Restriction map for
pF1KA0110
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# NO CUT ENZYMES ( 153 enzymes) #
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AatII AccI AccIII AclI AcsI
AflIII AgeI AhdI Alw44I AlwI
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI AspEI AviII
AvrII BalI BamHI BanII BbrPI
BcgI BciVI BclI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BsaI
BsaWI BseAI BsgI BsiEI BsiWI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrFI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstYI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DpnII DraII
DraIII DrdI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRV EcoT22I
FauI FbaI FseI FspI HgaI
HincII HindII HindIII HpaI KspI
MaeII MamI MboI MfeI MflI
MluI MluNI MroI MscI MspA1I
MunI NaeI NcoI NdeI NdeII
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PinAI
PleI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI Sau3AI SbfI ScaI
SexAI SfiI SfuI SgrAI SmaI
SnaBI SphI Sse8387I SspBI SspI
SwaI Tsp509I XbaI XcmI XhoI
XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 48 enzymes) #
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Acc65I 165 GGTACC G^GTACC
AcyI 6 GGCGCC G[GA]^CG[CT]C
AflII 280 CTTAAG C^TTAAG
Asp700I 296 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 165 GGTACC G^GTACC
AspHI 543 GAGCAC G[AT]GC[AT]^C
AspI 729 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 338 CTCGGG C^[CT]CG[GA]G
AvaII 531 GGACC G^G[AT]CC
BbeI 6 GGCGCC GGCGC^C
BbsI 772 GAAGAC GAAGAC(2/6)
BcnI 708 CCGGG CC^[GC]GG
BfaI 436 CTAG C^TAG
BfrI 280 CTTAAG C^TTAAG
BglI 194 GCCAGGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 772 GAAGAC GAAGAC(2/6)
BsaHI 6 GGCGCC G[GA]^CG[CT]C
BsiHKAI 543 GAGCAC G[AT]GC[AT]^C
BsmBI 565 CGTCTC CGTCTC(1/5)
BsoBI 338 CTCGGG C^[CT]CG[GA]G
BsrDI 467 GCAATG GCAATG(2/0)
BstAPI 362 GCAGGAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 753 CATCC GGATG(2/0)
BstXI 578 CCATATTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraI 805 TTTAAA TTT^AAA
DsaI 761 CCACGG C^C[GA][CT]GG
EarI 568 CTCTTC CTCTTC(1/4)
FokI 753 CATCC GGATG(9/13)
HaeII 6 GGCGCC [GA]GCGC^[CT]
Hin1I 6 GGCGCC G[GA]^CG[CT]C
HinfI 453 GATTC G^A[ACGT]TC
HphI 354 GGTGA GGTGA(8/7)
KasI 6 GGCGCC G^GCGCC
KpnI 165 GGTACC GGTAC^C
Ksp632I 568 CTCTTC CTCTTC(1/4)
MaeI 436 CTAG C^TAG
NarI 6 GGCGCC GG^CGCC
NciI 708 CCGGG CC^[GC]GG
PflFI 729 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 761 CCACGGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SfcI 522 CTACAG C^T[GA][CT]AG
SfoI 6 GGCGCC GGC^GCC
SpeI 435 ACTAGT A^CTAGT
StuI 140 AGGCCT AGG^CCT
TfiI 453 GATTC G^A[AT]TC
Tth111I 729 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 761 CCACGGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 296 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 25 enzymes) #
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AccII 443 CGCG CG^CG
817 CGCG
AfaI 166 GTAC GT^AC
439 GTAC
AlwNI 38 CAGTCCCTG CAG[ACGT][ACGT][ACGT]^CTG
535 CAGAGCCTG
BbvCI 29 CCTCAGC CCTCAGC(-5/-2)
402 CCTCAGC
BpmI 391 CTGGAG CTGGAG(16/14)
412 CTGGAG
BseRI 614 CTCCTC GAGGAG(10/8)
316 GAGGAG
BsmFI 648 GGGAC GGGAC(10/14)
40 GTCCC
BstUI 443 CGCG CG^CG
817 CGCG
CfoI 7 GCGC GCG^C
561 GCGC
EcoT14I 459 CCTTGG C^C[AT][AT]GG
482 CCAAGG
HapII 10 CCGG C^CGG
708 CCGG
HhaI 7 GCGC GCG^C
561 GCGC
HinP1I 7 GCGC G^CGC
561 GCGC
HpaII 10 CCGG C^CGG
708 CCGG
MaeIII 727 GTGAC ^GT[ACGT]AC
799 GTGAC
MseI 281 TTAA T^TAA
806 TTAA
MslI 348 CACTGAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
579 CATATTCATG
MspI 10 CCGG C^CGG
708 CCGG
MvnI 443 CGCG CG^CG
817 CGCG
RsaI 166 GTAC GT^AC
439 GTAC
SfaNI 321 GATGC GCATC(5/9)
592 GATGC
SmlI 100 CTTGAG C^T[CT][GA]AG
280 CTTAAG
StyI 459 CCTTGG C^C[AT][AT]GG
482 CCAAGG
Tru9I 281 TTAA T^TAA
806 TTAA
Tsp45I 727 GTGAC ^GT[GC]AC
799 GTGAC
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# 3 OR MORE CUT ENZYMES ( 32 enzymes) #
#########################################
AciI AluI BanI BbvI BmyI
BsaJI BsiYI BslI BsmAI Bsp1286I
BsrI BssKI BstNI Cac8I Cfr13I
DdeI EcoRII Fnu4HI HaeIII ItaI
MboII MnlI MvaI MwoI NlaIII
NlaIV Sau96I ScrFI TaqI TseI
TspRI TthHB8I