Restriction map for
pF1KSDB1439
(Circular) (Six-base) MAPSORT of: pF1KSDB1439.seq Check: 1687 from: 1 to: 9956
pF1KSDB1439 9956 bp
With 182 enzymes: SgfI *
June 20, 2008 10:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1347 4580 1347
Size: 3233 6723
AatII G_ACGT'C
Cuts at: 4424 4424
Size: 9956
AccI GT'mk_AC
Cuts at: 659 6311 6930 6994 659
Size: 5652 619 64 3621
Fragments arranged by size:
5652 3621 619 64
Acc65I G'GTAC_C
Cuts at: 2690 6972 2690
Size: 4282 5674
AclI AA'CG_TT
Cuts at: 9796 9796
Size: 9956
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 745 756 1378 1571 2191 2515 3460 3586 3967
Size: 11 622 193 620 324 945 126 381
Cuts at: 3967 4359 4480 4979 5138 5912 6170 7781 8213
Size: 392 121 499 159 774 258 1611 432
Cuts at: 8213 8960 9228 745
Size: 747 268 1473
Fragments arranged by size:
1611 1473 945 774 747 622 620 499
432 392 381 324 268 258 193 159
126 121 11
AfeI AGC'GCT
Cuts at: 760 760
Size: 9956
AflIII A'CryG_T
Cuts at: 1392 1760 1790 3685 7308 8412 9389 1392
Size: 368 30 1895 3623 1104 977 1959
Fragments arranged by size:
3623 1959 1895 1104 977 368 30
AgeI A'CCGG_T
Cuts at: 8344 8344
Size: 9956
AhdI GACnn_n'nnGTC
Cuts at: 6887 6887
Size: 9956
AleI CACnn'nnGTG
Cuts at: 5155 6094 5155
Size: 939 9017
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1747 1779 2715 2747 3800 3832 1747
Size: 32 936 32 1053 32 7871
Fragments arranged by size:
7871 1053 936 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 1254 4197 7261 8828 1254
Size: 2943 3064 1567 2382
Fragments arranged by size:
3064 2943 2382 1567
ApaI G_GGCC'C
Cuts at: 1433 1582 4903 1433
Size: 149 3321 6486
Fragments arranged by size:
6486 3321 149
ApaLI G'TGCA_C
Cuts at: 8726 8726
Size: 9956
ApoI r'AATT_y
Cuts at: 974 2623 3914 4685 4771 4802 6604 6864 6960
Size: 1649 1291 771 86 31 1802 260 96
Cuts at: 6960 9309 9565 974
Size: 2349 256 1365
Fragments arranged by size:
2349 1802 1649 1365 1291 771 260 256
96 86 31
AseI AT'TA_AT
Cuts at: 20 20
Size: 9956
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 9956
AvaI C'yCGr_G
Cuts at: 1105 1121 1583 3411 4633 5071 6911 6976 1105
Size: 16 462 1828 1222 438 1840 65 4085
Fragments arranged by size:
4085 1840 1828 1222 462 438 65 16
BamHI G'GATC_C
Cuts at: 1194 2841 6981 1194
Size: 1647 4140 4169
Fragments arranged by size:
4169 4140 1647
BanI G'GyrC_C
Cuts at: 829 2690 3550 4095 4639 5971 6049 6562 6580
Size: 1861 860 545 544 1332 78 513 18
Cuts at: 6580 6972 7386 7634 7669 829
Size: 392 414 248 35 3116
Fragments arranged by size:
3116 1861 1332 860 545 544 513 414
392 248 78 35 18
BanII G_rGCy'C
Cuts at: 1433 1582 2805 2822 2945 4903 5071 5284 5852
Size: 149 1223 17 123 1958 168 213 568
Cuts at: 5852 5864 6416 6970 8000 1433
Size: 12 552 554 1030 3389
Fragments arranged by size:
3389 1958 1223 1030 568 554 552 213
168 149 123 17 12
BbeI G_GCGC'C
Cuts at: 4099 6566 7390 7638 4099
Size: 2467 824 248 6417
Fragments arranged by size:
6417 2467 824 248
BbsI GAAGACnn'nnnn_
