Restriction map for
pF1KSDB3800
(Circular) (Six-base) MAPSORT of: pF1KSDB3800.seq Check: 8676 from: 1 to: 7106
pF1KSDB3800 7106 bp
With 182 enzymes: SgfI *
June 20, 2008 10:36 ..
AccI GT'mk_AC
Cuts at: 3172 4094 4144 3172
Size: 922 50 6134
Fragments arranged by size:
6134 922 50
Acc65I G'GTAC_C
Cuts at: 1908 2238 4122 1908
Size: 330 1884 4892
Fragments arranged by size:
4892 1884 330
AclI AA'CG_TT
Cuts at: 3641 6946 3641
Size: 3305 3801
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 827 2540 2862 3141 3901 4931 5363 6110 6378
Size: 1713 322 279 760 1030 432 747 268
Cuts at: 6378 827
Size: 1555
Fragments arranged by size:
1713 1555 1030 760 747 432 322 279
268
AflIII A'CryG_T
Cuts at: 3912 4458 5562 6539 3912
Size: 546 1104 977 4479
Fragments arranged by size:
4479 1104 977 546
AgeI A'CCGG_T
Cuts at: 5494 5494
Size: 7106
AleI CACnn'nnGTG
Cuts at: 758 758
Size: 7106
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3511 3543 3511
Size: 32 7074
AlwNI CAG_nnn'CTG
Cuts at: 539 2946 3543 4411 5978 539
Size: 2407 597 868 1567 1667
Fragments arranged by size:
2407 1667 1567 868 597
ApaI G_GGCC'C
Cuts at: 4051 4051
Size: 7106
ApaLI G'TGCA_C
Cuts at: 5876 5876
Size: 7106
ApoI r'AATT_y
Cuts at: 441 741 973 1589 1856 2658 3233 4110 6459
Size: 300 232 616 267 802 575 877 2349
Cuts at: 6459 6715 441
Size: 256 832
Fragments arranged by size:
2349 877 832 802 616 575 300 267
256 232
AseI AT'TA_AT
Cuts at: 20 20
Size: 7106
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7106
AvaI C'yCGr_G
Cuts at: 4126 4126
Size: 7106
AvrII C'CTAG_G
Cuts at: 718 718
Size: 7106
BamHI G'GATC_C
Cuts at: 459 4131 459
Size: 3672 3434
BanI G'GyrC_C
Cuts at: 1908 2238 3304 3415 4008 4122 4536 4784 4819
Size: 330 1066 111 593 114 414 248 35
Cuts at: 4819 1908
Size: 4195
Fragments arranged by size:
4195 1066 593 414 330 248 114 111
35
BanII G_rGCy'C
Cuts at: 2140 2211 2218 4051 4120 5150 2140
Size: 71 7 1833 69 1030 4096
Fragments arranged by size:
4096 1833 1030 71 69 7
BbeI G_GCGC'C
Cuts at: 4540 4788 4540
Size: 248 6858
BbsI GAAGACnn'nnnn_
Cuts at: 161 161
Size: 7106
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2809 2843 3636 3670 2809
Size: 34 793 34 6245
Fragments arranged by size:
6245 793 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3454 5004 5765 7106 3454
Size: 1550 761 1341 3454
Fragments arranged by size:
3454 1550 1341 761
BclI T'GATC_A
Cuts at: 189 396 4626 189
Size: 207 4230 2669
Fragments arranged by size:
4230 2669 207
BfrBI ATG'CAT
Cuts at: 282 2730 282
Size: 2448 4658
BglII A'GATC_T
Cuts at: 490 726 1784 4621 6658 490
Size: 236 1058 2837 2037 938
Fragments arranged by size:
2837 2037 1058 938 236
BlpI GC'TnA_GC
Cuts at: 2279 4218 2279
Size: 1939 5167
Bme1580I G_kGCm'C
Cuts at: 4051 4731 4824 5880 4051
Size: 680 93 1056 5277
Fragments arranged by size:
5277 1056 680 93
BmrI ACTGGGnnnn_n'
Cuts at: 2401 3913 4318 4399 4734 6878 2401
Size: 1512 405 81 335 2144 2629
Fragments arranged by size:
2629 2144 1512 405 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1014 2492 5473 1014
Size: 1478 2981 2647
Fragments arranged by size:
2981 2647 1478
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1799 1883 2285 1799
Size: 84 402 6620
Fragments arranged by size:
6620 402 84
Bpu10I CC'TnA_GC
Cuts at: 2195 2195
Size: 7106
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2084 2112 3552 4282 5161 5653 5951 2084
Size: 28 1440 730 879 492 298 3239
Fragments arranged by size:
3239 1440 879 730 492 298 28
BsaI GGTCTCn'nnnn_
