIII 786 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
1121 CACAGCGTG
Eco81I 451 CCTCAGG CC^T[ACGT]AGG
1473 CCTGAGG
HhaI 351 GCGC GCG^C
446 GCGC
HinP1I 351 GCGC G^CGC
446 GCGC
HincII 598 GTCAAC GT[CT]^[GA]AC
1423 GTCAAC
HindII 598 GTCAAC GT[CT]^[GA]AC
1423 GTCAAC
MspA1I 311 CAGCTG C[AC]G^C[GT]G
560 CAGCTG
PflMI 1120 CCACAGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1523 CCAAGTCTTGG
PstI 124 CTGCAG CTGCA^G
1846 CTGCAG
PvuII 311 CAGCTG CAG^CTG
560 CAGCTG
SacI 316 GAGCTC GAGCT^C
1749 GAGCTC
SspBI 564 TGTACA T^GTACA
761 TGTACA
TaqI 978 TCGA T^CGA
1493 TCGA
TthHB8I 978 TCGA T^CGA
1493 TCGA
Van91I 1120 CCACAGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1523 CCAAGTCTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 82 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
BalI BanII BbvCI BbvI BfaI
BmyI BpmI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrFI BsrI BssKI BstF5I BstNI
BstXI BstYI Cac8I Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MslI
MspI MvaI MwoI NcoI NdeII
NlaIII NlaIV PleI RsaI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TfiI TseI Tsp45I Tsp509I
TspRI XhoII