Restriction map for
pF1KA0121
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# NO CUT ENZYMES ( 134 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AhdI Alw44I
AlwI AocI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AviII AvrII BalI
BamHI BbsI BciVI BfrI BglII
BlnI BlpI Bpu1102I BpuAI BsaBI
BseAI BsiEI BsiHKAI BsiWI BsmFI
BspDI BspEI BspHI BspLU11I BspMI
BsrDI BsrI BssSI Bst1107I BstBI
BstEII BstPI BstYI BstZ17I Bsu36I
CelII ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EarI EclXI
Eco52I Eco81I EcoO65I EcoRI EcoRV
EcoT22I FseI FspI HincII HindII
HindIII HpaI KpnI Ksp632I KspI
MamI MfeI MflI MluI MluNI
MroI MscI MseI MunI NcoI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflMI PinAI
PmeI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfcI SfiI SfuI SgrAI
SmlI SnaBI SpeI Sse8387I SspI
StuI SwaI TfiI Tru9I Van91I
XbaI XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AfaI 360 GTAC GT^AC
AflIII 740 ACACGT A^C[GA][CT]GT
Aor51HI 598 AGCGCT AGC^GCT
AspI 214 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 195 GGACC G^G[AT]CC
BbeI 511 GGCGCC GGCGC^C
BbrPI 741 CACGTG CAC^GTG
BbvCI 120 CCTCAGC CCTCAGC(-5/-2)
BclI 491 TGATCA T^GATCA
BfaI 199 CTAG C^TAG
BglI 341 GCCTGCACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 741 CACGTG [CT]AC^GT[GA]
BsaWI 584 ACCGGA [AT]^CCGG[AT]
BseRI 628 GAGGAG GAGGAG(10/8)
BsgI 343 CTGCAC GTGCAG(16/14)
BsmI 92 GAATGC GAATGC(1/-1)
Bsp1407I 359 TGTACA T^GTACA
BsrGI 359 TGTACA T^GTACA
BssHII 318 GCGCGC G^CGCGC
BstAPI 96 GCAGACCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 725 CCAAAGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraII 194 AGGACCT [GA]G^G[ACGT]CC[CT]
EaeI 761 CGGCCA [CT]^GGCC[GA]
Eco47III 598 AGCGCT AGC^GCT
EcoNI 797 CCTCTCGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 194 AGGACCT [GA]G^G[ACGT]CC[CT]
FbaI 491 TGATCA T^GATCA
KasI 511 GGCGCC G^GCGCC
MaeI 199 CTAG C^TAG
MboII 239 TCTTC GAAGA(8/7)
MslI 46 CATGCTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 552 CAGCGG C[AC]G^C[GT]G
NarI 511 GGCGCC GG^CGCC
NspI 45 GCATGC [GA]CATG^[CT]
PflFI 214 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 741 CACGTG CAC^GTG
PmlI 741 CACGTG CAC^GTG
PpuMI 194 AGGACCT [GA]G^G[AT]CC[CT]
RsaI 360 GTAC GT^AC
SfoI 511 GGCGCC GGC^GCC
SphI 45 GCATGC GCATG^C
SspBI 359 TGTACA T^GTACA
TaqI 314 TCGA T^CGA
Tsp509I 658 AATT ^AATT
Tth111I 214 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 314 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AlwNI 97 CAGACCCTG CAG[ACGT][ACGT][ACGT]^CTG
552 CAGCGGCTG
BanI 511 GGCGCC G^G[CT][GA]CC
678 GGCACC
BanII 667 GAGCCC G[GA]GC[CT]^C
702 GGGCTC
BcgI 714 CGACCACTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
305 GCAGCCCCATCG
BmyI 667 GAGCCC G[AGT]GC[ACT]^C
702 GGGCTC
BpmI 748 CTCCAG CTGGAG(16/14)
385 CTGGAG
BsaI 284 GAGACC GGTCTC(1/5)
864 GGTCTC
BsmBI 219 CGTCTC CGTCTC(1/5)
4 GAGACG
Bsp1286I 667 GAGCCC G[AGT]GC[ACT]^C
702 GGGCTC
BsrBI 295 GAGCGG CCGCTC(-3/-3)
466 GAGCGG
BstF5I 776 CATCC GGATG(2/0)
15 GGATG
DpnII 492 GATC ^GATC
753 GATC
DsaI 620 CCGTGG C^C[GA][CT]GG
707 CCGTGG
EcoT14I 378 CCTTGG C^C[AT][AT]GG
764 CCAAGG
FauI 515 CCCGC CCCGC(4/6)
814 CCCGC
FokI 776 CATCC GGATG(9/13)
15 GGATG
HaeII 511 GGCGCC [GA]GCGC^[CT]
598 AGCGCT
HgaI 6 GACGC GACGC(5/10)
421 GACGC
HinfI 231 GAGTC G^A[ACGT]TC
790 GAGTC
MaeII 218 ACGT A^CGT
742 ACGT
MboI 492 GATC ^GATC
753 GATC
NaeI 436 GCCGGC GCC^GGC
675 GCCGGC
NdeII 492 GATC ^GATC
753 GATC
NgoMIV 436 GCCGGC G^CCGGC
675 GCCGGC
PleI 231 GAGTC GAGTC(4/5)
790 GAGTC
Sau3AI 492 GATC ^GATC
753 GATC
StyI 378 CCTTGG C^C[AT][AT]GG
764 CCAAGG
XcmI 374 CCAGCCTTGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
407 CCAAGAACAGCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 50 enzymes) #
#########################################
AccII AciI AcyI AluI AvaI
BbvI BcnI BsaHI BsaJI BsiYI
BslI BsmAI BsoBI BsrFI BssKI
BstNI BstUI Cac8I CfoI Cfr10I
Cfr13I DdeI EcoRII Fnu4HI HaeIII
HapII HhaI Hin1I HinP1I HpaII
HphI ItaI MaeIII MnlI MspI
MvaI MvnI MwoI NciI NlaIII
NlaIV Sau96I ScrFI SfaNI SmaI
TseI Tsp45I TspRI XmaCI XmaI