Restriction map for
pF1KSDB1489
(Circular) (Six-base) MAPSORT of: pF1KSDB1489.seq Check: 9547 from: 1 to: 7691
pF1KSDB1489 7691 bp
With 182 enzymes: SgfI *
June 20, 2008 10:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 342 873 1764 1971 2012 342
Size: 531 891 207 41 6021
Fragments arranged by size:
6021 891 531 207 41
AccI GT'mk_AC
Cuts at: 4729 4729
Size: 7691
Acc65I G'GTAC_C
Cuts at: 4707 4707
Size: 7691
AclI AA'CG_TT
Cuts at: 7531 7531
Size: 7691
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 4121 5516 5948 6695 6963 4121
Size: 1395 432 747 268 4849
Fragments arranged by size:
4849 1395 747 432 268
AfeI AGC'GCT
Cuts at: 1915 3034 1915
Size: 1119 6572
AflIII A'CryG_T
Cuts at: 1393 3899 5043 6147 7124 1393
Size: 2506 1144 1104 977 1960
Fragments arranged by size:
2506 1960 1144 1104 977
AgeI A'CCGG_T
Cuts at: 6079 6079
Size: 7691
AleI CACnn'nnGTG
Cuts at: 1888 3523 1888
Size: 1635 6056
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2769 2779 2769
Size: 10 7681
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2286 2318 2286
Size: 32 7659
AlwNI CAG_nnn'CTG
Cuts at: 206 734 1570 1615 2347 2458 2613 2655 3125
Size: 528 836 45 732 111 155 42 470
Cuts at: 3125 3642 3964 4996 6563 206
Size: 517 322 1032 1567 1334
Fragments arranged by size:
1567 1334 1032 836 732 528 517 470
322 155 111 45 42
ApaI G_GGCC'C
Cuts at: 856 1213 1276 2290 3172 3790 4053 4153 856
Size: 357 63 1014 882 618 263 100 4394
Fragments arranged by size:
4394 1014 882 618 357 263 100 63
ApaLI G'TGCA_C
Cuts at: 1012 1999 2910 3526 4491 6461 1012
Size: 987 911 616 965 1970 2242
Fragments arranged by size:
2242 1970 987 965 911 616
ApoI r'AATT_y
Cuts at: 4695 7044 7300 4695
Size: 2349 256 5086
Fragments arranged by size:
5086 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 7691
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7691
AvaI C'yCGr_G
Cuts at: 222 276 430 847 885 1313 1330 2441 2888
Size: 54 154 417 38 428 17 1111 447
Cuts at: 2888 4134 4231 4464 4601 4711 222
Size: 1246 97 233 137 110 3202
Fragments arranged by size:
3202 1246 1111 447 428 417 233 154
137 110 97 54 38 17
BamHI G'GATC_C
Cuts at: 2535 4716 2535
Size: 2181 5510
BanI G'GyrC_C
Cuts at: 609 843 1297 1465 2298 3287 3834 4541 4707
Size: 234 454 168 833 989 547 707 166
Cuts at: 4707 5121 5369 5404 609
Size: 414 248 35 2896
Fragments arranged by size:
2896 989 833 707 547 454 414 248
234 168 166 35
BanII G_rGCy'C
Cuts at: 587 856 1213 1276 2068 2114 2290 2492 2510
Size: 269 357 63 792 46 176 202 18
Cuts at: 2510 2716 3172 3394 3770 3790 4053 4153 4297
Size: 206 456 222 376 20 263 100 144
Cuts at: 4297 4388 4585 4643 4705 5735 587
Size: 91 197 58 62 1030 2543
Fragments arranged by size:
2543 1030 792 456 376 357 269 263
222 206 202 197 176 144 100 91
63 62 58 46 20 18
BbeI G_GCGC'C
Cuts at: 613 4545 5125 5373 613
Size: 3932 580 248 2931
Fragments arranged by size:
3932 2931 580 248
BbsI GAAGACnn'nnnn_
Cuts at: 3210 3483 3608 3210
Size: 273 125 7293
Fragments arranged by size:
7293 273 125
BbvCI CC'TCA_GC
Cuts at: 869 3802 869
Size: 2933 4758
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1311 1345 3003 3037 1311
Size: 34 1658 34 5965
Fragments arranged by size:
5965 1658 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3100 5589 6350 7691 3100
Size: 2489 761 1341 3100
Fragments arranged by size:
3100 2489 1341 761
BclI T'GATC_A
Cuts at: 2093 5211 2093
Size: 3118 4573
BglI GCCn_nnn'nGGC
Cuts at: 730 2162 2418 3442 3785 3794 4048 730
Size: 1432 256 1024 343 9 254 4373
Fragments arranged by size:
4373 1432 1024 343 256 254 9
BglII A'GATC_T
Cuts at: 5206 7243 5206
Size: 2037 5654
BlpI GC'TnA_GC
Cuts at: 581 746 1976 2221 2486 2896 3318 3349 3354
Size: 165 1230 245 265 410 422 31 5
Cuts at: 3354 4803 581
Size: 1449 3469
Fragments arranged by size:
3469 1449 1230 422 410 265 245 165
31 5
Bme1580I G_kGCm'C
Cuts at: 848 856 1016 1103 1213 1276 1302 1339 1444
Size: 8 160 87 110 63 26 37 105
Cuts at: 1444 1468 1597 1644 1895 2003 2290 2914 3172
Size: 24 129 47 251 108 287 624 258
Cuts at: 3172 3292 3530 3733 3790 3839 4053 4153 4232
Size: 120 238 203 57 49 214 100 79
Cuts at: 4232 4274 4495 5316 5409 6465 848
Size: 42 221 821 93 1056 2074
Fragments arranged by size:
2074 1056 821 624 287 258 251 238
221 214 203 160 129 120 110 108
105 100 93 87 79 63 57 49
47 42 37 26 24 8
BmgBI CAC'GTC
Cuts at: 1223 1394 1553 1223
Size: 171 159 7361
Fragments arranged by size:
7361 171 159
BmrI ACTGGGnnnn_n'
Cuts at: 194 957 1668 2184 2598 4588 4903 4984 5319
Size: 763 711 516 414 1990 315 81 335
Cuts at: 5319 7463 194
Size: 2144 422
Fragments arranged by size:
2144 1990 763 711 516 422 414 335
315 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2133 3978 6058 2133
Size: 1845 2080 3766
Fragments arranged by size:
3766 2080 1845
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 316 720 788 1062 2465 2547 2635 2722 3160
Size: 404 68 274 1403 82 88 87 438
Cuts at: 3160 3408 4153 4321 4575 316
Size: 248 745 168 254 3432
Fragments arranged by size:
3432 1403 745 438 404 274 254 248
168 88 87 82 68
Bpu10I CC'TnA_GC
Cuts at: 522 869 1136 2580 3802 522
Size: 347 267 1444 1222 4411
Fragments arranged by size:
4411 1444 1222 347 267
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2035 4867 5746 6238 6536 2035
Size: 2832 879 492 298 3190
Fragments arranged by size:
3190 2832 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 2611 7395 2611
Size: 4784 2907
BsaAI yAC'GTr
Cuts at: 1539 1890 3238 4683 5044 1539
Size: 351 1348 1445 361 4186
Fragments arranged by size:
4186 1445 1348 361 351
BsaBI GATnn'nnATC
Cuts at: 6 3665 5205 7242 6
Size: 3659 1540 2037 455
Fragments arranged by size:
3659 2037 1540 455
BsaHI Gr'CG_yC
Cuts at: 610 4542 5122 5370 6072 7568 610
Size: 3932 580 248 702 1496 733
