Restriction map for
pF1KSDB1483
(Circular) (Six-base) MAPSORT of: pF1KSDB1483.seq Check: 5381 from: 1 to: 6731
pF1KSDB1483 6731 bp
With 182 enzymes: SgfI *
June 20, 2008 10:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1671 2092 2418 1671
Size: 421 326 5984
Fragments arranged by size:
5984 421 326
AatII G_ACGT'C
Cuts at: 1547 3715 1547
Size: 2168 4563
AccI GT'mk_AC
Cuts at: 1295 2870 3769 1295
Size: 1575 899 4257
Fragments arranged by size:
4257 1575 899
Acc65I G'GTAC_C
Cuts at: 3747 3747
Size: 6731
AclI AA'CG_TT
Cuts at: 6571 6571
Size: 6731
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1750 2725 4556 4988 5735 6003 1750
Size: 975 1831 432 747 268 2478
Fragments arranged by size:
2478 1831 975 747 432 268
AflIII A'CryG_T
Cuts at: 2744 4083 5187 6164 2744
Size: 1339 1104 977 3311
Fragments arranged by size:
3311 1339 1104 977
AgeI A'CCGG_T
Cuts at: 5119 5119
Size: 6731
AleI CACnn'nnGTG
Cuts at: 1252 2518 3094 1252
Size: 1266 576 4889
Fragments arranged by size:
4889 1266 576
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1777 3112 1777
Size: 1335 5396
AlwNI CAG_nnn'CTG
Cuts at: 2515 3375 4036 5603 2515
Size: 860 661 1567 3643
Fragments arranged by size:
3643 1567 860 661
ApaLI G'TGCA_C
Cuts at: 201 1284 5501 201
Size: 1083 4217 1431
Fragments arranged by size:
4217 1431 1083
ApoI r'AATT_y
Cuts at: 365 697 2620 3505 3735 6084 6340 365
Size: 332 1923 885 230 2349 256 756
Fragments arranged by size:
2349 1923 885 756 332 256 230
AseI AT'TA_AT
Cuts at: 20 20
Size: 6731
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6731
AvaI C'yCGr_G
Cuts at: 1357 2204 2260 3588 3751 1357
Size: 847 56 1328 163 4337
Fragments arranged by size:
4337 1328 847 163 56
BamHI G'GATC_C
Cuts at: 2285 3398 3756 2285
Size: 1113 358 5260
Fragments arranged by size:
5260 1113 358
BanI G'GyrC_C
Cuts at: 299 334 1666 2677 3103 3747 4161 4409 4444
Size: 35 1332 1011 426 644 414 248 35
Cuts at: 4444 299
Size: 2586
Fragments arranged by size:
2586 1332 1011 644 426 414 248 35
35
BanII G_rGCy'C
Cuts at: 218 928 1367 1748 1979 1997 3215 3317 3595
Size: 710 439 381 231 18 1218 102 278
Cuts at: 3595 3694 3745 4775 218
Size: 99 51 1030 2174
Fragments arranged by size:
2174 1218 1030 710 439 381 278 231
102 99 51 18
BbeI G_GCGC'C
Cuts at: 303 1670 2681 4165 4413 303
Size: 1367 1011 1484 248 2621
Fragments arranged by size:
2621 1484 1367 1011 248
BbsI GAAGACnn'nnnn_
Cuts at: 1923 1923
Size: 6731
BbvCI CC'TCA_GC
Cuts at: 2797 2797
Size: 6731
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 508 542 2449 2483 508
Size: 34 1907 34 4756
Fragments arranged by size:
4756 1907 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4629 5390 6731 4629
Size: 761 1341 4629
Fragments arranged by size:
4629 1341 761
BclI T'GATC_A
Cuts at: 1217 1604 4251 1217
Size: 387 2647 3697
Fragments arranged by size:
3697 2647 387
BglI GCCn_nnn'nGGC
Cuts at: 188 1362 1712 2264 2530 2758 188
Size: 1174 350 552 266 228 4161
Fragments arranged by size:
4161 1174 552 350 266 228
BglII A'GATC_T
Cuts at: 2330 4246 6283 2330
Size: 1916 2037 2778
Fragments arranged by size:
2778 2037 1916
BlpI GC'TnA_GC
Cuts at: 3843 3843
Size: 6731
Bme1580I G_kGCm'C
Cuts at: 205 244 337 1288 1358 3106 4356 4449 5505
Size: 39 93 951 70 1748 1250 93 1056
Cuts at: 5505 205
Size: 1431
Fragments arranged by size:
1748 1431 1250 1056 951 93 93 70
39
BmgBI CAC'GTC
Cuts at: 441 2487 2520 3327 441
Size: 2046 33 807 3845
Fragments arranged by size:
3845 2046 807 33
BmrI ACTGGGnnnn_n'
Cuts at: 2058 3943 4024 4359 6503 2058
Size: 1885 81 335 2144 2286
Fragments arranged by size:
2286 2144 1885 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 237 5098 237
Size: 4861 1870
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 326 478 663 872 2817 3727 326
Size: 152 185 209 1945 910 3330
Fragments arranged by size:
3330 1945 910 209 185 152
Bpu10I CC'TnA_GC
Cuts at: 927 2797 927
Size: 1870 4861
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 811 1060 1870 2458 3241 3250 3562 3907 4786
Size: 249 810 588 783 9 312 345 879
Cuts at: 4786 5278 5576 811
Size: 492 298 1966
Fragments arranged by size:
1966 879 810 783 588 492 345 312
298 249 9
BsaI GGTCTCn'nnnn_
Cuts at: 1447 2779 6435 1447
Size: 1332 3656 1743
Fragments arranged by size:
3656 1743 1332
BsaAI yAC'GTr
Cuts at: 915 1299 3723 4084 915
Size: 384 2424 361 3562
Fragments arranged by size:
3562 2424 384 361
BsaBI GATnn'nnATC
Cuts at: 6 3124 4245 6282 6
Size: 3118 1121 2037 455
Fragments arranged by size:
3118 2037 1121 455
BsaHI Gr'CG_yC
Cuts at: 300 1544 1667 2678 3712 4162 4410 5112 6608
Size: 1244 123 1011 1034 450 248 702 1496
Cuts at: 6608 300
Size: 423
Fragments arranged by size:
1496 1244 1034 1011 702 450 423 248
123
BsaWI w'CCGG_w
Cuts at: 3071 3509 3920 4441 5119 5393 5540 5951 3071
Size: 438 411 521 678 274 147 411 3851
Fragments arranged by size:
3851 678 521 438 411 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 139 169 1558 1588 1762 1792 2599 2629 3200
Size: 30 1389 30 174 30 807 30 571
Cuts at: 3200 3230 139
Size: 30 3640
Fragments arranged by size:
3640 1389 807 571 174 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 226 589 778 1783 2352 3310 3322 3325 3346
Size: 363 189 1005 569 958 12 3 21
Cuts at: 3346 226
Size: 3611
Fragments arranged by size:
3611 1005 958 569 363 189 21 12
3
BseYI C'CCAG_C
Cuts at: 1570 1710 2029 3099 3524 4156 4914 5491 5987
Size: 140 319 1070 425 632 758 577 496
Cuts at: 5987 1570
Size: 2314
Fragments arranged by size:
2314 1070 758 632 577 496 425 319
140
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1195 1321 2453 2734 3072 3151 1195
Size: 126 1132 281 338 79 4775
Fragments arranged by size:
4775 1132 338 281 126 79
BsiEI CG_ry'CG
Cuts at: 104 1947 2814 3653 4273 4319 5527 104
Size: 1843 867 839 620 46 1208 1308
Fragments arranged by size:
1843 1308 1208 867 839 620 46
BsiHKAI G_wGCw'C
Cuts at: 205 218 1232 1288 1822 1886 3215 3317 3745
Size: 13 1014 56 534 64 1329 102 428
Cuts at: 3745 4523 4713 5505 205
Size: 778 190 792 1431
Fragments arranged by size:
1431 1329 1014 792 778 534 428 190
102 64 56 13
BsiWI C'GTAC_G
Cuts at: 2807 2807
Size: 6731
BsmI GAATG_Cn'
Cuts at: 2952 2952
Size: 6731
BsmBI CGTCTCn'nnnn_
Cuts at: 3703 3703
Size: 6731
Bsp1286I G_dGCh'C
Cuts at: 205 218 244 337 928 1232 1288 1358 1367
Size: 13 26 93 591 304 56 70 9
Cuts at: 1367 1748 1822 1886 1979 1997 3106 3215 3317
Size: 381 74 64 93 18 1109 109 102
Cuts at: 3317 3595 3694 3745 4356 4449 4523 4713 4775
Size: 278 99 51 611 93 74 190 62
Cuts at: 4775 5505 205
Size: 730 1431
Fragments arranged by size:
1431 1109 730 611 591 381 304 278
190 109 102 99 93 93 93 74
74 70 64 62 56 51 26 18
