Restriction map for
pF1KA0563
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# NO CUT ENZYMES ( 88 enzymes) #
##################################
AatII Acc65I AclI AcyI AflIII
AgeI Alw44I Aor51HI ApaLI Asp718I
AspI AviII AvrII BalI BamHI
BbeI BbrPI BcgI BlnI BsaAI
BsaHI BsiEI BsiWI Bsp1407I BspDI
BspLU11I BsrGI BssSI Bst1107I BstBI
BstXI BstZ17I ClaI CpoI DrdI
EaeI EagI EclXI Eco47III Eco52I
EcoRI EcoRV EcoT22I FseI FspI
HaeII HgaI Hin1I KasI KpnI
KspI MluI MluNI MscI NaeI
NarI NgoMIV NheI NotI NruI
NsiI NspV PacI PflFI PinAI
PmaCI PmeI PmlI Psp1406I PvuI
RsrII SacII SalI SbfI ScaI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SphI Sse8387I SspBI SwaI
Tth111I XmaCI XmaI
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# ONE CUT ENZYMES ( 31 enzymes) #
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AccII 3762 CGCG CG^CG
AscI 3760 GGCGCGCC GG^CGCGCC
Asp700I 3744 GAAACCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbvCI 1516 GCTGAGG CCTCAGC(-5/-2)
BglI 615 GCCTCCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 4808 AGATCT A^GATCT
BsaBI 2941 GATCCATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 4551 CGTCTC CGTCTC(1/5)
BsrFI 3479 ACCGGC [GA]^CCGG[CT]
BssHII 3761 GCGCGC G^CGCGC
BstUI 3762 CGCG CG^CG
Cfr10I 3479 ACCGGC [GA]^CCGG[CT]
DraIII 1220 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
HincII 3388 GTTAAC GT[CT]^[GA]AC
HindII 3388 GTTAAC GT[CT]^[GA]AC
HpaI 3388 GTTAAC GTT^AAC
MamI 2941 GATCCATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1028 CAATTG C^AATTG
MunI 1028 CAATTG C^AATTG
MvnI 3762 CGCG CG^CG
NcoI 61 CCATGG C^CATGG
PaeR7I 1976 CTCGAG C^TCGAG
PflMI 4720 CCAGGATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 131 AGGACCC [GA]G^G[AT]CC[CT]
SacI 2494 GAGCTC GAGCT^C
SapI 4041 GAAGAGC GCTCTTC(1/4)
SpeI 96 ACTAGT A^CTAGT
SspI 548 AATATT AAT^ATT
Van91I 4720 CCAGGATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 1976 CTCGAG C^TCGAG
XmnI 3744 GAAACCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 33 enzymes) #
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AccI 3691 GTCTAC GT^[AC][GT]AC
4001 GTAGAC
AccIII 1446 TCCGGA T^CCGGA
4493 TCCGGA
AflII 3013 CTTAAG C^TTAAG
4083 CTTAAG
AhdI 694 GACATCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1143 GACCCCAGGTC
AseI 1456 ATTAAT AT^TAAT
4654 ATTAAT
AsnI 1456 ATTAAT AT^TAAT
4654 ATTAAT
AspEI 694 GACATCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1143 GACCCCAGGTC
AspHI 2494 GAGCTC G[AT]GC[AT]^C
4662 GAGCAC
AvaI 1976 CTCGAG C^[CT]CG[GA]G
4366 CCCGAG
BanI 2593 GGCACC G^G[CT][GA]CC
3574 GGTGCC
BciVI 4623 GGATAC GTATCC(6/5)
570 GTATCC
BclI 252 TGATCA T^GATCA
4410 TGATCA
BfrI 3013 CTTAAG C^TTAAG
4083 CTTAAG
BsaWI 1446 TCCGGA [AT]^CCGG[AT]
4493 TCCGGA
BseAI 1446 TCCGGA T^CCGGA
4493 TCCGGA
BsiHKAI 2494 GAGCTC G[AT]GC[AT]^C
4662 GAGCAC
BsoBI 1976 CTCGAG C^[CT]CG[GA]G
4366 CCCGAG
BspEI 1446 TCCGGA T^CCGGA
4493 TCCGGA
BsrBI 8 CCGCTC CCGCTC(-3/-3)
136 CCGCTC
BstAPI 2880 GCACGGAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4177 GCAAGAAATGC
BstEII 915 GGTTACC G^GT[ACGT]ACC
1192 GGTCACC
BstPI 915 GGTTACC G^GT[ACGT]ACC
1192 GGTCACC
Eam1105I 694 GACATCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1143 GACCCCAGGTC
EcoO65I 915 GGTTACC G^GT[ACGT]ACC
1192 GGTCACC
FbaI 252 TGATCA T^GATCA
4410 TGATCA
MaeII 1247 ACGT A^CGT
4346 ACGT
MroI 1446 TCCGGA T^CCGGA
4493 TCCGGA
NdeI 2487 CATATG CA^TATG
3067 CATATG
NspI 3145 GCATGT [GA]CATG^[CT]
4220 ACATGC
PshBI 1456 ATTAAT AT^TAAT
4654 ATTAAT
SexAI 1776 ACCAGGT A^CC[AT]GGT
1946 ACCAGGT
XbaI 657 TCTAGA T^CTAGA
2976 TCTAGA
XcmI 232 CCAGCAGACCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
460 CCAGCTCAGCGTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 106 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI AocI ApaI ApoI AvaII
BanII BbsI BbvI BcnI BfaI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaI BsaJI BseRI BsgI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
BspHI BspMI BsrDI BsrI BssKI
BstF5I BstNI BstYI Bsu36I Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraI DraII DsaI EarI Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PleI PshAI
PstI PvuII RcaI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII