Restriction map for
pF1KA0474
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# NO CUT ENZYMES ( 96 enzymes) #
##################################
Acc65I AccI AclI AflII AgeI
AhdI AlwNI Aor51HI AscI AseI
AsnI Asp700I Asp718I AspEI AviII
AvrII BamHI BbeI BcgI BciVI
BfrI BglII BlnI BsiEI BsiWI
BsmI Bsp1407I BspDI BspHI BspLU11I
BsrGI BssHII Bst1107I BstBI BstEII
BstPI BstZ17I ClaI CpoI DraI
DraIII EagI Eam1105I EclXI Eco47III
Eco52I EcoO65I EcoRI EcoRV EcoT22I
FseI FspI HincII HindII HpaI
KasI KpnI KspI MfeI MluI
MslI MunI NarI NdeI NheI
NotI NruI NsiI NspV PacI
PinAI PmeI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SbfI ScaI SexAI SfoI SfuI
SgrAI SmaI SnaBI Sse8387I SspBI
SspI SwaI XbaI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AatII 487 GACGTC GACGT^C
AccIII 1304 TCCGGA T^CCGGA
AflIII 800 ACGTGT A^C[GA][CT]GT
Alw44I 97 GTGCAC G^TGCAC
AocI 1868 CCTCAGG CC^T[ACGT]AGG
ApaLI 97 GTGCAC G^TGCAC
AspI 1921 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 172 GGCACC G^G[CT][GA]CC
BbvCI 964 GCTGAGG CCTCAGC(-5/-2)
BclI 1106 TGATCA T^GATCA
BfaI 1937 CTAG C^TAG
BlpI 1338 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1338 GCTGAGC GC^T[ACGT]AGC
BsaBI 1992 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 1304 TCCGGA T^CCGGA
BspEI 1304 TCCGGA T^CCGGA
BspMI 377 ACCTGC ACCTGC(4/8)
BsrDI 1430 CATTGC GCAATG(2/0)
BsrFI 1960 GCCGGC [GA]^CCGG[CT]
BstAPI 2019 GCAGCACATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 1805 CCAGCGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1997 AGATCC [GA]^GATC[CT]
Bsu36I 1868 CCTCAGG CC^T[ACGT]AGG
CelII 1338 GCTGAGC GC^T[ACGT]AGC
Cfr10I 1960 GCCGGC [GA]^CCGG[CT]
DrdI 553 GACGAGCATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1868 CCTCAGG CC^T[ACGT]AGG
FbaI 1106 TGATCA T^GATCA
HindIII 601 AAGCTT A^AGCTT
MaeI 1937 CTAG C^TAG
MamI 1992 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 1997 AGATCC [GA]^GATC[CT]
MroI 1304 TCCGGA T^CCGGA
MseI 707 TTAA T^TAA
NaeI 1960 GCCGGC GCC^GGC
NgoMIV 1960 GCCGGC G^CCGGC
PaeR7I 241 CTCGAG C^TCGAG
PflFI 1921 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1317 CCAGTCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacI 1698 GAGCTC GAGCT^C
SapI 1524 GAAGAGC GCTCTTC(1/4)
SfiI 976 GGCCCTGATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SpeI 1936 ACTAGT A^CTAGT
SphI 942 GCATGC GCATG^C
Tru9I 707 TTAA T^TAA
Tth111I 1921 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1317 CCAGTCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 241 CTCGAG C^TCGAG
XhoII 1997 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AcsI 673 GAATTT [GA]^AATT[CT]
1087 GAATTT
AfaI 351 GTAC GT^AC
765 GTAC
AlwI 1998 GATCC GGATC(4/5)
498 GGATC
ApaI 975 GGGCCC GGGCC^C
1548 GGGCCC
ApoI 673 GAATTT [GA]^AATT[CT]
1087 GAATTT
BalI 681 TGGCCA TGG^CCA
1409 TGGCCA
BbrPI 799 CACGTG CAC^GTG
1908 CACGTG
BsaAI 799 CACGTG [CT]AC^GT[GA]
1908 CACGTG
BsaI 1735 GAGACC GGTCTC(1/5)
999 GGTCTC
BsaWI 269 ACCGGA [AT]^CCGG[AT]
1304 TCCGGA
BsgI 958 GTGCAG GTGCAG(16/14)
1603 GTGCAG
BssSI 100 CACGAG CACGAG(-5/-1)
1533 CACGAG
EcoNI 990 CCTCTACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1407 CCTGGCCAAGG
HaeII 1573 AGCGCC [GA]GCGC^[CT]
1758 GGCGCT
MluNI 681 TGGCCA TGG^CCA
1409 TGGCCA
MscI 681 TGGCCA TGG^CCA
1409 TGGCCA
NcoI 1322 CCATGG C^CATGG
1331 CCATGG
PmaCI 799 CACGTG CAC^GTG
1908 CACGTG
PmlI 799 CACGTG CAC^GTG
1908 CACGTG
PpuMI 120 AGGACCC [GA]G^G[AT]CC[CT]
1973 GGGACCC
PstI 214 CTGCAG CTGCA^G
697 CTGCAG
PvuII 2002 CAGCTG CAG^CTG
2032 CAGCTG
RsaI 351 GTAC GT^AC
765 GTAC
StuI 723 AGGCCT AGG^CCT
1848 AGGCCT
TaqI 242 TCGA T^CGA
1586 TCGA
TfiI 757 GAATC G^A[AT]TC
1460 GATTC
TthHB8I 242 TCGA T^CGA
1586 TCGA
XcmI 1220 CCATGATGGGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1322 CCATGGATGCCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 85 enzymes) #
#########################################
AccII AciI AcyI AluI AspHI
AvaI AvaII BanII BbsI BbvI
BcnI BglI BmyI BpmI BpuAI
BsaHI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I BsrBI BsrI BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeII MaeIII
MboI MboII MnlI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PleI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TseI Tsp45I Tsp509I TspRI