Cuts at: 854 1154 4516 4967 5622 854
Size: 300 3362 451 655 5188
Fragments arranged by size:
5188 3362 655 451 300
BbvCI CC'TCA_GC
Cuts at: 312 5611 312
Size: 5299 4657
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 175 209 4013 4047 5346 5380 6256 6290 175
Size: 34 3804 34 1299 34 876 34 3841
Fragments arranged by size:
3841 3804 1299 876 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 1346 1805 4748 7854 8615 9956 1346
Size: 459 2943 3106 761 1341 1346
Fragments arranged by size:
3106 2943 1346 1341 761 459
BclI T'GATC_A
Cuts at: 668 1475 2069 2697 7476 668
Size: 807 594 628 4779 3148
Fragments arranged by size:
4779 3148 807 628 594
BfrBI ATG'CAT
Cuts at: 1240 2286 1240
Size: 1046 8910
BglI GCCn_nnn'nGGC
Cuts at: 2404 5416 5503 6329 2404
Size: 3012 87 826 6031
Fragments arranged by size:
6031 3012 826 87
BglII A'GATC_T
Cuts at: 937 1436 7471 9508 937
Size: 499 6035 2037 1385
Fragments arranged by size:
6035 2037 1385 499
BlpI GC'TnA_GC
Cuts at: 2377 2489 3188 3995 7068 2377
Size: 112 699 807 3073 5265
Fragments arranged by size:
5265 3073 807 699 112
Bme1580I G_kGCm'C
Cuts at: 832 1433 1582 1740 4144 4633 4903 5974 6583
Size: 601 149 158 2404 489 270 1071 609
Cuts at: 6583 7581 7674 8730 832
Size: 998 93 1056 2058
Fragments arranged by size:
2404 2058 1071 1056 998 609 601 489
270 158 149 93
BmgBI CAC'GTC
Cuts at: 1854 3699 1854
Size: 1845 8111
BmrI ACTGGGnnnn_n'
Cuts at: 4819 5304 5748 6092 7168 7249 7584 9728 4819
Size: 485 444 344 1076 81 335 2144 5047
Fragments arranged by size:
5047 2144 1076 485 444 344 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4837 5145 5736 6843 8323 4837
Size: 308 591 1107 1480 6470
Fragments arranged by size:
6470 1480 1107 591 308
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 319 775 1276 1641 1696 1771 1994 2011 3265
Size: 456 501 365 55 75 223 17 1254
Cuts at: 3265 3698 3895 4165 5245 5378 5961 6038 6303
Size: 433 197 270 1080 133 583 77 265
Cuts at: 6303 6727 6941 319
Size: 424 214 3334
Fragments arranged by size:
3334 1254 1080 583 501 456 433 424
365 270 265 223 214 197 133 77
75 55 17
Bpu10I CC'TnA_GC
Cuts at: 312 3302 3450 3546 5593 5611 312
Size: 2990 148 96 2047 18 4657
Fragments arranged by size:
4657 2990 2047 148 96 18
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 662 905 5958 6580 7132 8011 8503 8801 662
Size: 243 5053 622 552 879 492 298 1817
Fragments arranged by size:
5053 1817 879 622 552 492 298 243
BsaI GGTCTCn'nnnn_
Cuts at: 1048 2170 3904 6759 9660 1048
Size: 1122 1734 2855 2901 1344
Fragments arranged by size:
2901 2855 1734 1344 1122
BsaAI yAC'GTr
Cuts at: 1824 3686 6335 6948 7309 1824
Size: 1862 2649 613 361 4471
Fragments arranged by size:
4471 2649 1862 613 361
BsaBI GATnn'nnATC
Cuts at: 6 1075 2257 2278 7470 9507 6
Size: 1069 1182 21 5192 2037 455
Fragments arranged by size:
5192 2037 1182 1069 455 21
BsaHI Gr'CG_yC
Cuts at: 4096 4421 6563 7387 7635 8337 9833 4096
Size: 325 2142 824 248 702 1496 4219
