Restriction map for
pF1KA0412
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# NO CUT ENZYMES ( 137 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI AlwNI AocI Aor51HI AscI
AseI AsnI Asp718I AspEI AspI
AviII AvrII BamHI BbeI BbrPI
BbvCI BcgI BciVI BfrI BglI
BglII BlnI BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BsiEI BsiWI
BsmBI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrFI BsrGI BssHII
Bst1107I BstBI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I CfoI Cfr10I
ClaI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRI EcoRV FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HincII
HindII HindIII HpaI KasI KpnI
KspI MaeII MamI MfeI MluI
MroI MunI MvnI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NspV PacI PflFI PflMI PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PstI PvuI RcaI SacI SacII
SalI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SwaI Tth111I Van91I XbaI
XmaCI XmaI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AcsI 668 AAATTC [GA]^AATT[CT]
AfaI 114 GTAC GT^AC
Alw44I 948 GTGCAC G^TGCAC
ApaLI 948 GTGCAC G^TGCAC
ApoI 668 AAATTC [GA]^AATT[CT]
AvaI 1204 CTCGAG C^[CT]CG[GA]G
BalI 960 TGGCCA TGG^CCA
BclI 1839 TGATCA T^GATCA
BseRI 76 GAGGAG GAGGAG(10/8)
BsoBI 1204 CTCGAG C^[CT]CG[GA]G
BsrBI 894 CCGCTC CCGCTC(-3/-3)
BsrDI 866 GCAATG GCAATG(2/0)
BssSI 1494 CACGAG CACGAG(-5/-1)
BstAPI 866 GCAATGAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CpoI 20 CGGACCG CG^G[AT]CCG
DraI 607 TTTAAA TTT^AAA
DsaI 214 CCATGG C^C[GA][CT]GG
EaeI 960 TGGCCA [CT]^GGCC[GA]
EarI 592 GAAGAG CTCTTC(1/4)
EcoNI 111 CCTGTACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 1678 ATGCAT ATGCA^T
FauI 1478 CCCGC CCCGC(4/6)
FbaI 1839 TGATCA T^GATCA
Ksp632I 592 GAAGAG CTCTTC(1/4)
MluNI 960 TGGCCA TGG^CCA
MscI 960 TGGCCA TGG^CCA
NcoI 214 CCATGG C^CATGG
NsiI 1678 ATGCAT ATGCA^T
PaeR7I 1204 CTCGAG C^TCGAG
PpuMI 1403 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 244 GACACCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsaI 114 GTAC GT^AC
RsrII 20 CGGACCG CG^G[AT]CCG
SspI 1804 AATATT AAT^ATT
XcmI 192 CCACCTAGAGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1204 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 20 enzymes) #
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ApaI 26 GGGCCC GGGCC^C
1340 GGGCCC
BanI 1079 GGCACC G^G[CT][GA]CC
1247 GGCACC
BcnI 24 CCGGG CC^[GC]GG
1097 CCGGG
BfaI 97 CTAG C^TAG
196 CTAG
BlpI 100 GCTCAGC GC^T[ACGT]AGC
178 GCTGAGC
Bpu1102I 100 GCTCAGC GC^T[ACGT]AGC
178 GCTGAGC
BsmI 694 GAATGC GAATGC(1/-1)
871 GAATGC
BstYI 432 AGATCC [GA]^GATC[CT]
576 AGATCC
Cac8I 300 GCCAGC GC[ACGT]^[ACGT]GC
372 GCAAGC
CelII 100 GCTCAGC GC^T[ACGT]AGC
178 GCTGAGC
EcoT14I 71 CCAAGG C^C[AT][AT]GG
214 CCATGG
MaeI 97 CTAG C^TAG
196 CTAG
MflI 432 AGATCC [GA]^GATC[CT]
576 AGATCC
NciI 24 CCGGG CC^[GC]GG
1097 CCGGG
NspI 1481 GCATGT [GA]CATG^[CT]
1584 ACATGC
PvuII 88 CAGCTG CAG^CTG
488 CAGCTG
SmlI 1204 CTCGAG C^T[CT][GA]AG
1240 CTCAAG
StyI 71 CCAAGG C^C[AT][AT]GG
214 CCATGG
Tsp509I 669 AATT ^AATT
901 AATT
XhoII 432 AGATCC [GA]^GATC[CT]
576 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AciI AluI AlwI Asp700I AspHI
AvaII BanII BbsI BbvI BmyI
BpmI BpuAI BsaJI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI Cfr13I
DdeI DpnII DraII EcoO109I EcoRII
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI MaeIII MboI
MboII MnlI MseI MslI MspA1I
MspI MvaI MwoI NdeII NlaIII
NlaIV PleI Sau3AI Sau96I ScrFI
SfaNI StuI TaqI TfiI Tru9I
TseI Tsp45I TspRI TthHB8I XmnI