Restriction map for
pF1KA0094
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# NO CUT ENZYMES ( 134 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI AlwI Aor51HI ApaI ApoI
AscI Asp700I Asp718I AspEI AspI
AvaI AviII AvrII BamHI BanI
BbeI BbrPI BbvCI BcgI BciVI
BfrI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BseRI BsgI BsiWI BsmI
BsoBI BspEI BspHI BspLU11I BsrBI
BssHII BssSI Bst1107I BstEII BstPI
BstYI BstZ17I CelII CfoI CpoI
DraIII DrdI Eam1105I EarI Eco47III
EcoNI EcoO65I EcoRI EcoT22I FseI
FspI HaeII HhaI Hin1I HinP1I
HincII HindII HpaI KasI KpnI
Ksp632I KspI MaeII MamI MflI
MluI MroI MspA1I NarI NheI
NotI NruI NsiI PacI PaeR7I
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PshAI Psp1406I PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SfiI
SfoI SgrAI SmaI SmlI SnaBI
SpeI Sse8387I SspI StuI SwaI
Tth111I Van91I XbaI XcmI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 63 enzymes) #
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AccII 17 CGCG CG^CG
Alw44I 1130 GTGCAC G^TGCAC
AlwNI 1091 CAGACACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 335 CCTTAGG CC^T[ACGT]AGG
ApaLI 1130 GTGCAC G^TGCAC
AseI 217 ATTAAT AT^TAAT
AsnI 217 ATTAAT AT^TAAT
AvaII 690 GGACC G^G[AT]CC
BalI 1018 TGGCCA TGG^CCA
BanII 93 GGGCTC G[GA]GC[CT]^C
BbsI 192 GTCTTC GAAGAC(2/6)
BclI 327 TGATCA T^GATCA
BcnI 1115 CCCGG CC^[GC]GG
BfaI 437 CTAG C^TAG
BglI 282 GCCAACAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 58 CTCCAG CTGGAG(16/14)
BpuAI 192 GTCTTC GAAGAC(2/6)
BsiEI 5 CGGCCG CG[GA][CT]^CG
BsiYI 229 CCATGGGCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 229 CCATGGGCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
Bsp1407I 507 TGTACA T^GTACA
BspDI 242 ATCGAT AT^CGAT
BspMI 235 GCAGGT ACCTGC(4/8)
BsrDI 674 GCAATG GCAATG(2/0)
BsrFI 460 GCCGGC [GA]^CCGG[CT]
BsrGI 507 TGTACA T^GTACA
BstAPI 517 GCATGTATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 863 TTCGAA TT^CGAA
BstUI 17 CGCG CG^CG
Bsu36I 335 CCTTAGG CC^T[ACGT]AGG
Cfr10I 460 GCCGGC [GA]^CCGG[CT]
ClaI 242 ATCGAT AT^CGAT
DraI 121 TTTAAA TTT^AAA
EagI 5 CGGCCG C^GGCCG
EclXI 5 CGGCCG C^GGCCG
Eco52I 5 CGGCCG C^GGCCG
Eco81I 335 CCTTAGG CC^T[ACGT]AGG
EcoRV 658 GATATC GAT^ATC
FauI 18 GCGGG CCCGC(4/6)
FbaI 327 TGATCA T^GATCA
HgaI 15 GACGC GACGC(5/10)
HindIII 892 AAGCTT A^AGCTT
MaeI 437 CTAG C^TAG
MfeI 974 CAATTG C^AATTG
MluNI 1018 TGGCCA TGG^CCA
MscI 1018 TGGCCA TGG^CCA
MslI 755 CATATGAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 974 CAATTG C^AATTG
MvnI 17 CGCG CG^CG
NaeI 460 GCCGGC GCC^GGC
NciI 1115 CCCGG CC^[GC]GG
NdeI 755 CATATG CA^TATG
NgoMIV 460 GCCGGC G^CCGGC
NlaIV 689 GGGACC GG[ACGT]^[ACGT]CC
NspV 863 TTCGAA TT^CGAA
PpuMI 689 GGGACCT [GA]G^G[AT]CC[CT]
PshBI 217 ATTAAT AT^TAAT
PstI 39 CTGCAG CTGCA^G
SexAI 474 ACCAGGT A^CC[AT]GGT
SfcI 39 CTGCAG C^T[GA][CT]AG
SfuI 863 TTCGAA TT^CGAA
SphI 834 GCATGC GCATG^C
SspBI 507 TGTACA T^GTACA
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# TWO CUT ENZYMES ( 21 enzymes) #
###################################
BsmBI 13 GAGACG CGTCTC(1/5)
1046 GAGACG
BsmFI 689 GGGAC GGGAC(10/14)
65 GTCCC
BstXI 840 CCAAGCAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1021 CCAGATGGTTGG
DpnII 328 GATC ^GATC
499 GATC
DraII 625 AGGCCCT [GA]G^G[ACGT]CC[CT]
689 GGGACCT
EaeI 5 CGGCCG [CT]^GGCC[GA]
1018 TGGCCA
EcoO109I 625 AGGCCCT [GA]G^G[ACGT]CC[CT]
689 GGGACCT
EcoT14I 229 CCATGG C^C[AT][AT]GG
606 CCATGG
HapII 461 CCGG C^CGG
1116 CCGG
HinfI 349 GAATC G^A[ACGT]TC
884 GAATC
HpaII 461 CCGG C^CGG
1116 CCGG
MboI 328 GATC ^GATC
499 GATC
MspI 461 CCGG C^CGG
1116 CCGG
NcoI 229 CCATGG C^CATGG
606 CCATGG
NdeII 328 GATC ^GATC
499 GATC
NspI 517 GCATGT [GA]CATG^[CT]
834 GCATGC
Sau3AI 328 GATC ^GATC
499 GATC
StyI 229 CCATGG C^C[AT][AT]GG
606 CCATGG
TaqI 243 TCGA T^CGA
864 TCGA
TfiI 349 GAATC G^A[AT]TC
884 GAATC
TthHB8I 243 TCGA T^CGA
864 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AciI AfaI AluI AspHI BbvI
BmyI BsaJI BsiHKAI BsmAI Bsp1286I
BsrI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DsaI EcoRII Fnu4HI
FokI HaeIII HphI ItaI MaeIII
MboII MnlI MseI MvaI MwoI
NlaIII RsaI Sau96I ScrFI SfaNI
Tru9I TseI Tsp45I Tsp509I TspRI