Fragments arranged by size:
3932 1496 733 702 580 248
BsaWI w'CCGG_w
Cuts at: 1468 4653 4880 5401 6079 6353 6500 6911 1468
Size: 3185 227 521 678 274 147 411 2248
Fragments arranged by size:
3185 2248 678 521 411 274 227 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 340 370 786 816 1021 1051 1463 1493 2286
Size: 30 416 30 205 30 412 30 793
Cuts at: 2286 2316 2463 2493 3757 3787 4435 4465 340
Size: 30 147 30 1264 30 648 30 3566
Fragments arranged by size:
3566 1264 793 648 416 412 205 147
30 30 30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 448 493 496 721 946 1042 1489 1624 2220
Size: 45 3 225 225 96 447 135 596
Cuts at: 2220 2323 2940 3298 3778 448
Size: 103 617 358 480 4361
Fragments arranged by size:
4361 617 596 480 447 358 225 225
135 103 96 45 3
BseYI C'CCAG_C
Cuts at: 132 855 1106 1275 1669 2337 2474 2552 2678
Size: 723 251 169 394 668 137 78 126
Cuts at: 2678 2960 3050 3337 3858 4111 4146 4213 4504
Size: 282 90 287 521 253 35 67 291
Cuts at: 4504 4522 5116 5874 6451 6947 132
Size: 18 594 758 577 496 876
Fragments arranged by size:
876 758 723 668 594 577 521 496
394 291 287 282 253 251 169 137
126 90 78 67 35 18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 526 534 583 1281 1341 1545 1930 2765 2917
Size: 8 49 698 60 204 385 835 152
Cuts at: 2917 4603 526
Size: 1686 3614
Fragments arranged by size:
3614 1686 835 698 385 204 152 60
49 8
BsiEI CG_ry'CG
Cuts at: 104 4057 4413 5233 5279 6487 104
Size: 3953 356 820 46 1208 1308
Fragments arranged by size:
3953 1308 1208 820 356 46
BsiHKAI G_wGCw'C
Cuts at: 233 692 1016 1054 1387 1730 2003 2068 2222
Size: 459 324 38 333 343 273 65 154
Cuts at: 2222 2510 2914 3394 3530 4388 4495 4561 4705
Size: 288 404 480 136 858 107 66 144
Cuts at: 4705 5483 5673 6465 233
Size: 778 190 792 1459
Fragments arranged by size:
1459 858 792 778 480 459 404 343
333 324 288 273 190 154 144 136
107 66 65 38
BsmI GAATG_Cn'
Cuts at: 1374 1608 2099 4365 1374
Size: 234 491 2266 4700
Fragments arranged by size:
4700 2266 491 234
BsmBI CGTCTCn'nnnn_
Cuts at: 2121 2121
Size: 7691
Bsp1286I G_dGCh'C
Cuts at: 233 587 692 848 856 1016 1054 1103 1213
Size: 354 105 156 8 160 38 49 110
Cuts at: 1213 1276 1302 1339 1387 1444 1468 1597 1644
Size: 63 26 37 48 57 24 129 47
Cuts at: 1644 1730 1895 2003 2068 2114 2222 2290 2492
Size: 86 165 108 65 46 108 68 202
Cuts at: 2492 2510 2716 2914 3172 3292 3394 3530 3733
Size: 18 206 198 258 120 102 136 203
Cuts at: 3733 3770 3790 3839 4053 4153 4232 4274 4297
Size: 37 20 49 214 100 79 42 23
Cuts at: 4297 4388 4495 4561 4585 4643 4705 5316 5409
Size: 91 107 66 24 58 62 611 93
Cuts at: 5409 5483 5673 5735 6465 233
Size: 74 190 62 730 1459
Fragments arranged by size:
1459 730 611 354 258 214 206 203
202 198 190 165 160 156 136 129
120 110 108 108 107 105 102 100
93 91 86 79 74 68 66 65
63 62 62 58 57 49 49 48
47 46 42 38 37 37 26 24
24 23 20 18 8
BspEI T'CCGG_A
