Restriction map for
pF1KSDF0041
(Circular) (Six-base) MAPSORT of: pF1KSDF0041.seq Check: 5939 from: 1 to: 4072
pF1KSDF0041 4072 bp
With 182 enzymes: SgfI *
June 20, 2008 10:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 292 428 615 882 292
Size: 136 187 267 3482
Fragments arranged by size:
3482 267 187 136
AatII G_ACGT'C
Cuts at: 1007 1007
Size: 4072
AccI GT'mk_AC
Cuts at: 770 1108 770
Size: 338 3734
Acc65I G'GTAC_C
Cuts at: 341 1086 341
Size: 745 3327
AclI AA'CG_TT
Cuts at: 3912 3912
Size: 4072
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1895 2327 3076 3344 1895
Size: 432 749 268 2623
Fragments arranged by size:
2623 749 432 268
AflIII A'CryG_T
Cuts at: 1422 2528 3505 1422
Size: 1106 977 1989
Fragments arranged by size:
1989 1106 977
AgeI A'CCGG_T
Cuts at: 2460 2460
Size: 4072
AlwNI CAG_nnn'CTG
Cuts at: 813 1375 2944 813
Size: 562 1569 1941
Fragments arranged by size:
1941 1569 562
ApaI G_GGCC'C
Cuts at: 301 301
Size: 4072
ApaLI G'TGCA_C
Cuts at: 2842 2842
Size: 4072
ApoI r'AATT_y
Cuts at: 1074 3425 3681 1074
Size: 2351 256 1465
Fragments arranged by size:
2351 1465 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4072
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4072
AvaI C'yCGr_G
Cuts at: 259 351 889 1090 259
Size: 92 538 201 3241
Fragments arranged by size:
3241 538 201 92
AvrII C'CTAG_G
Cuts at: 204 204
Size: 4072
BamHI G'GATC_C
Cuts at: 169 1095 169
Size: 926 3146
BanI G'GyrC_C
Cuts at: 341 581 610 721 905 1086 1500 1748 1783
Size: 240 29 111 184 181 414 248 35
Cuts at: 1783 341
Size: 2630
Fragments arranged by size:
2630 414 248 240 184 181 111 35
29
BanII G_rGCy'C
Cuts at: 301 971 1084 2114 301
Size: 670 113 1030 2259
Fragments arranged by size:
2259 1030 670 113
BbeI G_GCGC'C
Cuts at: 1504 1752 1504
Size: 248 3824
BbsI GAAGACnn'nnnn_
Cuts at: 999 999
Size: 4072
BciVI GTATCCnnnnn_n'
Cuts at: 878 1968 2731 4072 878
Size: 1090 763 1341 878
Fragments arranged by size:
1341 1090 878 763
BclI T'GATC_A
Cuts at: 1590 1590
Size: 4072
BglI GCCn_nnn'nGGC
Cuts at: 148 148
Size: 4072
BglII A'GATC_T
Cuts at: 1585 3624 1585
Size: 2039 2033
BlpI GC'TnA_GC
Cuts at: 467 1182 467
Size: 715 3357
Bme1580I G_kGCm'C
Cuts at: 301 317 807 1695 1788 2846 301
Size: 16 490 888 93 1058 1527
Fragments arranged by size:
1527 1058 888 490 93 16
BmgBI CAC'GTC
Cuts at: 338 338
Size: 4072
BmrI ACTGGGnnnn_n'
Cuts at: 1282 1363 1698 3844 1282
Size: 81 335 2146 1510
Fragments arranged by size:
2146 1510 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 507 2437 507
Size: 1930 2142
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 166 502 974 166
Size: 336 472 3264
Fragments arranged by size:
3264 472 336
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1246 2125 2619 2917 1246
Size: 879 494 298 2401
Fragments arranged by size:
2401 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 3776 3776
Size: 4072
BsaAI yAC'GTr
Cuts at: 1062 1423 1062
Size: 361 3711
BsaBI GATnn'nnATC
Cuts at: 6 1584 3623 6
Size: 1578 2039 455
Fragments arranged by size:
2039 1578 455
BsaHI Gr'CG_yC
Cuts at: 131 1004 1501 1749 2453 3949 131
Size: 873 497 248 704 1496 254
Fragments arranged by size:
1496 873 704 497 254 248
BsaWI w'CCGG_w
Cuts at: 210 1259 1780 2460 2734 2881 3292 210
Size: 1049 521 680 274 147 411 990
Fragments arranged by size:
1049 990 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 658 688 658
Size: 30 4042
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 666 977 666
Size: 311 3761
BseYI C'CCAG_C
Cuts at: 164 926 970 1495 2253 2832 3328 164
Size: 762 44 525 758 579 496 908
Fragments arranged by size:
908 762 758 579 525 496 44
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 385 406 649 385
Size: 21 243 3808
Fragments arranged by size:
3808 243 21
BsiEI CG_ry'CG
Cuts at: 104 1612 1658 2868 104
Size: 1508 46 1210 1308
Fragments arranged by size:
1508 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 599 1084 1862 2052 2846 599
Size: 485 778 190 794 1825
Fragments arranged by size:
1825 794 778 485 190
BsmBI CGTCTCn'nnnn_
Cuts at: 995 995
Size: 4072
Bsp1286I G_dGCh'C
Cuts at: 301 317 599 807 971 1084 1695 1788 1862
Size: 16 282 208 164 113 611 93 74
Cuts at: 1862 2052 2114 2846 301
Size: 190 62 732 1527
Fragments arranged by size:
1527 732 611 282 208 190 164 113
93 74 62 16
BspEI T'CCGG_A
Cuts at: 3292 3292
Size: 4072
BspHI T'CATG_A
Cuts at: 3248 3248
Size: 4072
BspMI ACCTGCnnnn'nnnn_
Cuts at: 292 379 428 615 882 1120 1334 1636 2017
Size: 87 49 187 267 238 214 302 381
Cuts at: 2017 292
Size: 2347
Fragments arranged by size:
2347 381 302 267 238 214 187 87
49
BsrBI CCG'CTC
Cuts at: 50 2362 2416 3902 4067 50
Size: 2312 54 1486 165 55
Fragments arranged by size:
2312 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1982 1982
Size: 4072
BsrFI r'CCGG_y
Cuts at: 151 383 2068 2249 2460 151
Size: 232 1685 181 211 1763
Fragments arranged by size:
1763 1685 232 211 181
BssSI C'ACGA_G
Cuts at: 2341 2701 2341
Size: 360 3712
BstAPI GCAn_nnn'nTGC
Cuts at: 1447 1447
Size: 4072
BstBI TT'CG_AA
Cuts at: 110 110
Size: 4072
BstXI CCAn_nnnn'nTGG
Cuts at: 144 660 144
Size: 516 3556
BstYI r'GATC_y
Cuts at: 1 169 1095 1562 1585 1920 2166 3180 3266
Size: 168 926 467 23 335 246 1014 86
Cuts at: 3266 3278 3624 1
Size: 12 346 449
Fragments arranged by size:
1014 926 467 449 346 335 246 168
86 23 12
BtgI C'CryG_G
Cuts at: 125 137 426 474 501 606 614 125
Size: 12 289 48 27 105 8 3583
Fragments arranged by size:
3583 289 105 48 27 12 8
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2020 2201 2364 2020
Size: 181 163 3728
Fragments arranged by size:
3728 181 163
BtsI GCAGTG_nn'
Cuts at: 1313 1313
Size: 4072
DraI TTT'AAA
Cuts at: 1069 1069
Size: 4072
DraIII CAC_nnn'GTG
Cuts at: 321 321
Size: 4072
DrdI GACnn_nn'nnGTC
Cuts at: 1776 2636 1776
Size: 860 3212
EaeI y'GGCC_r
Cuts at: 1655 1829 2220 2247 3594 1655
Size: 174 391 27 1347 2133
Fragments arranged by size:
2133 1347 391 174 27
EagI C'GGCC_G
Cuts at: 1655 1655
Size: 4072
EarI CTCTTCn'nnn_
Cuts at: 184 193 2093 2303 184
Size: 9 1900 210 1953
Fragments arranged by size:
1953 1900 210 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2278 2590 2736 3882 2278
Size: 312 146 1146 2468
Fragments arranged by size:
2468 1146 312 146
EcoICRI GAG'CTC
Cuts at: 1082 1082
Size: 4072
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 166 502 974 1895 2327 3076 3344 166
Size: 336 472 921 432 749 268 894
Fragments arranged by size:
921 894 749 472 432 336 268
EcoO109I rG'GnC_Cy
Cuts at: 298 411 994 1209 298
Size: 113 583 215 3161
Fragments arranged by size:
3161 583 215 113
EcoRI G'AATT_C
Cuts at: 1074 1074
Size: 4072
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2089 2089
Size: 4072
FspI TGC'GCA
Cuts at: 526 1851 526
Size: 1325 2747
HaeII r_GCGC'y
Cuts at: 165 384 474 1504 1752 2776 3755 165
Size: 219 90 1030 248 1024 979 482
Fragments arranged by size:
1030 1024 979 482 248 219 90
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 161 193 634 666 1912 1942 1944 1974 161
Size: 32 441 32 1246 30 2 30 2259
Fragments arranged by size:
2259 1246 441 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1109 1109
Size: 4072
KasI G'GCGC_C
Cuts at: 1500 1748 1500
Size: 248 3824
KpnI G_GTAC'C
Cuts at: 345 1090 345
Size: 745 3327
MluI A'CGCG_T
Cuts at: 3505 3505
Size: 4072
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 518 677 818 2743 2927 518
Size: 159 141 1925 184 1663
Fragments arranged by size:
1925 1663 184 159 141
MscI TGG'CCA
Cuts at: 1831 1831
Size: 4072
MslI CAynn'nnrTG
Cuts at: 520 2059 2186 520
Size: 1539 127 2406
Fragments arranged by size:
2406 1539 127
MspA1I CmG'CkG