Cuts at: 575 6810 575
Size: 6235 871
BsaAI yAC'GTr
Cuts at: 3913 4098 4459 3913
Size: 185 361 6560
Fragments arranged by size:
6560 361 185
BsaBI GATnn'nnATC
Cuts at: 6 1832 2992 4620 6657 6
Size: 1826 1160 1628 2037 455
Fragments arranged by size:
2037 1826 1628 1160 455
BsaHI Gr'CG_yC
Cuts at: 4537 4785 5487 6983 4537
Size: 248 702 1496 4660
Fragments arranged by size:
4660 1496 702 248
BsaWI w'CCGG_w
Cuts at: 818 1911 2775 4295 4816 5494 5768 5915 6326
Size: 1093 864 1520 521 678 274 147 411
Cuts at: 6326 818
Size: 1598
Fragments arranged by size:
1598 1520 1093 864 678 521 411 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2163 2193 2258 2288 2163
Size: 30 65 30 6981
Fragments arranged by size:
6981 65 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 808 808
Size: 7106
BseYI C'CCAG_C
Cuts at: 3238 4531 5289 5866 6362 3238
Size: 1293 758 577 496 3982
Fragments arranged by size:
3982 1293 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2839 2839
Size: 7106
BsiEI CG_ry'CG
Cuts at: 104 4648 4694 5902 104
Size: 4544 46 1208 1308
Fragments arranged by size:
4544 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 802 2211 2218 3608 4120 4898 5088 5880 802
Size: 1409 7 1390 512 778 190 792 2028
Fragments arranged by size:
2028 1409 1390 792 778 512 190 7
BsmI GAATG_Cn'
Cuts at: 1271 1366 2100 2732 1271
Size: 95 734 632 5645
Fragments arranged by size:
5645 734 632 95
BsmBI CGTCTCn'nnnn_
Cuts at: 2437 3115 2437
Size: 678 6428
Bsp1286I G_dGCh'C
Cuts at: 802 2140 2211 2218 3608 4051 4120 4731 4824
Size: 1338 71 7 1390 443 69 611 93
Cuts at: 4824 4898 5088 5150 5880 802
Size: 74 190 62 730 2028
Fragments arranged by size:
2028 1390 1338 730 611 443 190 93
74 71 69 62 7
BspEI T'CCGG_A
Cuts at: 6326 6326
Size: 7106
BspHI T'CATG_A
Cuts at: 3137 6282 3137
Size: 3145 3961
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1144 4156 4370 4672 5053 1144
Size: 3012 214 302 381 3197
Fragments arranged by size:
3197 3012 381 302 214
BsrBI CCG'CTC
Cuts at: 50 5398 5452 6936 7101 50
Size: 5348 54 1484 165 55
Fragments arranged by size:
5348 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2510 3963 5018 2510
Size: 1453 1055 4598
Fragments arranged by size:
4598 1453 1055
BsrFI r'CCGG_y
Cuts at: 4011 5104 5285 5494 4011
Size: 1093 181 209 5623
Fragments arranged by size:
5623 1093 209 181
BsrGI T'GTAC_A
Cuts at: 3345 3345
Size: 7106
BssSI C'ACGA_G
Cuts at: 5377 5735 5377
Size: 358 6748
BstAPI GCAn_nnn'nTGC
Cuts at: 3304 3543 4483 3304
Size: 239 940 5927
Fragments arranged by size:
5927 940 239
BstBI TT'CG_AA
Cuts at: 110 5467 110
Size: 5357 1749
BstEII G'GTnAC_C
Cuts at: 1435 1435
Size: 7106
BstXI CCAn_nnnn'nTGG
Cuts at: 2235 3483 2235
Size: 1248 5858
BstYI r'GATC_y
Cuts at: 1 459 490 726 1784 2318 4131 4598 4621
Size: 458 31 236 1058 534 1813 467 23
Cuts at: 4621 4956 5202 6214 6300 6312 6658 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1813 1058 1012 534 467 458 449 346
335 246 236 86 31 23 12
BstZ17I GTA'TAC
Cuts at: 3173 4095 3173
Size: 922 6184
Bsu36I CC'TnA_GG
Cuts at: 1103 2202 1103
Size: 1099 6007
BtgI C'CryG_G
Cuts at: 3680 3711 4020 3680
Size: 31 309 6766
Fragments arranged by size:
6766 309 31
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 5056 5237 5400 5056
Size: 181 163 6762
Fragments arranged by size:
6762 181 163
BtsI GCAGTG_nn'
Cuts at: 204 1977 2741 2944 3541 4349 204
Size: 1773 764 203 597 808 2961
Fragments arranged by size:
2961 1773 808 764 597 203
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2960 2995 2960
Size: 35 7071
DraI TTT'AAA
Cuts at: 1166 1424 2255 2651 4105 1166
Size: 258 831 396 1454 4167
Fragments arranged by size:
4167 1454 831 396 258
DraIII CAC_nnn'GTG
Cuts at: 2817 2817
Size: 7106
DrdI GACnn_nn'nnGTC
Cuts at: 4812 5670 4812
Size: 858 6248
EaeI y'GGCC_r
Cuts at: 3960 4691 4865 5256 5283 6628 3960
Size: 731 174 391 27 1345 4438
Fragments arranged by size:
4438 1345 731 391 174 27
EagI C'GGCC_G
Cuts at: 4691 4691
Size: 7106
EarI CTCTTCn'nnn_
Cuts at: 2573 3459 5129 5339 2573
Size: 886 1670 210 4340
Fragments arranged by size:
4340 1670 886 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3598 5314 5624 5770 6916 3598
Size: 1716 310 146 1146 3788
Fragments arranged by size:
3788 1716 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2209 2216 4118 2209
Size: 7 1902 5197
Fragments arranged by size:
5197 1902 7
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 827 1799 1883 2285 2540 2862 3141 3901 4931
Size: 972 84 402 255 322 279 760 1030
Cuts at: 4931 5363 6110 6378 827
Size: 432 747 268 1555
Fragments arranged by size:
1555 1030 972 760 747 432 402 322
279 268 255 84
EcoO109I rG'GnC_Cy
Cuts at: 693 4028 4047 4048 4245 693
Size: 3335 19 1 197 3554
Fragments arranged by size:
3554 3335 197 19 1
EcoRI G'AATT_C
Cuts at: 441 973 4110 441
Size: 532 3137 3437
Fragments arranged by size:
3437 3137 532
EcoRV GAT'ATC
Cuts at: 2802 2802
Size: 7106
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 976 2545 2820 5125 976
Size: 1569 275 2305 2957
Fragments arranged by size:
2957 2305 1569 275
FspI TGC'GCA
Cuts at: 4887 4887
Size: 7106
HaeII r_GCGC'y
Cuts at: 3443 4004 4540 4788 5810 6789 3443
Size: 561 536 248 1022 979 3760
Fragments arranged by size:
3760 1022 979 561 536 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 475 507 2572 2604 3661 3693 4948 4978 4980
Size: 32 2065 32 1057 32 1255 30 2
Cuts at: 4980 5010 475
Size: 30 2571
Fragments arranged by size:
2571 2065 1255 1057 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 498 1031 4145 498
Size: 533 3114 3459
Fragments arranged by size:
3459 3114 533
HindIII A'AGCT_T
Cuts at: 3842 3842
Size: 7106
HpaI GTT'AAC
Cuts at: 498 498
Size: 7106
KasI G'GCGC_C
Cuts at: 4536 4784 4536
Size: 248 6858
KpnI G_GTAC'C
Cuts at: 1912 2242 4126 1912
Size: 330 1884 4892
Fragments arranged by size:
4892 1884 330
MfeI C'AATT_G
Cuts at: 3789 3789
Size: 7106
MluI A'CGCG_T
Cuts at: 6539 6539
Size: 7106
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1119 2463 2475 2594 5777 5961 1119
Size: 1344 12 119 3183 184 2264
Fragments arranged by size:
3183 2264 1344 184 119 12
MscI TGG'CCA
Cuts at: 3962 4867 3962
Size: 905 6201
MslI CAynn'nnrTG
Cuts at: 287 758 2233 5095 5222 287
Size: 471 1475 2862 127 2171
Fragments arranged by size:
2862 2171 1475 471 127
MspA1I CmG'CkG
Cuts at: 4217 4531 4891 5904 6149 4217
Size: 314 360 1013 245 5174
Fragments arranged by size:
5174 1013 360 314 245
NaeI GCC'GGC
Cuts at: 5287 5287
Size: 7106
NarI GG'CG_CC
Cuts at: 4537 4785 4537
Size: 248 6858
NcoI C'CATG_G
Cuts at: 3680 3711 4020 3680
Size: 31 309 6766
Fragments arranged by size:
6766 309 31
NgoMIV G'CCGG_C
Cuts at: 5285 5285
Size: 7106
NsiI A_TGCA'T
Cuts at: 284 2732 284
Size: 2448 4658
NspI r_CATG'y
Cuts at: 855 1991 2734 3292 3835 4159 4659 5566 855
Size: 1136 743 558 543 324 500 907 2395
Fragments arranged by size:
2395 1136 907 743 558 543 500 324
PacI TTA_AT'TAA
Cuts at: 167 167
Size: 7106
PasI CC'CwG_GG
Cuts at: 636 636
Size: 7106
PciI A'CATG_T
