Restriction map for
pF1KA0310
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# NO CUT ENZYMES ( 46 enzymes) #
##################################
Acc65I AclI Aor51HI AscI Asp718I
AviII AvrII BglII BlnI BsaBI
BsmI BssHII BssSI Bst1107I BstZ17I
CpoI EagI EclXI Eco47III Eco52I
EcoRI EcoT22I FseI FspI HpaI
KpnI KspI MamI MfeI MluI
MunI NdeI NotI NsiI PacI
PmeI Psp1406I PvuI RsrII SacII
SbfI SgrAI SnaBI SpeI SphI
Sse8387I
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# ONE CUT ENZYMES ( 35 enzymes) #
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AccIII 5047 TCCGGA T^CCGGA
AflII 2991 CTTAAG C^TTAAG
AgeI 1836 ACCGGT A^CCGGT
AseI 4534 ATTAAT AT^TAAT
AsnI 4534 ATTAAT AT^TAAT
Asp700I 1387 GAATTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BfrI 2991 CTTAAG C^TTAAG
BseAI 5047 TCCGGA T^CCGGA
BsiEI 546 CGACCG CG[GA][CT]^CG
BsiWI 1036 CGTACG C^GTACG
Bsp1407I 3023 TGTACA T^GTACA
BspEI 5047 TCCGGA T^CCGGA
BspLU11I 6539 ACATGT A^CATGT
BsrGI 3023 TGTACA T^GTACA
BstBI 1831 TTCGAA TT^CGAA
BstEII 585 GGTCACC G^GT[ACGT]ACC
BstPI 585 GGTCACC G^GT[ACGT]ACC
DraI 6426 TTTAAA TTT^AAA
EcoO65I 585 GGTCACC G^GT[ACGT]ACC
EcoRV 3710 GATATC GAT^ATC
MroI 5047 TCCGGA T^CCGGA
NheI 79 GCTAGC G^CTAGC
NruI 5486 TCGCGA TCG^CGA
NspV 1831 TTCGAA TT^CGAA
PinAI 1836 ACCGGT A^CCGGT
PshBI 4534 ATTAAT AT^TAAT
SalI 3316 GTCGAC G^TCGAC
ScaI 3515 AGTACT AGT^ACT
SfiI 3148 GGCCAGCCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 1831 TTCGAA TT^CGAA
SspBI 3023 TGTACA T^GTACA
SspI 1803 AATATT AAT^ATT
SwaI 6425 ATTTAAAT ATTT^AAAT
XbaI 6547 TCTAGA T^CTAGA
XmnI 1387 GAATTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 19 enzymes) #
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AatII 5149 GACGTC GACGT^C
6583 GACGTC
AocI 3457 CCTCAGG CC^T[ACGT]AGG
6844 CCTGAGG
BbeI 3099 GGCGCC GGCGC^C
6093 GGCGCC
BbsI 2962 GAAGAC GAAGAC(2/6)
4604 GTCTTC
BclI 2499 TGATCA T^GATCA
5531 TGATCA
BpuAI 2962 GAAGAC GAAGAC(2/6)
4604 GTCTTC
BspDI 1696 ATCGAT AT^CGAT
6387 ATCGAT
Bsu36I 3457 CCTCAGG CC^T[ACGT]AGG
6844 CCTGAGG
ClaI 1696 ATCGAT AT^CGAT
6387 ATCGAT
DrdI 18 GACGGTCCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
577 GACGGTGTGGTC
Eco81I 3457 CCTCAGG CC^T[ACGT]AGG
6844 CCTGAGG
FbaI 2499 TGATCA T^GATCA
5531 TGATCA
HincII 3316 GTCGAC GT[CT]^[GA]AC
6580 GTTGAC
HindII 3316 GTCGAC GT[CT]^[GA]AC
6580 GTTGAC
KasI 3099 GGCGCC G^GCGCC
6093 GGCGCC
NaeI 4416 GCCGGC GCC^GGC
6618 GCCGGC
NarI 3099 GGCGCC GG^CGCC
6093 GGCGCC
NgoMIV 4416 GCCGGC G^CCGGC
6618 GCCGGC
SfoI 3099 GGCGCC GGC^GCC
6093 GGCGCC
#########################################
# 3 OR MORE CUT ENZYMES ( 158 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflIII AhdI AluI Alw44I
AlwI AlwNI ApaI ApaLI ApoI
AspEI AspHI AspI AvaI AvaII
BalI BamHI BanI BanII BbrPI
BbvCI BbvI BcgI BciVI BcnI
BfaI BglI BlpI BmyI BpmI
Bpu1102I BsaAI BsaHI BsaI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BsrI BssKI BstAPI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DraIII DsaI EaeI
Eam1105I EarI EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PaeR7I
PflFI PflMI PleI PmaCI PmlI
PpuMI PshAI PstI PvuII RcaI
RsaI SacI SapI Sau3AI Sau96I
ScrFI SexAI SfaNI SfcI SmaI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Tth111I TthHB8I Van91I XcmI XhoI
XhoII XmaCI XmaI