Restriction map for
pF1KA0090
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# NO CUT ENZYMES ( 91 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI Aor51HI AscI AseI AsnI
Asp718I AspI AviII AvrII BbrPI
BbvCI BcgI BciVI BfrI BlnI
BlpI Bpu1102I BsaBI BsaI BsaWI
BseAI BsiWI BspDI BspEI BspHI
BspLU11I BsrDI BsrFI BssHII Bst1107I
BstEII BstPI BstZ17I CelII Cfr10I
ClaI CpoI DraIII DrdI EarI
Eco47III EcoO65I EcoT22I FseI FspI
HpaI KpnI Ksp632I MamI MfeI
MluI MroI MslI MunI NaeI
NdeI NgoMIV NheI NruI NsiI
NspI PacI PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SalI SapI
SexAI SfiI SgrAI SnaBI SpeI
SphI SspI StuI SwaI Tth111I
XbaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AflIII 2882 ACGTGT A^C[GA][CT]GT
AhdI 1774 GACAAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1755 GTGCAC G^TGCAC
AlwNI 661 CAGCACCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 1755 GTGCAC G^TGCAC
Asp700I 1787 GAATGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1774 GACAAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 1571 TGGCCA TGG^CCA
BbeI 224 GGCGCC GGCGC^C
BglII 215 AGATCT A^GATCT
BsaAI 2881 TACGTG [CT]AC^GT[GA]
BsiEI 56 CGGCCG CG[GA][CT]^CG
Bsp1407I 2700 TGTACA T^GTACA
BsrGI 2700 TGTACA T^GTACA
BssSI 1132 CTCGTG CACGAG(-5/-1)
BstAPI 1869 GCAGTCCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 2054 TTCGAA TT^CGAA
DraI 1705 TTTAAA TTT^AAA
EagI 56 CGGCCG C^GGCCG
Eam1105I 1774 GACAAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 56 CGGCCG C^GGCCG
Eco52I 56 CGGCCG C^GGCCG
EcoNI 2613 CCTTCCTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1582 GAATTC G^AATTC
EcoRV 2045 GATATC GAT^ATC
FauI 2636 CCCGC CCCGC(4/6)
HincII 232 GTTGAC GT[CT]^[GA]AC
HindII 232 GTTGAC GT[CT]^[GA]AC
HindIII 1627 AAGCTT A^AGCTT
KasI 224 GGCGCC G^GCGCC
KspI 59 CCGCGG CCGC^GG
MluNI 1571 TGGCCA TGG^CCA
MscI 1571 TGGCCA TGG^CCA
NarI 224 GGCGCC GG^CGCC
NcoI 2534 CCATGG C^CATGG
NotI 55 GCGGCCGC GC^GGCCGC
NspV 2054 TTCGAA TT^CGAA
PaeR7I 2831 CTCGAG C^TCGAG
PvuII 2482 CAGCTG CAG^CTG
SacII 59 CCGCGG CCGC^GG
SbfI 405 CCTGCAGG CCTGCA^GG
ScaI 2378 AGTACT AGT^ACT
SfoI 224 GGCGCC GGC^GCC
SfuI 2054 TTCGAA TT^CGAA
Sse8387I 405 CCTGCAGG CCTGCA^GG
SspBI 2700 TGTACA T^GTACA
XcmI 2525 CCATCAGTGCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 2831 CTCGAG C^TCGAG
XmnI 1787 GAATGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 27 enzymes) #
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AccI 64 GTCTAC GT^[AC][GT]AC
2836 GTCTAC
AccII 60 CGCG CG^CG
2244 CGCG
AcyI 224 GGCGCC G[GA]^CG[CT]C
2287 GGCGTC
AocI 1619 CCTCAGG CC^T[ACGT]AGG
2617 CCTAAGG
ApaI 560 GGGCCC GGGCC^C
863 GGGCCC
BamHI 148 GGATCC G^GATCC
2631 GGATCC
BclI 290 TGATCA T^GATCA
2927 TGATCA
BglI 1200 GCCTGAGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2533 GCCATGGAGGC
BsaHI 224 GGCGCC G[GA]^CG[CT]C
2287 GGCGTC
BsmBI 1463 CGTCTC CGTCTC(1/5)
751 GAGACG
BsmI 2750 GAATGC GAATGC(1/-1)
2352 GCATTC
BsrBI 1188 GAGCGG CCGCTC(-3/-3)
1204 GAGCGG
BstUI 60 CGCG CG^CG
2244 CGCG
Bsu36I 1619 CCTCAGG CC^T[ACGT]AGG
2617 CCTAAGG
Eco81I 1619 CCTCAGG CC^T[ACGT]AGG
2617 CCTAAGG
FbaI 290 TGATCA T^GATCA
2927 TGATCA
HaeII 224 GGCGCC [GA]GCGC^[CT]
1856 AGCGCC
Hin1I 224 GGCGCC G[GA]^CG[CT]C
2287 GGCGTC
MvnI 60 CGCG CG^CG
2244 CGCG
PflMI 735 CCAAACTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1658 CCATCCTGTGG
PpuMI 824 GGGTCCT [GA]G^G[AT]CC[CT]
2492 AGGTCCT
SacI 720 GAGCTC GAGCT^C
2422 GAGCTC
SmaI 821 CCCGGG CCC^GGG
860 CCCGGG
SmlI 481 CTCAAG C^T[CT][GA]AG
2831 CTCGAG
Van91I 735 CCAAACTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1658 CCATCCTGTGG
XmaCI 821 CCCGGG C^CCGGG
860 CCCGGG
XmaI 821 CCCGGG C^CCGGG
860 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 91 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspHI AvaI AvaII BanI
BanII BbsI BbvI BcnI BfaI
BmyI BpmI BpuAI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI BstXI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PleI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII