Restriction map for
pF1KA0351
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# NO CUT ENZYMES ( 106 enzymes) #
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AatII AccI AccII AccIII AcyI
AflII AflIII AgeI AhdI Alw44I
Aor51HI ApaI ApaLI AscI Asp700I
AspEI AviII BalI BbeI BbrPI
BcgI BciVI BfrI BlpI Bpu1102I
BsaAI BsaBI BsaHI BsaI BseAI
BsiEI BsmBI BsmI BspDI BspEI
BspHI BspLU11I BsrBI BssHII Bst1107I
BstUI BstXI BstZ17I CelII ClaI
CpoI DraIII EaeI EagI Eam1105I
EclXI Eco47III Eco52I EcoRI FseI
FspI HaeII HgaI Hin1I HincII
HindII HindIII HpaI KasI KspI
MamI MfeI MluI MluNI MroI
MscI MunI MvnI NaeI NarI
NdeI NgoMIV NotI NruI PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshAI PvuI RcaI RsrII SacII
SalI SbfI ScaI SfiI SfoI
SgrAI SmaI SnaBI SphI Sse8387I
SwaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 63 enzymes) #
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Acc65I 1644 GGTACC G^GTACC
AclI 268 AACGTT AA^CGTT
AcsI 323 AAATTC [GA]^AATT[CT]
AlwNI 1368 CAGGTACTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 323 AAATTC [GA]^AATT[CT]
AseI 177 ATTAAT AT^TAAT
AsnI 177 ATTAAT AT^TAAT
Asp718I 1644 GGTACC G^GTACC
AspI 1114 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1510 CCCGAG C^[CT]CG[GA]G
BamHI 1390 GGATCC G^GATCC
BbvCI 363 CCTCAGC CCTCAGC(-5/-2)
BclI 747 TGATCA T^GATCA
BcnI 284 CCCGG CC^[GC]GG
BglI 1247 GCCCCACCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 900 AGATCT A^GATCT
BsaWI 925 TCCGGT [AT]^CCGG[AT]
BsiWI 792 CGTACG C^GTACG
BsmAI 886 GTCTC GTCTC(1/5)
BsoBI 1510 CCCGAG C^[CT]CG[GA]G
Bsp1407I 2 TGTACA T^GTACA
BsrDI 1553 GCAATG GCAATG(2/0)
BsrFI 1252 ACCGGC [GA]^CCGG[CT]
BsrGI 2 TGTACA T^GTACA
BssSI 1359 CTCGTG CACGAG(-5/-1)
BstAPI 646 GCATATCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 710 TTCGAA TT^CGAA
BstEII 36 GGTCACC G^GT[ACGT]ACC
BstPI 36 GGTCACC G^GT[ACGT]ACC
CfoI 1351 GCGC GCG^C
Cfr10I 1252 ACCGGC [GA]^CCGG[CT]
DrdI 1111 GACGACAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 1298 CCATGG C^C[GA][CT]GG
EcoO65I 36 GGTCACC G^GT[ACGT]ACC
EcoRV 1522 GATATC GAT^ATC
EcoT22I 1619 ATGCAT ATGCA^T
FbaI 747 TGATCA T^GATCA
HhaI 1351 GCGC GCG^C
HinP1I 1351 GCGC G^CGC
KpnI 1644 GGTACC GGTAC^C
MaeII 269 ACGT A^CGT
NciI 284 CCCGG CC^[GC]GG
NcoI 1298 CCATGG C^CATGG
NsiI 1619 ATGCAT ATGCA^T
NspI 1621 GCATGT [GA]CATG^[CT]
NspV 710 TTCGAA TT^CGAA
PflFI 1114 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1367 CCAGGTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 909 AGGTCCC [GA]G^G[AT]CC[CT]
PshBI 177 ATTAAT AT^TAAT
Psp1406I 268 AACGTT AA^CGTT
PstI 738 CTGCAG CTGCA^G
SfcI 738 CTGCAG C^T[GA][CT]AG
SfuI 710 TTCGAA TT^CGAA
SmlI 1330 CTCAAG C^T[CT][GA]AG
SpeI 882 ACTAGT A^CTAGT
SspBI 2 TGTACA T^GTACA
SspI 703 AATATT AAT^ATT
StuI 1636 AGGCCT AGG^CCT
TspRI 1116 CAGTG CA[GC]TG[ACGT][ACGT]^
Tth111I 1114 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1367 CCAGGTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 396 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 15 enzymes) #
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AocI 656 CCTCAGG CC^T[ACGT]AGG
1639 CCTCAGG
AvaII 910 GGTCC G^G[AT]CC
1364 GGACC
AvrII 1026 CCTAGG C^CTAGG
1191 CCTAGG
BanI 1100 GGCACC G^G[CT][GA]CC
1644 GGTACC
BlnI 1026 CCTAGG C^CTAGG
1191 CCTAGG
BsgI 772 GTGCAG GTGCAG(16/14)
1492 GTGCAG
BspMI 1647 ACCTGC ACCTGC(4/8)
907 GCAGGT
Bsu36I 656 CCTCAGG CC^T[ACGT]AGG
1639 CCTCAGG
Eco81I 656 CCTCAGG CC^T[ACGT]AGG
1639 CCTCAGG
FauI 1130 CCCGC CCCGC(4/6)
1425 GCGGG
MslI 423 CATGTCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1609 CATTTGGATG
NheI 25 GCTAGC G^CTAGC
163 GCTAGC
SacI 808 GAGCTC GAGCT^C
1206 GAGCTC
SapI 805 GAAGAGC GCTCTTC(1/4)
1014 GAAGAGC
SexAI 296 ACCAGGT A^CC[AT]GGT
1366 ACCAGGT
#########################################
# 3 OR MORE CUT ENZYMES ( 74 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
BanII BbsI BbvI BfaI BmyI
BpmI BpuAI BsaJI BseRI BsiHKAI
BsiYI BslI BsmFI Bsp1286I BsrI
BssKI BstF5I BstNI BstYI Cac8I
Cfr13I DdeI DpnII DraI DraII
EarI EcoNI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MflI MnlI
MseI MspA1I MspI MvaI MwoI
NdeII NlaIII NlaIV PleI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TthHB8I XhoII