Restriction map for
pF1KA0164
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# NO CUT ENZYMES ( 125 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
AlwNI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AspHI
AviII AvrII BalI BbeI BbrPI
BbvCI BcgI BciVI BclI BcnI
BfrI BglI BlnI BlpI Bpu1102I
BsaAI BsaHI BseAI BsgI BsiEI
BsiHKAI BsiWI Bsp1407I BspEI BspLU11I
BsrGI BssHII Bst1107I BstAPI BstEII
BstPI BstZ17I CelII CpoI DraII
DrdI EaeI EagI Eam1105I EclXI
Eco47III Eco52I EcoO109I EcoO65I EcoT22I
FauI FbaI FseI FspI Hin1I
HincII HindII HindIII HpaI KasI
KpnI KspI MfeI MluI MluNI
MroI MscI MunI NaeI NarI
NciI NdeI NgoMIV NheI NotI
NsiI NspI PacI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshBI
Psp1406I PstI PvuI RsrII SacI
SacII SalI SbfI SexAI SfcI
SfiI SfoI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI Van91I XcmI XmaCI XmaI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AccI 1572 GTCTAC GT^[AC][GT]AC
AccII 175 CGCG CG^CG
AciI 1890 GCGG CCGC(-3/-1)
AocI 1983 CCTGAGG CC^T[ACGT]AGG
AspI 317 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 2288 CTCGAG C^[CT]CG[GA]G
AvaII 2452 GGTCC G^G[AT]CC
BamHI 2505 GGATCC G^GATCC
BanI 1653 GGCACC G^G[CT][GA]CC
BbsI 1441 GAAGAC GAAGAC(2/6)
BpmI 2139 CTCCAG CTGGAG(16/14)
BpuAI 1441 GAAGAC GAAGAC(2/6)
BsmBI 201 GAGACG CGTCTC(1/5)
BsoBI 2288 CTCGAG C^[CT]CG[GA]G
BspDI 595 ATCGAT AT^CGAT
BspHI 2664 TCATGA T^CATGA
BsrBI 1888 GAGCGG CCGCTC(-3/-3)
BsrDI 1873 GCAATG GCAATG(2/0)
BsrFI 389 GCCGGT [GA]^CCGG[CT]
BstBI 2399 TTCGAA TT^CGAA
BstUI 175 CGCG CG^CG
BstXI 2676 CCAAGGGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1983 CCTGAGG CC^T[ACGT]AGG
CfoI 2622 GCGC GCG^C
Cfr10I 389 GCCGGT [GA]^CCGG[CT]
ClaI 595 ATCGAT AT^CGAT
DraIII 1822 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 2711 CCATGG C^C[GA][CT]GG
Eco81I 1983 CCTGAGG CC^T[ACGT]AGG
EcoNI 313 CCTAGACGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1750 GAATTC G^AATTC
EcoRV 260 GATATC GAT^ATC
HaeII 2621 GGCGCT [GA]GCGC^[CT]
HgaI 2741 GACGC GACGC(5/10)
HhaI 2622 GCGC GCG^C
HinP1I 2622 GCGC G^CGC
MslI 265 CATCGAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 1868 CAGCTG C[AC]G^C[GT]G
MvnI 175 CGCG CG^CG
NcoI 2711 CCATGG C^CATGG
NruI 174 TCGCGA TCG^CGA
NspV 2399 TTCGAA TT^CGAA
PaeR7I 2288 CTCGAG C^TCGAG
PflFI 317 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 473 GACGAGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 1868 CAGCTG CAG^CTG
RcaI 2664 TCATGA T^CATGA
SapI 2417 GAAGAGC GCTCTTC(1/4)
SfuI 2399 TTCGAA TT^CGAA
StuI 1408 AGGCCT AGG^CCT
Tth111I 317 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 2288 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 24 enzymes) #
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Asp700I 122 GAACATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2532 GAAGTACTTC
BanII 1941 GAGCCC G[GA]GC[CT]^C
2136 GGGCTC
BbvI 1707 GCTGC GCAGC(8/12)
1870 GCTGC
BmyI 1941 GAGCCC G[AGT]GC[ACT]^C
2136 GGGCTC
BsaWI 625 TCCGGT [AT]^CCGG[AT]
1283 ACCGGA
BsiYI 313 CCTAGACGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
967 CCTGATGGTGG
BslI 313 CCTAGACGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
967 CCTGATGGTGG
BsmFI 2440 GGGAC GGGAC(10/14)
1535 GTCCC
BsmI 924 GAATGC GAATGC(1/-1)
780 GCATTC
Bsp1286I 1941 GAGCCC G[AGT]GC[ACT]^C
2136 GGGCTC
BspMI 1683 ACCTGC ACCTGC(4/8)
1494 GCAGGT
BssSI 1562 CACGAG CACGAG(-5/-1)
2384 CACGAG
Cac8I 1710 GCTTGC GC[ACGT]^[ACGT]GC
1789 GCCAGC
DraI 2023 TTTAAA TTT^AAA
2329 TTTAAA
Fnu4HI 1707 GCTGC GC^[ACGT]GC
1870 GCTGC
ItaI 1707 GCTGC GC^[ACGT]GC
1870 GCTGC
MaeII 330 ACGT A^CGT
2610 ACGT
MaeIII 1485 GTCAC ^GT[ACGT]AC
2063 GTGAC
MwoI 380 GCAGATCTCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
2615 GCAGAGGGCGC
ScaI 1927 AGTACT AGT^ACT
2534 AGTACT
SmlI 1306 CTCAAG C^T[CT][GA]AG
2288 CTCGAG
TseI 1707 GCTGC G^C[AT]GC
1870 GCTGC
Tsp45I 1485 GTCAC ^GT[GC]AC
2063 GTGAC
XmnI 122 GAACATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2532 GAAGTACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
BfaI BglII BsaBI BsaI BsaJI
BseRI BsmAI BsrI BssKI BstF5I
BstNI BstYI Cfr13I DdeI DpnII
EarI EcoRII EcoT14I FokI HaeIII
HapII HinfI HpaII HphI Ksp632I
MaeI MamI MboI MboII MflI
MnlI MseI MspI MvaI NdeII
NlaIII NlaIV PleI RsaI Sau3AI
Sau96I ScrFI SfaNI StyI TaqI
TfiI Tru9I Tsp509I TspRI TthHB8I
XbaI XhoII