Restriction map for
pF1KSDF0187
(Circular) (Six-base) MAPSORT of: pF1KSDF0187.seq Check: 3737 from: 1 to: 4828
pF1KSDF0187 4828 bp
With 182 enzymes: SgfI *
June 20, 2008 10:36 ..
AccI GT'mk_AC
Cuts at: 1864 1864
Size: 4828
Acc65I G'GTAC_C
Cuts at: 1842 1842
Size: 4828
AclI AA'CG_TT
Cuts at: 4668 4668
Size: 4828
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 285 376 1182 1247 1350 1434 1518 2651 3083
Size: 91 806 65 103 84 84 1133 432
Cuts at: 3083 3832 4100 285
Size: 749 268 1013
Fragments arranged by size:
1133 1013 806 749 432 268 103 91
84 84 65
AflIII A'CryG_T
Cuts at: 233 2178 3284 4261 233
Size: 1945 1106 977 800
Fragments arranged by size:
1945 1106 977 800
AgeI A'CCGG_T
Cuts at: 3216 3216
Size: 4828
AhdI GACnn_n'nnGTC
Cuts at: 550 550
Size: 4828
AleI CACnn'nnGTG
Cuts at: 1259 1259
Size: 4828
AlwNI CAG_nnn'CTG
Cuts at: 378 411 861 2131 3700 378
Size: 33 450 1270 1569 1506
Fragments arranged by size:
1569 1506 1270 450 33
ApaI G_GGCC'C
Cuts at: 335 335
Size: 4828
ApaLI G'TGCA_C
Cuts at: 1680 3598 1680
Size: 1918 2910
ApoI r'AATT_y
Cuts at: 1568 1652 1830 4181 4437 1568
Size: 84 178 2351 256 1959
Fragments arranged by size:
2351 1959 256 178 84
AseI AT'TA_AT
Cuts at: 20 20
Size: 4828
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4828
AvaI C'yCGr_G
Cuts at: 335 624 1661 1846 335
Size: 289 1037 185 3317
Fragments arranged by size:
3317 1037 289 185
BamHI G'GATC_C
Cuts at: 606 1400 1851 606
Size: 794 451 3583
Fragments arranged by size:
3583 794 451
BanI G'GyrC_C
Cuts at: 597 1842 2256 2504 2539 597
Size: 1245 414 248 35 2886
Fragments arranged by size:
2886 1245 414 248 35
BanII G_rGCy'C
Cuts at: 335 461 708 1210 1546 1840 2870 335
Size: 126 247 502 336 294 1030 2293
Fragments arranged by size:
2293 1030 502 336 294 247 126
BbeI G_GCGC'C
Cuts at: 2260 2508 2260
Size: 248 4580
BbsI GAAGACnn'nnnn_
Cuts at: 233 527 233
Size: 294 4534
BciVI GTATCCnnnnn_n'
Cuts at: 2724 3487 4828 2724
Size: 763 1341 2724
Fragments arranged by size:
2724 1341 763
BclI T'GATC_A
Cuts at: 2346 2346
Size: 4828
BfrBI ATG'CAT
Cuts at: 1786 1786
Size: 4828
BglII A'GATC_T
Cuts at: 2341 4380 2341
Size: 2039 2789
BlpI GC'TnA_GC
Cuts at: 1938 1938
Size: 4828
Bme1580I G_kGCm'C
Cuts at: 335 1684 2451 2544 3602 335
Size: 1349 767 93 1058 1561
Fragments arranged by size:
1561 1349 1058 767 93
BmrI ACTGGGnnnn_n'
Cuts at: 648 2038 2119 2454 4600 648
Size: 1390 81 335 2146 876
Fragments arranged by size:
2146 1390 876 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 361 975 1773 3193 361
Size: 614 798 1420 1996
Fragments arranged by size:
1996 1420 798 614
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 257 433 445 859 1346 1514 257
Size: 176 12 414 487 168 3571
Fragments arranged by size:
3571 487 414 176 168 12
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2002 2881 3375 3673 2002
Size: 879 494 298 3157
Fragments arranged by size:
3157 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 749 4532 749
Size: 3783 1045
BsaAI yAC'GTr
Cuts at: 1818 2179 1818
Size: 361 4467
BsaBI GATnn'nnATC
Cuts at: 6 2340 4379 6
Size: 2334 2039 455
Fragments arranged by size:
2334 2039 455
BsaHI Gr'CG_yC
Cuts at: 2257 2505 3209 4705 2257
Size: 248 704 1496 2380
Fragments arranged by size:
2380 1496 704 248
BsaWI w'CCGG_w
Cuts at: 2015 2536 3216 3490 3637 4048 2015
Size: 521 680 274 147 411 2795
Fragments arranged by size:
2795 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 255 285 255
Size: 30 4798
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 260 514 964 1099 260
Size: 254 450 135 3989
Fragments arranged by size:
3989 450 254 135
BseYI C'CCAG_C
Cuts at: 1770 2251 3009 3588 4084 1770
Size: 481 758 579 496 2514
Fragments arranged by size:
2514 758 579 496 481
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 158 713 758 158
Size: 555 45 4228
Fragments arranged by size:
4228 555 45
BsiEI CG_ry'CG
Cuts at: 104 2368 2414 3624 104
Size: 2264 46 1210 1308
Fragments arranged by size:
2264 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 461 708 1210 1546 1684 1840 2618 2808 3602
Size: 247 502 336 138 156 778 190 794
Cuts at: 3602 461
Size: 1687
Fragments arranged by size:
1687 794 778 502 336 247 190 156
138
BsmBI CGTCTCn'nnnn_
Cuts at: 680 680
Size: 4828
Bsp1286I G_dGCh'C
Cuts at: 335 461 708 1210 1546 1684 1840 2451 2544
Size: 126 247 502 336 138 156 611 93
Cuts at: 2544 2618 2808 2870 3602 335
Size: 74 190 62 732 1561
Fragments arranged by size:
1561 732 611 502 336 247 190 156
138 126 93 74 62
BspEI T'CCGG_A
Cuts at: 4048 4048
Size: 4828
BspHI T'CATG_A
Cuts at: 738 1422 4004 738
Size: 684 2582 1562
Fragments arranged by size:
2582 1562 684
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1876 2090 2392 2773 1876
Size: 214 302 381 3931
Fragments arranged by size:
3931 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3118 3172 4658 4823 50
Size: 3068 54 1486 165 55
Fragments arranged by size:
3068 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 806 2738 806
Size: 1932 2896
BsrFI r'CCGG_y
Cuts at: 2824 3005 3216 2824
Size: 181 211 4436
Fragments arranged by size:
4436 211 181
BssSI C'ACGA_G
Cuts at: 643 701 1284 3097 3457 643
Size: 58 583 1813 360 2014
Fragments arranged by size:
2014 1813 583 360 58
BstAPI GCAn_nnn'nTGC
Cuts at: 2203 2203
Size: 4828
BstBI TT'CG_AA
Cuts at: 110 110
Size: 4828
BstYI r'GATC_y
Cuts at: 1 407 606 1012 1400 1851 2318 2341 2676
Size: 406 199 406 388 451 467 23 335
Cuts at: 2676 2922 3936 4022 4034 4380 1
Size: 246 1014 86 12 346 449
Fragments arranged by size:
1014 467 451 449 406 406 388 346
335 246 199 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1082 1082
Size: 4828
BtgI C'CryG_G
Cuts at: 152 1238 152
Size: 1086 3742
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 778 2776 2957 3120 778
Size: 1998 181 163 2486
Fragments arranged by size:
2486 1998 181 163
BtsI GCAGTG_nn'
Cuts at: 2069 2069
Size: 4828
DraI TTT'AAA
Cuts at: 1825 1825
Size: 4828
DraIII CAC_nnn'GTG
Cuts at: 1688 1688
Size: 4828
DrdI GACnn_nn'nnGTC
Cuts at: 2532 3392 2532
Size: 860 3968
EaeI y'GGCC_r
Cuts at: 382 2411 2585 2976 3003 4350 382
Size: 2029 174 391 27 1347 860
Fragments arranged by size:
2029 1347 860 391 174 27
EagI C'GGCC_G
Cuts at: 2411 2411
Size: 4828
EarI CTCTTCn'nnn_
Cuts at: 206 713 2849 3059 206
Size: 507 2136 210 1975
Fragments arranged by size:
2136 1975 507 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 280 1294 3034 3346 3492 4638 280
Size: 1014 1740 312 146 1146 470
Fragments arranged by size:
1740 1146 1014 470 312 146
EcoICRI GAG'CTC
Cuts at: 459 706 1208 1544 1838 459
Size: 247 502 336 294 3449
Fragments arranged by size:
3449 502 336 294 247
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 257 285 376 433 445 859 1182 1247 1346
Size: 28 91 57 12 414 323 65 99
Cuts at: 1346 1350 1434 1514 1518 2651 3083 3832 4100
Size: 4 84 80 4 1133 432 749 268
Cuts at: 4100 257
Size: 985
Fragments arranged by size:
1133 985 749 432 414 323 268 99
91 84 80 65 57 28 12 4
4
EcoNI CCTnn'n_nnAGG
Cuts at: 451 451
Size: 4828
EcoO109I rG'GnC_Cy
Cuts at: 332 552 1965 332
Size: 220 1413 3195
Fragments arranged by size:
3195 1413 220
EcoRI G'AATT_C
Cuts at: 1568 1652 1830 1568
Size: 84 178 4566
Fragments arranged by size:
4566 178 84
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2845 2845
Size: 4828
FspI TGC'GCA
Cuts at: 2607 2607
Size: 4828
HaeII r_GCGC'y
Cuts at: 309 2260 2508 3532 4511 309
Size: 1951 248 1024 979 626
Fragments arranged by size:
1951 1024 979 626 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 536 568 585 617 973 1005 1629 1661 2668
Size: 32 17 32 356 32 624 32 1007
Cuts at: 2668 2698 2700 2730 536
Size: 30 2 30 2634
Fragments arranged by size:
2634 1007 624 356 32 32 32 32
30 30 17 2
HincII GTy'rAC
Cuts at: 956 1865 956
Size: 909 3919
HindIII A'AGCT_T
Cuts at: 1194 1613 1194
Size: 419 4409
KasI G'GCGC_C
Cuts at: 2256 2504 2256
Size: 248 4580
KpnI G_GTAC'C
Cuts at: 1846 1846
Size: 4828
MluI A'CGCG_T
Cuts at: 4261 4261
Size: 4828
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1235 1403 3499 3683 1235
Size: 168 2096 184 2380
Fragments arranged by size:
2380 2096 184 168
MscI TGG'CCA
Cuts at: 2587 2587
Size: 4828
MslI CAynn'nnrTG
Cuts at: 823 1259 2815 2942 823
Size: 436 1556 127 2709
Fragments arranged by size:
2709 1556 436 127
MspA1I CmG'CkG
Cuts at: 787 1937 2251 2611 3626 3871 787
Size: 1150 314 360 1015 245 1744
Fragments arranged by size:
1744 1150 1015 360 314 245
NaeI GCC'GGC
Cuts at: 3007 3007
Size: 4828
NarI GG'CG_CC
Cuts at: 2257 2505 2257
Size: 248 4580
NgoMIV G'CCGG_C
Cuts at: 3005 3005
Size: 4828
NruI TCG'CGA
Cuts at: 3189 3189
Size: 4828
NsiI A_TGCA'T
Cuts at: 1788 1788
Size: 4828
NspI r_CATG'y
Cuts at: 237 1879 2379 3288 237
Size: 1642 500 909 1777
Fragments arranged by size:
1777 1642 909 500
PasI CC'CwG_GG
Cuts at: 921 921
Size: 4828
PciI A'CATG_T
Cuts at: 233 3284 233
Size: 3051 1777
PflMI CCAn_nnn'nTGG
Cuts at: 765 801 765
Size: 36 4792
PfoI T'CCnGG_A
Cuts at: 410 410
Size: 4828
PmeI GTTT'AAAC
Cuts at: 1825 1825
Size: 4828
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1236 1268 3967 3999 1236
Size: 32 2699 32 2065
Fragments arranged by size:
2699 2065 32 32
