Restriction map for
pF1KSDB1453
(Circular) (Six-base) MAPSORT of: pF1KSDB1453.seq Check: 7659 from: 1 to: 4532
pF1KSDB1453 4532 bp
With 182 enzymes: SgfI *
June 20, 2008 10:36 ..
AatII G_ACGT'C
Cuts at: 954 954
Size: 4532
AccI GT'mk_AC
Cuts at: 1570 1570
Size: 4532
Acc65I G'GTAC_C
Cuts at: 1548 1548
Size: 4532
AclI AA'CG_TT
Cuts at: 4372 4372
Size: 4532
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2357 2789 3536 3804 2357
Size: 432 747 268 3085
Fragments arranged by size:
3085 747 432 268
AfeI AGC'GCT
Cuts at: 1465 1465
Size: 4532
AflII C'TTAA_G
Cuts at: 1381 1381
Size: 4532
AflIII A'CryG_T
Cuts at: 462 1454 1884 2988 3965 462
Size: 992 430 1104 977 1029
Fragments arranged by size:
1104 1029 992 977 430
AgeI A'CCGG_T
Cuts at: 2920 2920
Size: 4532
AleI CACnn'nnGTG
Cuts at: 527 527
Size: 4532
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 898 930 898
Size: 32 4500
AlwNI CAG_nnn'CTG
Cuts at: 1372 1837 3404 1372
Size: 465 1567 2500
Fragments arranged by size:
2500 1567 465
ApaLI G'TGCA_C
Cuts at: 3302 3302
Size: 4532
ApoI r'AATT_y
Cuts at: 210 856 912 1536 3885 4141 210
Size: 646 56 624 2349 256 601
Fragments arranged by size:
2349 646 624 601 256 56
AseI AT'TA_AT
Cuts at: 20 20
Size: 4532
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4532
AvaI C'yCGr_G
Cuts at: 1552 1552
Size: 4532
BamHI G'GATC_C
Cuts at: 1557 1557
Size: 4532
BanI G'GyrC_C
Cuts at: 1548 1962 2210 2245 1548
Size: 414 248 35 3835
Fragments arranged by size:
3835 414 248 35
BanII G_rGCy'C
Cuts at: 1236 1546 2576 1236
Size: 310 1030 3192
Fragments arranged by size:
3192 1030 310
BbeI G_GCGC'C
Cuts at: 1966 2214 1966
Size: 248 4284
BbsI GAAGACnn'nnnn_
Cuts at: 1434 1434
Size: 4532
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 210 244 210
Size: 34 4498
BciVI GTATCCnnnnn_n'
Cuts at: 2430 3191 4532 2430
Size: 761 1341 2430
Fragments arranged by size:
2430 1341 761
BclI T'GATC_A
Cuts at: 1055 2052 1055
Size: 997 3535
BglII A'GATC_T
Cuts at: 2047 4084 2047
Size: 2037 2495
BlpI GC'TnA_GC
Cuts at: 1644 1644
Size: 4532
Bme1580I G_kGCm'C
Cuts at: 2157 2250 3306 2157
Size: 93 1056 3383
Fragments arranged by size:
3383 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 881 1293 1446 1744 1825 2160 4304 881
Size: 412 153 298 81 335 2144 1109
Fragments arranged by size:
2144 1109 412 335 298 153 81
BmtI G_CTAG'C
Cuts at: 961 961
Size: 4532
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2899 2899
Size: 4532
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 226 656 1142 1259 226
Size: 430 486 117 3499
Fragments arranged by size:
3499 486 430 117
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1091 1708 2587 3079 3377 1091
Size: 617 879 492 298 2246
Fragments arranged by size:
2246 879 617 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1093 1296 4236 1093
Size: 203 2940 1389
Fragments arranged by size:
2940 1389 203
BsaAI yAC'GTr
Cuts at: 1457 1524 1885 1457
Size: 67 361 4104
Fragments arranged by size:
4104 361 67
BsaBI GATnn'nnATC
Cuts at: 6 1072 2046 4083 6
Size: 1066 974 2037 455
Fragments arranged by size:
2037 1066 974 455
BsaHI Gr'CG_yC
Cuts at: 951 1963 2211 2913 4409 951
Size: 1012 248 702 1496 1074
Fragments arranged by size:
1496 1074 1012 702 248
BsaWI w'CCGG_w
Cuts at: 317 1721 2242 2920 3194 3341 3752 317
Size: 1404 521 678 274 147 411 1097
Fragments arranged by size:
1404 1097 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 679 709 1140 1170 679
Size: 30 431 30 4041
Fragments arranged by size:
4041 431 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 319 431 700 319
Size: 112 269 4151