13 9
BspEI T'CCGG_A
Cuts at: 3071 3509 5951 3071
Size: 438 2442 3851
Fragments arranged by size:
3851 2442 438
BspHI T'CATG_A
Cuts at: 2999 5907 2999
Size: 2908 3823
BspMI ACCTGCnnnn'nnnn_
Cuts at: 562 1671 2092 2418 3781 3995 4297 4678 562
Size: 1109 421 326 1363 214 302 381 2615
Fragments arranged by size:
2615 1363 1109 421 381 326 302 214
BsrBI CCG'CTC
Cuts at: 50 221 340 2826 3351 5023 5077 6561 6726
Size: 171 119 2486 525 1672 54 1484 165
Cuts at: 6726 50
Size: 55
Fragments arranged by size:
2486 1672 1484 525 171 165 119 55
54
BsrDI GCAATG_nn'
Cuts at: 825 1470 3057 4643 825
Size: 645 1587 1586 2913
Fragments arranged by size:
2913 1587 1586 645
BsrFI r'CCGG_y
Cuts at: 296 4729 4910 5119 296
Size: 4433 181 209 1908
Fragments arranged by size:
4433 1908 209 181
BsrGI T'GTAC_A
Cuts at: 1778 1787 1856 1778
Size: 9 69 6653
Fragments arranged by size:
6653 69 9
BssSI C'ACGA_G
Cuts at: 418 1925 5002 5360 418
Size: 1507 3077 358 1789
Fragments arranged by size:
3077 1789 1507 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2006 4108 2006
Size: 2102 4629
BstBI TT'CG_AA
Cuts at: 110 5092 110
Size: 4982 1749
BstEII G'GTnAC_C
Cuts at: 781 781
Size: 6731
BstXI CCAn_nnnn'nTGG
Cuts at: 1389 1593 3099 1389
Size: 204 1506 5021
Fragments arranged by size:
5021 1506 204
BstYI r'GATC_y
Cuts at: 1 731 1559 2285 2330 3035 3299 3371 3398
Size: 730 828 726 45 705 264 72 27
Cuts at: 3398 3410 3756 4223 4246 4581 4827 5839 5925
Size: 12 346 467 23 335 246 1012 86
Cuts at: 5925 5937 6283 1
Size: 12 346 449
Fragments arranged by size:
1012 828 730 726 705 467 449 346
346 335 264 246 86 72 45 27
23 12 12
BtgI C'CryG_G
Cuts at: 125 183 704 878 1058 1270 1457 1592 1979
Size: 58 521 174 180 212 187 135 387
Cuts at: 1979 2498 2780 3039 125
Size: 519 282 259 3817
Fragments arranged by size:
3817 521 519 387 282 259 212 187
180 174 135 58
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1599 2003 2196 2490 3216 4681 4862 5025 1599
Size: 404 193 294 726 1465 181 163 3305
Fragments arranged by size:
3305 1465 726 404 294 193 181 163
BtsI GCAGTG_nn'
Cuts at: 2588 3163 3974 2588
Size: 575 811 5345
Fragments arranged by size:
5345 811 575
DraI TTT'AAA
Cuts at: 3730 3730
Size: 6731
DrdI GACnn_nn'nnGTC
Cuts at: 4437 5295 4437
Size: 858 5873
EaeI y'GGCC_r
Cuts at: 717 1373 2119 2335 2435 2522 2654 2765 2811
Size: 656 746 216 100 87 132 111 46
Cuts at: 2811 4316 4490 4881 4908 6253 717
Size: 1505 174 391 27 1345 1195
Fragments arranged by size:
1505 1345 1195 746 656 391 216 174
132 111 100 87 46 27
EagI C'GGCC_G
Cuts at: 2811 4316 2811
Size: 1505 5226
EarI CTCTTCn'nnn_
Cuts at: 811 1001 1007 3016 3681 4754 4964 811
Size: 190 6 2009 665 1073 210 2578
Fragments arranged by size:
2578 2009 1073 665 210 190 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4939 5249 5395 6541 4939
Size: 310 146 1146 5129
Fragments arranged by size:
5129 1146 310 146
EcoICRI GAG'CTC
Cuts at: 216 3213 3315 3743 216
Size: 2997 102 428 3204
Fragments arranged by size:
3204 2997 428 102
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 326 478 663 872 1750 2725 2817 3727 4556
Size: 152 185 209 878 975 92 910 829
Cuts at: 4556 4988 5735 6003 326
Size: 432 747 268 1054
Fragments arranged by size:
1054 975 910 878 829 747 432 268
209 185 152 92
EcoNI CCTnn'n_nnAGG
Cuts at: 320 1732 3268 320