Cuts at: 158 930 1181 1495 1855 2870 3115 158
Size: 772 251 314 360 1015 245 1115
Fragments arranged by size:
1115 1015 772 360 314 251 245
NaeI GCC'GGC
Cuts at: 153 385 2251 153
Size: 232 1866 1974
Fragments arranged by size:
1974 1866 232
NarI GG'CG_CC
Cuts at: 1501 1749 1501
Size: 248 3824
NcoI C'CATG_G
Cuts at: 125 426 501 606 614 125
Size: 301 75 105 8 3583
Fragments arranged by size:
3583 301 105 75 8
NgoMIV G'CCGG_C
Cuts at: 151 383 2249 151
Size: 232 1866 1974
Fragments arranged by size:
1974 1866 232
NruI TCG'CGA
Cuts at: 2433 2433
Size: 4072
NspI r_CATG'y
Cuts at: 1123 1623 2532 1123
Size: 500 909 2663
Fragments arranged by size:
2663 909 500
PasI CC'CwG_GG
Cuts at: 879 879
Size: 4072
PciI A'CATG_T
Cuts at: 2528 2528
Size: 4072
PflMI CCAn_nnn'nTGG
Cuts at: 462 480 813 462
Size: 18 333 3721
Fragments arranged by size:
3721 333 18
PfoI T'CCnGG_A
Cuts at: 407 407
Size: 4072
PmeI GTTT'AAAC
Cuts at: 1069 1069
Size: 4072
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3211 3243 3211
Size: 32 4040
PpuMI rG'GwC_Cy
Cuts at: 411 411
Size: 4072
PspOMI G'GGCC_C
Cuts at: 297 297
Size: 4072
PspXI vC'TCGA_Gb
Cuts at: 351 351
Size: 4072
PstI C_TGCA'G
Cuts at: 289 626 708 1117 1802 289
Size: 337 82 409 685 2559
Fragments arranged by size:
2559 685 409 337 82
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4072
PvuII CAG'CTG
Cuts at: 158 1495 1855 158
Size: 1337 360 2375
Fragments arranged by size:
2375 1337 360
RsrII CG'GwC_CG
Cuts at: 2265 2265
Size: 4072
SacI G_AGCT'C
Cuts at: 1084 1084
Size: 4072
SalI G'TCGA_C
Cuts at: 1107 1107
Size: 4072
SapI GCTCTTCn'nnn_
Cuts at: 184 2093 2303 184
Size: 1909 210 1953
Fragments arranged by size:
1953 1909 210
SbfI CC_TGCA'GG
Cuts at: 289 1117 289
Size: 828 3244
ScaI AGT'ACT
Cuts at: 3589 3589
Size: 4072
SfcI C'TryA_G
Cuts at: 33 285 622 704 1113 1798 2793 2984 33
Size: 252 337 82 409 685 995 191 1121
Fragments arranged by size:
1121 995 685 409 337 252 191 82
SfiI GGCCn_nnn'nGGCC
Cuts at: 148 148
Size: 4072
SfoI GGC'GCC
Cuts at: 1502 1750 1502
Size: 248 3824
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4072
SmaI CCC'GGG
Cuts at: 1092 1092
Size: 4072
SmlI C'TyrA_G
Cuts at: 351 1225 2140 2634 2896 351
Size: 874 915 494 262 1527
Fragments arranged by size:
1527 915 874 494 262
SnaBI TAC'GTA
Cuts at: 1062 1062
Size: 4072
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4072
SphI G_CATG'C
Cuts at: 1123 1623 1123
Size: 500 3572
StyI C'CwwG_G
Cuts at: 125 204 426 501 606 614 1204 1558 125
Size: 79 222 75 105 8 590 354 2639
Fragments arranged by size:
2639 590 354 222 105 79 75 8
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1653 1786 2245 2456 1653
Size: 133 459 211 3269
Fragments arranged by size:
3269 459 211 133
TatI w'GTAC_w
Cuts at: 3587 3587
Size: 4072
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 898 930 3589 3621 898
Size: 32 2659 32 1349
Fragments arranged by size:
2659 1349 32 32
Tth111I GACn'n_nGTC
Cuts at: 1027 1867 1027
Size: 840 3232
XbaI T'CTAG_A
Cuts at: 1101 1101
Size: 4072
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 459 1565 459
Size: 1106 2966
XhoI C'TCGA_G
Cuts at: 351 351
Size: 4072
XmaI C'CCGG_G
Cuts at: 1090 1090
Size: 4072
ZraI GAC'GTC
Cuts at: 1005 1005
Size: 4072
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII
BamHI BanI BanII BbeI BbsI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NruI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PspOMI
PspXI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfiI SfoI SgfI SmaI SmlI
SnaBI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AleI AlfI AloI AscI BaeI
BbvCI BcgI BfrBI BmtI Bpu10I BsiWI BsmI BsrGI
BssHII BstEII BstZ17I Bsu36I ClaI CspCI EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NsiI PacI PmlI PshAI PsiI PsrI SacII SanDI
SexAI SgrAI SrfI SspI StuI SwaI XmnI