Restriction map for
pF1KA0321
##################################
# NO CUT ENZYMES ( 54 enzymes) #
##################################
AatII AclI AgeI Alw44I ApaLI
AscI AseI AsnI AspI AviII
AvrII BbeI BlnI BsiEI BsiWI
Bsp1407I BspDI BspLU11I BsrGI BssHII
ClaI CpoI DraI EagI EclXI
Eco52I FseI FspI HpaI KasI
KspI MfeI MluI MunI NarI
NdeI NotI NruI PacI PflFI
PinAI PmeI PshBI Psp1406I PvuI
RsrII SacII SfiI SfoI SgrAI
SnaBI SspBI SwaI Tth111I
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
Acc65I 5427 GGTACC G^GTACC
AccII 2527 CGCG CG^CG
AccIII 293 TCCGGA T^CCGGA
AcyI 5492 GGCGTC G[GA]^CG[CT]C
AflIII 2281 ACACGT A^C[GA][CT]GT
Asp718I 5427 GGTACC G^GTACC
BamHI 4966 GGATCC G^GATCC
BcgI 6378 CGATTACTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 6361 GTATCC GTATCC(6/5)
BsaBI 5282 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 5492 GGCGTC G[GA]^CG[CT]C
BseAI 293 TCCGGA T^CCGGA
BsmBI 442 CGTCTC CGTCTC(1/5)
BspEI 293 TCCGGA T^CCGGA
BssSI 5582 CTCGTG CACGAG(-5/-1)
Bst1107I 5330 GTATAC GTA^TAC
BstBI 590 TTCGAA TT^CGAA
BstEII 5851 GGTCACC G^GT[ACGT]ACC
BstPI 5851 GGTCACC G^GT[ACGT]ACC
BstUI 2527 CGCG CG^CG
BstZ17I 5330 GTATAC GTA^TAC
DrdI 4498 GACACGGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 5851 GGTCACC G^GT[ACGT]ACC
EcoRV 133 GATATC GAT^ATC
EcoT22I 4409 ATGCAT ATGCA^T
Hin1I 5492 GGCGTC G[GA]^CG[CT]C
HindIII 301 AAGCTT A^AGCTT
KpnI 5427 GGTACC GGTAC^C
MamI 5282 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 293 TCCGGA T^CCGGA
MvnI 2527 CGCG CG^CG
NheI 921 GCTAGC G^CTAGC
NsiI 4409 ATGCAT ATGCA^T
NspV 590 TTCGAA TT^CGAA
SalI 3020 GTCGAC G^TCGAC
SbfI 737 CCTGCAGG CCTGCA^GG
SexAI 7021 ACCAGGT A^CC[AT]GGT
SfuI 590 TTCGAA TT^CGAA
SpeI 2014 ACTAGT A^CTAGT
SphI 6198 GCATGC GCATG^C
Sse8387I 737 CCTGCAGG CCTGCA^GG
SspI 6037 AATATT AAT^ATT
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccI 3020 GTCGAC GT^[AC][GT]AC
5330 GTATAC
AflII 3922 CTTAAG C^TTAAG
6877 CTTAAG
AhdI 435 GACTCCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3117 GACAGCCAGTC
Aor51HI 2612 AGCGCT AGC^GCT
2740 AGCGCT
AspEI 435 GACTCCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3117 GACAGCCAGTC
BbrPI 400 CACGTG CAC^GTG
6064 CACGTG
BclI 3522 TGATCA T^GATCA
5592 TGATCA
BfrI 3922 CTTAAG C^TTAAG
6877 CTTAAG
BlpI 777 GCTCAGC GC^T[ACGT]AGC
2382 GCTGAGC
Bpu1102I 777 GCTCAGC GC^T[ACGT]AGC
2382 GCTGAGC
BsrFI 5516 GCCGGC [GA]^CCGG[CT]
5977 GCCGGC
BstXI 2325 CCAGGCAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4908 CCACTTCTATGG
CelII 777 GCTCAGC GC^T[ACGT]AGC
2382 GCTGAGC
Cfr10I 5516 GCCGGC [GA]^CCGG[CT]
5977 GCCGGC
Eam1105I 435 GACTCCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3117 GACAGCCAGTC
Eco47III 2612 AGCGCT AGC^GCT
2740 AGCGCT
EcoRI 1081 GAATTC G^AATTC
7358 GAATTC
FbaI 3522 TGATCA T^GATCA
5592 TGATCA
HgaI 6420 GACGC GACGC(5/10)
5493 GCGTC
HincII 1676 GTCAAC GT[CT]^[GA]AC
3020 GTCGAC
HindII 1676 GTCAAC GT[CT]^[GA]AC
3020 GTCGAC
NaeI 5516 GCCGGC GCC^GGC
5977 GCCGGC
NcoI 2912 CCATGG C^CATGG
7596 CCATGG
NgoMIV 5516 GCCGGC G^CCGGC
5977 GCCGGC
PaeR7I 358 CTCGAG C^TCGAG
1003 CTCGAG
PmaCI 400 CACGTG CAC^GTG
6064 CACGTG
PmlI 400 CACGTG CAC^GTG
6064 CACGTG
PshAI 2896 GACCTCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
4926 GACTGCTGTC
ScaI 6137 AGTACT AGT^ACT
6707 AGTACT
StuI 734 AGGCCT AGG^CCT
4100 AGGCCT
XhoI 358 CTCGAG C^TCGAG
1003 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 131 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI AocI ApaI ApoI Asp700I
AspHI AvaI AvaII BalI BanI
BanII BbsI BbvCI BbvI BcnI
BfaI BglI BglII BmyI BpmI
BpuAI BsaAI BsaI BsaJI BsaWI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrI
BssKI BstAPI BstF5I BstNI BstYI
Bsu36I Cac8I CfoI Cfr13I DdeI
DpnII DraII DraIII DsaI EaeI
EarI Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI PstI PvuII RcaI
RsaI SacI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XbaI XcmI XhoII XmaCI XmaI
XmnI