Restriction map for
pF1KSDB2508
(Circular) (Six-base) MAPSORT of: pF1KSDB2508.seq Check: 3315 from: 1 to: 3851
pF1KSDB2508 3851 bp
With 182 enzymes: SgfI *
June 20, 2008 10:36 ..
AccI GT'mk_AC
Cuts at: 889 889
Size: 3851
Acc65I G'GTAC_C
Cuts at: 867 867
Size: 3851
AclI AA'CG_TT
Cuts at: 3691 3691
Size: 3851
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 514 1676 2108 2855 3123 514
Size: 1162 432 747 268 1242
Fragments arranged by size:
1242 1162 747 432 268
AfeI AGC'GCT
Cuts at: 189 189
Size: 3851
AflIII A'CryG_T
Cuts at: 335 1203 2307 3284 335
Size: 868 1104 977 902
Fragments arranged by size:
1104 977 902 868
AgeI A'CCGG_T
Cuts at: 2239 2239
Size: 3851
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 208 208
Size: 3851
AlwNI CAG_nnn'CTG
Cuts at: 321 673 747 1156 2723 321
Size: 352 74 409 1567 1449
Fragments arranged by size:
1567 1449 409 352 74
ApaLI G'TGCA_C
Cuts at: 258 349 2621 258
Size: 91 2272 1488
Fragments arranged by size:
2272 1488 91
ApoI r'AATT_y
Cuts at: 855 3204 3460 855
Size: 2349 256 1246
Fragments arranged by size:
2349 1246 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3851
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3851
AvaI C'yCGr_G
Cuts at: 177 871 177
Size: 694 3157
BamHI G'GATC_C
Cuts at: 622 876 622
Size: 254 3597
BanI G'GyrC_C
Cuts at: 161 235 301 867 1281 1529 1564 161
Size: 74 66 566 414 248 35 2448
Fragments arranged by size:
2448 566 414 248 74 66 35
BanII G_rGCy'C
Cuts at: 178 278 782 865 1895 178
Size: 100 504 83 1030 2134
Fragments arranged by size:
2134 1030 504 100 83
BbeI G_GCGC'C
Cuts at: 165 239 1285 1533 165
Size: 74 1046 248 2483
Fragments arranged by size:
2483 1046 248 74
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 249 283 249
Size: 34 3817
BciVI GTATCCnnnnn_n'
Cuts at: 1749 2510 3851 1749
Size: 761 1341 1749
Fragments arranged by size:
1749 1341 761
BclI T'GATC_A
Cuts at: 1371 1371
Size: 3851
BglI GCCn_nnn'nGGC
Cuts at: 159 182 159
Size: 23 3828
BglII A'GATC_T
Cuts at: 1366 3403 1366
Size: 2037 1814
BlpI GC'TnA_GC
Cuts at: 963 963
Size: 3851
Bme1580I G_kGCm'C
Cuts at: 262 353 1476 1569 2625 262
Size: 91 1123 93 1056 1488
Fragments arranged by size:
1488 1123 1056 93 91
BmgBI CAC'GTC
Cuts at: 336 336
Size: 3851
BmrI ACTGGGnnnn_n'
Cuts at: 1063 1144 1479 3623 1063
Size: 81 335 2144 1291
Fragments arranged by size:
2144 1291 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 774 2218 774
Size: 1444 2407
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 769 769
Size: 3851
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1027 1906 2398 2696 1027
Size: 879 492 298 2182
Fragments arranged by size:
2182 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 333 3555 333
Size: 3222 629
BsaAI yAC'GTr
Cuts at: 843 1204 843
Size: 361 3490
BsaBI GATnn'nnATC
Cuts at: 6 1365 3402 6
Size: 1359 2037 455
Fragments arranged by size:
2037 1359 455
BsaHI Gr'CG_yC
Cuts at: 162 236 1282 1530 2232 3728 162
Size: 74 1046 248 702 1496 285
Fragments arranged by size:
1496 1046 702 285 248 74
BsaWI w'CCGG_w
Cuts at: 1040 1561 2239 2513 2660 3071 1040
Size: 521 678 274 147 411 1820
Fragments arranged by size:
1820 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 186 751 186
Size: 565 3286
BseYI C'CCAG_C
Cuts at: 321 593 1276 2034 2611 3107 321
Size: 272 683 758 577 496 1065
Fragments arranged by size:
1065 758 683 577 496 272
BsiEI CG_ry'CG
Cuts at: 104 1393 1439 2647 104
Size: 1289 46 1208 1308
Fragments arranged by size:
1308 1289 1208 46
BsiHKAI G_wGCw'C
Cuts at: 262 353 865 1643 1833 2625 262
Size: 91 512 778 190 792 1488
Fragments arranged by size:
1488 792 778 512 190 91
Bsp1286I G_dGCh'C
Cuts at: 178 262 278 353 782 865 1476 1569 1643
Size: 84 16 75 429 83 611 93 74
Cuts at: 1643 1833 1895 2625 178
Size: 190 62 730 1404
Fragments arranged by size:
1404 730 611 429 190 93 84 83
75 74 62 16
BspEI T'CCGG_A
Cuts at: 3071 3071
Size: 3851
BspHI T'CATG_A
Cuts at: 3027 3027
Size: 3851
BspMI ACCTGCnnnn'nnnn_
Cuts at: 368 901 1115 1417 1798 368
Size: 533 214 302 381 2421
Fragments arranged by size:
2421 533 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2143 2197 3681 3846 50
Size: 2093 54 1484 165 55
Fragments arranged by size:
2093 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1763 1763
Size: 3851
BsrFI r'CCGG_y
Cuts at: 164 1849 2030 2239 164
Size: 1685 181 209 1776
Fragments arranged by size:
1776 1685 209 181
BssSI C'ACGA_G
Cuts at: 2122 2480 2122
Size: 358 3493
BstAPI GCAn_nnn'nTGC
Cuts at: 1228 1228
Size: 3851
BstBI TT'CG_AA
Cuts at: 110 2212 110
Size: 2102 1749
BstYI r'GATC_y
Cuts at: 1 622 876 1343 1366 1701 1947 2959 3045
Size: 621 254 467 23 335 246 1012 86
Cuts at: 3045 3057 3403 1
Size: 12 346 449
Fragments arranged by size:
1012 621 467 449 346 335 254 246
86 23 12
BtgI C'CryG_G
Cuts at: 294 618 695 294
Size: 324 77 3450
Fragments arranged by size:
3450 324 77
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 396 1801 1982 2145 396
Size: 1405 181 163 2102
Fragments arranged by size:
2102 1405 181 163
BtsI GCAGTG_nn'
Cuts at: 260 547 1094 260
Size: 287 547 3017
Fragments arranged by size:
3017 547 287
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 537 572 537
Size: 35 3816
DraI TTT'AAA
Cuts at: 850 850
Size: 3851
DrdI GACnn_nn'nnGTC
Cuts at: 1557 2415 1557
Size: 858 2993
EaeI y'GGCC_r
Cuts at: 692 1436 1610 2001 2028 3373 692
Size: 744 174 391 27 1345 1170
Fragments arranged by size:
1345 1170 744 391 174 27
EagI C'GGCC_G
Cuts at: 1436 1436
Size: 3851
EarI CTCTTCn'nnn_
Cuts at: 728 764 1874 2084 728
Size: 36 1110 210 2495
Fragments arranged by size:
2495 1110 210 36
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 183 805 2059 2369 2515 3661 183
Size: 622 1254 310 146 1146 373
Fragments arranged by size:
1254 1146 622 373 310 146
EcoICRI GAG'CTC
Cuts at: 863 863
Size: 3851
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 514 769 1676 2108 2855 3123 514
Size: 255 907 432 747 268 1242
Fragments arranged by size:
1242 907 747 432 268 255
EcoO109I rG'GnC_Cy
Cuts at: 990 990
Size: 3851
EcoRI G'AATT_C
Cuts at: 855 855
Size: 3851
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1870 1870
Size: 3851
FspI TGC'GCA
Cuts at: 1632 1632
Size: 3851
HaeII r_GCGC'y
Cuts at: 165 191 239 256 748 1285 1533 2555 3534
Size: 26 48 17 492 537 248 1022 979
Cuts at: 3534 165
Size: 482
Fragments arranged by size:
1022 979 537 492 482 248 48 26
17
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 489 521 614 646 671 703 1693 1723 1725
Size: 32 93 32 25 32 990 30 2
Cuts at: 1725 1755 489
Size: 30 2585
Fragments arranged by size:
2585 990 93 32 32 32 30 30
25 2
HincII GTy'rAC
Cuts at: 890 890
Size: 3851
KasI G'GCGC_C
Cuts at: 161 235 1281 1529 161
Size: 74 1046 248 2483
Fragments arranged by size:
2483 1046 248 74
KpnI G_GTAC'C
Cuts at: 871 871
