Restriction map for
pF1KSDB3041
(Circular) (Six-base) MAPSORT of: pF1KSDB3041.seq Check: 9448 from: 1 to: 6833
pF1KSDB3041 6833 bp
With 182 enzymes: SgfI *
January 7, 2013 14:39 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3639 3639
Size: 6833
AccI GT'mk_AC
Cuts at: 842 3871 842
Size: 3029 3804
Acc65I G'GTAC_C
Cuts at: 1237 3849 1237
Size: 2612 4221
AclI AA'CG_TT
Cuts at: 6673 6673
Size: 6833
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 902 1855 1981 2555 3034 4658 5090 5837 6105
Size: 953 126 574 479 1624 432 747 268
Cuts at: 6105 902
Size: 1630
Fragments arranged by size:
1630 1624 953 747 574 479 432 268
126
AfeI AGC'GCT
Cuts at: 1721 1721
Size: 6833
AflIII A'CryG_T
Cuts at: 3708 4185 5289 6266 3708
Size: 477 1104 977 4275
Fragments arranged by size:
4275 1104 977 477
AgeI A'CCGG_T
Cuts at: 5221 5221
Size: 6833
AleI CACnn'nnGTG
Cuts at: 3015 3015
Size: 6833
AlwNI CAG_nnn'CTG
Cuts at: 1308 1938 2251 2646 3000 3003 3105 3654 4138
Size: 630 313 395 354 3 102 549 484
Cuts at: 4138 5705 1308
Size: 1567 2436
Fragments arranged by size:
2436 1567 630 549 484 395 354 313
102 3
ApaLI G'TGCA_C
Cuts at: 5603 5603
Size: 6833
ApoI r'AATT_y
Cuts at: 146 523 566 2608 3837 6186 6442 146
Size: 377 43 2042 1229 2349 256 537
Fragments arranged by size:
2349 2042 1229 537 377 256 43
AseI AT'TA_AT
Cuts at: 20 908 20
Size: 888 5945
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6833
AvaI C'yCGr_G
Cuts at: 2348 3853 2348
Size: 1505 5328
AvrII C'CTAG_G
Cuts at: 472 472
Size: 6833
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1583 1616 1583
Size: 33 6800
BamHI G'GATC_C
Cuts at: 621 3555 3858 621
Size: 2934 303 3596
Fragments arranged by size:
3596 2934 303
BanI G'GyrC_C
Cuts at: 1237 3849 4263 4511 4546 1237
Size: 2612 414 248 35 3524
Fragments arranged by size:
3524 2612 414 248 35
BanII G_rGCy'C
Cuts at: 1436 3765 3847 4877 1436
Size: 2329 82 1030 3392
Fragments arranged by size:
3392 2329 1030 82
BbeI G_GCGC'C
Cuts at: 4267 4515 4267
Size: 248 6585
BbsI GAAGACnn'nnnn_
Cuts at: 309 667 1496 1637 2672 3670 309
Size: 358 829 141 1035 998 3472
Fragments arranged by size:
3472 1035 998 829 358 141
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3228 3262 3228
Size: 34 6799
BciVI GTATCCnnnnn_n'
Cuts at: 675 3144 4731 5492 6833 675
Size: 2469 1587 761 1341 675
Fragments arranged by size:
2469 1587 1341 761 675
BclI T'GATC_A
Cuts at: 4353 4353
Size: 6833
BglI GCCn_nnn'nGGC
Cuts at: 733 3491 733
Size: 2758 4075
BglII A'GATC_T
Cuts at: 4348 6385 4348
Size: 2037 4796
BlpI GC'TnA_GC
Cuts at: 2879 3004 3945 2879
Size: 125 941 5767
Fragments arranged by size:
5767 941 125
Bme1580I G_kGCm'C
Cuts at: 233 986 2724 3511 4458 4551 5607 233
Size: 753 1738 787 947 93 1056 1459
Fragments arranged by size:
1738 1459 1056 947 787 753 93
BmrI ACTGGGnnnn_n'
Cuts at: 741 1542 4045 4126 4461 6605 741
Size: 801 2503 81 335 2144 969
Fragments arranged by size:
2503 2144 969 801 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2766 5200 2766
Size: 2434 4399
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1915 1960 2263 3100 3149 3250 3571 3725 1915
Size: 45 303 837 49 101 321 154 5023
Fragments arranged by size:
5023 837 321 303 154 101 49 45
Bpu10I CC'TnA_GC
Cuts at: 1435 3194 1435
Size: 1759 5074
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2251 2435 3055 4009 4888 5380 5678 2251
Size: 184 620 954 879 492 298 3406
Fragments arranged by size:
3406 954 879 620 492 298 184
