Restriction map for
pF1KA0234
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# NO CUT ENZYMES ( 71 enzymes) #
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Acc65I AccIII AflII AgeI AhdI
ApaI AscI AseI AsnI Asp718I
AspEI AspI AviII AvrII BamHI
BbeI BclI BfrI BlnI BseAI
BsiEI BsiWI BsmI BspDI BspEI
BspLU11I BssHII Bst1107I BstZ17I ClaI
CpoI DraIII DrdI EagI Eam1105I
EclXI Eco52I EcoRV FbaI FseI
FspI HpaI KasI KpnI MfeI
MluI MroI MunI NarI NotI
NruI PacI PaeR7I PflFI PinAI
PmeI PshBI PvuI RsrII SbfI
ScaI SfiI SfoI SgrAI SnaBI
SpeI SphI Sse8387I SwaI Tth111I
XhoI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AatII 1832 GACGTC GACGT^C
AclI 4186 AACGTT AA^CGTT
AflIII 668 ACGTGT A^C[GA][CT]GT
Alw44I 1795 GTGCAC G^TGCAC
Aor51HI 2732 AGCGCT AGC^GCT
ApaLI 1795 GTGCAC G^TGCAC
Asp700I 1065 GAAGGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 2696 CCCGGG C^[CT]CG[GA]G
BbrPI 1935 CACGTG CAC^GTG
BbvCI 2443 GCTGAGG CCTCAGC(-5/-2)
BcgI 3276 GCAGCTGCGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 3269 GTATCC GTATCC(6/5)
BglII 323 AGATCT A^GATCT
BsaBI 1774 GATCTCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 3212 GAGACC GGTCTC(1/5)
BsmBI 1765 GAGACG CGTCTC(1/5)
BsoBI 2696 CCCGGG C^[CT]CG[GA]G
Bsp1407I 1035 TGTACA T^GTACA
BsrBI 316 GAGCGG CCGCTC(-3/-3)
BsrGI 1035 TGTACA T^GTACA
BstBI 734 TTCGAA TT^CGAA
DraI 297 TTTAAA TTT^AAA
Eco47III 2732 AGCGCT AGC^GCT
EcoT22I 2389 ATGCAT ATGCA^T
KspI 146 CCGCGG CCGC^GG
MamI 1774 GATCTCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 1700 GCCGGC GCC^GGC
NdeI 1039 CATATG CA^TATG
NgoMIV 1700 GCCGGC G^CCGGC
NheI 3486 GCTAGC G^CTAGC
NsiI 2389 ATGCAT ATGCA^T
NspV 734 TTCGAA TT^CGAA
PmaCI 1935 CACGTG CAC^GTG
PmlI 1935 CACGTG CAC^GTG
PshAI 3397 GACCTGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 4186 AACGTT AA^CGTT
SacII 146 CCGCGG CCGC^GG
SalI 458 GTCGAC G^TCGAC
SexAI 1396 ACCTGGT A^CC[AT]GGT
SfuI 734 TTCGAA TT^CGAA
SmaI 2696 CCCGGG CCC^GGG
SspBI 1035 TGTACA T^GTACA
SspI 3817 AATATT AAT^ATT
XbaI 2931 TCTAGA T^CTAGA
XmaCI 2696 CCCGGG C^CCGGG
XmaI 2696 CCCGGG C^CCGGG
XmnI 1065 GAAGGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 23 enzymes) #
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AccII 147 CGCG CG^CG
202 CGCG
BalI 2309 TGGCCA TGG^CCA
2462 TGGCCA
BsaAI 1306 TACGTG [CT]AC^GT[GA]
1935 CACGTG
BsaWI 33 ACCGGA [AT]^CCGG[AT]
2039 TCCGGT
BspHI 1508 TCATGA T^CATGA
3986 TCATGA
BsrFI 1700 GCCGGC [GA]^CCGG[CT]
3137 GCCGGT
BstEII 2914 GGTGACC G^GT[ACGT]ACC
4011 GGTGACC
BstPI 2914 GGTGACC G^GT[ACGT]ACC
4011 GGTGACC
BstUI 147 CGCG CG^CG
202 CGCG
BstXI 557 CCAAAATGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3305 CCAGTCCACTGG
Cfr10I 1700 GCCGGC [GA]^CCGG[CT]
3137 GCCGGT
EcoO65I 2914 GGTGACC G^GT[ACGT]ACC
4011 GGTGACC
FauI 78 CCCGC CCCGC(4/6)
145 CCCGC
MluNI 2309 TGGCCA TGG^CCA
2462 TGGCCA
MscI 2309 TGGCCA TGG^CCA
2462 TGGCCA
MvnI 147 CGCG CG^CG
202 CGCG
NcoI 1533 CCATGG C^CATGG
3417 CCATGG
NspI 619 ACATGC [GA]CATG^[CT]
1028 ACATGC
PflMI 1211 CCAGTGGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3644 CCATTGGCTGG
RcaI 1508 TCATGA T^CATGA
3986 TCATGA
TaqI 459 TCGA T^CGA
735 TCGA
TthHB8I 459 TCGA T^CGA
735 TCGA
Van91I 1211 CCAGTGGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3644 CCATTGGCTGG
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# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AccI AciI AcsI AcyI AfaI
AluI AlwI AlwNI AocI ApoI
AspHI AvaII BanI BanII BbsI
BbvI BcnI BfaI BglI BlpI
BmyI BpmI Bpu1102I BpuAI BsaHI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BspMI
BsrDI BsrI BssKI BssSI BstAPI
BstF5I BstNI BstYI Bsu36I Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI Eco81I
EcoNI EcoO109I EcoRI EcoRII EcoT14I
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PleI
PpuMI PstI PvuII RsaI SacI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StuI StyI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
XcmI XhoII