Restriction map for
pF1KA0377
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# NO CUT ENZYMES ( 79 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AgeI Alw44I AlwNI ApaLI
AscI AseI AsnI Asp718I AviII
BbeI BciVI BfrI BsaHI BseAI
BsiEI BsiWI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrGI BssHII
BstAPI ClaI CpoI DraI DrdI
EagI EclXI Eco52I EcoNI EcoT22I
FseI FspI Hin1I HpaI KasI
KpnI KspI MroI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI PacI PinAI PmeI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SapI SfiI SfoI SgrAI
SmaI SnaBI SpeI SphI SspBI
SspI SwaI XmaCI XmaI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccII 2492 CGCG CG^CG
AhdI 2083 GACCCCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 3275 AGCGCT AGC^GCT
ApaI 2895 GGGCCC GGGCC^C
Asp700I 1970 GAAGTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 2083 GACCCCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 3042 CCTAGG C^CTAGG
BbrPI 3526 CACGTG CAC^GTG
BcgI 1038 CGATGACTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 3314 GCCATGTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1718 AGATCT A^GATCT
BlnI 3042 CCTAGG C^CTAGG
BsaBI 2640 GATTGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsrFI 2993 ACCGGC [GA]^CCGG[CT]
BssSI 2416 CACGAG CACGAG(-5/-1)
Bst1107I 780 GTATAC GTA^TAC
BstEII 3805 GGTAACC G^GT[ACGT]ACC
BstPI 3805 GGTAACC G^GT[ACGT]ACC
BstUI 2492 CGCG CG^CG
BstZ17I 780 GTATAC GTA^TAC
Cfr10I 2993 ACCGGC [GA]^CCGG[CT]
DraIII 1885 CACCGGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 2083 GACCCCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 3275 AGCGCT AGC^GCT
EcoO65I 3805 GGTAACC G^GT[ACGT]ACC
EcoRI 3127 GAATTC G^AATTC
FauI 1074 GCGGG CCCGC(4/6)
HincII 2431 GTCAAC GT[CT]^[GA]AC
HindII 2431 GTCAAC GT[CT]^[GA]AC
MamI 2640 GATTGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1471 CAATTG C^AATTG
MluI 2491 ACGCGT A^CGCGT
MunI 1471 CAATTG C^AATTG
MvnI 2492 CGCG CG^CG
PaeR7I 2976 CTCGAG C^TCGAG
PflMI 754 CCAACAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 3526 CACGTG CAC^GTG
PmlI 3526 CACGTG CAC^GTG
PshAI 252 GACTGTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SbfI 453 CCTGCAGG CCTGCA^GG
ScaI 1972 AGTACT AGT^ACT
SexAI 1374 ACCAGGT A^CC[AT]GGT
Sse8387I 453 CCTGCAGG CCTGCA^GG
Van91I 754 CCAACAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1785 TCTAGA T^CTAGA
XhoI 2976 CTCGAG C^TCGAG
XmnI 1970 GAAGTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 25 enzymes) #
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AccI 780 GTATAC GT^[AC][GT]AC
2095 GTAGAC
AcsI 3127 GAATTC [GA]^AATT[CT]
3519 AAATTC
AflIII 2491 ACGCGT A^C[GA][CT]GT
3525 ACACGT
ApoI 3127 GAATTC [GA]^AATT[CT]
3519 AAATTC
AspI 723 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2218 GACTGTGTC
AvaI 2456 CCCGAG C^[CT]CG[GA]G
2976 CTCGAG
BamHI 449 GGATCC G^GATCC
2673 GGATCC
BbvCI 1123 GCTGAGG CCTCAGC(-5/-2)
1603 GCTGAGG
BclI 2025 TGATCA T^GATCA
3504 TGATCA
BlpI 638 GCTCAGC GC^T[ACGT]AGC
3741 GCTCAGC
Bpu1102I 638 GCTCAGC GC^T[ACGT]AGC
3741 GCTCAGC
BsaAI 3239 CACGTA [CT]AC^GT[GA]
3526 CACGTG
BsaWI 446 ACCGGA [AT]^CCGG[AT]
1460 ACCGGA
BsmI 520 GAATGC GAATGC(1/-1)
2075 GAATGC
BsoBI 2456 CCCGAG C^[CT]CG[GA]G
2976 CTCGAG
BspMI 2099 ACCTGC ACCTGC(4/8)
2402 ACCTGC
BstBI 380 TTCGAA TT^CGAA
2486 TTCGAA
CelII 638 GCTCAGC GC^T[ACGT]AGC
3741 GCTCAGC
FbaI 2025 TGATCA T^GATCA
3504 TGATCA
HindIII 376 AAGCTT A^AGCTT
1499 AAGCTT
NspI 1631 GCATGT [GA]CATG^[CT]
3814 ACATGC
NspV 380 TTCGAA TT^CGAA
2486 TTCGAA
PflFI 723 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2218 GACTGTGTC
SfuI 380 TTCGAA TT^CGAA
2486 TTCGAA
Tth111I 723 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2218 GACTGTGTC
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# 3 OR MORE CUT ENZYMES ( 107 enzymes) #
#########################################
AciI AfaI AluI AlwI AocI
AspHI AvaII BalI BanI BanII
BbsI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI Bsp1286I BsrDI BsrI
BssKI BstF5I BstNI BstXI BstYI
Bsu36I Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
Eco81I EcoO109I EcoRII EcoRV EcoT14I
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NciI NcoI
NdeII NlaIII NlaIV PleI PpuMI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI XhoII