Cuts at: 5562 5562
Size: 7106
PflMI CCAn_nnn'nTGG
Cuts at: 3115 3624 3115
Size: 509 6597
PmeI GTTT'AAAC
Cuts at: 4105 4105
Size: 7106
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6245 6277 6245
Size: 32 7074
PpuMI rG'GwC_Cy
Cuts at: 693 693
Size: 7106
PsiI TTA'TAA
Cuts at: 335 335
Size: 7106
PspOMI G'GGCC_C
Cuts at: 4047 4047
Size: 7106
PstI C_TGCA'G
Cuts at: 1984 2382 3516 3810 4153 4838 1984
Size: 398 1134 294 343 685 4252
Fragments arranged by size:
4252 1134 685 398 343 294
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7106
PvuII CAG'CTG
Cuts at: 4531 4891 4531
Size: 360 6746
RsrII CG'GwC_CG
Cuts at: 5301 5301
Size: 7106
SacI G_AGCT'C
Cuts at: 2211 2218 4120 2211
Size: 7 1902 5197
Fragments arranged by size:
5197 1902 7
SalI G'TCGA_C
Cuts at: 4143 4143
Size: 7106
SapI GCTCTTCn'nnn_
Cuts at: 5129 5339 5129
Size: 210 6896
SbfI CC_TGCA'GG
Cuts at: 4153 4153
Size: 7106
ScaI AGT'ACT
Cuts at: 2960 6623 2960
Size: 3663 3443
SfcI C'TryA_G
Cuts at: 33 177 968 1087 1470 1980 2016 2378 2764
Size: 144 791 119 383 510 36 362 386
Cuts at: 2764 3512 3665 3806 4149 4834 5827 6018 33
Size: 748 153 141 343 685 993 191 1121
Fragments arranged by size:
1121 993 791 748 685 510 386 383
362 343 191 153 144 141 119 36
SfoI GGC'GCC
Cuts at: 4538 4786 4538
Size: 248 6858
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7106
SmaI CCC'GGG
Cuts at: 4128 4128
Size: 7106
SmlI C'TyrA_G
Cuts at: 2099 2127 3531 4261 5176 5668 5930 2099
Size: 28 1404 730 915 492 262 3275
Fragments arranged by size:
3275 1404 915 730 492 262 28
SnaBI TAC'GTA
Cuts at: 4098 4098
Size: 7106
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7106
SphI G_CATG'C
Cuts at: 2734 4159 4659 2734
Size: 1425 500 5181
Fragments arranged by size:
5181 1425 500
SspI AAT'ATT
Cuts at: 1374 1374
Size: 7106
StuI AGG'CCT
Cuts at: 2200 2200
Size: 7106
StyI C'CwwG_G
Cuts at: 718 1508 2720 2892 3013 3680 3711 4004 4020
Size: 790 1212 172 121 667 31 293 16
Cuts at: 4020 4240 4594 718
Size: 220 354 3230
Fragments arranged by size:
3230 1212 790 667 354 293 220 172
121 31 16
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 489 1876 3612 4689 4822 5281 5490 489
Size: 1387 1736 1077 133 459 209 2105
Fragments arranged by size:
2105 1736 1387 1077 459 209 133
TatI w'GTAC_w
Cuts at: 2958 3345 3980 6621 2958
Size: 387 635 2641 3443
Fragments arranged by size:
3443 2641 635 387
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2258 2290 2770 2802 3899 3931 6623 6655 2258
Size: 32 480 32 1097 32 2692 32 2709
Fragments arranged by size:
2709 2692 1097 480 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2407 4903 2407
Size: 2496 4610
XbaI T'CTAG_A
Cuts at: 1158 4137 1158
Size: 2979 4127
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 643 4601 643
Size: 3958 3148
XmaI C'CCGG_G
Cuts at: 4126 4126
Size: 7106
XmnI GAAnn'nnTTC
Cuts at: 1082 1999 3559 3706 1082
Size: 917 1560 147 4482
Fragments arranged by size:
4482 1560 917 147
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AloI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII
BamHI BanI BanII BbeI BbsI BcgI BciVI BclI
BfrBI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI
BtsI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NsiI NspI PacI PasI PciI PflMI PmeI
PpiI PpuMI PsiI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AscI BaeI
BbvCI BglI BmgBI BmtI BsiWI BssHII ClaI EcoNI
FseI FspAI NdeI NheI NotI NruI PfoI PmlI
PshAI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SrfI SwaI XhoI ZraI