BsaI GGTCTCn'nnnn_
Cuts at: 2153 2754 6537 2153
Size: 601 3783 2449
Fragments arranged by size:
3783 2449 601
BsaAI yAC'GTr
Cuts at: 3825 4186 3825
Size: 361 6472
BsaBI GATnn'nnATC
Cuts at: 6 4347 6384 6
Size: 4341 2037 455
Fragments arranged by size:
4341 2037 455
BsaHI Gr'CG_yC
Cuts at: 4264 4512 5214 6710 4264
Size: 248 702 1496 4387
Fragments arranged by size:
4387 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2510 4022 4543 5221 5495 5642 6053 2510
Size: 1512 521 678 274 147 411 3290
Fragments arranged by size:
3290 1512 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2732 2762 3095 3125 2732
Size: 30 333 30 6440
Fragments arranged by size:
6440 333 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1330 2254 2742 3103 1330
Size: 924 488 361 5060
Fragments arranged by size:
5060 924 488 361
BseYI C'CCAG_C
Cuts at: 2684 3672 4258 5016 5593 6089 2684
Size: 988 586 758 577 496 3428
Fragments arranged by size:
3428 988 758 586 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 3441 3441
Size: 6833
BsiEI CG_ry'CG
Cuts at: 104 4375 4421 5629 104
Size: 4271 46 1208 1308
Fragments arranged by size:
4271 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 954 1436 2225 2622 3010 3847 4625 4815 5607
Size: 482 789 397 388 837 778 190 792
Cuts at: 5607 954
Size: 2180
Fragments arranged by size:
2180 837 792 789 778 482 397 388
190
BsmI GAATG_Cn'
Cuts at: 3273 3273
Size: 6833
BsmBI CGTCTCn'nnnn_
Cuts at: 1627 1933 2269 1627
Size: 306 336 6191
Fragments arranged by size:
6191 336 306
Bsp1286I G_dGCh'C
Cuts at: 233 954 986 1436 2225 2622 2724 3010 3511
Size: 721 32 450 789 397 102 286 501
Cuts at: 3511 3765 3847 4458 4551 4625 4815 4877 5607
Size: 254 82 611 93 74 190 62 730
Cuts at: 5607 233
Size: 1459
Fragments arranged by size:
1459 789 730 721 611 501 450 397
286 254 190 102 93 82 74 62
32
BspEI T'CCGG_A
Cuts at: 2510 6053 2510
Size: 3543 3290
BspHI T'CATG_A
Cuts at: 6009 6009
Size: 6833
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1538 1631 3639 3883 4097 4399 4780 1538
Size: 93 2008 244 214 302 381 3591
Fragments arranged by size:
3591 2008 381 302 244 214 93
BsrBI CCG'CTC
Cuts at: 50 5125 5179 6663 6828 50
Size: 5075 54 1484 165 55
Fragments arranged by size:
5075 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 4745 4745
Size: 6833
BsrFI r'CCGG_y
Cuts at: 4831 5012 5221 4831
Size: 181 209 6443
Fragments arranged by size:
6443 209 181
BssSI C'ACGA_G
Cuts at: 590 3209 5104 5462 590
Size: 2619 1895 358 1961
Fragments arranged by size:
2619 1961 1895 358
BstAPI GCAn_nnn'nTGC
Cuts at: 433 2251 2646 2826 4210 433
Size: 1818 395 180 1384 3056
Fragments arranged by size:
3056 1818 1384 395 180
BstBI TT'CG_AA
Cuts at: 110 5194 110
Size: 5084 1749
BstEII G'GTnAC_C
Cuts at: 3567 3567
Size: 6833
BstXI CCAn_nnnn'nTGG
Cuts at: 2706 2706
Size: 6833
BstYI r'GATC_y
Cuts at: 1 573 621 1249 1461 2441 3252 3555 3620
Size: 572 48 628 212 980 811 303 65
Cuts at: 3620 3858 4325 4348 4683 4929 5941 6027 6039
Size: 238 467 23 335 246 1012 86 12
Cuts at: 6039 6385 1
Size: 346 449
Fragments arranged by size:
1012 980 811 628 572 467 449 346
335 303 246 238 212 86 65 48
23 12
Bsu36I CC'TnA_GG
Cuts at: 242 626 3146 242
Size: 384 2520 3929
Fragments arranged by size:
3929 2520 384
BtgI C'CryG_G
Cuts at: 731 1230 2325 731
Size: 499 1095 5239
Fragments arranged by size:
5239 1095 499
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3653 4783 4964 5127 3653
Size: 1130 181 163 5359
