Restriction map for
pF1KA0266
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# NO CUT ENZYMES ( 127 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AgeI AhdI Alw44I Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
AvrII BanI BbeI BbrPI BbvCI
BciVI BcnI BglI BlnI BsaAI
BsaBI BsaWI BseAI BsiEI BsiWI
BsmBI Bsp1407I BspDI BspEI BspLU11I
BsrBI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstZ17I Cfr10I ClaI DraI
DraIII EagI Eam1105I EclXI Eco47III
Eco52I EcoO65I EcoRI EcoRV EcoT22I
FauI FseI FspI HaeII HapII
HincII HindII HpaI HpaII KasI
KpnI KspI MamI MfeI MluI
MroI MspI MunI MvnI NaeI
NarI NciI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
SacII SalI SbfI ScaI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SphI Sse8387I SspBI SspI SwaI
Tth111I Van91I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AcyI 1934 GACGCC G[GA]^CG[CT]C
AflII 886 CTTAAG C^TTAAG
AflIII 2272 ACACGT A^C[GA][CT]GT
AspHI 1297 GAGCTC G[AT]GC[AT]^C
AvaI 686 CTCGGG C^[CT]CG[GA]G
BamHI 2107 GGATCC G^GATCC
BbsI 2217 GTCTTC GAAGAC(2/6)
BcgI 1079 CGAATGAAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1667 TGATCA T^GATCA
BfrI 886 CTTAAG C^TTAAG
BlpI 1312 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1312 GCTGAGC GC^T[ACGT]AGC
BpuAI 2217 GTCTTC GAAGAC(2/6)
BsaHI 1934 GACGCC G[GA]^CG[CT]C
BsaI 1763 GAGACC GGTCTC(1/5)
BsiHKAI 1297 GAGCTC G[AT]GC[AT]^C
BsmI 394 GCATTC GAATGC(1/-1)
BsoBI 686 CTCGGG C^[CT]CG[GA]G
BspHI 1197 TCATGA T^CATGA
BsrDI 498 CATTGC GCAATG(2/0)
CelII 1312 GCTGAGC GC^T[ACGT]AGC
CpoI 1104 CGGACCG CG^G[AT]CCG
DsaI 614 CCATGG C^C[GA][CT]GG
FbaI 1667 TGATCA T^GATCA
HgaI 1934 GACGC GACGC(5/10)
Hin1I 1934 GACGCC G[GA]^CG[CT]C
MslI 1044 CACAGAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 463 CAGCTG C[AC]G^C[GT]G
NcoI 614 CCATGG C^CATGG
NspI 513 ACATGC [GA]CATG^[CT]
PshAI 1598 GACAGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 694 CTGCAG CTGCA^G
PvuII 463 CAGCTG CAG^CTG
RcaI 1197 TCATGA T^CATGA
RsrII 1104 CGGACCG CG^G[AT]CCG
SacI 1297 GAGCTC GAGCT^C
SexAI 11 ACCAGGT A^CC[AT]GGT
SpeI 51 ACTAGT A^CTAGT
StuI 623 AGGCCT AGG^CCT
TaqI 720 TCGA T^CGA
TthHB8I 720 TCGA T^CGA
XcmI 935 CCAAATATGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 20 enzymes) #
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AciI 171 GCGG CCGC(-3/-1)
1483 GCGG
AfaI 1516 GTAC GT^AC
2041 GTAC
AlwNI 522 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG
1125 CAGGAGCTG
AocI 1639 CCTAAGG CC^T[ACGT]AGG
1957 CCTGAGG
BglII 261 AGATCT A^GATCT
1291 AGATCT
BsgI 1131 CTGCAC GTGCAG(16/14)
1087 GTGCAG
BsmAI 1763 GAGAC GTCTC(1/5)
1698 GTCTC
BspMI 1467 ACCTGC ACCTGC(4/8)
2234 ACCTGC
Bsu36I 1639 CCTAAGG CC^T[ACGT]AGG
1957 CCTGAGG
CfoI 517 GCGC GCG^C
2013 GCGC
DrdI 1689 GACCACACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2233 GACCTGCCTGTC
Eco81I 1639 CCTAAGG CC^T[ACGT]AGG
1957 CCTGAGG
HhaI 517 GCGC GCG^C
2013 GCGC
HinP1I 517 GCGC G^CGC
2013 GCGC
HindIII 130 AAGCTT A^AGCTT
1787 AAGCTT
HphI 2162 TCACC GGTGA(8/7)
2267 TCACC
MaeII 1865 ACGT A^CGT
2274 ACGT
PleI 609 GACTC GAGTC(4/5)
323 GAGTC
RsaI 1516 GTAC GT^AC
2041 GTAC
SapI 1528 GAAGAGC GCTCTTC(1/4)
1537 GAAGAGC
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# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
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AcsI AluI AlwI ApoI AvaII
BalI BanII BbvI BfaI BmyI
BpmI BsaJI BseRI BsiYI BslI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI BstXI BstYI Cac8I Cfr13I
DdeI DpnII DraII EaeI EarI
EcoNI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeIII HinfI ItaI Ksp632I
MaeI MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MvaI
MwoI NdeII NlaIII NlaIV PpuMI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI XhoII