Restriction map for
pF1KA0113
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# NO CUT ENZYMES ( 119 enzymes) #
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AatII Acc65I AccIII AclI AflII
AflIII AgeI AhdI Aor51HI AscI
AseI AsnI Asp718I AspEI AspI
AvaI AviII AvrII BamHI BbeI
BbrPI BbsI BbvCI BcgI BciVI
BclI BfrI BglI BlnI BpuAI
BsaAI BsaBI BseAI BsiEI BsiWI
BsmBI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstZ17I Cfr10I
ClaI CpoI DraI DraIII EagI
Eam1105I EclXI Eco47III Eco52I EcoRV
EcoT22I FbaI FseI FspI HincII
HindII HindIII HpaI KasI KpnI
KspI MaeII MamI MfeI MluI
MroI MseI MunI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI ScaI SexAI SfiI SfoI
SgrAI SmaI SnaBI SpeI SspBI
SspI SwaI TfiI Tru9I Tth111I
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 32 enzymes) #
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AccI 1796 GTCTAC GT^[AC][GT]AC
AccII 1763 CGCG CG^CG
AcyI 1793 GGCGTC G[GA]^CG[CT]C
Alw44I 592 GTGCAC G^TGCAC
ApaLI 592 GTGCAC G^TGCAC
Asp700I 1398 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BcnI 98 CCCGG CC^[GC]GG
BglII 1400 AGATCT A^GATCT
BsaHI 1793 GGCGTC G[GA]^CG[CT]C
BsgI 475 CTGCAC GTGCAG(16/14)
BsmI 1528 GCATTC GAATGC(1/-1)
BsrBI 1063 GAGCGG CCGCTC(-3/-3)
BsrDI 758 GCAATG GCAATG(2/0)
BstBI 1814 TTCGAA TT^CGAA
BstEII 1500 GGTCACC G^GT[ACGT]ACC
BstPI 1500 GGTCACC G^GT[ACGT]ACC
BstUI 1763 CGCG CG^CG
DrdI 277 GACTCAAATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 81 CCTAGTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1500 GGTCACC G^GT[ACGT]ACC
Hin1I 1793 GGCGTC G[GA]^CG[CT]C
MvnI 1763 CGCG CG^CG
NciI 98 CCCGG CC^[GC]GG
NspI 310 GCATGC [GA]CATG^[CT]
NspV 1814 TTCGAA TT^CGAA
PpuMI 866 AGGTCCC [GA]G^G[AT]CC[CT]
SbfI 1191 CCTGCAGG CCTGCA^GG
SfuI 1814 TTCGAA TT^CGAA
SmlI 739 CTCAAG C^T[CT][GA]AG
SphI 310 GCATGC GCATG^C
Sse8387I 1191 CCTGCAGG CCTGCA^GG
XmnI 1398 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 16 enzymes) #
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ApaI 441 GGGCCC GGGCC^C
1340 GGGCCC
BalI 1106 TGGCCA TGG^CCA
1730 TGGCCA
BanI 358 GGCACC G^G[CT][GA]CC
1674 GGTGCC
BlpI 259 GCTGAGC GC^T[ACGT]AGC
1203 GCTGAGC
Bpu1102I 259 GCTGAGC GC^T[ACGT]AGC
1203 GCTGAGC
BsaI 493 GAGACC GGTCTC(1/5)
1132 GAGACC
BsaWI 20 ACCGGA [AT]^CCGG[AT]
971 TCCGGT
BstXI 425 CCAATGCGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1727 CCATGGCCATGG
CelII 259 GCTGAGC GC^T[ACGT]AGC
1203 GCTGAGC
EcoRI 93 GAATTC G^AATTC
399 GAATTC
MluNI 1106 TGGCCA TGG^CCA
1730 TGGCCA
MscI 1106 TGGCCA TGG^CCA
1730 TGGCCA
PvuII 1147 CAGCTG CAG^CTG
1201 CAGCTG
SapI 1462 GAAGAGC GCTCTTC(1/4)
537 GCTCTTC
StuI 1586 AGGCCT AGG^CCT
1861 AGGCCT
XcmI 1115 CCAAGATTGAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1661 CCATGCCAGCCATGG
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# 3 OR MORE CUT ENZYMES ( 91 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI AocI ApoI AspHI AvaII
BanII BbvI BfaI BmyI BpmI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI BstYI Bsu36I
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI Eco81I
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MflI MnlI MslI MspA1I
MspI MvaI MwoI NcoI NdeII
NlaIII NlaIV PflMI PleI PstI
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TaqI TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoII