Restriction map for
pF1KSDB1496
(Circular) (Six-base) MAPSORT of: pF1KSDB1496.seq Check: 7975 from: 1 to: 3968
pF1KSDB1496 3968 bp
With 182 enzymes: SgfI *
June 20, 2008 10:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 682 682
Size: 3968
AccI GT'mk_AC
Cuts at: 1006 1006
Size: 3968
Acc65I G'GTAC_C
Cuts at: 984 984
Size: 3968
AclI AA'CG_TT
Cuts at: 3808 3808
Size: 3968
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1793 2225 2972 3240 1793
Size: 432 747 268 2521
Fragments arranged by size:
2521 747 432 268
AflIII A'CryG_T
Cuts at: 1320 2424 3401 1320
Size: 1104 977 1887
Fragments arranged by size:
1887 1104 977
AgeI A'CCGG_T
Cuts at: 2356 2356
Size: 3968
AlwNI CAG_nnn'CTG
Cuts at: 725 1273 2840 725
Size: 548 1567 1853
Fragments arranged by size:
1853 1567 548
ApaI G_GGCC'C
Cuts at: 168 378 168
Size: 210 3758
ApaLI G'TGCA_C
Cuts at: 2738 2738
Size: 3968
ApoI r'AATT_y
Cuts at: 972 3321 3577 972
Size: 2349 256 1363
Fragments arranged by size:
2349 1363 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3968
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3968
AvaI C'yCGr_G
Cuts at: 988 988
Size: 3968
BamHI G'GATC_C
Cuts at: 993 993
Size: 3968
BanI G'GyrC_C
Cuts at: 946 984 1398 1646 1681 946
Size: 38 414 248 35 3233
Fragments arranged by size:
3233 414 248 38 35
BanII G_rGCy'C
Cuts at: 168 378 982 2012 168
Size: 210 604 1030 2124
Fragments arranged by size:
2124 1030 604 210
BbeI G_GCGC'C
Cuts at: 1402 1650 1402
Size: 248 3720
BbvCI CC'TCA_GC
Cuts at: 389 389
Size: 3968
BciVI GTATCCnnnnn_n'
Cuts at: 1866 2627 3968 1866
Size: 761 1341 1866
Fragments arranged by size:
1866 1341 761
BclI T'GATC_A
Cuts at: 1488 1488
Size: 3968
BglII A'GATC_T
Cuts at: 422 1483 3520 422
Size: 1061 2037 870
Fragments arranged by size:
2037 1061 870
BlpI GC'TnA_GC
Cuts at: 1080 1080
Size: 3968
Bme1580I G_kGCm'C
Cuts at: 168 378 1593 1686 2742 168
Size: 210 1215 93 1056 1394
Fragments arranged by size:
1394 1215 1056 210 93
BmrI ACTGGGnnnn_n'
Cuts at: 1180 1261 1596 3740 1180
Size: 81 335 2144 1408
Fragments arranged by size:
2144 1408 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2335 2335
Size: 3968
Bpu10I CC'TnA_GC
Cuts at: 389 463 389
Size: 74 3894
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1144 2023 2515 2813 1144
Size: 879 492 298 2299
Fragments arranged by size:
2299 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 262 3672 262
Size: 3410 558
BsaAI yAC'GTr
Cuts at: 960 1321 960
Size: 361 3607
BsaBI GATnn'nnATC
Cuts at: 6 1482 3519 6
Size: 1476 2037 455
Fragments arranged by size:
2037 1476 455
BsaHI Gr'CG_yC
Cuts at: 1399 1647 2349 3845 1399
Size: 248 702 1496 1522
Fragments arranged by size:
1522 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1157 1678 2356 2630 2777 3188 1157
Size: 521 678 274 147 411 1937
Fragments arranged by size:
1937 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 523 553 523
Size: 30 3938
BseYI C'CCAG_C
Cuts at: 694 1393 2151 2728 3224 694
Size: 699 758 577 496 1438
Fragments arranged by size:
1438 758 699 577 496
BsiEI CG_ry'CG
Cuts at: 104 1510 1556 2764 104
Size: 1406 46 1208 1308
Fragments arranged by size:
1406 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 982 1760 1950 2742 982