Size: 1412 1536 3783
Fragments arranged by size:
3783 1536 1412
EcoO109I rG'GnC_Cy
Cuts at: 666 888 2109 2127 3870 666
Size: 222 1221 18 1743 3527
Fragments arranged by size:
3527 1743 1221 222 18
EcoRI G'AATT_C
Cuts at: 3505 3735 3505
Size: 230 6501
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1209 4750 1209
Size: 3541 3190
FspI TGC'GCA
Cuts at: 871 1753 1807 4512 871
Size: 882 54 2705 3090
Fragments arranged by size:
3090 2705 882 54
HaeII r_GCGC'y
Cuts at: 303 433 1631 1670 2681 3626 4165 4413 5435
Size: 130 1198 39 1011 945 539 248 1022
Cuts at: 5435 6414 303
Size: 979 620
Fragments arranged by size:
1198 1022 1011 979 945 620 539 248
130 39
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 139 171 826 858 1494 1526 1558 1590 2599
Size: 32 655 32 636 32 32 32 1009
Cuts at: 2599 2631 4573 4603 4605 4635 139
Size: 32 1942 30 2 30 2235
Fragments arranged by size:
2235 1942 1009 655 636 32 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1260 2646 3770 1260
Size: 1386 1124 4221
Fragments arranged by size:
4221 1386 1124
KasI G'GCGC_C
Cuts at: 299 1666 2677 4161 4409 299
Size: 1367 1011 1484 248 2621
Fragments arranged by size:
2621 1484 1367 1011 248
KpnI G_GTAC'C
Cuts at: 3751 3751
Size: 6731
MluI A'CGCG_T
Cuts at: 6164 6164
Size: 6731
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 556 5402 5586 556
Size: 4846 184 1701
Fragments arranged by size:
4846 1701 184
MscI TGG'CCA
Cuts at: 719 2337 2524 2767 4492 719
Size: 1618 187 243 1725 2958
Fragments arranged by size:
2958 1725 1618 243 187
MslI CAynn'nnrTG
Cuts at: 1063 1213 1225 1252 1330 1442 1519 2092 2518
Size: 150 12 27 78 112 77 573 426
Cuts at: 2518 3094 4720 4847 1063
Size: 576 1626 127 2947
Fragments arranged by size:
2947 1626 576 573 426 150 127 112
78 77 27 12
MspA1I CmG'CkG
Cuts at: 2154 2424 2933 3155 3171 3842 4156 4516 5529
Size: 270 509 222 16 671 314 360 1013
Cuts at: 5529 5774 2154
Size: 245 3111
Fragments arranged by size:
3111 1013 671 509 360 314 270 245
222 16
NaeI GCC'GGC
Cuts at: 298 4912 298
Size: 4614 2117
NarI GG'CG_CC
Cuts at: 300 1667 2678 4162 4410 300
Size: 1367 1011 1484 248 2621
Fragments arranged by size:
2621 1484 1367 1011 248
NcoI C'CATG_G
Cuts at: 125 183 1058 1457 1592 1979 2498 125
Size: 58 875 399 135 387 519 4358
Fragments arranged by size:
4358 875 519 399 387 135 58
NgoMIV G'CCGG_C
Cuts at: 296 4910 296
Size: 4614 2117
NspI r_CATG'y
Cuts at: 1125 1857 3784 4284 5191 1125
Size: 732 1927 500 907 2665
Fragments arranged by size:
2665 1927 907 732 500
PasI CC'CwG_GG
Cuts at: 480 537 480
Size: 57 6674
PciI A'CATG_T
Cuts at: 5187 5187
Size: 6731
PflMI CCAn_nnn'nTGG
Cuts at: 918 3705 918
Size: 2787 3944
PfoI T'CCnGG_A
Cuts at: 455 2833 3302 455
Size: 2378 469 3884
Fragments arranged by size:
3884 2378 469
PmeI GTTT'AAAC
Cuts at: 3730 3730
Size: 6731
PmlI CAC'GTG
Cuts at: 915 915
Size: 6731
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 643 675 2902 2934 5870 5902 643
Size: 32 2227 32 2936 32 1472
Fragments arranged by size:
2936 2227 1472 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 888 2109 2127 888
Size: 1221 18 5492
Fragments arranged by size:
5492 1221 18
PspXI vC'TCGA_Gb
Cuts at: 2260 3588 2260
Size: 1328 5403
PstI C_TGCA'G
Cuts at: 416 437 674 1151 3170 3778 4463 416
Size: 21 237 477 2019 608 685 2684
Fragments arranged by size:
