Restriction map for
pF1KA0439
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# NO CUT ENZYMES ( 88 enzymes) #
##################################
AatII Acc65I AccI AflII AgeI
Alw44I Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AviII AvrII
BbeI BbrPI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsgI BsmBI BspDI BspHI BspLU11I
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I CelII ClaI
CpoI DraII Eco47III EcoO109I EcoO65I
EcoT22I FspI HindIII HpaI KasI
KpnI KspI MamI MfeI MluI
MunI NarI NcoI NheI NotI
NsiI NspV PacI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
PvuI RcaI RsrII SacII SalI
SapI SbfI SexAI SfiI SfoI
SfuI SnaBI SpeI SphI Sse8387I
SwaI XbaI XcmI
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# ONE CUT ENZYMES ( 68 enzymes) #
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AccII 2294 CGCG CG^CG
AccIII 853 TCCGGA T^CCGGA
AclI 1266 AACGTT AA^CGTT
AcyI 2248 GACGCC G[GA]^CG[CT]C
AflIII 320 ACGTGT A^C[GA][CT]GT
AhdI 1496 GACCAGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 550 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 547 CCTCAGG CC^T[ACGT]AGG
AspEI 1496 GACCAGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 2390 GAGCTC G[AT]GC[AT]^C
AspI 2002 GACTTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 1565 TGGCCA TGG^CCA
BamHI 1862 GGATCC G^GATCC
BanI 955 GGTGCC G^G[CT][GA]CC
BcgI 669 CGACGCAGTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2261 GTATCC GTATCC(6/5)
BfaI 1514 CTAG C^TAG
BsaHI 2248 GACGCC G[GA]^CG[CT]C
BsaI 457 GAGACC GGTCTC(1/5)
BseAI 853 TCCGGA T^CCGGA
BsiEI 1107 CGGCCG CG[GA][CT]^CG
BsiHKAI 2390 GAGCTC G[AT]GC[AT]^C
BsiWI 972 CGTACG C^GTACG
BsmI 2172 GCATTC GAATGC(1/-1)
Bsp1407I 717 TGTACA T^GTACA
BspEI 853 TCCGGA T^CCGGA
BspMI 1407 ACCTGC ACCTGC(4/8)
BsrBI 384 CCGCTC CCGCTC(-3/-3)
BsrDI 2361 GCAATG GCAATG(2/0)
BsrGI 717 TGTACA T^GTACA
BstUI 2294 CGCG CG^CG
BstXI 1075 CCACCCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 547 CCTCAGG CC^T[ACGT]AGG
DraI 1200 TTTAAA TTT^AAA
DraIII 291 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 38 GACCAAGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1107 CGGCCG C^GGCCG
Eam1105I 1496 GACCAGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 1107 CGGCCG C^GGCCG
Eco52I 1107 CGGCCG C^GGCCG
Eco81I 547 CCTCAGG CC^T[ACGT]AGG
EcoRI 1247 GAATTC G^AATTC
EcoRV 1414 GATATC GAT^ATC
FseI 1108 GGCCGGCC GGCCGG^CC
Hin1I 2248 GACGCC G[GA]^CG[CT]C
MaeI 1514 CTAG C^TAG
MluNI 1565 TGGCCA TGG^CCA
MroI 853 TCCGGA T^CCGGA
MscI 1565 TGGCCA TGG^CCA
MvnI 2294 CGCG CG^CG
NruI 2293 TCGCGA TCG^CGA
NspI 2398 ACATGC [GA]CATG^[CT]
PaeR7I 56 CTCGAG C^TCGAG
PflFI 2002 GACTTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Psp1406I 1266 AACGTT AA^CGTT
PvuII 2344 CAGCTG CAG^CTG
SacI 2390 GAGCTC GAGCT^C
ScaI 1628 AGTACT AGT^ACT
SfaNI 763 GATGC GCATC(5/9)
SgrAI 373 CACCGGCG C[GA]^CCGG[CT]G
SmaI 226 CCCGGG CCC^GGG
SspBI 717 TGTACA T^GTACA
SspI 1841 AATATT AAT^ATT
StuI 1674 AGGCCT AGG^CCT
Tth111I 2002 GACTTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 56 CTCGAG C^TCGAG
XmaCI 226 CCCGGG C^CCGGG
XmaI 226 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 21 enzymes) #
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BbsI 406 GAAGAC GAAGAC(2/6)
1306 GAAGAC
BbvCI 915 CCTCAGC CCTCAGC(-5/-2)
2341 CCTCAGC
BbvI 367 GCAGC GCAGC(8/12)
831 GCAGC
BclI 1125 TGATCA T^GATCA
1278 TGATCA
BpuAI 406 GAAGAC GAAGAC(2/6)
1306 GAAGAC
DsaI 1637 CCACGG C^C[GA][CT]GG
2468 CCGTGG
EarI 1462 GAAGAG CTCTTC(1/4)
1718 GAAGAG
EcoNI 638 CCTCCTCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1666 CCTAATTCAGG
FbaI 1125 TGATCA T^GATCA
1278 TGATCA
HaeII 377 GGCGCT [GA]GCGC^[CT]
1098 AGCGCC
HincII 166 GTTGAC GT[CT]^[GA]AC
271 GTCAAC
HindII 166 GTTGAC GT[CT]^[GA]AC
271 GTCAAC
Ksp632I 1462 GAAGAG CTCTTC(1/4)
1718 GAAGAG
MslI 1252 CACTTGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2463 CATGGCCGTG
MspA1I 946 CCGCTG C[AC]G^C[GT]G
2344 CAGCTG
NaeI 902 GCCGGC GCC^GGC
1109 GCCGGC
NdeI 1180 CATATG CA^TATG
2426 CATATG
NgoMIV 902 GCCGGC G^CCGGC
1109 GCCGGC
PstI 1318 CTGCAG CTGCA^G
2272 CTGCAG
SfcI 1318 CTGCAG C^T[GA][CT]AG
2272 CTGCAG
TseI 367 GCAGC G^C[AT]GC
831 GCAGC
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# 3 OR MORE CUT ENZYMES ( 81 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI Asp700I AvaI AvaII BanII
BcnI BmyI BpmI BsaJI BsaWI
BseRI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrFI BsrI BssKI
BstF5I BstNI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII EaeI
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI MaeII
MaeIII MboI MboII MflI MnlI
MseI MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PflMI PleI
RsaI Sau3AI Sau96I ScrFI SmlI
StyI TaqI TfiI Tru9I Tsp45I
Tsp509I TspRI TthHB8I Van91I XhoII
XmnI