Restriction map for
pF1KA0018
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# NO CUT ENZYMES ( 105 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AflII AgeI AhdI AocI ApaI
ApoI AscI AseI AsnI Asp718I
AspEI AspI AvrII BalI BamHI
BbvCI BciVI BfrI BlnI BsaWI
BseAI BsiEI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspLU11I BsrDI
BsrFI BsrGI Bst1107I BstBI BstXI
BstZ17I Bsu36I Cfr10I ClaI CpoI
DraI EagI Eam1105I EclXI Eco52I
Eco81I EcoRI EcoT22I FseI HincII
HindII HindIII HpaI KpnI KspI
MfeI MluI MluNI MroI MscI
MunI NaeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmeI PshBI Psp1406I
PvuI RsrII SacII SalI SbfI
ScaI SexAI SfiI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI TfiI Tth111I
XbaI XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AccI 1219 GTCTAC GT^[AC][GT]AC
AflIII 306 ACGTGT A^C[GA][CT]GT
AlwI 684 GATCC GGATC(4/5)
Asp700I 789 GAACCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 1510 CCCGAG C^[CT]CG[GA]G
AviII 1121 TGCGCA TGC^GCA
BbeI 278 GGCGCC GGCGC^C
BbrPI 1144 CACGTG CAC^GTG
BcgI 509 GCACAGGCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 1431 CCGGG CC^[GC]GG
BfaI 1267 CTAG C^TAG
BglI 1256 GCCAGCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 177 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 177 GCTCAGC GC^T[ACGT]AGC
BsaBI 684 GATCCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1114 GAGACC GGTCTC(1/5)
BseRI 1435 GAGGAG GAGGAG(10/8)
BsoBI 1510 CCCGAG C^[CT]CG[GA]G
BspMI 228 GCAGGT ACCTGC(4/8)
BsrBI 121 CCGCTC CCGCTC(-3/-3)
BsrI 730 CCAGT ACTGG(1/-1)
BstEII 408 GGTGACC G^GT[ACGT]ACC
BstPI 408 GGTGACC G^GT[ACGT]ACC
CelII 177 GCTCAGC GC^T[ACGT]AGC
DrdI 1210 GACATCCACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 408 GGTGACC G^GT[ACGT]ACC
EcoRV 139 GATATC GAT^ATC
FspI 1121 TGCGCA TGC^GCA
HapII 1431 CCGG C^CGG
HpaII 1431 CCGG C^CGG
KasI 278 GGCGCC G^GCGCC
MaeI 1267 CTAG C^TAG
MamI 684 GATCCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI 914 TTAA T^TAA
MslI 1158 CATGCTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 1431 CCGG C^CGG
NarI 278 GGCGCC GG^CGCC
NciI 1431 CCGGG CC^[GC]GG
NcoI 413 CCATGG C^CATGG
NdeI 1316 CATATG CA^TATG
PmaCI 1144 CACGTG CAC^GTG
PmlI 1144 CACGTG CAC^GTG
PpuMI 1351 AGGTCCT [GA]G^G[AT]CC[CT]
PshAI 840 GACAGGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 1180 CTGCAG CTGCA^G
PvuII 1366 CAGCTG CAG^CTG
SapI 36 GCTCTTC GCTCTTC(1/4)
SfcI 1180 CTGCAG C^T[GA][CT]AG
SfoI 278 GGCGCC GGC^GCC
SmlI 969 CTTGAG C^T[CT][GA]AG
StuI 1263 AGGCCT AGG^CCT
Tru9I 914 TTAA T^TAA
Tsp509I 892 AATT ^AATT
XmnI 789 GAACCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 29 enzymes) #
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AcyI 278 GGCGCC G[GA]^CG[CT]C
1501 GACGCC
Alw44I 270 GTGCAC G^TGCAC
1270 GTGCAC
Aor51HI 89 AGCGCT AGC^GCT
184 AGCGCT
ApaLI 270 GTGCAC G^TGCAC
1270 GTGCAC
BbsI 324 GAAGAC GAAGAC(2/6)
936 GAAGAC
BclI 344 TGATCA T^GATCA
500 TGATCA
BglII 1085 AGATCT A^GATCT
1526 AGATCT
BpuAI 324 GAAGAC GAAGAC(2/6)
936 GAAGAC
BsaHI 278 GGCGCC G[GA]^CG[CT]C
1501 GACGCC
BspHI 341 TCATGA T^CATGA
848 TCATGA
BssHII 29 GCGCGC G^CGCGC
156 GCGCGC
BssSI 199 CACGAG CACGAG(-5/-1)
769 CACGAG
DsaI 413 CCATGG C^C[GA][CT]GG
904 CCGTGG
EaeI 20 TGGCCG [CT]^GGCC[GA]
674 TGGCCG
EarI 37 CTCTTC CTCTTC(1/4)
109 CTCTTC
Eco47III 89 AGCGCT AGC^GCT
184 AGCGCT
EcoT14I 404 CCTTGG C^C[AT][AT]GG
413 CCATGG
FbaI 344 TGATCA T^GATCA
500 TGATCA
HgaI 657 GACGC GACGC(5/10)
1501 GACGC
Hin1I 278 GGCGCC G[GA]^CG[CT]C
1501 GACGCC
Ksp632I 37 CTCTTC CTCTTC(1/4)
109 CTCTTC
MaeIII 409 GTGAC ^GT[ACGT]AC
424 GTGAC
NspI 918 GCATGT [GA]CATG^[CT]
1157 ACATGC
PflMI 443 CCATTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
792 CCACTTCGTGG
RcaI 341 TCATGA T^CATGA
848 TCATGA
SacI 1012 GAGCTC GAGCT^C
1294 GAGCTC
StyI 404 CCTTGG C^C[AT][AT]GG
413 CCATGG
Tsp45I 409 GTGAC ^GT[GC]AC
424 GTGAC
Van91I 443 CCATTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
792 CCACTTCGTGG
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# 3 OR MORE CUT ENZYMES ( 70 enzymes) #
#########################################
AccII AciI AfaI AluI AlwNI
AspHI AvaII BanI BanII BbvI
BmyI BpmI BsaAI BsaJI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BssKI BstAPI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII DraIII EcoNI
EcoO109I EcoRII FauI Fnu4HI FokI
HaeII HaeIII HhaI HinP1I HinfI
HphI ItaI MaeII MboI MboII
MflI MnlI MspA1I MvaI MvnI
MwoI NdeII NlaIII NlaIV PleI
RsaI Sau3AI Sau96I ScrFI SfaNI
TaqI TseI TspRI TthHB8I XhoII