Fragments arranged by size:
4151 269 112
BseYI C'CCAG_C
Cuts at: 1957 2715 3292 3788 1957
Size: 758 577 496 2701
Fragments arranged by size:
2701 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2074 2120 3328 104
Size: 1970 46 1208 1308
Fragments arranged by size:
1970 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1236 1546 2324 2514 3306 1236
Size: 310 778 190 792 2462
Fragments arranged by size:
2462 792 778 310 190
Bsp1286I G_dGCh'C
Cuts at: 1236 1546 2157 2250 2324 2514 2576 3306 1236
Size: 310 611 93 74 190 62 730 2462
Fragments arranged by size:
2462 730 611 310 190 93 74 62
BspEI T'CCGG_A
Cuts at: 317 3752 317
Size: 3435 1097
BspHI T'CATG_A
Cuts at: 3708 3708
Size: 4532
BspMI ACCTGCnnnn'nnnn_
Cuts at: 504 1314 1582 1796 2098 2479 504
Size: 810 268 214 302 381 2557
Fragments arranged by size:
2557 810 381 302 268 214
BsrBI CCG'CTC
Cuts at: 50 2824 2878 4362 4527 50
Size: 2774 54 1484 165 55
Fragments arranged by size:
2774 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 137 479 989 2444 137
Size: 342 510 1455 2225
Fragments arranged by size:
2225 1455 510 342
BsrFI r'CCGG_y
Cuts at: 2530 2711 2920 2530
Size: 181 209 4142
Fragments arranged by size:
4142 209 181
BssSI C'ACGA_G
Cuts at: 2803 3161 2803
Size: 358 4174
BstAPI GCAn_nnn'nTGC
Cuts at: 1909 1909
Size: 4532
BstBI TT'CG_AA
Cuts at: 110 2893 110
Size: 2783 1749
BstYI r'GATC_y
Cuts at: 1 1188 1557 2024 2047 2382 2628 3640 3726
Size: 1187 369 467 23 335 246 1012 86
Cuts at: 3726 3738 4084 1
Size: 12 346 449
Fragments arranged by size:
1187 1012 467 449 369 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 998 998
Size: 4532
BtgI C'CryG_G
Cuts at: 125 125
Size: 4532
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2482 2663 2826 2482
Size: 181 163 4188
Fragments arranged by size:
4188 181 163
BtsI GCAGTG_nn'
Cuts at: 1775 1775
Size: 4532
DraI TTT'AAA
Cuts at: 1531 1531
Size: 4532
DrdI GACnn_nn'nnGTC
Cuts at: 2238 3096 2238
Size: 858 3674
EaeI y'GGCC_r
Cuts at: 519 1394 2117 2291 2682 2709 4054 519
Size: 875 723 174 391 27 1345 997
Fragments arranged by size:
1345 997 875 723 391 174 27
EagI C'GGCC_G
Cuts at: 2117 2117
Size: 4532
EarI CTCTTCn'nnn_
Cuts at: 358 677 2555 2765 358
Size: 319 1878 210 2125
Fragments arranged by size:
2125 1878 319 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2740 3050 3196 4342 2740
Size: 310 146 1146 2930
Fragments arranged by size:
2930 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1234 1544 1234
Size: 310 4222
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 226 656 1142 1259 2357 2789 3536 3804 226
Size: 430 486 117 1098 432 747 268 954
Fragments arranged by size:
1098 954 747 486 432 430 268 117
EcoO109I rG'GnC_Cy
Cuts at: 1473 1671 1473
Size: 198 4334
EcoRI G'AATT_C
Cuts at: 912 1536 912
Size: 624 3908
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1280 2551 1280
Size: 1271 3261
FspI TGC'GCA
Cuts at: 2313 2313
Size: 4532
HaeII r_GCGC'y
Cuts at: 1467 1966 2214 3236 4215 1467
Size: 499 248 1022 979 1784
Fragments arranged by size:
1784 1022 979 499 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 379 411 1027 1059 1140 1172 1330 1362 2374
Size: 32 616 32 81 32 158 32 1012
Cuts at: 2374 2404 2406 2436 379
Size: 30 2 30 2475
Fragments arranged by size:
2475 1012 616 158 81 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1281 1571 1281
Size: 290 4242
KasI G'GCGC_C
Cuts at: 1962 2210 1962
Size: 248 4284
KpnI G_GTAC'C
Cuts at: 1552 1552
Size: 4532
MluI A'CGCG_T
Cuts at: 3965 3965
Size: 4532
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3203 3387 3203