Cuts at: 6911 6911
Size: 7691
BspHI T'CATG_A
Cuts at: 6867 6867
Size: 7691
BspMI ACCTGCnnnn'nnnn_
Cuts at: 338 342 421 873 1102 1764 1838 1971 2012
Size: 4 79 452 229 662 74 133 41
Cuts at: 2012 4741 4955 5257 5638 338
Size: 2729 214 302 381 2391
Fragments arranged by size:
2729 2391 662 452 381 302 229 214
133 79 74 41 4
BsrBI CCG'CTC
Cuts at: 50 3285 3331 4482 5983 6037 7521 7686 50
Size: 3235 46 1151 1501 54 1484 165 55
Fragments arranged by size:
3235 1501 1484 1151 165 55 54 46
BsrDI GCAATG_nn'
Cuts at: 903 2541 5603 903
Size: 1638 3062 2991
Fragments arranged by size:
3062 2991 1638
BsrFI r'CCGG_y
Cuts at: 724 781 5689 5870 6079 724
Size: 57 4908 181 209 2336
Fragments arranged by size:
4908 2336 209 181 57
BssSI C'ACGA_G
Cuts at: 2875 3615 3781 4381 5962 6320 2875
Size: 740 166 600 1581 358 4246
Fragments arranged by size:
4246 1581 740 600 358 166
BstAPI GCAn_nnn'nTGC
Cuts at: 367 734 1011 1124 1749 1779 5068 367
Size: 367 277 113 625 30 3289 2990
Fragments arranged by size:
3289 2990 625 367 277 113 30
BstBI TT'CG_AA
Cuts at: 110 6052 110
Size: 5942 1749
BstXI CCAn_nnnn'nTGG
Cuts at: 840 4089 4290 840
Size: 3249 201 4241
Fragments arranged by size:
4241 3249 201
BstYI r'GATC_y
Cuts at: 1 2535 3638 3666 4162 4716 5183 5206 5541
Size: 2534 1103 28 496 554 467 23 335
Cuts at: 5541 5787 6799 6885 6897 7243 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
2534 1103 1012 554 496 467 449 346
335 246 86 28 23 12
Bsu36I CC'TnA_GG
Cuts at: 1608 3791 1608
Size: 2183 5508
BtgI C'CryG_G
Cuts at: 1363 1436 2531 3374 3839 4124 4430 1363
Size: 73 1095 843 465 285 306 4624
Fragments arranged by size:
4624 1095 843 465 306 285 73
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 255 2057 5641 5822 5985 255
Size: 1802 3584 181 163 1961
Fragments arranged by size:
3584 1961 1802 181 163
BtsI GCAGTG_nn'
Cuts at: 2753 3518 3528 3814 4934 2753
Size: 765 10 286 1120 5510
Fragments arranged by size:
5510 1120 765 286 10
DraI TTT'AAA
Cuts at: 4690 4690
Size: 7691
DraIII CAC_nnn'GTG
Cuts at: 1020 2037 1020
Size: 1017 6674
DrdI GACnn_nn'nnGTC
Cuts at: 5397 6255 5397
Size: 858 6833
EaeI y'GGCC_r
Cuts at: 176 469 714 967 1340 1733 2429 3074 3362
Size: 293 245 253 373 393 696 645 288
Cuts at: 3362 4054 4433 5276 5450 5841 5868 7213 176
Size: 692 379 843 174 391 27 1345 654
Fragments arranged by size:
1345 843 696 692 654 645 393 391
379 373 293 288 253 245 174 27
EagI C'GGCC_G
Cuts at: 4054 5276 4054
Size: 1222 6469
EarI CTCTTCn'nnn_
Cuts at: 470 935 1492 1840 2206 3475 5714 5924 470
Size: 465 557 348 366 1269 2239 210 2237
Fragments arranged by size:
2239 2237 1269 557 465 366 348 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 466 3021 3860 4008 5899 6209 6355 7501 466
Size: 2555 839 148 1891 310 146 1146 656
Fragments arranged by size:
2555 1891 1146 839 656 310 148 146
EcoICRI GAG'CTC
Cuts at: 2066 2508 3392 4386 4703 2066
Size: 442 884 994 317 5054
Fragments arranged by size:
5054 994 884 442 317
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 316 720 788 1062 2465 2547 2635 2722 3160
Size: 404 68 274 1403 82 88 87 438
Cuts at: 3160 3408 4121 4153 4321 4575 5516 5948 6695
Size: 248 713 32 168 254 941 432 747
Cuts at: 6695 6963 316
Size: 268 1044
Fragments arranged by size:
1403 1044 941 747 713 438 432 404
274 268 254 248 168 88 87 82
68 32
EcoNI CCTnn'n_nnAGG
Cuts at: 1735 1875 2209 2309 2796 3363 3472 3754 1735
Size: 140 334 100 487 567 109 282 5672
Fragments arranged by size:
5672 567 487 334 282 140 109 100
EcoO109I rG'GnC_Cy
Cuts at: 239 255 526 594 731 808 852 1037 1073
Size: 16 271 68 137 77 44 185 36
Cuts at: 1073 1132 1201 1209 1238 1827 2286 2420 2691
Size: 59 69 8 29 589 459 134 271
Cuts at: 2691 3169 3477 3787 4049 4064 4150 4830 239
Size: 478 308 310 262 15 86 680 3100
Fragments arranged by size:
3100 680 589 478 459 310 308 271
271 262 185 137 134 86 77 69
68 59 44 36 29 16 15 8
EcoRI G'AATT_C
Cuts at: 4695 4695
Size: 7691
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 5710 5710
Size: 7691
FspI TGC'GCA
Cuts at: 360 1783 3013 5472 360
Size: 1423 1230 2459 2579
Fragments arranged by size:
2579 2459 1423 1230
FspAI rTGC'GCAy
Cuts at: 360 3013 360
Size: 2653 5038
HaeII r_GCGC'y
Cuts at: 613 1416 1917 2331 2764 3036 3421 4220 4521
Size: 803 501 414 433 272 385 799 301
Cuts at: 4521 4545 5125 5373 6395 7374 613
Size: 24 580 248 1022 979 930
Fragments arranged by size:
1022 979 930 803 799 580 501 433
414 385 301 272 248 24
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 868 900 1021 1053 1753 1785 2099 2131 2341
Size: 32 121 32 700 32 314 32 210
Cuts at: 2341 2373 2520 2552 2563 2595 3232 3264 3757
Size: 32 147 32 11 32 637 32 493
Cuts at: 3757 3789 4245 4277 5533 5563 5565 5595 868
Size: 32 456 32 1256 30 2 30 2964
Fragments arranged by size:
2964 1256 700 637 493 456 314 210
147 121 32 32 32 32 32 32
32 32 32 32 30 30 11 2
HincII GTy'rAC
Cuts at: 405 3702 4730 405
Size: 3297 1028 3366
Fragments arranged by size:
3366 3297 1028
KasI G'GCGC_C
Cuts at: 609 4541 5121 5369 609
Size: 3932 580 248 2931
Fragments arranged by size:
3932 2931 580 248
KpnI G_GTAC'C
Cuts at: 4711 4711
Size: 7691
MluI A'CGCG_T
Cuts at: 7124 7124
Size: 7691
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 485 2885 3260 3275 4218 6362 6546 485
Size: 2400 375 15 943 2144 184 1630
Fragments arranged by size:
2400 2144 1630 943 375 184 15
MscI TGG'CCA
Cuts at: 178 1735 5452 178
Size: 1557 3717 2417
Fragments arranged by size:
3717 2417 1557
MslI CAynn'nnrTG
Cuts at: 1888 2500 3427 3523 4288 5680 5807 1888
Size: 612 927 96 765 1392 127 3772
Fragments arranged by size:
3772 1392 927 765 612 127 96
MspA1I CmG'CkG
Cuts at: 499 518 567 616 698 761 1061 1567 1628
Size: 19 49 49 82 63 300 506 61
Cuts at: 1628 2122 2427 2485 2552 2639 3633 3927 4802
Size: 494 305 58 67 87 994 294 875
Cuts at: 4802 5116 5476 6489 6734 499
Size: 314 360 1013 245 1456
Fragments arranged by size:
1456 1013 994 875 506 494 360 314
305 300 294 245 87 82 67 63
61 58 49 49 19
NaeI GCC'GGC
Cuts at: 726 783 5872 726
Size: 57 5089 2545
Fragments arranged by size:
5089 2545 57
NarI GG'CG_CC
Cuts at: 610 4542 5122 5370 610
Size: 3932 580 248 2931
Fragments arranged by size:
3932 2931 580 248
NcoI C'CATG_G
Cuts at: 1363 3374 3839 1363
Size: 2011 465 5215
Fragments arranged by size:
5215 2011 465
NgoMIV G'CCGG_C
Cuts at: 724 781 5870 724
Size: 57 5089 2545
Fragments arranged by size:
5089 2545 57
NspI r_CATG'y
Cuts at: 1292 1428 1752 1770 3201 3903 4744 5244 6151
Size: 136 324 18 1431 702 841 500 907
Cuts at: 6151 1292
Size: 2832
Fragments arranged by size:
2832 1431 907 841 702 500 324 136
18
PasI CC'CwG_GG
Cuts at: 1205 2236 3293 4046 4510 1205
Size: 1031 1057 753 464 4386
Fragments arranged by size:
4386 1057 1031 753 464
PciI A'CATG_T
Cuts at: 3899 6147 3899
Size: 2248 5443
PflMI CCAn_nnn'nTGG
Cuts at: 399 1650 2613 3558 3820 4657 399
Size: 1251 963 945 262 837 3433
Fragments arranged by size:
3433 1251 963 945 837 262
PfoI T'CCnGG_A
Cuts at: 286 1039 1075 3551 286
Size: 753 36 2476 4426
Fragments arranged by size:
4426 2476 753 36
PmeI GTTT'AAAC
Cuts at: 4690 4690
Size: 7691
PmlI CAC'GTG
Cuts at: 3238 3238
Size: 7691
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2286 2318 6830 6862 2286
Size: 32 4512 32 3115
Fragments arranged by size:
4512 3115 32 32
PpuMI rG'GwC_Cy
Cuts at: 255 1037 1073 1132 1201 1238 1827 2691 4064
Size: 782 36 59 69 37 589 864 1373
Cuts at: 4064 255
Size: 3882
Fragments arranged by size:
3882 1373 864 782 589 69 59 37
36
PspOMI G'GGCC_C
Cuts at: 852 1209 1272 2286 3168 3786 4049 4149 852
Size: 357 63 1014 882 618 263 100 4394
Fragments arranged by size:
4394 1014 882 618 357 263 100 63
PspXI vC'TCGA_Gb
Cuts at: 1330 1330
Size: 7691
PstI C_TGCA'G
Cuts at: 254 760 981 1145 1159 1793 3177 3638 4010
Size: 506 221 164 14 634 1384 461 372
Cuts at: 4010 4738 5423 254
Size: 728 685 2522
Fragments arranged by size:
2522 1384 728 685 634 506 461 372
221 164 14
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7691
PvuII CAG'CTG
Cuts at: 567 761 1061 1567 2122 2485 2552 3633 3927
Size: 194 300 506 555 363 67 1081 294
Cuts at: 3927 5116 5476 567
Size: 1189 360 2782
Fragments arranged by size:
2782 1189 1081 555 506 363 360 300
294 194 67
RsrII CG'GwC_CG
Cuts at: 5886 5886
Size: 7691
SacI G_AGCT'C
Cuts at: 2068 2510 3394 4388 4705 2068
Size: 442 884 994 317 5054
Fragments arranged by size:
5054 994 884 442 317
SalI G'TCGA_C
Cuts at: 4728 4728
Size: 7691
SanDI GG'GwC_CC
Cuts at: 1073 1201 1238 4064 1073
Size: 128 37 2826 4700
Fragments arranged by size:
4700 2826 128 37
SapI GCTCTTCn'nnn_
Cuts at: 1840 5714 5924 1840
Size: 3874 210 3607
Fragments arranged by size:
3874 3607 210