Size: 778 190 792 2208
Fragments arranged by size:
2208 792 778 190
BsmI GAATG_Cn'
Cuts at: 768 768
Size: 3968
Bsp1286I G_dGCh'C
Cuts at: 168 378 982 1593 1686 1760 1950 2012 2742
Size: 210 604 611 93 74 190 62 730
Cuts at: 2742 168
Size: 1394
Fragments arranged by size:
1394 730 611 604 210 190 93 74
62
BspEI T'CCGG_A
Cuts at: 3188 3188
Size: 3968
BspHI T'CATG_A
Cuts at: 896 3144 896
Size: 2248 1720
BspMI ACCTGCnnnn'nnnn_
Cuts at: 682 1018 1232 1534 1915 682
Size: 336 214 302 381 2735
Fragments arranged by size:
2735 381 336 302 214
BsrBI CCG'CTC
Cuts at: 50 534 2260 2314 3798 3963 50
Size: 484 1726 54 1484 165 55
Fragments arranged by size:
1726 1484 484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1880 1880
Size: 3968
BsrFI r'CCGG_y
Cuts at: 406 1966 2147 2356 406
Size: 1560 181 209 2018
Fragments arranged by size:
2018 1560 209 181
BsrGI T'GTAC_A
Cuts at: 647 647
Size: 3968
BssSI C'ACGA_G
Cuts at: 2239 2597 2239
Size: 358 3610
BstAPI GCAn_nnn'nTGC
Cuts at: 1345 1345
Size: 3968
BstBI TT'CG_AA
Cuts at: 110 2329 110
Size: 2219 1749
BstYI r'GATC_y
Cuts at: 1 422 993 1460 1483 1818 2064 3076 3162
Size: 421 571 467 23 335 246 1012 86
Cuts at: 3162 3174 3520 1
Size: 12 346 449
Fragments arranged by size:
1012 571 467 449 421 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 125 757 125
Size: 632 3336
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1918 2099 2262 1918
Size: 181 163 3624
Fragments arranged by size:
3624 181 163
BtsI GCAGTG_nn'
Cuts at: 1211 1211
Size: 3968
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 765 800 765
Size: 35 3933
DraI TTT'AAA
Cuts at: 967 967
Size: 3968
DrdI GACnn_nn'nnGTC
Cuts at: 1674 2532 1674
Size: 858 3110
EaeI y'GGCC_r
Cuts at: 185 1553 1727 2118 2145 3490 185
Size: 1368 174 391 27 1345 663
Fragments arranged by size:
1368 1345 663 391 174 27
EagI C'GGCC_G
Cuts at: 1553 1553
Size: 3968
EarI CTCTTCn'nnn_
Cuts at: 1991 2201 1991
Size: 210 3758
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2176 2486 2632 3778 2176
Size: 310 146 1146 2366
Fragments arranged by size:
2366 1146 310 146
EcoICRI GAG'CTC
Cuts at: 980 980
Size: 3968
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1793 2225 2972 3240 1793
Size: 432 747 268 2521
Fragments arranged by size:
2521 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 164 165 374 375 459 1107 164
Size: 1 209 1 84 648 3025
Fragments arranged by size:
3025 648 209 84 1 1
EcoRI G'AATT_C
Cuts at: 972 972
Size: 3968
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1987 1987
Size: 3968
FspI TGC'GCA
Cuts at: 1749 1749
Size: 3968
HaeII r_GCGC'y
Cuts at: 1402 1650 2672 3651 1402
Size: 248 1022 979 1719
Fragments arranged by size:
1719 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1810 1840 1842 1872 1810
Size: 30 2 30 3906
Fragments arranged by size:
3906 30 30 2
HincII GTy'rAC
Cuts at: 1007 1007
Size: 3968
KasI G'GCGC_C
Cuts at: 1398 1646 1398
Size: 248 3720
KpnI G_GTAC'C
Cuts at: 988 988
Size: 3968
MluI A'CGCG_T
Cuts at: 3401 3401
Size: 3968
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 815 2639 2823 815
Size: 1824 184 1960
Fragments arranged by size:
1960 1824 184
MscI TGG'CCA
Cuts at: 187 1729 187
