Restriction map for
pF1KA0305
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# NO CUT ENZYMES ( 107 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AgeI AhdI Alw44I Aor51HI
ApaI ApaLI AscI Asp718I AspEI
AspI AvaI AviII AvrII BamHI
BanI BbeI BbrPI BciVI BclI
BcnI BglI BlnI BlpI Bpu1102I
BsaHI BsaWI BseAI BsiEI BsiWI
BsmBI BsmI BsoBI BspDI BspEI
BsrBI BsrFI BssHII BstUI CelII
Cfr10I ClaI CpoI DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
FauI FbaI FseI FspI HaeII
HapII Hin1I HpaII KasI KpnI
KspI MluI MroI MspI MvnI
NaeI NarI NciI NgoMIV NotI
NruI PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI Psp1406I
PvuI RsrII SacII SalI SapI
SbfI SfiI SfoI SgrAI SmaI
SnaBI SpeI SphI Sse8387I StuI
SwaI Tth111I Van91I XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 41 enzymes) #
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Asp700I 3160 GAAGGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 3927 TGGCCA TGG^CCA
BanII 3974 GAGCTC G[GA]GC[CT]^C
BcgI 2296 CGACACCATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 692 AGATCT A^GATCT
BsaAI 4141 TACGTG [CT]AC^GT[GA]
BsaBI 3865 GATTCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 4051 GAGACC GGTCTC(1/5)
Bsp1407I 93 TGTACA T^GTACA
BspHI 3129 TCATGA T^CATGA
BspMI 907 ACCTGC ACCTGC(4/8)
BsrGI 93 TGTACA T^GTACA
BssSI 3202 CTCGTG CACGAG(-5/-1)
BstAPI 3607 GCATATCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 3695 TTCGAA TT^CGAA
BstXI 798 CCAGCCATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraIII 1178 CACGGGGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 3927 TGGCCA [CT]^GGCC[GA]
EcoRI 3524 GAATTC G^AATTC
EcoRV 4147 GATATC GAT^ATC
EcoT22I 101 ATGCAT ATGCA^T
HgaI 1138 GCGTC GACGC(5/10)
HindIII 3538 AAGCTT A^AGCTT
HpaI 2956 GTTAAC GTT^AAC
MaeII 4142 ACGT A^CGT
MamI 3865 GATTCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 3927 TGGCCA TGG^CCA
MscI 3927 TGGCCA TGG^CCA
NheI 4071 GCTAGC G^CTAGC
NsiI 101 ATGCAT ATGCA^T
NspV 3695 TTCGAA TT^CGAA
PshAI 2692 GACTTTAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 909 CTGCAG CTGCA^G
RcaI 3129 TCATGA T^CATGA
SacI 3974 GAGCTC GAGCT^C
ScaI 3804 AGTACT AGT^ACT
SexAI 2568 ACCAGGT A^CC[AT]GGT
SfuI 3695 TTCGAA TT^CGAA
SspBI 93 TGTACA T^GTACA
XbaI 2367 TCTAGA T^CTAGA
XmnI 3160 GAAGGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 33 enzymes) #
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AciI 866 CCGC CCGC(-3/-1)
4068 GCGG
AflII 1051 CTTAAG C^TTAAG
2461 CTTAAG
AocI 2503 CCTCAGG CC^T[ACGT]AGG
3331 CCTAAGG
AspHI 3974 GAGCTC G[AT]GC[AT]^C
4526 GTGCTC
BbvCI 1195 CCTCAGC CCTCAGC(-5/-2)
4498 CCTCAGC
BfrI 1051 CTTAAG C^TTAAG
2461 CTTAAG
BpmI 4046 CTCCAG CTGGAG(16/14)
3086 CTGGAG
BseRI 2501 CTCCTC GAGGAG(10/8)
3617 CTCCTC
BsgI 1022 CTGCAC GTGCAG(16/14)
3593 GTGCAG
BsiHKAI 3974 GAGCTC G[AT]GC[AT]^C
4526 GTGCTC
BstEII 1865 GGTTACC G^GT[ACGT]ACC
2873 GGTTACC
BstPI 1865 GGTTACC G^GT[ACGT]ACC
2873 GGTTACC
Bsu36I 2503 CCTCAGG CC^T[ACGT]AGG
3331 CCTAAGG
CfoI 1562 GCGC GCG^C
4381 GCGC
DraII 1988 AGGACCT [GA]G^G[ACGT]CC[CT]
4552 GGGACCT
Eco81I 2503 CCTCAGG CC^T[ACGT]AGG
3331 CCTAAGG
EcoO109I 1988 AGGACCT [GA]G^G[ACGT]CC[CT]
4552 GGGACCT
EcoO65I 1865 GGTTACC G^GT[ACGT]ACC
2873 GGTTACC
EcoT14I 4364 CCATGG C^C[AT][AT]GG
4545 CCATGG
HhaI 1562 GCGC GCG^C
4381 GCGC
HinP1I 1562 GCGC G^CGC
4381 GCGC
HincII 2956 GTTAAC GT[CT]^[GA]AC
3688 GTTGAC
HindII 2956 GTTAAC GT[CT]^[GA]AC
3688 GTTGAC
MfeI 713 CAATTG C^AATTG
1951 CAATTG
MspA1I 20 CAGCTG C[AC]G^C[GT]G
2081 CAGCTG
MunI 713 CAATTG C^AATTG
1951 CAATTG
NcoI 4364 CCATGG C^CATGG
4545 CCATGG
NdeI 104 CATATG CA^TATG
3742 CATATG
PpuMI 1988 AGGACCT [GA]G^G[AT]CC[CT]
4552 GGGACCT
PvuII 20 CAGCTG CAG^CTG
2081 CAGCTG
StyI 4364 CCATGG C^C[AT][AT]GG
4545 CCATGG
TaqI 747 TCGA T^CGA
3696 TCGA
TthHB8I 747 TCGA T^CGA
3696 TCGA
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# 3 OR MORE CUT ENZYMES ( 77 enzymes) #
#########################################
AccI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI AseI AsnI
AvaII BbsI BbvI BfaI BmyI
BpuAI BsaJI BsiYI BslI BsmAI
BsmFI Bsp1286I BspLU11I BsrDI BsrI
BssKI Bst1107I BstF5I BstNI BstYI
BstZ17I Cac8I Cfr13I DdeI DpnII
DraI DsaI EarI EcoRII Fnu4HI
FokI HaeIII HinfI HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MnlI MseI MslI MvaI
MwoI NdeII NlaIII NlaIV NspI
PleI PshBI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI XhoII