Restriction map for
pF1KA0029
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# NO CUT ENZYMES ( 97 enzymes) #
##################################
AatII Acc65I AccII AccIII AflII
AflIII AgeI Alw44I AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspI BamHI BanII BbrPI
BbvCI BcgI BfrI BglI BsaBI
BseAI BsiEI BsiWI BsmBI BspDI
BspEI BspLU11I BsrBI BsrDI BsrFI
BssHII BssSI Bst1107I BstUI BstZ17I
Bsu36I Cfr10I ClaI CpoI DsaI
EagI EclXI Eco47III Eco52I Eco81I
EcoNI EcoT22I FseI HgaI KpnI
KspI MamI MfeI MluI MroI
MslI MunI MvnI NaeI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI PvuI RsrII SacI SacII
SalI SbfI SfiI SgrAI SnaBI
SpeI SphI Sse8387I StuI SwaI
Tth111I XhoI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AccI 2230 GTAGAC GT^[AC][GT]AC
AclI 623 AACGTT AA^CGTT
AlwNI 2419 CAGCCACTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 1498 GTGCTC G[AT]GC[AT]^C
AvaI 2383 CCCGGG C^[CT]CG[GA]G
AviII 1650 TGCGCA TGC^GCA
AvrII 1245 CCTAGG C^CTAGG
BbsI 610 GAAGAC GAAGAC(2/6)
BciVI 2403 GTATCC GTATCC(6/5)
BlnI 1245 CCTAGG C^CTAGG
BlpI 2302 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2302 GCTCAGC GC^T[ACGT]AGC
BpuAI 610 GAAGAC GAAGAC(2/6)
BsaAI 431 CACGTA [CT]AC^GT[GA]
BsaWI 2660 TCCGGT [AT]^CCGG[AT]
BsiHKAI 1498 GTGCTC G[AT]GC[AT]^C
BsoBI 2383 CCCGGG C^[CT]CG[GA]G
Bsp1407I 2068 TGTACA T^GTACA
BspHI 1367 TCATGA T^CATGA
BsrGI 2068 TGTACA T^GTACA
BstAPI 2809 GCACAGAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 974 TTCGAA TT^CGAA
BstEII 1981 GGTTACC G^GT[ACGT]ACC
BstPI 1981 GGTTACC G^GT[ACGT]ACC
CelII 2302 GCTCAGC GC^T[ACGT]AGC
DraI 2641 TTTAAA TTT^AAA
DraII 2672 GGGACCC [GA]G^G[ACGT]CC[CT]
DraIII 2090 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2233 GACAATATTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 2672 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoO65I 1981 GGTTACC G^GT[ACGT]ACC
EcoRI 1844 GAATTC G^AATTC
EcoRV 1463 GATATC GAT^ATC
FauI 2160 CCCGC CCCGC(4/6)
FspI 1650 TGCGCA TGC^GCA
HindIII 2623 AAGCTT A^AGCTT
NspV 974 TTCGAA TT^CGAA
PpuMI 2672 GGGACCC [GA]G^G[AT]CC[CT]
Psp1406I 623 AACGTT AA^CGTT
PstI 2425 CTGCAG CTGCA^G
RcaI 1367 TCATGA T^CATGA
SapI 1970 GCTCTTC GCTCTTC(1/4)
ScaI 926 AGTACT AGT^ACT
SfuI 974 TTCGAA TT^CGAA
SmaI 2383 CCCGGG CCC^GGG
SspBI 2068 TGTACA T^GTACA
XbaI 2368 TCTAGA T^CTAGA
XmaCI 2383 CCCGGG C^CCGGG
XmaI 2383 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AcyI 845 GGCGCC G[GA]^CG[CT]C
2650 GGCGCC
AhdI 2372 GACCTTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2674 GACCCCCAGTC
AspEI 2372 GACCTTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2674 GACCCCCAGTC
BalI 1746 TGGCCA TGG^CCA
2415 TGGCCA
BanI 845 GGCGCC G^G[CT][GA]CC
2650 GGCGCC
BbeI 845 GGCGCC GGCGC^C
2650 GGCGCC
BglII 321 AGATCT A^GATCT
883 AGATCT
BmyI 1498 GTGCTC G[AGT]GC[ACT]^C
2147 GTGCCC
BsaHI 845 GGCGCC G[GA]^CG[CT]C
2650 GGCGCC
BsaI 749 GAGACC GGTCTC(1/5)
1250 GGTCTC
BsgI 2449 CTGCAC GTGCAG(16/14)
2807 CTGCAC
BsmI 1919 GAATGC GAATGC(1/-1)
2814 GAATGC
Bsp1286I 1498 GTGCTC G[AGT]GC[ACT]^C
2147 GTGCCC
EaeI 1746 TGGCCA [CT]^GGCC[GA]
2415 TGGCCA
Eam1105I 2372 GACCTTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2674 GACCCCCAGTC
HaeII 845 GGCGCC [GA]GCGC^[CT]
2650 GGCGCC
Hin1I 845 GGCGCC G[GA]^CG[CT]C
2650 GGCGCC
HpaI 189 GTTAAC GTT^AAC
2842 GTTAAC
KasI 845 GGCGCC G^GCGCC
2650 GGCGCC
MluNI 1746 TGGCCA TGG^CCA
2415 TGGCCA
MscI 1746 TGGCCA TGG^CCA
2415 TGGCCA
NarI 845 GGCGCC GG^CGCC
2650 GGCGCC
PflMI 2089 CCACCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2407 CCATTACTTGG
SfoI 845 GGCGCC GGC^GCC
2650 GGCGCC
TaqI 975 TCGA T^CGA
2022 TCGA
Tsp45I 2696 GTCAC ^GT[GC]AC
2756 GTGAC
TthHB8I 975 TCGA T^CGA
2022 TCGA
Van91I 2089 CCACCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2407 CCATTACTTGG
XcmI 1698 CCAAGGAATAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2064 CCAATGTACAGCTGG
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# 3 OR MORE CUT ENZYMES ( 83 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI Asp700I AvaII BbvI BclI
BcnI BfaI BpmI BsaJI BseRI
BsiYI BslI BsmAI BsmFI BspMI
BsrI BssKI BstF5I BstNI BstXI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII EarI EcoRII EcoT14I FbaI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PleI
PvuII RsaI Sau3AI Sau96I ScrFI
SexAI SfaNI SfcI SmlI SspI
StyI TfiI Tru9I TseI Tsp509I
TspRI XhoII XmnI