Restriction map for
pF1KA0151
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# NO CUT ENZYMES ( 93 enzymes) #
##################################
Acc65I AccI AclI AflII AgeI
AhdI Aor51HI AscI AseI AsnI
Asp718I AspEI AspI AviII AvrII
BamHI BbrPI BcgI BciVI BclI
BfrI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsiEI BsiWI BspDI
BspHI BssHII Bst1107I BstAPI BstZ17I
CelII ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoRI EcoRV EcoT22I FbaI
FseI FspI HincII HindII HindIII
HpaI KpnI KspI MamI MfeI
MluI MseI MunI NcoI NdeI
NheI NotI NsiI PacI PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SexAI SfiI SgrAI
SnaBI SpeI SphI SspI SwaI
Tru9I Tth111I XbaI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AatII 1192 GACGTC GACGT^C
AccIII 91 TCCGGA T^CCGGA
AcsI 1737 GAATTT [GA]^AATT[CT]
AflIII 548 ACATGT A^C[GA][CT]GT
ApoI 1737 GAATTT [GA]^AATT[CT]
Asp700I 111 GAAGGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 842 TGGCCA TGG^CCA
BbeI 1258 GGCGCC GGCGC^C
BbvCI 1401 CCTCAGC CCTCAGC(-5/-2)
BglI 1865 GCCTGGTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BseAI 91 TCCGGA T^CCGGA
BsmI 306 GAATGC GAATGC(1/-1)
Bsp1407I 68 TGTACA T^GTACA
BspEI 91 TCCGGA T^CCGGA
BspLU11I 548 ACATGT A^CATGT
BsrBI 556 GAGCGG CCGCTC(-3/-3)
BsrDI 735 CATTGC GCAATG(2/0)
BsrGI 68 TGTACA T^GTACA
BssSI 1843 CACGAG CACGAG(-5/-1)
BstBI 569 TTCGAA TT^CGAA
BstEII 1066 GGTCACC G^GT[ACGT]ACC
BstPI 1066 GGTCACC G^GT[ACGT]ACC
BstXI 845 CCAACATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EcoO65I 1066 GGTCACC G^GT[ACGT]ACC
EcoT14I 1244 CCAAGG C^C[AT][AT]GG
KasI 1258 GGCGCC G^GCGCC
MaeII 1193 ACGT A^CGT
MluNI 842 TGGCCA TGG^CCA
MroI 91 TCCGGA T^CCGGA
MscI 842 TGGCCA TGG^CCA
NarI 1258 GGCGCC GG^CGCC
NruI 399 TCGCGA TCG^CGA
NspV 569 TTCGAA TT^CGAA
PaeR7I 1081 CTCGAG C^TCGAG
SacI 2053 GAGCTC GAGCT^C
ScaI 260 AGTACT AGT^ACT
SfoI 1258 GGCGCC GGC^GCC
SfuI 569 TTCGAA TT^CGAA
SmaI 482 CCCGGG CCC^GGG
SmlI 1081 CTCGAG C^T[CT][GA]AG
SspBI 68 TGTACA T^GTACA
StuI 1985 AGGCCT AGG^CCT
StyI 1244 CCAAGG C^C[AT][AT]GG
XhoI 1081 CTCGAG C^TCGAG
XmaCI 482 CCCGGG C^CCGGG
XmaI 482 CCCGGG C^CCGGG
XmnI 111 GAAGGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
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AccII 145 CGCG CG^CG
400 CGCG
AcyI 1192 GACGTC G[GA]^CG[CT]C
1258 GGCGCC
Alw44I 1015 GTGCAC G^TGCAC
1840 GTGCAC
AocI 322 CCTGAGG CC^T[ACGT]AGG
1159 CCTAAGG
ApaI 763 GGGCCC GGGCC^C
1289 GGGCCC
ApaLI 1015 GTGCAC G^TGCAC
1840 GTGCAC
AvaI 482 CCCGGG C^[CT]CG[GA]G
1081 CTCGAG
BbsI 115 GTCTTC GAAGAC(2/6)
937 GTCTTC
BfaI 1504 CTAG C^TAG
1679 CTAG
BpuAI 115 GTCTTC GAAGAC(2/6)
937 GTCTTC
BsaHI 1192 GACGTC G[GA]^CG[CT]C
1258 GGCGCC
BsaWI 91 TCCGGA [AT]^CCGG[AT]
704 ACCGGA
BsoBI 482 CCCGGG C^[CT]CG[GA]G
1081 CTCGAG
BstUI 145 CGCG CG^CG
400 CGCG
BstYI 606 GGATCT [GA]^GATC[CT]
1454 AGATCC
Bsu36I 322 CCTGAGG CC^T[ACGT]AGG
1159 CCTAAGG
DsaI 679 CCACGG C^C[GA][CT]GG
713 CCACGG
EaeI 359 TGGCCG [CT]^GGCC[GA]
842 TGGCCA
Eco81I 322 CCTGAGG CC^T[ACGT]AGG
1159 CCTAAGG
EcoNI 945 CCTGTCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1056 CCTCTTTGAGG
HaeII 475 GGCGCT [GA]GCGC^[CT]
1258 GGCGCC
HgaI 1091 GCGTC GACGC(5/10)
1804 GCGTC
Hin1I 1192 GACGTC G[GA]^CG[CT]C
1258 GGCGCC
MaeI 1504 CTAG C^TAG
1679 CTAG
MflI 606 GGATCT [GA]^GATC[CT]
1454 AGATCC
MspA1I 802 CAGCTG C[AC]G^C[GT]G
826 CAGCTG
MvnI 145 CGCG CG^CG
400 CGCG
PflMI 869 CCAAGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1690 CCAGGGCTTGG
PvuII 802 CAGCTG CAG^CTG
826 CAGCTG
SapI 1584 GAAGAGC GCTCTTC(1/4)
1939 GAAGAGC
SbfI 1221 CCTGCAGG CCTGCA^GG
1764 CCTGCAGG
Sse8387I 1221 CCTGCAGG CCTGCA^GG
1764 CCTGCAGG
TfiI 1626 GATTC G^A[AT]TC
1716 GATTC
Tsp509I 16 AATT ^AATT
1738 AATT
Van91I 869 CCAAGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1690 CCAGGGCTTGG
XcmI 636 CCATGCAGCCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1244 CCAAGGGCGTGTTGG
XhoII 606 GGATCT [GA]^GATC[CT]
1454 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 81 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AspHI AvaII BanI BanII BbvI
BcnI BmyI BpmI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI Bsp1286I BspMI
BsrFI BsrI BssKI BstF5I BstNI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII EarI EcoO109I EcoRII
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeIII MboI MboII
MnlI MslI MspI MvaI MwoI
NaeI NciI NdeII NgoMIV NlaIII
NlaIV NspI PleI PpuMI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI TaqI TseI Tsp45I TspRI
TthHB8I