Restriction map for
pF1KA0226
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# NO CUT ENZYMES ( 78 enzymes) #
##################################
AatII AccI AclI AflII AgeI
AhdI AscI AseI AsnI AspEI
AvrII BamHI BclI BfrI BlnI
BsaI BsiEI BsiWI BspHI BsrFI
BssHII Bst1107I BstBI BstXI BstZ17I
Cfr10I CpoI DraI DraIII EagI
Eam1105I EclXI Eco52I EcoRI EcoRV
FbaI FseI HincII HindII HpaI
MfeI MluI MunI NaeI NdeI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PinAI PmeI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SalI SapI SbfI SexAI SfiI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SspI StuI SwaI XbaI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 55 enzymes) #
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Acc65I 1232 GGTACC G^GTACC
AccIII 2042 TCCGGA T^CCGGA
AcyI 1490 GGCGCC G[GA]^CG[CT]C
AflIII 2327 ACATGT A^C[GA][CT]GT
Alw44I 133 GTGCAC G^TGCAC
AocI 923 CCTTAGG CC^T[ACGT]AGG
Aor51HI 2745 AGCGCT AGC^GCT
ApaLI 133 GTGCAC G^TGCAC
Asp700I 2325 GAACATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1232 GGTACC G^GTACC
AspI 2374 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 2153 TGCGCA TGC^GCA
BbeI 1490 GGCGCC GGCGC^C
BbvCI 1741 GCTGAGG CCTCAGC(-5/-2)
BcgI 1685 GCAAATCCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1050 GGATAC GTATCC(6/5)
BglI 2125 GCCCAGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 2130 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1490 GGCGCC G[GA]^CG[CT]C
BseAI 2042 TCCGGA T^CCGGA
BsmBI 612 CGTCTC CGTCTC(1/5)
Bsp1407I 1208 TGTACA T^GTACA
BspDI 376 ATCGAT AT^CGAT
BspEI 2042 TCCGGA T^CCGGA
BspLU11I 2327 ACATGT A^CATGT
BsrGI 1208 TGTACA T^GTACA
BstEII 1557 GGTCACC G^GT[ACGT]ACC
BstPI 1557 GGTCACC G^GT[ACGT]ACC
Bsu36I 923 CCTTAGG CC^T[ACGT]AGG
ClaI 376 ATCGAT AT^CGAT
DrdI 304 GACGCTCATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 2745 AGCGCT AGC^GCT
Eco81I 923 CCTTAGG CC^T[ACGT]AGG
EcoO65I 1557 GGTCACC G^GT[ACGT]ACC
EcoT22I 2505 ATGCAT ATGCA^T
FspI 2153 TGCGCA TGC^GCA
Hin1I 1490 GGCGCC G[GA]^CG[CT]C
HindIII 1548 AAGCTT A^AGCTT
KasI 1490 GGCGCC G^GCGCC
KpnI 1232 GGTACC GGTAC^C
KspI 2738 CCGCGG CCGC^GG
MamI 2130 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 2042 TCCGGA T^CCGGA
NarI 1490 GGCGCC GG^CGCC
NcoI 1754 CCATGG C^CATGG
NsiI 2505 ATGCAT ATGCA^T
PflFI 2374 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 568 CCACTCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacII 2738 CCGCGG CCGC^GG
SfoI 1490 GGCGCC GGC^GCC
SphI 2507 GCATGC GCATG^C
SspBI 1208 TGTACA T^GTACA
Tth111I 2374 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 568 CCACTCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 2325 GAACATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccII 2654 CGCG CG^CG
2739 CGCG
AcsI 271 AAATTC [GA]^AATT[CT]
1636 GAATTT
ApaI 1943 GGGCCC GGGCC^C
2455 GGGCCC
ApoI 271 AAATTC [GA]^AATT[CT]
1636 GAATTT
AvaI 247 CTCGGG C^[CT]CG[GA]G
815 CCCGAG
BbrPI 112 CACGTG CAC^GTG
1586 CACGTG
BbsI 1262 GAAGAC GAAGAC(2/6)
1441 GAAGAC
BglII 553 AGATCT A^GATCT
2213 AGATCT
BpuAI 1262 GAAGAC GAAGAC(2/6)
1441 GAAGAC
BsaAI 112 CACGTG [CT]AC^GT[GA]
1586 CACGTG
BsmAI 613 GTCTC GTCTC(1/5)
658 GTCTC
BsoBI 247 CTCGGG C^[CT]CG[GA]G
815 CCCGAG
BsrBI 412 CCGCTC CCGCTC(-3/-3)
2662 GAGCGG
BstAPI 201 GCACAGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2507 GCATGCTCTGC
BstUI 2654 CGCG CG^CG
2739 CGCG
HaeII 1490 GGCGCC [GA]GCGC^[CT]
2745 AGCGCT
HgaI 304 GACGC GACGC(5/10)
382 GCGTC
MvnI 2654 CGCG CG^CG
2739 CGCG
PmaCI 112 CACGTG CAC^GTG
1586 CACGTG
PmlI 112 CACGTG CAC^GTG
1586 CACGTG
SmlI 593 CTCAAG C^T[CT][GA]AG
1677 CTCAAG
TaqI 377 TCGA T^CGA
1382 TCGA
TthHB8I 377 TCGA T^CGA
1382 TCGA
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# 3 OR MORE CUT ENZYMES ( 102 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AspHI AvaII BalI BanI BanII
BbvI BcnI BfaI BlpI BmyI
BpmI Bpu1102I BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmFI
BsmI Bsp1286I BspMI BsrDI BsrI
BssKI BssSI BstF5I BstNI BstYI
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PpuMI PstI PvuII
RsaI SacI Sau3AI Sau96I ScaI
ScrFI SfaNI SfcI StyI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
XcmI XhoII