Size: 3851
MluI A'CGCG_T
Cuts at: 3284 3284
Size: 3851
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 356 2522 2706 356
Size: 2166 184 1501
Fragments arranged by size:
2166 1501 184
MscI TGG'CCA
Cuts at: 694 1612 694
Size: 918 2933
MslI CAynn'nnrTG
Cuts at: 1840 1967 1840
Size: 127 3724
MspA1I CmG'CkG
Cuts at: 962 1276 1636 2649 2894 962
Size: 314 360 1013 245 1919
Fragments arranged by size:
1919 1013 360 314 245
NaeI GCC'GGC
Cuts at: 166 2032 166
Size: 1866 1985
NarI GG'CG_CC
Cuts at: 162 236 1282 1530 162
Size: 74 1046 248 2483
Fragments arranged by size:
2483 1046 248 74
NcoI C'CATG_G
Cuts at: 618 618
Size: 3851
NgoMIV G'CCGG_C
Cuts at: 164 2030 164
Size: 1866 1985
NspI r_CATG'y
Cuts at: 904 1404 2311 904
Size: 500 907 2444
Fragments arranged by size:
2444 907 500
PciI A'CATG_T
Cuts at: 2307 2307
Size: 3851
PflMI CCAn_nnn'nTGG
Cuts at: 321 321
Size: 3851
PmeI GTTT'AAAC
Cuts at: 850 850
Size: 3851
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2990 3022 2990
Size: 32 3819
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 766 798 766
Size: 32 3819
PstI C_TGCA'G
Cuts at: 542 898 1583 542
Size: 356 685 2810
Fragments arranged by size:
2810 685 356
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3851
PvuII CAG'CTG
Cuts at: 1276 1636 1276
Size: 360 3491
RsrII CG'GwC_CG
Cuts at: 2046 2046
Size: 3851
SacI G_AGCT'C
Cuts at: 865 865
Size: 3851
SalI G'TCGA_C
Cuts at: 888 888
Size: 3851
SapI GCTCTTCn'nnn_
Cuts at: 1874 2084 1874
Size: 210 3641
SbfI CC_TGCA'GG
Cuts at: 898 898
Size: 3851
ScaI AGT'ACT
Cuts at: 3368 3368
Size: 3851
SexAI A'CCwGG_T
Cuts at: 659 659
Size: 3851
SfcI C'TryA_G
Cuts at: 33 538 894 1579 2572 2763 33
Size: 505 356 685 993 191 1121
Fragments arranged by size:
1121 993 685 505 356 191
SfoI GGC'GCC
Cuts at: 163 237 1283 1531 163
Size: 74 1046 248 2483
Fragments arranged by size:
2483 1046 248 74
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3851
SgrAI Cr'CCGG_yG
Cuts at: 164 164
Size: 3851
SmaI CCC'GGG
Cuts at: 179 873 179
Size: 694 3157
SmlI C'TyrA_G
Cuts at: 1006 1921 2413 2675 1006
Size: 915 492 262 2182
Fragments arranged by size:
2182 915 492 262
SnaBI TAC'GTA
Cuts at: 843 843
Size: 3851
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3851
SphI G_CATG'C
Cuts at: 904 1404 904
Size: 500 3351
StyI C'CwwG_G
Cuts at: 618 985 1339 618
Size: 367 354 3130
Fragments arranged by size:
3130 367 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1434 1567 2026 2235 1434
Size: 133 459 209 3050
Fragments arranged by size:
3050 459 209 133
TatI w'GTAC_w
Cuts at: 3366 3366
Size: 3851
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3368 3400 3368
Size: 32 3819
Tth111I GACn'n_nGTC
Cuts at: 1648 1648
Size: 3851
XbaI T'CTAG_A
Cuts at: 882 882
Size: 3851
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1346 1346
Size: 3851
XmaI C'CCGG_G
Cuts at: 177 871 177
Size: 694 3157
XmnI GAAnn'nnTTC
Cuts at: 370 370
Size: 3851
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI BtgI BtgZI BtsI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PflMI PmeI PpiI PsrI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AloI ApaI AscI
AvrII BaeI BbsI BbvCI BfrBI BmtI Bpu10I BsaXI
BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI
BstZ17I Bsu36I ClaI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PasI PfoI PmlI PpuMI PshAI PsiI PspOMI
PspXI SacII SanDI SfiI SrfI SspI StuI SwaI
XhoI ZraI