Restriction map for
pF1KA0311
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# NO CUT ENZYMES ( 67 enzymes) #
##################################
Acc65I AccII AclI AgeI Alw44I
Aor51HI ApaLI AscI Asp718I AspI
BbeI BglI BsiEI BsiWI BsmBI
BspDI BspLU11I BsrBI BssHII BssSI
BstUI BstXI ClaI CpoI DrdI
EagI EclXI Eco47III Eco52I FseI
HaeII KasI KpnI KspI MluI
MvnI NarI NheI NotI NruI
PacI PaeR7I PflFI PflMI PinAI
PmeI Psp1406I PvuI RsrII SacII
SalI SbfI ScaI SfiI SfoI
SgrAI SmaI SnaBI SpeI Sse8387I
SwaI Tth111I Van91I XbaI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AatII 3398 GACGTC GACGT^C
AflII 1909 CTTAAG C^TTAAG
AhdI 2929 GACATGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 30 CCTGAGG CC^T[ACGT]AGG
ApaI 2143 GGGCCC GGGCC^C
AseI 3121 ATTAAT AT^TAAT
AsnI 3121 ATTAAT AT^TAAT
AspEI 2929 GACATGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 6429 CTCGGG C^[CT]CG[GA]G
AviII 4045 TGCGCA TGC^GCA
AvrII 3153 CCTAGG C^CTAGG
BbrPI 3203 CACGTG CAC^GTG
BciVI 2658 GGATAC GTATCC(6/5)
BfrI 1909 CTTAAG C^TTAAG
BlnI 3153 CCTAGG C^CTAGG
BlpI 1577 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1577 GCTCAGC GC^T[ACGT]AGC
BsaAI 3203 CACGTG [CT]AC^GT[GA]
BsaBI 301 GATGTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsoBI 6429 CTCGGG C^[CT]CG[GA]G
Bst1107I 2808 GTATAC GTA^TAC
BstAPI 498 GCAAGGTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 673 TTCGAA TT^CGAA
BstEII 6372 GGTCACC G^GT[ACGT]ACC
BstPI 6372 GGTCACC G^GT[ACGT]ACC
BstZ17I 2808 GTATAC GTA^TAC
Bsu36I 30 CCTGAGG CC^T[ACGT]AGG
CelII 1577 GCTCAGC GC^T[ACGT]AGC
Eam1105I 2929 GACATGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 30 CCTGAGG CC^T[ACGT]AGG
EcoO65I 6372 GGTCACC G^GT[ACGT]ACC
EcoRV 4966 GATATC GAT^ATC
FauI 483 GCGGG CCCGC(4/6)
FspI 4045 TGCGCA TGC^GCA
MamI 301 GATGTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1699 CAATTG C^AATTG
MunI 1699 CAATTG C^AATTG
NaeI 578 GCCGGC GCC^GGC
NgoMIV 578 GCCGGC G^CCGGC
NspV 673 TTCGAA TT^CGAA
PmaCI 3203 CACGTG CAC^GTG
PmlI 3203 CACGTG CAC^GTG
PshAI 685 GACTACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 3121 ATTAAT AT^TAAT
SfuI 673 TTCGAA TT^CGAA
SphI 4655 GCATGC GCATG^C
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# TWO CUT ENZYMES ( 28 enzymes) #
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AccIII 230 TCCGGA T^CCGGA
6766 TCCGGA
AcyI 511 GACGCC G[GA]^CG[CT]C
3398 GACGTC
BalI 77 TGGCCA TGG^CCA
5007 TGGCCA
BamHI 48 GGATCC G^GATCC
4485 GGATCC
BbvCI 2731 GCTGAGG CCTCAGC(-5/-2)
6664 GCTGAGG
BcnI 3450 CCGGG CC^[GC]GG
5133 CCCGG
BsaHI 511 GACGCC G[GA]^CG[CT]C
3398 GACGTC
BseAI 230 TCCGGA T^CCGGA
6766 TCCGGA
BspEI 230 TCCGGA T^CCGGA
6766 TCCGGA
BspMI 3209 ACCTGC ACCTGC(4/8)
5261 ACCTGC
BsrFI 578 GCCGGC [GA]^CCGG[CT]
6296 ACCGGC
Cfr10I 578 GCCGGC [GA]^CCGG[CT]
6296 ACCGGC
DraI 4326 TTTAAA TTT^AAA
4747 TTTAAA
DraIII 433 CACGCAGTG CAC[ACGT][ACGT][ACGT]^GTG
1378 CACAAGGTG
EaeI 77 TGGCCA [CT]^GGCC[GA]
5007 TGGCCA
EcoNI 239 CCTCAGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
5411 CCTGGATTAGG
EcoT22I 5144 ATGCAT ATGCA^T
6949 ATGCAT
HgaI 511 GACGC GACGC(5/10)
5160 GCGTC
Hin1I 511 GACGCC G[GA]^CG[CT]C
3398 GACGTC
HpaI 3 GTTAAC GTT^AAC
4216 GTTAAC
MluNI 77 TGGCCA TGG^CCA
5007 TGGCCA
MroI 230 TCCGGA T^CCGGA
6766 TCCGGA
MscI 77 TGGCCA TGG^CCA
5007 TGGCCA
NciI 3450 CCGGG CC^[GC]GG
5133 CCCGG
NdeI 2328 CATATG CA^TATG
5666 CATATG
NsiI 5144 ATGCAT ATGCA^T
6949 ATGCAT
SapI 946 GAAGAGC GCTCTTC(1/4)
5296 GAAGAGC
StuI 1865 AGGCCT AGG^CCT
6470 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI Asp700I
AspHI AvaII BanI BanII BbsI
BbvI BcgI BclI BfaI BglII
BmyI BpmI BpuAI BsaI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
Bsp1407I BspHI BsrDI BsrGI BsrI
BssKI BstF5I BstNI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EarI EcoO109I EcoRI EcoRII
EcoT14I FbaI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HincII HindII
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MwoI NcoI
NdeII NlaIII NlaIV NspI PleI
PpuMI PstI PvuII RcaI RsaI
SacI Sau3AI Sau96I ScrFI SexAI
SfaNI SfcI SmlI SspBI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoII XmnI