Fragments arranged by size:
5359 1130 181 163
BtsI GCAGTG_nn'
Cuts at: 2339 3418 4076 2339
Size: 1079 658 5096
Fragments arranged by size:
5096 1079 658
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 954 989 3396 3431 954
Size: 35 2407 35 4356
Fragments arranged by size:
4356 2407 35 35
DraI TTT'AAA
Cuts at: 357 3832 357
Size: 3475 3358
DraIII CAC_nnn'GTG
Cuts at: 3473 3473
Size: 6833
DrdI GACnn_nn'nnGTC
Cuts at: 1641 4539 5397 1641
Size: 2898 858 3077
Fragments arranged by size:
3077 2898 858
EaeI y'GGCC_r
Cuts at: 3767 4418 4592 4983 5010 6355 3767
Size: 651 174 391 27 1345 4245
Fragments arranged by size:
4245 1345 651 391 174 27
EagI C'GGCC_G
Cuts at: 4418 4418
Size: 6833
EarI CTCTTCn'nnn_
Cuts at: 128 496 1424 3177 3806 4856 5066 128
Size: 368 928 1753 629 1050 210 1895
Fragments arranged by size:
1895 1753 1050 928 629 368 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 5041 5351 5497 6643 5041
Size: 310 146 1146 5231
Fragments arranged by size:
5231 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1434 3845 1434
Size: 2411 4422
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 902 1855 1915 1960 1981 2263 2555 3034 3100
Size: 953 60 45 21 282 292 479 66
Cuts at: 3100 3149 3250 3571 3725 4658 5090 5837 6105
Size: 49 101 321 154 933 432 747 268
Cuts at: 6105 902
Size: 1630
Fragments arranged by size:
1630 953 933 747 479 432 321 292
282 268 154 101 66 60 49 45
21
EcoNI CCTnn'n_nnAGG
Cuts at: 629 1312 3115 629
Size: 683 1803 4347
Fragments arranged by size:
4347 1803 683
EcoO109I rG'GnC_Cy
Cuts at: 1737 3972 1737
Size: 2235 4598
EcoRI G'AATT_C
Cuts at: 146 2608 3837 146
Size: 2462 1229 3142
Fragments arranged by size:
3142 2462 1229
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 534 993 3519 4852 534
Size: 459 2526 1333 2515
Fragments arranged by size:
2526 2515 1333 459
FspI TGC'GCA
Cuts at: 4614 4614
Size: 6833
HaeII r_GCGC'y
Cuts at: 166 337 1723 1917 4267 4515 5537 6516 166
Size: 171 1386 194 2350 248 1022 979 483
Fragments arranged by size:
2350 1386 1022 979 483 248 194 171
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2433 2465 2732 2764 4675 4705 4707 4737 2433
Size: 32 267 32 1911 30 2 30 4529
Fragments arranged by size:
4529 1911 267 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1004 3872 1004
Size: 2868 3965
KasI G'GCGC_C
Cuts at: 4263 4511 4263
Size: 248 6585
KpnI G_GTAC'C
Cuts at: 1241 3853 1241
Size: 2612 4221
MfeI C'AATT_G
Cuts at: 1537 1537
Size: 6833
MluI A'CGCG_T
Cuts at: 6266 6266
Size: 6833
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1355 3457 5504 5688 1355
Size: 2102 2047 184 2500
Fragments arranged by size:
2500 2102 2047 184
MscI TGG'CCA
Cuts at: 3769 4594 3769
Size: 825 6008
MslI CAynn'nnrTG
Cuts at: 736 2417 3015 3293 4822 4949 736
Size: 1681 598 278 1529 127 2620
Fragments arranged by size:
2620 1681 1529 598 278 127
MspA1I CmG'CkG
Cuts at: 1171 1354 2064 2251 2807 2937 3000 3944 4258
Size: 183 710 187 556 130 63 944 314
Cuts at: 4258 4618 5631 5876 1171
Size: 360 1013 245 2128
Fragments arranged by size:
2128 1013 944 710 556 360 314 245
187 183 130 63
NaeI GCC'GGC
Cuts at: 5014 5014
Size: 6833
NarI GG'CG_CC
Cuts at: 4264 4512 4264
Size: 248 6585
NcoI C'CATG_G
Cuts at: 1230 1230
Size: 6833
NgoMIV G'CCGG_C
Cuts at: 5012 5012
Size: 6833
NspI r_CATG'y
Cuts at: 1447 1672 2820 3712 3886 4386 5293 1447
Size: 225 1148 892 174 500 907 2987
Fragments arranged by size:
2987 1148 907 892 500 225 174