SbfI CC_TGCA'GG
Cuts at: 1793 4738 1793
Size: 2945 4746
ScaI AGT'ACT
Cuts at: 319 7208 319
Size: 6889 802
SexAI A'CCwGG_T
Cuts at: 398 2033 398
Size: 1635 6056
SfcI C'TryA_G
Cuts at: 33 250 756 977 1141 1155 1789 1818 3173
Size: 217 506 221 164 14 634 29 1355
Cuts at: 3173 3634 4006 4734 5419 6412 6603 33
Size: 461 372 728 685 993 191 1121
Fragments arranged by size:
1355 1121 993 728 685 634 506 461
372 221 217 191 164 29 14
SfoI GGC'GCC
Cuts at: 611 4543 5123 5371 611
Size: 3932 580 248 2931
Fragments arranged by size:
3932 2931 580 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7691
SmaI CCC'GGG
Cuts at: 849 1315 2443 2890 4713 849
Size: 466 1128 447 1823 3827
Fragments arranged by size:
3827 1823 1128 466 447
SmlI C'TyrA_G
Cuts at: 1330 2050 4846 5761 6253 6515 1330
Size: 720 2796 915 492 262 2506
Fragments arranged by size:
2796 2506 915 720 492 262
SnaBI TAC'GTA
Cuts at: 4683 4683
Size: 7691
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7691
SphI G_CATG'C
Cuts at: 1752 4744 5244 1752
Size: 2992 500 4199
Fragments arranged by size:
4199 2992 500
StuI AGG'CCT
Cuts at: 1123 2056 2977 1123
Size: 933 921 5837
Fragments arranged by size:
5837 933 921
StyI C'CwwG_G
Cuts at: 1363 1644 1736 2210 2394 2687 3374 3839 4825
Size: 281 92 474 184 293 687 465 986
Cuts at: 4825 5179 1363
Size: 354 3875
Fragments arranged by size:
3875 986 687 474 465 354 293 281
184 92
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2166 2712 2952 4227 5274 5407 5866 6075 2166
Size: 546 240 1275 1047 133 459 209 3782
Fragments arranged by size:
3782 1275 1047 546 459 240 209 133
TatI w'GTAC_w
Cuts at: 317 2379 2670 7206 317
Size: 2062 291 4536 802
Fragments arranged by size:
4536 2062 802 291
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 340 372 786 814 818 846 1171 1203 1463
Size: 32 414 28 4 28 325 32 260
Cuts at: 1463 1495 4435 4467 7208 7240 340
Size: 32 2940 32 2741 32 791
Fragments arranged by size:
2940 2741 791 414 325 260 32 32
32 32 32 28 28 4
Tth111I GACn'n_nGTC
Cuts at: 5488 5488
Size: 7691
XbaI T'CTAG_A
Cuts at: 4722 4722
Size: 7691
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 679 1370 2424 3372 4177 5186 679
Size: 691 1054 948 805 1009 3184
Fragments arranged by size:
3184 1054 1009 948 805 691
XhoI C'TCGA_G
Cuts at: 1330 1330
Size: 7691
XmaI C'CCGG_G
Cuts at: 847 1313 2441 2888 4711 847
Size: 466 1128 447 1823 3827
Fragments arranged by size:
3827 1823 1128 466 447
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
FspAI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PspOMI PspXI PstI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SnaBI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AflII AhdI AscI AvrII BaeI BfrBI BmtI
BsiWI BsrGI BssHII BstEII BstZ17I ClaI CspCI EcoRV
FseI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PshAI PsiI PsrI SacII SfiI SgrAI
SrfI SspI SwaI XmnI ZraI