PpuMI rG'GwC_Cy
Cuts at: 552 552
Size: 4828
PspOMI G'GGCC_C
Cuts at: 331 331
Size: 4828
PspXI vC'TCGA_Gb
Cuts at: 624 624
Size: 4828
PstI C_TGCA'G
Cuts at: 476 1873 2558 476
Size: 1397 685 2746
Fragments arranged by size:
2746 1397 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4828
PvuII CAG'CTG
Cuts at: 2251 2611 2251
Size: 360 4468
RsrII CG'GwC_CG
Cuts at: 3021 3021
Size: 4828
SacI G_AGCT'C
Cuts at: 461 708 1210 1546 1840 461
Size: 247 502 336 294 3449
Fragments arranged by size:
3449 502 336 294 247
SalI G'TCGA_C
Cuts at: 1863 1863
Size: 4828
SapI GCTCTTCn'nnn_
Cuts at: 713 2849 3059 713
Size: 2136 210 2482
Fragments arranged by size:
2482 2136 210
SbfI CC_TGCA'GG
Cuts at: 1873 1873
Size: 4828
ScaI AGT'ACT
Cuts at: 4345 4345
Size: 4828
SfcI C'TryA_G
Cuts at: 33 256 472 1124 1869 2554 3549 3740 33
Size: 223 216 652 745 685 995 191 1121
Fragments arranged by size:
1121 995 745 685 652 223 216 191
SfoI GGC'GCC
Cuts at: 2258 2506 2258
Size: 248 4580
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4828
SmaI CCC'GGG
Cuts at: 1848 1848
Size: 4828
SmlI C'TyrA_G
Cuts at: 624 1661 1981 2896 3390 3652 624
Size: 1037 320 915 494 262 1800
Fragments arranged by size:
1800 1037 915 494 320 262
SnaBI TAC'GTA
Cuts at: 1818 1818
Size: 4828
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4828
SphI G_CATG'C
Cuts at: 1879 2379 1879
Size: 500 4328
StuI AGG'CCT
Cuts at: 1447 1531 1699 1447
Size: 84 168 4576
Fragments arranged by size:
4576 168 84
StyI C'CwwG_G
Cuts at: 398 452 1165 1960 2314 398
Size: 54 713 795 354 2912
Fragments arranged by size:
2912 795 713 354 54
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 667 2409 2542 3001 3212 667
Size: 1742 133 459 211 2283
Fragments arranged by size:
2283 1742 459 211 133
TatI w'GTAC_w
Cuts at: 1763 4343 1763
Size: 2580 2248
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 255 287 4345 4377 255
Size: 32 4058 32 706
Fragments arranged by size:
4058 706 32 32
Tth111I GACn'n_nGTC
Cuts at: 2623 2623
Size: 4828
XbaI T'CTAG_A
Cuts at: 516 1857 516
Size: 1341 3487
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 325 1024 2321 325
Size: 699 1297 2832
Fragments arranged by size:
2832 1297 699
XhoI C'TCGA_G
Cuts at: 624 1661 624
Size: 1037 3791
XmaI C'CCGG_G
Cuts at: 1846 1846
Size: 4828
XmnI GAAnn'nnTTC
Cuts at: 1232 1363 1531 1232
Size: 131 168 4529
Fragments arranged by size:
4529 168 131
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AleI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BfrBI BglII
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI Bsu36I BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NruI NsiI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PspOMI
PspXI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SnaBI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AlfI AloI AscI AvrII
BaeI BbvCI BcgI BglI BmgBI BmtI Bpu10I BsiWI
BsmI BsrGI BssHII BstEII BstXI BstZ17I ClaI CspCI
EcoRV FseI FspAI HpaI MfeI NcoI NdeI NheI
NotI PacI PmlI PshAI PsiI PsrI SacII SanDI
SexAI SfiI SgrAI SrfI SspI SwaI ZraI