Restriction map for
pF1KA0455
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# NO CUT ENZYMES ( 102 enzymes) #
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AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI Aor51HI ApaI
AscI Asp718I AviII AvrII BalI
BamHI BbrPI BciVI BfrI BglII
BlnI BsaAI BsaBI BseAI BsiEI
BsiWI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrFI BsrGI BssSI Bst1107I
BstBI BstZ17I Cfr10I ClaI CpoI
DraI DrdI EagI EarI EclXI
Eco47III Eco52I EcoNI EcoT22I FseI
FspI HincII HindII HpaI KpnI
Ksp632I MaeII MamI MfeI MluI
MluNI MroI MscI MunI NaeI
NdeI NgoMIV NotI NruI NsiI
NspV PacI PaeR7I PflMI PinAI
PmaCI PmeI PmlI Psp1406I PstI
PvuI RcaI RsrII SalI SapI
SbfI ScaI SexAI SfiI SfuI
SgrAI SmlI SnaBI SpeI Sse8387I
SspBI SspI StuI SwaI Van91I
XbaI XhoI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AhdI 518 GACGAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 701 CAGGATCTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 481 ATTAAT AT^TAAT
AsnI 481 ATTAAT AT^TAAT
Asp700I 421 GAAGGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 518 GACGAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanI 49 GGCGCC G^G[CT][GA]CC
BbeI 49 GGCGCC GGCGC^C
BbvCI 84 CCTCAGC CCTCAGC(-5/-2)
BcgI 139 GCAGGGATGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 176 TGATCA T^GATCA
BglI 1548 GCCTGGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 375 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 375 GCTTAGC GC^T[ACGT]AGC
BsaWI 314 ACCGGA [AT]^CCGG[AT]
BsgI 1806 CTGCAC GTGCAG(16/14)
BsmI 1301 GCATTC GAATGC(1/-1)
BspMI 396 ACCTGC ACCTGC(4/8)
BsrBI 1322 CCGCTC CCGCTC(-3/-3)
BsrDI 2075 GCAATG GCAATG(2/0)
BssHII 6 GCGCGC G^CGCGC
BstAPI 757 GCAACCCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1230 GGTTACC G^GT[ACGT]ACC
BstPI 1230 GGTTACC G^GT[ACGT]ACC
BstXI 1482 CCATGGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 375 GCTTAGC GC^T[ACGT]AGC
DraII 1585 GGGTCCT [GA]G^G[ACGT]CC[CT]
DraIII 1344 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 24 TGGCCG [CT]^GGCC[GA]
Eam1105I 518 GACGAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 1585 GGGTCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 1230 GGTTACC G^GT[ACGT]ACC
EcoRI 425 GAATTC G^AATTC
FbaI 176 TGATCA T^GATCA
HindIII 826 AAGCTT A^AGCTT
KasI 49 GGCGCC G^GCGCC
KspI 27 CCGCGG CCGC^GG
NarI 49 GGCGCC GG^CGCC
NheI 1147 GCTAGC G^CTAGC
PpuMI 1585 GGGTCCT [GA]G^G[AT]CC[CT]
PshAI 313 GACCGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 481 ATTAAT AT^TAAT
SacI 1984 GAGCTC GAGCT^C
SacII 27 CCGCGG CCGC^GG
SfoI 49 GGCGCC GGC^GCC
XmnI 421 GAAGGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 17 enzymes) #
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AcyI 49 GGCGCC G[GA]^CG[CT]C
1024 GACGCC
AocI 1029 CCTCAGG CC^T[ACGT]AGG
1609 CCTGAGG
BsaHI 49 GGCGCC G[GA]^CG[CT]C
1024 GACGCC
BsmBI 1022 GAGACG CGTCTC(1/5)
2146 GAGACG
Bsu36I 1029 CCTCAGG CC^T[ACGT]AGG
1609 CCTGAGG
Eco81I 1029 CCTCAGG CC^T[ACGT]AGG
1609 CCTGAGG
EcoRV 580 GATATC GAT^ATC
602 GATATC
EcoT14I 1211 CCATGG C^C[AT][AT]GG
1482 CCATGG
Hin1I 49 GGCGCC G[GA]^CG[CT]C
1024 GACGCC
NcoI 1211 CCATGG C^CATGG
1482 CCATGG
SfcI 563 CTACAG C^T[GA][CT]AG
1271 CTGTAG
SmaI 109 CCCGGG CCC^GGG
127 CCCGGG
SphI 326 GCATGC GCATG^C
1544 GCATGC
StyI 1211 CCATGG C^C[AT][AT]GG
1482 CCATGG
XcmI 1479 CCACCATGGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1842 CCATGAGCCCAGTGG
XmaCI 109 CCCGGG C^CCGGG
127 CCCGGG
XmaI 109 CCCGGG C^CCGGG
127 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 93 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
Alw44I AlwI ApaLI ApoI AspHI
AspI AvaI AvaII BanII BbsI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DsaI EcoRII FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI MaeI MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PflFI PleI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Tth111I TthHB8I XhoII