Size: 184 4348
MscI TGG'CCA
Cuts at: 521 1396 2293 521
Size: 875 897 2760
Fragments arranged by size:
2760 897 875
MslI CAynn'nnrTG
Cuts at: 130 467 527 547 2521 2648 130
Size: 337 60 20 1974 127 2014
Fragments arranged by size:
2014 1974 337 127 60 20
MspA1I CmG'CkG
Cuts at: 1643 1957 2317 3330 3575 1643
Size: 314 360 1013 245 2600
Fragments arranged by size:
2600 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2713 2713
Size: 4532
NarI GG'CG_CC
Cuts at: 1963 2211 1963
Size: 248 4284
NcoI C'CATG_G
Cuts at: 125 125
Size: 4532
NgoMIV G'CCGG_C
Cuts at: 2711 2711
Size: 4532
NheI G'CTAG_C
Cuts at: 957 957
Size: 4532
NspI r_CATG'y
Cuts at: 334 466 1585 2085 2992 334
Size: 132 1119 500 907 1874
Fragments arranged by size:
1874 1119 907 500 132
PciI A'CATG_T
Cuts at: 462 2988 462
Size: 2526 2006
PfoI T'CCnGG_A
Cuts at: 1236 1236
Size: 4532
PmeI GTTT'AAAC
Cuts at: 1531 1531
Size: 4532
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3671 3703 3671
Size: 32 4500
PshAI GACnn'nnGTC
Cuts at: 818 818
Size: 4532
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 425 457 425
Size: 32 4500
PstI C_TGCA'G
Cuts at: 1579 2264 1579
Size: 685 3847
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4532
PvuII CAG'CTG
Cuts at: 1957 2317 1957
Size: 360 4172
RsrII CG'GwC_CG
Cuts at: 2727 2727
Size: 4532
SacI G_AGCT'C
Cuts at: 1236 1546 1236
Size: 310 4222
SalI G'TCGA_C
Cuts at: 1569 1569
Size: 4532
SapI GCTCTTCn'nnn_
Cuts at: 2555 2765 2555
Size: 210 4322
SbfI CC_TGCA'GG
Cuts at: 1579 1579
Size: 4532
ScaI AGT'ACT
Cuts at: 4049 4049
Size: 4532
SfcI C'TryA_G
Cuts at: 33 585 1575 2260 3253 3444 33
Size: 552 990 685 993 191 1121
Fragments arranged by size:
1121 993 990 685 552 191
SfoI GGC'GCC
Cuts at: 1964 2212 1964
Size: 248 4284
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4532
SmaI CCC'GGG
Cuts at: 1554 1554
Size: 4532
SmlI C'TyrA_G
Cuts at: 1106 1381 1687 2602 3094 3356 1106
Size: 275 306 915 492 262 2282
Fragments arranged by size:
2282 915 492 306 275 262
SnaBI TAC'GTA
Cuts at: 1524 1524
Size: 4532
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4532
SphI G_CATG'C
Cuts at: 1585 2085 1585
Size: 500 4032
StuI AGG'CCT
Cuts at: 293 293
Size: 4532
StyI C'CwwG_G
Cuts at: 125 1184 1666 2020 125
Size: 1059 482 354 2637
Fragments arranged by size:
2637 1059 482 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2115 2248 2707 2916 2115
Size: 133 459 209 3731
Fragments arranged by size:
3731 459 209 133
TatI w'GTAC_w
Cuts at: 4047 4047
Size: 4532
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4049 4081 4049
Size: 32 4500
Tth111I GACn'n_nGTC
Cuts at: 1438 2329 1438
Size: 891 3641
XbaI T'CTAG_A
Cuts at: 1563 1563
Size: 4532
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 201 2027 201
Size: 1826 2706
XmaI C'CCGG_G
Cuts at: 1552 1552
Size: 4532
XmnI GAAnn'nnTTC
Cuts at: 580 580
Size: 4532
ZraI GAC'GTC
Cuts at: 952 952
Size: 4532
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AleI AloI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglII BlpI Bme1580I BmrI BmtI BplI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI Bsu36I BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NheI NspI PciI PfoI
PmeI PpiI PshAI PsrI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SnaBI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AhdI AlfI ApaI AscI AvrII BaeI BbvCI
BfrBI BglI BmgBI Bpu10I BsgI BsiWI BsmI BsmBI
BsrGI BssHII BstEII BstXI BstZ17I ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NotI NruI NsiI PacI PasI PflMI PmlI PpuMI
PsiI PspOMI PspXI SacII SanDI SexAI SfiI SgrAI
SrfI SspI SwaI XhoI