Fragments arranged by size:
4219 2142 1496 824 702 325 248
BsaWI w'CCGG_w
Cuts at: 542 1928 2520 4476 5582 7145 7666 8344 8618
Size: 1386 592 1956 1106 1563 521 678 274
Cuts at: 8618 8765 9176 542
Size: 147 411 1322
Fragments arranged by size:
1956 1563 1386 1322 1106 678 592 521
411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2110 2140 2146 2176 2224 2254 2110
Size: 30 6 30 48 30 9812
Fragments arranged by size:
9812 48 30 30 30 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 451 3247 3892 5089 5728 6747 6750 6753 6778
Size: 2796 645 1197 639 1019 3 3 25
Cuts at: 6778 6781 6835 451
Size: 3 54 3572
Fragments arranged by size:
3572 2796 1197 1019 645 639 54 25
3 3 3
BseYI C'CCAG_C
Cuts at: 3025 3209 3239 4538 5205 5341 5463 5488 5982
Size: 184 30 1299 667 136 122 25 494
Cuts at: 5982 7381 8139 8716 9212 3025
Size: 1399 758 577 496 3769
Fragments arranged by size:
3769 1399 1299 758 667 577 496 494
184 136 122 30 25
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2017 4210 4614 6736 6889 2017
Size: 2193 404 2122 153 5084
Fragments arranged by size:
5084 2193 2122 404 153
BsiEI CG_ry'CG
Cuts at: 104 997 7498 7544 8752 104
Size: 893 6501 46 1208 1308
Fragments arranged by size:
6501 1308 1208 893 46
BsiHKAI G_wGCw'C
Cuts at: 817 1130 2852 3089 3194 4262 5720 6970 7748
Size: 313 1722 237 105 1068 1458 1250 778
Cuts at: 7748 7938 8730 817
Size: 190 792 2043
Fragments arranged by size:
2043 1722 1458 1250 1068 792 778 313
237 190 105
BsmI GAATG_Cn'
Cuts at: 261 289 326 474 1368 3004 261
Size: 28 37 148 894 1636 7213
Fragments arranged by size:
7213 1636 894 148 37 28
BsmBI CGTCTCn'nnnn_
Cuts at: 774 3408 3874 774
Size: 2634 466 6856
Fragments arranged by size:
6856 2634 466
Bsp1286I G_dGCh'C
Cuts at: 817 832 1130 1433 1582 1740 2805 2822 2852
Size: 15 298 303 149 158 1065 17 30
Cuts at: 2852 2945 3089 3194 4144 4262 4633 4903 5071
Size: 93 144 105 950 118 371 270 168
Cuts at: 5071 5284 5720 5852 5864 5974 6416 6583 6970
Size: 213 436 132 12 110 442 167 387
Cuts at: 6970 7581 7674 7748 7938 8000 8730 817
Size: 611 93 74 190 62 730 2043
Fragments arranged by size:
2043 1065 950 730 611 442 436 387
371 303 298 270 213 190 168 167
158 149 144 132 118 110 105 93
93 74 62 30 17 15 12
BspEI T'CCGG_A
Cuts at: 542 4476 9176 542
Size: 3934 4700 1322
Fragments arranged by size:
4700 3934 1322
BspHI T'CATG_A
Cuts at: 279 464 2066 4883 5321 6891 9132 279
Size: 185 1602 2817 438 1570 2241 1103
Fragments arranged by size:
2817 2241 1602 1570 1103 438 185
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1347 2453 3661 4580 6665 7006 7220 7522 7903
Size: 1106 1208 919 2085 341 214 302 381
Cuts at: 7903 1347
Size: 3400
Fragments arranged by size:
3400 2085 1208 1106 919 381 341 302
214
BsrBI CCG'CTC
Cuts at: 50 267 4895 5238 8248 8302 9786 9951 50
Size: 217 4628 343 3010 54 1484 165 55
Fragments arranged by size:
4628 3010 1484 343 217 165 55 54
BsrDI GCAATG_nn'
Cuts at: 357 3344 3512 3521 7868 357
Size: 2987 168 9 4347 2445
