Restriction map for
pF1KA0109
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# NO CUT ENZYMES ( 130 enzymes) #
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AatII Acc65I AccIII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AspI
AvrII BalI BbeI BbrPI BbvCI
BcgI BciVI BclI BfrI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BseRI
BsiEI BsiWI BsmBI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrFI BsrGI
BssSI Bst1107I BstEII BstPI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraII DraIII EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRV EcoT22I FbaI FseI
HaeII Hin1I HindIII HpaI KasI
KpnI KspI MamI MluI MluNI
MroI MscI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI RcaI RsrII
SacII SalI SbfI ScaI SexAI
SfiI SfoI SgrAI SmaI SnaBI
SpeI Sse8387I SspBI SspI StuI
SwaI Tth111I XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccI 55 GTCTAC GT^[AC][GT]AC
AfaI 1029 GTAC GT^AC
AlwNI 568 CAGGTGCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 387 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 50 CCCGAG C^[CT]CG[GA]G
AvaII 179 GGTCC G^G[AT]CC
AviII 128 TGCGCA TGC^GCA
BamHI 968 GGATCC G^GATCC
BanI 1173 GGTGCC G^G[CT][GA]CC
BbsI 232 GTCTTC GAAGAC(2/6)
BglI 1277 GCCGCAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 232 GTCTTC GAAGAC(2/6)
BsmAI 373 GAGAC GTCTC(1/5)
BsoBI 50 CCCGAG C^[CT]CG[GA]G
BsrBI 705 GAGCGG CCGCTC(-3/-3)
BsrDI 147 CATTGC GCAATG(2/0)
BssHII 380 GCGCGC G^CGCGC
BstBI 235 TTCGAA TT^CGAA
BstXI 459 CCAGGTAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 968 GGATCC [GA]^GATC[CT]
DraI 931 TTTAAA TTT^AAA
DrdI 1243 GACCATGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 227 CCATGG C^C[GA][CT]GG
EaeI 1275 TGGCCG [CT]^GGCC[GA]
FspI 128 TGCGCA TGC^GCA
HgaI 890 GACGC GACGC(5/10)
HincII 217 GTCAAC GT[CT]^[GA]AC
HindII 217 GTCAAC GT[CT]^[GA]AC
MfeI 719 CAATTG C^AATTG
MflI 968 GGATCC [GA]^GATC[CT]
MspA1I 686 CAGCTG C[AC]G^C[GT]G
MunI 719 CAATTG C^AATTG
NcoI 227 CCATGG C^CATGG
NspI 623 GCATGC [GA]CATG^[CT]
NspV 235 TTCGAA TT^CGAA
PvuII 686 CAGCTG CAG^CTG
RsaI 1029 GTAC GT^AC
SacI 514 GAGCTC GAGCT^C
SfcI 1236 CTACAG C^T[GA][CT]AG
SfuI 235 TTCGAA TT^CGAA
SmlI 1195 CTCAAG C^T[CT][GA]AG
SphI 623 GCATGC GCATG^C
XbaI 345 TCTAGA T^CTAGA
XcmI 182 CCAACATTTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 968 GGATCC [GA]^GATC[CT]
XmnI 387 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccII 381 CGCG CG^CG
1182 CGCG
AcsI 238 GAATTC [GA]^AATT[CT]
366 GAATTC
ApoI 238 GAATTC [GA]^AATT[CT]
366 GAATTC
BanII 514 GAGCTC G[GA]GC[CT]^C
1142 GGGCTC
BfaI 346 CTAG C^TAG
874 CTAG
BpmI 532 CTGGAG CTGGAG(16/14)
901 CTGGAG
BsaJI 227 CCATGG C^C[ACGT][ACGT]GG
839 CCAAGG
BsgI 735 CTGCAC GTGCAG(16/14)
997 GTGCAG
BsmFI 888 GGGAC GGGAC(10/14)
555 GTCCC
BsmI 631 GAATGC GAATGC(1/-1)
1044 GAATGC
BstAPI 82 GCAGTGGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
120 GCAGCAGGTGC
BstNI 459 CCAGG CC^[AT]GG
522 CCTGG
BstUI 381 CGCG CG^CG
1182 CGCG
Cfr13I 179 GGTCC G^G[ACGT]CC
591 GGGCC
EcoRI 238 GAATTC G^AATTC
366 GAATTC
EcoRII 459 CCAGG ^CC[AT]GG
522 CCTGG
EcoT14I 227 CCATGG C^C[AT][AT]GG
839 CCAAGG
MaeI 346 CTAG C^TAG
874 CTAG
MaeII 170 ACGT A^CGT
260 ACGT
MseI 173 TTAA T^TAA
932 TTAA
MslI 745 CACCAGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1155 CATTTCCATG
MvaI 459 CCAGG CC^[AT]GG
522 CCTGG
MvnI 381 CGCG CG^CG
1182 CGCG
NlaIV 968 GGATCC GG[ACGT]^[ACGT]CC
1173 GGTGCC
PflMI 182 CCAACATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1049 CCATCGTGTGG
Sau96I 179 GGTCC G^G[ACGT]CC
591 GGGCC
StyI 227 CCATGG C^C[AT][AT]GG
839 CCAAGG
TfiI 342 GATTC G^A[AT]TC
1087 GAATC
Tru9I 173 TTAA T^TAA
932 TTAA
Van91I 182 CCAACATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1049 CCATCGTGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AciI AluI AlwI AspHI BbvI
BcnI BmyI BsiHKAI BsiYI BslI
Bsp1286I BspMI BsrI BssKI BstF5I
Cac8I CfoI DdeI DpnII EarI
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeIII MboI MboII
MnlI MspI MwoI NciI NdeII
NlaIII PleI SapI Sau3AI ScrFI
SfaNI TaqI TseI Tsp45I Tsp509I
TspRI TthHB8I