PciI A'CATG_T
Cuts at: 3708 5289 3708
Size: 1581 5252
PflMI CCAn_nnn'nTGG
Cuts at: 460 1374 1938 460
Size: 914 564 5355
Fragments arranged by size:
5355 914 564
PfoI T'CCnGG_A
Cuts at: 1931 3227 3361 1931
Size: 1296 134 5403
Fragments arranged by size:
5403 1296 134
PmeI GTTT'AAAC
Cuts at: 3832 3832
Size: 6833
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5972 6004 5972
Size: 32 6801
PspXI vC'TCGA_Gb
Cuts at: 2348 2348
Size: 6833
PstI C_TGCA'G
Cuts at: 438 1535 1584 1778 2346 2942 3880 4565 438
Size: 1097 49 194 568 596 938 685 2706
Fragments arranged by size:
2706 1097 938 685 596 568 194 49
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6833
PvuII CAG'CTG
Cuts at: 1171 1354 2251 2807 2937 3000 4258 4618 1171
Size: 183 897 556 130 63 1258 360 3386
Fragments arranged by size:
3386 1258 897 556 360 183 130 63
RsrII CG'GwC_CG
Cuts at: 5028 5028
Size: 6833
SacI G_AGCT'C
Cuts at: 1436 3847 1436
Size: 2411 4422
SalI G'TCGA_C
Cuts at: 3870 3870
Size: 6833
SapI GCTCTTCn'nnn_
Cuts at: 496 1424 4856 5066 496
Size: 928 3432 210 2263
Fragments arranged by size:
3432 2263 928 210
SbfI CC_TGCA'GG
Cuts at: 3880 3880
Size: 6833
ScaI AGT'ACT
Cuts at: 2394 6350 2394
Size: 3956 2877
SfcI C'TryA_G
Cuts at: 33 434 987 1222 1531 1580 1774 2342 2635
Size: 401 553 235 309 49 194 568 293
Cuts at: 2635 2938 3876 4561 5554 5745 33
Size: 303 938 685 993 191 1121
Fragments arranged by size:
1121 993 938 685 568 553 401 309
303 293 235 194 191 49
SfoI GGC'GCC
Cuts at: 4265 4513 4265
Size: 248 6585
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6833
SmaI CCC'GGG
Cuts at: 3855 3855
Size: 6833
SmlI C'TyrA_G
Cuts at: 2266 2348 2450 3034 3988 4903 5395 5657 2266
Size: 82 102 584 954 915 492 262 3442
Fragments arranged by size:
3442 954 915 584 492 262 102 82
SnaBI TAC'GTA
Cuts at: 3825 3825
Size: 6833
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6833
SphI G_CATG'C
Cuts at: 1447 1672 3886 4386 1447
Size: 225 2214 500 3894
Fragments arranged by size:
3894 2214 500 225
SspI AAT'ATT
Cuts at: 912 1155 2694 2731 3262 912
Size: 243 1539 37 531 4483
Fragments arranged by size:
4483 1539 531 243 37
StuI AGG'CCT
Cuts at: 923 923
Size: 6833
StyI C'CwwG_G
Cuts at: 472 677 875 1230 3375 3967 4321 472
Size: 205 198 355 2145 592 354 2984
Fragments arranged by size:
2984 2145 592 355 354 205 198
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3407 4416 4549 5008 5217 3407
Size: 1009 133 459 209 5023
Fragments arranged by size:
5023 1009 459 209 133
TatI w'GTAC_w
Cuts at: 2392 6348 2392
Size: 3956 2877
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2701 2733 6350 6382 2701
Size: 32 3617 32 3152
Fragments arranged by size:
3617 3152 32 32
Tth111I GACn'n_nGTC
Cuts at: 4630 4630
Size: 6833
XbaI T'CTAG_A
Cuts at: 1083 3864 1083
Size: 2781 4052
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2681 4328 2681
Size: 1647 5186
XhoI C'TCGA_G
Cuts at: 2348 2348
Size: 6833
XmaI C'CCGG_G
Cuts at: 3853 3853
Size: 6833
XmnI GAAnn'nnTTC
Cuts at: 1859 1859
Size: 6833
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII
BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PflMI PfoI PmeI PpiI PspXI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI
Enzymes that do not cut:
AatII AflII AhdI AlfI AloI ApaI AscI BbvCI
BfrBI BmgBI BmtI BsiWI BsrGI BssHII BstZ17I ClaI
EcoRV FseI FspAI HindIII HpaI NdeI NheI NotI
NruI NsiI PacI PasI PmlI PpuMI PshAI PsiI
PspOMI PsrI SacII SanDI SexAI SfiI SgrAI SrfI
SwaI ZraI