2684 2019 685 608 477 237 21
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6731
PvuII CAG'CTG
Cuts at: 3155 3171 4156 4516 3155
Size: 16 985 360 5370
Fragments arranged by size:
5370 985 360 16
RsrII CG'GwC_CG
Cuts at: 2169 4926 2169
Size: 2757 3974
SacI G_AGCT'C
Cuts at: 218 3215 3317 3745 218
Size: 2997 102 428 3204
Fragments arranged by size:
3204 2997 428 102
SalI G'TCGA_C
Cuts at: 3768 3768
Size: 6731
SapI GCTCTTCn'nnn_
Cuts at: 3016 4754 4964 3016
Size: 1738 210 4783
Fragments arranged by size:
4783 1738 210
SbfI CC_TGCA'GG
Cuts at: 3778 3778
Size: 6731
ScaI AGT'ACT
Cuts at: 1306 6248 1306
Size: 4942 1789
SexAI A'CCwGG_T
Cuts at: 863 863
Size: 6731
SfcI C'TryA_G
Cuts at: 33 412 433 670 1147 3166 3774 4459 5452
Size: 379 21 237 477 2019 608 685 993
Cuts at: 5452 5643 33
Size: 191 1121
Fragments arranged by size:
2019 1121 993 685 608 477 379 237
191 21
SfiI GGCCn_nnn'nGGCC
Cuts at: 2530 2530
Size: 6731
SfoI GGC'GCC
Cuts at: 301 1668 2679 4163 4411 301
Size: 1367 1011 1484 248 2621
Fragments arranged by size:
2621 1484 1367 1011 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6731
SmaI CCC'GGG
Cuts at: 3753 3753
Size: 6731
SmlI C'TyrA_G
Cuts at: 826 1075 1885 2260 2473 3256 3265 3577 3588
Size: 249 810 375 213 783 9 312 11
Cuts at: 3588 3886 4801 5293 5555 826
Size: 298 915 492 262 2002
Fragments arranged by size:
2002 915 810 783 492 375 312 298
262 249 213 11 9
SnaBI TAC'GTA
Cuts at: 3723 3723
Size: 6731
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6731
SphI G_CATG'C
Cuts at: 1125 3784 4284 1125
Size: 2659 500 3572
Fragments arranged by size:
3572 2659 500
StuI AGG'CCT
Cuts at: 1531 2533 3389 1531
Size: 1002 856 4873
Fragments arranged by size:
4873 1002 856
StyI C'CwwG_G
Cuts at: 125 183 1058 1457 1592 1979 2498 2525 3865
Size: 58 875 399 135 387 519 27 1340
Cuts at: 3865 4219 125
Size: 354 2637
Fragments arranged by size:
2637 1340 875 519 399 387 354 135
58 27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 118 943 2715 3667 4314 4447 4906 5115 118
Size: 825 1772 952 647 133 459 209 1734
Fragments arranged by size:
1772 1734 952 825 647 459 209 133
TatI w'GTAC_w
Cuts at: 1304 1778 1787 1856 6246 1304
Size: 474 9 69 4390 1789
Fragments arranged by size:
4390 1789 474 69 9
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 899 931 2239 2271 6248 6280 899
Size: 32 1308 32 3977 32 1350
Fragments arranged by size:
3977 1350 1308 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 4528 4528
Size: 6731
XbaI T'CTAG_A
Cuts at: 3762 3762
Size: 6731
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 477 775 3702 4226 477
Size: 298 2927 524 2982
Fragments arranged by size:
2982 2927 524 298
XhoI C'TCGA_G
Cuts at: 2260 3588 2260
Size: 1328 5403
XmaI C'CCGG_G
Cuts at: 3751 3751
Size: 6731
XmnI GAAnn'nnTTC
Cuts at: 658 2917 3148 3427 658
Size: 2259 231 279 3962
Fragments arranged by size:
3962 2259 279 231
ZraI GAC'GTC
Cuts at: 1545 3713 1545
Size: 2168 4563
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PspXI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SmaI SmlI SnaBI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AloI ApaI AscI AvrII BaeI
BfrBI BmtI BssHII BstZ17I Bsu36I ClaI CspCI DraIII
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PshAI PsiI PspOMI PsrI
SacII SanDI SgrAI SrfI SspI SwaI