Fragments arranged by size:
4347 2987 2445 168 9
BsrFI r'CCGG_y
Cuts at: 4098 6052 7954 8135 8344 4098
Size: 1954 1902 181 209 5710
Fragments arranged by size:
5710 1954 1902 209 181
BsrGI T'GTAC_A
Cuts at: 1322 1322
Size: 9956
BssSI C'ACGA_G
Cuts at: 211 8227 8585 211
Size: 8016 358 1582
Fragments arranged by size:
8016 1582 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1011 2652 4197 4257 4454 7333 1011
Size: 1641 1545 60 197 2879 3634
Fragments arranged by size:
3634 2879 1641 1545 197 60
BstBI TT'CG_AA
Cuts at: 110 8317 110
Size: 8207 1749
BstEII G'GTnAC_C
Cuts at: 1020 5298 1020
Size: 4278 5678
BstXI CCAn_nnnn'nTGG
Cuts at: 490 2735 5157 5775 6426 490
Size: 2245 2422 618 651 4020
Fragments arranged by size:
4020 2422 2245 651 618
BstYI r'GATC_y
Cuts at: 1 341 937 1194 1436 1835 2114 2841 3429
Size: 340 596 257 242 399 279 727 588
Cuts at: 3429 6776 6981 7448 7471 7806 8052 9064 9150
Size: 3347 205 467 23 335 246 1012 86
Cuts at: 9150 9162 9508 1
Size: 12 346 449
Fragments arranged by size:
3347 1012 727 596 588 467 449 399
346 340 335 279 257 246 242 205
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 5057 6102 5057
Size: 1045 8911
BtgI C'CryG_G
Cuts at: 125 328 1916 2609 5768 125
Size: 203 1588 693 3159 4313
Fragments arranged by size:
4313 3159 1588 693 203
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 319 717 6357 7906 8087 8250 319
Size: 398 5640 1549 181 163 2025
Fragments arranged by size:
5640 2025 1549 398 181 163
BtsI GCAGTG_nn'
Cuts at: 2213 2348 2590 3648 4401 5419 5550 5993 6717
Size: 135 242 1058 753 1018 131 443 724
Cuts at: 6717 7199 2213
Size: 482 4970
Fragments arranged by size:
4970 1058 1018 753 724 482 443 242
135 131
ClaI AT'CG_AT
Cuts at: 6194 6194
Size: 9956
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 3770 3805 3770
Size: 35 9921
DraI TTT'AAA
Cuts at: 6955 6955
Size: 9956
DraIII CAC_nnn'GTG
Cuts at: 3686 3686
Size: 9956
DrdI GACnn_nn'nnGTC
Cuts at: 655 4078 7662 8520 655
Size: 3423 3584 858 2091
Fragments arranged by size:
3584 3423 2091 858
EaeI y'GGCC_r
Cuts at: 492 994 1607 2949 4875 6545 7541 7715 8106
Size: 502 613 1342 1926 1670 996 174 391
Cuts at: 8106 8133 9478 492
Size: 27 1345 970
Fragments arranged by size:
1926 1670 1345 1342 996 970 613 502
391 174 27
EagI C'GGCC_G
Cuts at: 994 7541 994
Size: 6547 3409
EarI CTCTTCn'nnn_
Cuts at: 564 889 2134 2329 2558 3290 3565 3929 7979
Size: 325 1245 195 229 732 275 364 4050
Cuts at: 7979 8189 564
Size: 210 2331
Fragments arranged by size:
4050 2331 1245 732 364 325 275 229
210 195
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1645 2017 8164 8474 8620 9766 1645
Size: 372 6147 310 146 1146 1835
Fragments arranged by size:
6147 1835 1146 372 310 146
EcoICRI GAG'CTC
Cuts at: 6968 6968
Size: 9956
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 319 745 756 775 1276 1378 1571 1641 1696
Size: 426 11 19 501 102 193 70 55
Cuts at: 1696 1771 1994 2011 2191 2515 3265 3460 3586
