Restriction map for
pF1KA0339
##################################
# NO CUT ENZYMES ( 73 enzymes) #
##################################
AccI AclI AflII AgeI AhdI
Alw44I AocI ApaLI AscI AseI
AsnI AspEI AspI BbrPI BclI
BfrI BsmI Bsp1407I BspHI BspLU11I
BsrGI BssHII Bst1107I BstBI BstEII
BstPI BstZ17I Bsu36I DraI Eam1105I
Eco81I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI KspI MfeI MluI
MunI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI SacII
SalI ScaI SfuI SgrAI SpeI
SphI SspBI SspI SwaI Tth111I
XbaI XcmI XhoI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AatII 2804 GACGTC GACGT^C
Acc65I 4642 GGTACC G^GTACC
AcsI 2705 AAATTT [GA]^AATT[CT]
ApoI 2705 AAATTT [GA]^AATT[CT]
Asp700I 1360 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 4642 GGTACC G^GTACC
AviII 95 TGCGCA TGC^GCA
AvrII 3865 CCTAGG C^CTAGG
BcgI 4586 CGAACCGCGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2982 GGATAC GTATCC(6/5)
BfaI 3866 CTAG C^TAG
BglII 1751 AGATCT A^GATCT
BlnI 3865 CCTAGG C^CTAGG
BspDI 4897 ATCGAT AT^CGAT
BsrDI 4761 CATTGC GCAATG(2/0)
ClaI 4897 ATCGAT AT^CGAT
CpoI 4609 CGGTCCG CG^G[AT]CCG
EagI 638 CGGCCG C^GGCCG
EclXI 638 CGGCCG C^GGCCG
Eco52I 638 CGGCCG C^GGCCG
FspI 95 TGCGCA TGC^GCA
HindIII 741 AAGCTT A^AGCTT
HpaI 2058 GTTAAC GTT^AAC
KpnI 4642 GGTACC GGTAC^C
MaeI 3866 CTAG C^TAG
NaeI 1253 GCCGGC GCC^GGC
NcoI 4751 CCATGG C^CATGG
NgoMIV 1253 GCCGGC G^CCGGC
NspI 4823 ACATGC [GA]CATG^[CT]
PpuMI 4001 AGGTCCT [GA]G^G[AT]CC[CT]
RsrII 4609 CGGTCCG CG^G[AT]CCG
SacI 1990 GAGCTC GAGCT^C
SbfI 3352 CCTGCAGG CCTGCA^GG
SexAI 1317 ACCTGGT A^CC[AT]GGT
SfiI 3768 GGCCGACCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 4687 CTCAAG C^T[CT][GA]AG
SnaBI 4335 TACGTA TAC^GTA
Sse8387I 3352 CCTGCAGG CCTGCA^GG
XmnI 1360 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AccIII 4166 TCCGGA T^CCGGA
4694 TCCGGA
AflIII 818 ACACGT A^C[GA][CT]GT
4535 ACGTGT
BalI 780 TGGCCA TGG^CCA
4922 TGGCCA
BamHI 72 GGATCC G^GATCC
4727 GGATCC
BsaWI 4166 TCCGGA [AT]^CCGG[AT]
4694 TCCGGA
BseAI 4166 TCCGGA T^CCGGA
4694 TCCGGA
BsiEI 638 CGGCCG CG[GA][CT]^CG
1764 CGACCG
BsiWI 388 CGTACG C^GTACG
4337 CGTACG
BspEI 4166 TCCGGA T^CCGGA
4694 TCCGGA
BssSI 4732 CACGAG CACGAG(-5/-1)
2587 CTCGTG
DraIII 4250 CACGCAGTG CAC[ACGT][ACGT][ACGT]^GTG
4905 CACCAAGTG
EcoNI 1318 CCTGGTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3714 CCTCACCGAGG
HincII 145 GTCAAC GT[CT]^[GA]AC
2058 GTTAAC
HindII 145 GTCAAC GT[CT]^[GA]AC
2058 GTTAAC
MluNI 780 TGGCCA TGG^CCA
4922 TGGCCA
MroI 4166 TCCGGA T^CCGGA
4694 TCCGGA
MscI 780 TGGCCA TGG^CCA
4922 TGGCCA
MseI 242 TTAA T^TAA
2059 TTAA
PflMI 1312 CCAGAACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3406 CCACCTGCTGG
Tru9I 242 TTAA T^TAA
2059 TTAA
Van91I 1312 CCAGAACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3406 CCACCTGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 125 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI Aor51HI ApaI AspHI
AvaI AvaII BanI BanII BbeI
BbsI BbvCI BbvI BcnI BglI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaAI BsaBI BsaHI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrFI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DrdI
DsaI EaeI EarI Eco47III EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI KasI Ksp632I MaeII MaeIII
MamI MboI MboII MflI MnlI
MslI MspA1I MspI MvaI MvnI
MwoI NarI NciI NdeII NlaIII
NlaIV PleI PstI PvuII RsaI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SfoI SmaI StuI StyI
TaqI TfiI TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII XmaCI XmaI