Size: 1542 2426
MslI CAynn'nnrTG
Cuts at: 313 895 1957 2084 313
Size: 582 1062 127 2197
Fragments arranged by size:
2197 1062 582 127
MspA1I CmG'CkG
Cuts at: 817 1079 1393 1753 2766 3011 817
Size: 262 314 360 1013 245 1774
Fragments arranged by size:
1774 1013 360 314 262 245
NaeI GCC'GGC
Cuts at: 2149 2149
Size: 3968
NarI GG'CG_CC
Cuts at: 1399 1647 1399
Size: 248 3720
NcoI C'CATG_G
Cuts at: 125 757 125
Size: 632 3336
NgoMIV G'CCGG_C
Cuts at: 2147 2147
Size: 3968
NspI r_CATG'y
Cuts at: 1021 1521 2428 1021
Size: 500 907 2561
Fragments arranged by size:
2561 907 500
PciI A'CATG_T
Cuts at: 2424 2424
Size: 3968
PmeI GTTT'AAAC
Cuts at: 967 967
Size: 3968
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3107 3139 3107
Size: 32 3936
PspOMI G'GGCC_C
Cuts at: 164 374 164
Size: 210 3758
PstI C_TGCA'G
Cuts at: 870 1015 1700 870
Size: 145 685 3138
Fragments arranged by size:
3138 685 145
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3968
PvuII CAG'CTG
Cuts at: 817 1393 1753 817
Size: 576 360 3032
Fragments arranged by size:
3032 576 360
RsrII CG'GwC_CG
Cuts at: 2163 2163
Size: 3968
SacI G_AGCT'C
Cuts at: 982 982
Size: 3968
SalI G'TCGA_C
Cuts at: 1005 1005
Size: 3968
SapI GCTCTTCn'nnn_
Cuts at: 1991 2201 1991
Size: 210 3758
SbfI CC_TGCA'GG
Cuts at: 1015 1015
Size: 3968
ScaI AGT'ACT
Cuts at: 3485 3485
Size: 3968
SfcI C'TryA_G
Cuts at: 33 866 1011 1696 2689 2880 33
Size: 833 145 685 993 191 1121
Fragments arranged by size:
1121 993 833 685 191 145
SfoI GGC'GCC
Cuts at: 1400 1648 1400
Size: 248 3720
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3968
SgrAI Cr'CCGG_yG
Cuts at: 406 406
Size: 3968
SmaI CCC'GGG
Cuts at: 990 990
Size: 3968
SmlI C'TyrA_G
Cuts at: 1123 2038 2530 2792 1123
Size: 915 492 262 2299
Fragments arranged by size:
2299 915 492 262
SnaBI TAC'GTA
Cuts at: 960 960
Size: 3968
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3968
SphI G_CATG'C
Cuts at: 1021 1521 1021
Size: 500 3468
StyI C'CwwG_G
Cuts at: 125 757 1102 1456 125
Size: 632 345 354 2637
Fragments arranged by size:
2637 632 354 345
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1551 1684 2143 2352 1551
Size: 133 459 209 3167
Fragments arranged by size:
3167 459 209 133
TatI w'GTAC_w
Cuts at: 647 3483 647
Size: 2836 1132
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 523 555 3485 3517 523
Size: 32 2930 32 974
Fragments arranged by size:
2930 974 32 32
Tth111I GACn'n_nGTC
Cuts at: 1765 1765
Size: 3968
XbaI T'CTAG_A
Cuts at: 999 999
Size: 3968
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1463 1463
Size: 3968
XmaI C'CCGG_G
Cuts at: 988 988
Size: 3968
XmnI GAAnn'nnTTC
Cuts at: 766 766
Size: 3968
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbvCI BciVI BclI BglII BlpI Bme1580I
BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstYI BtgI BtgZI BtsI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PmeI PpiI PspOMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BbsI BcgI BfrBI BglI BmgBI BmtI
BpmI BseRI BsgI BsiWI BsmBI BssHII BstEII BstXI
BstZ17I Bsu36I ClaI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PasI PflMI PfoI PmlI PpuMI PshAI PsiI
PspXI PsrI SacII SanDI SexAI SfiI SrfI SspI
StuI SwaI XhoI ZraI