Restriction map for
pF1KA0411
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# NO CUT ENZYMES ( 66 enzymes) #
##################################
AatII Acc65I AclI AflII AscI
AseI AsnI Asp718I AviII BbrPI
BciVI BclI BfrI BglII BsaAI
BsaBI BsiWI BsmBI BsrBI BssHII
Bst1107I BstBI BstZ17I CpoI DraIII
EcoNI EcoRI FbaI FseI FspI
HindIII HpaI KpnI KspI MamI
MfeI MluI MunI NcoI NdeI
NheI NotI NspV PacI PflMI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I RsrII SacII SapI SbfI
SexAI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SwaI Van91I XbaI
XcmI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AccI 2742 GTCGAC GT^[AC][GT]AC
AccIII 842 TCCGGA T^CCGGA
AgeI 3125 ACCGGT A^CCGGT
Alw44I 1765 GTGCAC G^TGCAC
AocI 1705 CCTAAGG CC^T[ACGT]AGG
ApaI 2565 GGGCCC GGGCC^C
ApaLI 1765 GTGCAC G^TGCAC
AspI 1221 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 2772 CCTAGG C^CTAGG
BamHI 1878 GGATCC G^GATCC
BbvCI 591 CCTCAGC CCTCAGC(-5/-2)
BlnI 2772 CCTAGG C^CTAGG
BseAI 842 TCCGGA T^CCGGA
BsgI 2842 CTGCAC GTGCAG(16/14)
Bsp1407I 2325 TGTACA T^GTACA
BspEI 842 TCCGGA T^CCGGA
BsrGI 2325 TGTACA T^GTACA
BstAPI 2844 GCACGAGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 465 GGTGACC G^GT[ACGT]ACC
BstPI 465 GGTGACC G^GT[ACGT]ACC
BstXI 2118 CCATGACAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1705 CCTAAGG CC^T[ACGT]AGG
DrdI 1183 GACTTTGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 2441 CCACGG C^C[GA][CT]GG
Eco81I 1705 CCTAAGG CC^T[ACGT]AGG
EcoO65I 465 GGTGACC G^GT[ACGT]ACC
EcoT14I 2772 CCTAGG C^C[AT][AT]GG
EcoT22I 2049 ATGCAT ATGCA^T
MaeII 2674 ACGT A^CGT
MroI 842 TCCGGA T^CCGGA
NaeI 2279 GCCGGC GCC^GGC
NgoMIV 2279 GCCGGC G^CCGGC
NruI 121 TCGCGA TCG^CGA
NsiI 2049 ATGCAT ATGCA^T
PflFI 1221 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 3125 ACCGGT A^CCGGT
PvuI 1216 CGATCG CGAT^CG
SalI 2742 GTCGAC G^TCGAC
SfiI 3101 GGCCCGTGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SspBI 2325 TGTACA T^GTACA
SspI 2069 AATATT AAT^ATT
StuI 2411 AGGCCT AGG^CCT
StyI 2772 CCTAGG C^C[AT][AT]GG
Tth111I 1221 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 33 enzymes) #
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AcsI 1324 AAATTT [GA]^AATT[CT]
1590 AAATTC
AflIII 503 ACATGT A^C[GA][CT]GT
959 ACATGT
AlwNI 1393 CAGACCCTG CAG[ACGT][ACGT][ACGT]^CTG
1652 CAGTCGCTG
ApoI 1324 AAATTT [GA]^AATT[CT]
1590 AAATTC
BalI 1097 TGGCCA TGG^CCA
1892 TGGCCA
BbeI 2886 GGCGCC GGCGC^C
3073 GGCGCC
BcgI 2169 CGAGGCTATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2985 CGACCCCGATGC
BfaI 2026 CTAG C^TAG
2773 CTAG
BsaWI 842 TCCGGA [AT]^CCGG[AT]
3125 ACCGGT
BsmI 909 GAATGC GAATGC(1/-1)
1420 GAATGC
BspDI 796 ATCGAT AT^CGAT
2104 ATCGAT
BspLU11I 503 ACATGT A^CATGT
959 ACATGT
BspMI 602 ACCTGC ACCTGC(4/8)
2746 ACCTGC
ClaI 796 ATCGAT AT^CGAT
2104 ATCGAT
DraI 1327 TTTAAA TTT^AAA
1665 TTTAAA
EagI 3109 CGGCCG C^GGCCG
3164 CGGCCG
EclXI 3109 CGGCCG C^GGCCG
3164 CGGCCG
Eco52I 3109 CGGCCG C^GGCCG
3164 CGGCCG
EcoRV 1642 GATATC GAT^ATC
1728 GATATC
HgaI 2522 GACGC GACGC(5/10)
3171 GACGC
HincII 2113 GTTGAC GT[CT]^[GA]AC
2742 GTCGAC
HindII 2113 GTTGAC GT[CT]^[GA]AC
2742 GTCGAC
KasI 2886 GGCGCC G^GCGCC
3073 GGCGCC
MaeI 2026 CTAG C^TAG
2773 CTAG
MluNI 1097 TGGCCA TGG^CCA
1892 TGGCCA
MscI 1097 TGGCCA TGG^CCA
1892 TGGCCA
NarI 2886 GGCGCC GG^CGCC
3073 GGCGCC
PaeR7I 181 CTCGAG C^TCGAG
2860 CTCGAG
PpuMI 1937 AGGACCC [GA]G^G[AT]CC[CT]
2031 GGGACCT
ScaI 185 AGTACT AGT^ACT
683 AGTACT
SfoI 2886 GGCGCC GGC^GCC
3073 GGCGCC
TfiI 928 GATTC G^A[AT]TC
1468 GATTC
XhoI 181 CTCGAG C^TCGAG
2860 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 115 enzymes) #
#########################################
AccII AciI AcyI AfaI AhdI
AluI AlwI Aor51HI Asp700I AspEI
AspHI AvaI AvaII BanI BanII
BbsI BbvI BcnI BglI BlpI
BmyI BpmI Bpu1102I BpuAI BsaHI
BsaI BsaJI BseRI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspHI BsrDI BsrFI BsrI
BssKI BssSI BstF5I BstNI BstUI
BstYI Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII EaeI
Eam1105I EarI Eco47III EcoO109I EcoRII
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PstI PvuII RcaI
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI SmlI TaqI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII XmaCI XmaI XmnI