Size: 75 223 17 180 324 750 195 126
Cuts at: 3586 3698 3895 3967 4165 4359 4480 4979 5138
Size: 112 197 72 198 194 121 499 159
Cuts at: 5138 5245 5378 5912 5961 6038 6170 6303 6727
Size: 107 133 534 49 77 132 133 424
Cuts at: 6727 6941 7781 8213 8960 9228 319
Size: 214 840 432 747 268 1047
Fragments arranged by size:
1047 840 750 747 534 501 499 432
426 424 324 268 223 214 198 197
195 194 193 180 159 133 133 132
126 121 112 107 102 77 75 72
70 55 49 19 17 11
EcoNI CCTnn'n_nnAGG
Cuts at: 823 1573 2698 2827 5056 5116 823
Size: 750 1125 129 2229 60 5663
Fragments arranged by size:
5663 2229 1125 750 129 60
EcoO109I rG'GnC_Cy
Cuts at: 747 1028 1429 1430 1578 1579 3306 3650 3973
Size: 281 401 1 148 1 1727 344 323
Cuts at: 3973 4039 4647 4899 4900 5655 5742 7095 747
Size: 66 608 252 1 755 87 1353 3608
Fragments arranged by size:
3608 1727 1353 755 608 401 344 323
281 252 148 87 66 1 1 1
EcoRI G'AATT_C
Cuts at: 974 2623 6960 974
Size: 1649 4337 3970
Fragments arranged by size:
4337 3970 1649
EcoRV GAT'ATC
Cuts at: 591 591
Size: 9956
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 213 2196 3591 4458 4489 6582 7975 213
Size: 1983 1395 867 31 2093 1393 2194
Fragments arranged by size:
2194 2093 1983 1395 1393 867 31
FspI TGC'GCA
Cuts at: 3607 7737 3607
Size: 4130 5826
HaeII r_GCGC'y
Cuts at: 724 762 3995 4099 4198 5820 6566 7390 7638
Size: 38 3233 104 99 1622 746 824 248
Cuts at: 7638 8660 9639 724
Size: 1022 979 1041
Fragments arranged by size:
3233 1622 1041 1022 979 824 746 248
104 99 38
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 660 692 1794 1826 2224 2256 3394 3407 3426
Size: 32 1102 32 398 32 1138 13 19
Cuts at: 3426 3439 3998 4030 5429 5461 6873 6905 7798
Size: 13 559 32 1399 32 1412 32 893
Cuts at: 7798 7828 7830 7860 660
Size: 30 2 30 2756
Fragments arranged by size:
2756 1412 1399 1138 1102 893 559 398
32 32 32 32 32 32 30 30
19 13 13 2
HincII GTy'rAC
Cuts at: 4017 4572 6931 6995 4017
Size: 555 2359 64 6978
Fragments arranged by size:
6978 2359 555 64
KasI G'GCGC_C
Cuts at: 4095 6562 7386 7634 4095
Size: 2467 824 248 6417
Fragments arranged by size:
6417 2467 824 248
KpnI G_GTAC'C
Cuts at: 2694 6976 2694
Size: 4282 5674
MluI A'CGCG_T
Cuts at: 9389 9389
Size: 9956
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1995 3242 4239 4742 5985 8627 8811 1995
Size: 1247 997 503 1243 2642 184 3140
Fragments arranged by size:
3140 2642 1247 1243 997 503 184
MscI TGG'CCA
Cuts at: 1609 2951 6547 7717 1609
Size: 1342 3596 1170 3848
Fragments arranged by size:
3848 3596 1342 1170
MslI CAynn'nnrTG
Cuts at: 1465 1852 2134 2251 2359 2647 3466 3701 4888
Size: 387 282 117 108 288 819 235 1187
Cuts at: 4888 4972 5155 5179 5452 5773 6094 6424 7945
Size: 84 183 24 273 321 321 330 1521
Cuts at: 7945 8072 1465
Size: 127 3349
Fragments arranged by size:
3349 1521 1187 819 387 330 321 321
288 282 273 235 183 127 117 108
84 24
MspA1I CmG'CkG
Cuts at: 2084 2488 2959 3213 3243 3659 4173 4454 4542
Size: 404 471 254 30 416 514 281 88
