Restriction map for
pF1KA0512
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# NO CUT ENZYMES ( 113 enzymes) #
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AatII AccIII AclI AcyI AflII
AflIII AgeI Alw44I ApaLI AscI
AspHI AspI AviII AvrII BamHI
BbeI BbrPI BcgI BciVI BfrI
BglII BlnI BlpI Bpu1102I BsaBI
BsaHI BsaWI BseAI BseRI BsgI
BsiEI BsiHKAI BsiWI BsmBI BsmI
BspDI BspEI BspHI BspLU11I BssHII
BssSI Bst1107I BstXI BstYI BstZ17I
CelII ClaI CpoI DraI DraIII
DrdI DsaI EagI EclXI Eco52I
EcoT22I FseI FspI HgaI Hin1I
HincII HindII HindIII HpaI KasI
KspI MamI MfeI MflI MluI
MroI MunI NarI NcoI NdeI
NheI NotI NruI NsiI NspI
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SfiI
SfoI SgrAI SmlI SnaBI SphI
Sse8387I SspI SwaI Tth111I Van91I
XbaI XhoI XhoII
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# ONE CUT ENZYMES ( 44 enzymes) #
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Acc65I 831 GGTACC G^GTACC
AocI 373 CCTAAGG CC^T[ACGT]AGG
Aor51HI 310 AGCGCT AGC^GCT
ApaI 245 GGGCCC GGGCC^C
AseI 1702 ATTAAT AT^TAAT
AsnI 1702 ATTAAT AT^TAAT
Asp700I 1887 GAACAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 831 GGTACC G^GTACC
BbsI 1094 GAAGAC GAAGAC(2/6)
BclI 1307 TGATCA T^GATCA
BpuAI 1094 GAAGAC GAAGAC(2/6)
BsaAI 1681 TACGTG [CT]AC^GT[GA]
Bsp1407I 1400 TGTACA T^GTACA
BspMI 1511 ACCTGC ACCTGC(4/8)
BsrBI 827 GAGCGG CCGCTC(-3/-3)
BsrFI 1388 GCCGGC [GA]^CCGG[CT]
BsrGI 1400 TGTACA T^GTACA
BstBI 1596 TTCGAA TT^CGAA
BstEII 576 GGTGACC G^GT[ACGT]ACC
BstPI 576 GGTGACC G^GT[ACGT]ACC
Bsu36I 373 CCTAAGG CC^T[ACGT]AGG
Cfr10I 1388 GCCGGC [GA]^CCGG[CT]
EarI 105 GAAGAG CTCTTC(1/4)
Eco47III 310 AGCGCT AGC^GCT
Eco81I 373 CCTAAGG CC^T[ACGT]AGG
EcoNI 993 CCTGGCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 576 GGTGACC G^GT[ACGT]ACC
EcoRI 1768 GAATTC G^AATTC
EcoRV 1423 GATATC GAT^ATC
FbaI 1307 TGATCA T^GATCA
KpnI 831 GGTACC GGTAC^C
Ksp632I 105 GAAGAG CTCTTC(1/4)
MaeII 1682 ACGT A^CGT
MslI 570 CACTGAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 1388 GCCGGC GCC^GGC
NgoMIV 1388 GCCGGC G^CCGGC
NspV 1596 TTCGAA TT^CGAA
PshBI 1702 ATTAAT AT^TAAT
SexAI 859 ACCAGGT A^CC[AT]GGT
SfuI 1596 TTCGAA TT^CGAA
SpeI 1881 ACTAGT A^CTAGT
SspBI 1400 TGTACA T^GTACA
StuI 1284 AGGCCT AGG^CCT
XmnI 1887 GAACAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AccI 70 GTCTAC GT^[AC][GT]AC
910 GTAGAC
AccII 7 CGCG CG^CG
1156 CGCG
AlwI 1198 GATCC GGATC(4/5)
1321 GATCC
BalI 113 TGGCCA TGG^CCA
1349 TGGCCA
BsaI 92 GAGACC GGTCTC(1/5)
1069 GAGACC
BstUI 7 CGCG CG^CG
1156 CGCG
EaeI 113 TGGCCA [CT]^GGCC[GA]
1349 TGGCCA
FauI 667 CCCGC CCCGC(4/6)
34 GCGGG
HaeII 310 AGCGCT [GA]GCGC^[CT]
1115 AGCGCC
MluNI 113 TGGCCA TGG^CCA
1349 TGGCCA
MscI 113 TGGCCA TGG^CCA
1349 TGGCCA
MvnI 7 CGCG CG^CG
1156 CGCG
SmaI 427 CCCGGG CCC^GGG
625 CCCGGG
TaqI 188 TCGA T^CGA
1597 TCGA
Tsp45I 433 GTGAC ^GT[GC]AC
577 GTGAC
TthHB8I 188 TCGA T^CGA
1597 TCGA
XcmI 101 CCAAGAAGAGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
345 CCAAGAGGCAGATGG
XmaCI 427 CCCGGG C^CCGGG
625 CCCGGG
XmaI 427 CCCGGG C^CCGGG
625 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 82 enzymes) #
#########################################
AciI AcsI AfaI AhdI AluI
AlwNI ApoI AspEI AvaI AvaII
BanI BanII BbvCI BbvI BcnI
BfaI BglI BmyI BpmI BsaJI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrDI BsrI BssKI BstAPI
BstF5I BstNI Cac8I CfoI Cfr13I
DdeI DpnII DraII Eam1105I EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI MaeI MaeIII MboI
MboII MnlI MseI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TfiI Tru9I TseI
Tsp509I TspRI