Restriction map for
pF1KA0381
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# NO CUT ENZYMES ( 79 enzymes) #
##################################
AclI AflII AgeI Alw44I Aor51HI
ApaLI AscI AseI AsnI AviII
AvrII BbrPI BciVI BfrI BlnI
BlpI Bpu1102I BsaAI BsiWI Bsp1407I
BspDI BsrDI BsrGI BstBI BstEII
BstPI CelII ClaI CpoI DraI
DraIII EagI EclXI Eco47III Eco52I
EcoO65I EcoRV EcoT22I FseI FspI
HpaI KspI MfeI MseI MunI
NaeI NdeI NgoMIV NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RsrII SacII SalI SapI
SbfI ScaI SfiI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI Tru9I XhoI
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# ONE CUT ENZYMES ( 35 enzymes) #
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AatII 1768 GACGTC GACGT^C
Acc65I 797 GGTACC G^GTACC
AccI 923 GTATAC GT^[AC][GT]AC
Asp718I 797 GGTACC G^GTACC
AspI 1039 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 1898 GGATCC G^GATCC
BbvCI 2317 GCTGAGG CCTCAGC(-5/-2)
BclI 2534 TGATCA T^GATCA
BglI 20 GCCACCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 236 AGATCT A^GATCT
BsaBI 2535 GATCATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1334 GAGACC GGTCTC(1/5)
BsiEI 309 CGACCG CG[GA][CT]^CG
BsmBI 364 GAGACG CGTCTC(1/5)
BsmI 1402 GAATGC GAATGC(1/-1)
BspLU11I 576 ACATGT A^CATGT
BssHII 2965 GCGCGC G^CGCGC
BssSI 682 CTCGTG CACGAG(-5/-1)
Bst1107I 923 GTATAC GTA^TAC
BstZ17I 923 GTATAC GTA^TAC
DrdI 2719 GACAAGTTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FbaI 2534 TGATCA T^GATCA
HindIII 2065 AAGCTT A^AGCTT
KpnI 797 GGTACC GGTAC^C
MamI 2535 GATCATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 347 ACGCGT A^CGCGT
NheI 1023 GCTAGC G^CTAGC
PflFI 1039 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 781 GACCGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 288 CAGCTG CAG^CTG
SacI 139 GAGCTC GAGCT^C
TfiI 813 GAATC G^A[AT]TC
Tth111I 1039 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 2922 TCTAGA T^CTAGA
XcmI 2093 CCATCTTGAAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 36 enzymes) #
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AccII 348 CGCG CG^CG
2966 CGCG
AflIII 347 ACGCGT A^C[GA][CT]GT
576 ACATGT
AhdI 2488 GACACCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3073 GACCTGGTGTC
ApaI 1578 GGGCCC GGGCC^C
2030 GGGCCC
Asp700I 1815 GAAGTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2295 GAAGAAATTC
AspEI 2488 GACACCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3073 GACCTGGTGTC
AspHI 139 GAGCTC G[AT]GC[AT]^C
2191 GAGCAC
BalI 693 TGGCCA TGG^CCA
2465 TGGCCA
BbeI 2446 GGCGCC GGCGC^C
2696 GGCGCC
BcgI 1887 GCAGGTATTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3037 GCAGGCAGCTCG
BsgI 721 CTGCAC GTGCAG(16/14)
1138 CTGCAC
BsiHKAI 139 GAGCTC G[AT]GC[AT]^C
2191 GAGCAC
BspMI 1616 ACCTGC ACCTGC(4/8)
1887 GCAGGT
BsrFI 794 GCCGGT [GA]^CCGG[CT]
3191 ACCGGC
BstAPI 711 GCAGGCCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2268 GCAGCGACTGC
BstUI 348 CGCG CG^CG
2966 CGCG
BstXI 272 CCAACAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2342 CCATCCTGTTGG
Cfr10I 794 GCCGGT [GA]^CCGG[CT]
3191 ACCGGC
DsaI 24 CCATGG C^C[GA][CT]GG
323 CCATGG
Eam1105I 2488 GACACCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3073 GACCTGGTGTC
EcoRI 907 GAATTC G^AATTC
2866 GAATTC
HaeII 2446 GGCGCC [GA]GCGC^[CT]
2696 GGCGCC
HgaI 1443 GACGC GACGC(5/10)
2379 GCGTC
HincII 1294 GTCAAC GT[CT]^[GA]AC
2614 GTCAAC
HindII 1294 GTCAAC GT[CT]^[GA]AC
2614 GTCAAC
KasI 2446 GGCGCC G^GCGCC
2696 GGCGCC
MaeII 1769 ACGT A^CGT
3000 ACGT
MluNI 693 TGGCCA TGG^CCA
2465 TGGCCA
MscI 693 TGGCCA TGG^CCA
2465 TGGCCA
MvnI 348 CGCG CG^CG
2966 CGCG
NarI 2446 GGCGCC GG^CGCC
2696 GGCGCC
NcoI 24 CCATGG C^CATGG
323 CCATGG
SexAI 728 ACCAGGT A^CC[AT]GGT
3074 ACCTGGT
SfoI 2446 GGCGCC GGC^GCC
2696 GGCGCC
SmlI 2149 CTCAAG C^T[CT][GA]AG
3127 CTCAAG
XmnI 1815 GAAGTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2295 GAAGAAATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 108 enzymes) #
#########################################
AccIII AciI AcsI AcyI AfaI
AluI AlwI AlwNI AocI ApoI
AvaI AvaII BanI BanII BbsI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaHI BsaJI BsaWI BseAI
BseRI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspEI BspHI BsrBI
BsrI BssKI BstF5I BstNI BstYI
Bsu36I Cac8I CfoI Cfr13I DdeI
DpnII DraII EaeI EarI Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MflI MnlI MroI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI PstI RcaI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI StuI StyI TaqI TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoII XmaCI XmaI