Restriction map for
pF1KB9855
##################################
# NO CUT ENZYMES ( 117 enzymes) #
##################################
AatII AccI AccIII AclI AcsI
AcyI AflII AgeI AhdI Alw44I
AlwNI AocI ApaLI ApoI AscI
AseI AsnI Asp700I AspEI AspHI
AspI AvrII BamHI BbeI BbrPI
BciVI BfaI BfrI BglII BlnI
BsaHI BsaWI BseAI BsiHKAI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspLU11I BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstZ17I
Bsu36I Cfr10I ClaI CpoI DraI
DrdI Eam1105I Eco81I EcoRI EcoRV
EcoT22I FseI HgaI Hin1I HincII
HindII HindIII HpaI KasI MaeI
MfeI MluI MroI MseI MunI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PflFI PinAI PleI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RsrII SacI SalI ScaI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SspBI SspI StuI SwaI
Tru9I Tsp509I Tth111I XbaI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 57 enzymes) #
###################################
Acc65I 74 GGTACC G^GTACC
AccII 1093 CGCG CG^CG
Aor51HI 938 AGCGCT AGC^GCT
Asp718I 74 GGTACC G^GTACC
AviII 1103 TGCGCA TGC^GCA
BbsI 507 GAAGAC GAAGAC(2/6)
BcgI 309 CGAGGGCACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 147 CCGGG CC^[GC]GG
BglI 674 GCCCCGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 319 GCTGAGC GC^T[ACGT]AGC
BpmI 1154 CTCCAG CTGGAG(16/14)
Bpu1102I 319 GCTGAGC GC^T[ACGT]AGC
BpuAI 507 GAAGAC GAAGAC(2/6)
BsaBI 441 GATCCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 535 GAGACC GGTCTC(1/5)
BsiEI 579 CGGCCG CG[GA][CT]^CG
BsmAI 535 GAGAC GTCTC(1/5)
BsmFI 6 GTCCC GGGAC(10/14)
BsrDI 212 CATTGC GCAATG(2/0)
BstUI 1093 CGCG CG^CG
BstXI 182 CCACGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 923 AGATCC [GA]^GATC[CT]
CelII 319 GCTGAGC GC^T[ACGT]AGC
DraIII 700 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 579 CGGCCG C^GGCCG
EarI 253 CTCTTC CTCTTC(1/4)
EclXI 579 CGGCCG C^GGCCG
Eco47III 938 AGCGCT AGC^GCT
Eco52I 579 CGGCCG C^GGCCG
FspI 1103 TGCGCA TGC^GCA
HapII 147 CCGG C^CGG
HinfI 1159 GATTC G^A[ACGT]TC
HpaII 147 CCGG C^CGG
KpnI 74 GGTACC GGTAC^C
Ksp632I 253 CTCTTC CTCTTC(1/4)
KspI 1092 CCGCGG CCGC^GG
MamI 441 GATCCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 923 AGATCC [GA]^GATC[CT]
MspI 147 CCGG C^CGG
MvnI 1093 CGCG CG^CG
NciI 147 CCGGG CC^[GC]GG
PaeR7I 841 CTCGAG C^TCGAG
PstI 166 CTGCAG CTGCA^G
PvuII 243 CAGCTG CAG^CTG
SacII 1092 CCGCGG CCGC^GG
SapI 252 GCTCTTC GCTCTTC(1/4)
SbfI 165 CCTGCAGG CCTGCA^GG
SexAI 701 ACCAGGT A^CC[AT]GGT
SfcI 166 CTGCAG C^T[GA][CT]AG
SphI 555 GCATGC GCATG^C
Sse8387I 165 CCTGCAGG CCTGCA^GG
TaqI 842 TCGA T^CGA
TfiI 1159 GATTC G^A[AT]TC
TthHB8I 842 TCGA T^CGA
XcmI 381 CCAAGTGACTATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 841 CTCGAG C^TCGAG
XhoII 923 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AfaI 75 GTAC GT^AC
658 GTAC
AflIII 173 ACGTGT A^C[GA][CT]GT
600 ACGTGT
AlwI 441 GATCC GGATC(4/5)
924 GATCC
ApaI 95 GGGCCC GGGCC^C
1096 GGGCCC
BbvCI 1085 CCTCAGC CCTCAGC(-5/-2)
958 GCTGAGG
BsaAI 34 TACGTG [CT]AC^GT[GA]
599 TACGTG
BseRI 418 GAGGAG GAGGAG(10/8)
961 GAGGAG
BspHI 470 TCATGA T^CATGA
503 TCATGA
BspMI 821 ACCTGC ACCTGC(4/8)
651 GCAGGT
BsrI 429 ACTGG ACTGG(1/-1)
1065 ACTGG
BstEII 292 GGTGACC G^GT[ACGT]ACC
607 GGTGACC
BstPI 292 GGTGACC G^GT[ACGT]ACC
607 GGTGACC
EcoNI 48 CCTGGCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
696 CCTCCACCAGG
EcoO65I 292 GGTGACC G^GT[ACGT]ACC
607 GGTGACC
HaeII 634 AGCGCC [GA]GCGC^[CT]
938 AGCGCT
NspI 555 GCATGC [GA]CATG^[CT]
647 ACATGC
PflMI 260 CCATGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
699 CCACCAGGTGG
PpuMI 56 AGGACCC [GA]G^G[AT]CC[CT]
836 AGGACCT
RcaI 470 TCATGA T^CATGA
503 TCATGA
RsaI 75 GTAC GT^AC
658 GTAC
SmlI 196 CTCAAG C^T[CT][GA]AG
841 CTCGAG
TspRI 315 CACTG CA[GC]TG[ACGT][ACGT]^
428 CACTG
Van91I 260 CCATGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
699 CCACCAGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 61 enzymes) #
#########################################
AciI AluI AvaI AvaII BalI
BanI BanII BbvI BclI BmyI
BsaJI BsgI BsiYI BslI BsoBI
Bsp1286I BssKI BstF5I BstNI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EcoO109I EcoRII EcoT14I
FauI FbaI Fnu4HI FokI HaeIII
HhaI HinP1I HphI ItaI MaeII
MaeIII MboI MboII MluNI MnlI
MscI MslI MspA1I MvaI MwoI
NcoI NdeII NlaIII NlaIV Sau3AI
Sau96I ScrFI SfaNI StyI TseI
Tsp45I