Cuts at: 4542 6396 7067 7381 7741 8754 8999 2084
Size: 1854 671 314 360 1013 245 3041
Fragments arranged by size:
3041 1854 1013 671 514 471 416 404
360 314 281 254 245 88 30
NaeI GCC'GGC
Cuts at: 4100 6054 8137 4100
Size: 1954 2083 5919
Fragments arranged by size:
5919 2083 1954
NarI GG'CG_CC
Cuts at: 4096 6563 7387 7635 4096
Size: 2467 824 248 6417
Fragments arranged by size:
6417 2467 824 248
NcoI C'CATG_G
Cuts at: 125 5768 125
Size: 5643 4313
NdeI CA'TA_TG
Cuts at: 3852 3852
Size: 9956
NgoMIV G'CCGG_C
Cuts at: 4098 6052 8135 4098
Size: 1954 2083 5919
Fragments arranged by size:
5919 2083 1954
NsiI A_TGCA'T
Cuts at: 1242 2288 1242
Size: 1046 8910
NspI r_CATG'y
Cuts at: 1764 1794 3147 3198 3324 4189 5002 6450 6741
Size: 30 1353 51 126 865 813 1448 291
Cuts at: 6741 7009 7509 8416 1764
Size: 268 500 907 3304
Fragments arranged by size:
3304 1448 1353 907 865 813 500 291
268 126 51 30
PasI CC'CwG_GG
Cuts at: 825 1488 3528 4090 825
Size: 663 2040 562 6691
Fragments arranged by size:
6691 2040 663 562
PciI A'CATG_T
Cuts at: 1760 1790 8412 1760
Size: 30 6622 3304
Fragments arranged by size:
6622 3304 30
PflMI CCAn_nnn'nTGG
Cuts at: 531 1254 3076 4444 5294 6513 531
Size: 723 1822 1368 850 1219 3974
Fragments arranged by size:
3974 1822 1368 1219 850 723
PfoI T'CCnGG_A
Cuts at: 396 710 752 1688 2228 2709 3725 5231 396
Size: 314 42 936 540 481 1016 1506 5121
Fragments arranged by size:
5121 1506 1016 936 540 481 314 42
PmeI GTTT'AAAC
Cuts at: 6955 6955
Size: 9956
PmlI CAC'GTG
Cuts at: 1824 3686 1824
Size: 1862 8094
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 9095 9127 9095
Size: 32 9924
PpuMI rG'GwC_Cy
Cuts at: 3306 3650 3973 4039 5742 3306
Size: 344 323 66 1703 7520
Fragments arranged by size:
7520 1703 344 323 66
PshAI GACnn'nnGTC
Cuts at: 3896 4567 5692 3896
Size: 671 1125 8160
Fragments arranged by size:
8160 1125 671
PsiI TTA'TAA
Cuts at: 4750 4750
Size: 9956
PspOMI G'GGCC_C
Cuts at: 1429 1578 4899 1429
Size: 149 3321 6486
Fragments arranged by size:
6486 3321 149
PspXI vC'TCGA_Gb
Cuts at: 6911 6911
Size: 9956
PstI C_TGCA'G
Cuts at: 742 1344 1961 2585 3658 4591 7003 7688 742
Size: 602 617 624 1073 933 2412 685 3010
Fragments arranged by size:
3010 2412 1073 933 685 624 617 602
PvuI CG_AT'CG
Cuts at: 104 104
Size: 9956
PvuII CAG'CTG
Cuts at: 2959 3213 3243 3659 4173 4542 7381 7741 2959
Size: 254 30 416 514 369 2839 360 5174
Fragments arranged by size:
5174 2839 514 416 369 360 254 30
RsrII CG'GwC_CG
Cuts at: 8151 8151
Size: 9956
SacI G_AGCT'C
Cuts at: 6970 6970
Size: 9956
SalI G'TCGA_C
Cuts at: 6929 6993 6929
Size: 64 9892
SanDI GG'GwC_CC
Cuts at: 4039 4039
Size: 9956
SapI GCTCTTCn'nnn_
Cuts at: 889 2134 2558 7979 8189 889
Size: 1245 424 5421 210 2656
Fragments arranged by size:
5421 2656 1245 424 210
SbfI CC_TGCA'GG
Cuts at: 4591 7003 4591
Size: 2412 7544
ScaI AGT'ACT
Cuts at: 6826 9473 6826
Size: 2647 7309
SexAI A'CCwGG_T
Cuts at: 2221 2549 6851 2221
Size: 328 4302 5326
Fragments arranged by size:
5326 4302 328
SfcI C'TryA_G
Cuts at: 33 270 738 835 1340 1957 2415 2581 3654
Size: 237 468 97 505 617 458 166 1073
Cuts at: 3654 4587 6999 7684 8677 8868 33
Size: 933 2412 685 993 191 1121
Fragments arranged by size:
2412 1121 1073 993 933 685 617 505
468 458 237 191 166 97
SfoI GGC'GCC
Cuts at: 4097 6564 7388 7636 4097
Size: 2467 824 248 6417
Fragments arranged by size:
6417 2467 824 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 9956
SmaI CCC'GGG
Cuts at: 6978 6978
Size: 9956
SmlI C'TyrA_G
Cuts at: 677 920 3411 5937 6595 6911 7111 8026 8518
Size: 243 2491 2526 658 316 200 915 492
Cuts at: 8518 8780 677
Size: 262 1853
Fragments arranged by size:
2526 2491 1853 915 658 492 316 262
243 200
SnaBI TAC'GTA
Cuts at: 6948 6948
Size: 9956
SpeI A'CTAG_T
Cuts at: 67 67
Size: 9956
SphI G_CATG'C
Cuts at: 3324 5002 6450 7009 7509 3324
Size: 1678 1448 559 500 5771
Fragments arranged by size:
5771 1678 1448 559 500
SspI AAT'ATT
Cuts at: 953 6144 953
Size: 5191 4765
StuI AGG'CCT
Cuts at: 1165 2573 3275 4918 1165
Size: 1408 702 1643 6203
Fragments arranged by size:
6203 1643 1408 702
StyI C'CwwG_G
Cuts at: 125 489 2393 2734 3138 3365 3501 3599 3894
Size: 364 1904 341 404 227 136 98 295
Cuts at: 3894 4872 5117 5768 7090 7444 125
Size: 978 245 651 1322 354 2637
Fragments arranged by size:
2637 1904 1322 978 651 404 364 354
341 295 245 227 136 98
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1008 1744 2830 2867 4124 4552 5286 7539 7672
Size: 736 1086 37 1257 428 734 2253 133
Cuts at: 7672 8131 8340 1008
Size: 459 209 2624
Fragments arranged by size:
2624 2253 1257 1086 736 734 459 428
209 133 37
TatI w'GTAC_w
Cuts at: 1322 3590 4364 5789 6824 9471 1322
Size: 2268 774 1425 1035 2647 1807
Fragments arranged by size:
2647 2268 1807 1425 1035 774
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 199 231 505 537 1000 1032 3627 3659 3672
Size: 32 274 32 463 32 2595 32 13
Cuts at: 3672 3704 4160 4192 5983 6015 9473 9505 199
Size: 32 456 32 1791 32 3458 32 650
Fragments arranged by size:
3458 2595 1791 650 463 456 274 32
32 32 32 32 32 32 32 13
Tth111I GACn'n_nGTC
Cuts at: 7753 7753
Size: 9956
XbaI T'CTAG_A
Cuts at: 4950 6987 4950
Size: 2037 7919
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2400 3022 3351 3396 3480 5269 5985 6423 7451
Size: 622 329 45 84 1789 716 438 1028
Cuts at: 7451 2400
Size: 4905
Fragments arranged by size:
4905 1789 1028 716 622 438 329 84
45
XhoI C'TCGA_G
Cuts at: 3411 6911 3411
Size: 3500 6456
XmaI C'CCGG_G
Cuts at: 6976 6976
Size: 9956
XmnI GAAnn'nnTTC
Cuts at: 5402 5402
Size: 9956
ZraI GAC'GTC
Cuts at: 4422 4422
Size: 9956
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AhdI AleI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NsiI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PstI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AflII AlfI AscI AvrII BaeI BmtI BsiWI BssHII
BstZ17I FseI FspAI HindIII HpaI MfeI NheI NotI
NruI PacI PsrI SacII SfiI SgrAI SrfI SwaI