Restriction map for
pF1KSDB1426
(Circular) (Six-base) MAPSORT of: pF1KSDB1426.seq Check: 471 from: 1 to: 5180
pF1KSDB1426 5180 bp
With 182 enzymes: SgfI *
June 20, 2008 10:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2173 2173
Size: 5180
AatII G_ACGT'C
Cuts at: 1790 1790
Size: 5180
AccI GT'mk_AC
Cuts at: 2218 2218
Size: 5180
Acc65I G'GTAC_C
Cuts at: 2196 2196
Size: 5180
AclI AA'CG_TT
Cuts at: 5020 5020
Size: 5180
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 913 1062 1856 1914 3005 3437 4184 4452 913
Size: 149 794 58 1091 432 747 268 1641
Fragments arranged by size:
1641 1091 794 747 432 268 149 58
AfeI AGC'GCT
Cuts at: 697 697
Size: 5180
AflIII A'CryG_T
Cuts at: 506 2532 3636 4613 506
Size: 2026 1104 977 1073
Fragments arranged by size:
2026 1104 1073 977
AgeI A'CCGG_T
Cuts at: 3568 3568
Size: 5180
AleI CACnn'nnGTG
Cuts at: 307 1867 307
Size: 1560 3620
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 367 728 1483 367
Size: 361 755 4064
Fragments arranged by size:
4064 755 361
AlwNI CAG_nnn'CTG
Cuts at: 465 663 1665 2485 4052 465
Size: 198 1002 820 1567 1593
Fragments arranged by size:
1593 1567 1002 820 198
ApaI G_GGCC'C
Cuts at: 670 670
Size: 5180
ApaLI G'TGCA_C
Cuts at: 3950 3950
Size: 5180
ApoI r'AATT_y
Cuts at: 2184 4533 4789 2184
Size: 2349 256 2575
Fragments arranged by size:
2575 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5180
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5180
AvaI C'yCGr_G
Cuts at: 372 607 878 1397 1410 2200 372
Size: 235 271 519 13 790 3352
Fragments arranged by size:
3352 790 519 271 235 13
BamHI G'GATC_C
Cuts at: 1174 2205 1174
Size: 1031 4149
BanI G'GyrC_C
Cuts at: 269 685 712 757 1249 2127 2196 2610 2858
Size: 416 27 45 492 878 69 414 248
Cuts at: 2858 2893 269
Size: 35 2556
Fragments arranged by size:
2556 878 492 416 414 248 69 45
35 27
BanII G_rGCy'C
Cuts at: 523 622 670 730 832 1375 1590 2081 2194
Size: 99 48 60 102 543 215 491 113
Cuts at: 2194 3224 523
Size: 1030 2479
Fragments arranged by size:
2479 1030 543 491 215 113 102 99
60 48
BbeI G_GCGC'C
Cuts at: 273 2614 2862 273
Size: 2341 248 2591
Fragments arranged by size:
2591 2341 248
BbsI GAAGACnn'nnnn_
Cuts at: 334 1154 334
Size: 820 4360
BbvCI CC'TCA_GC
Cuts at: 559 559
Size: 5180
BciVI GTATCCnnnnn_n'
Cuts at: 1092 3078 3839 5180 1092
Size: 1986 761 1341 1092
Fragments arranged by size:
1986 1341 1092 761
BclI T'GATC_A
Cuts at: 1487 2700 1487
Size: 1213 3967
BglI GCCn_nnn'nGGC
Cuts at: 639 827 639
Size: 188 4992
BglII A'GATC_T
Cuts at: 158 2695 4732 158
Size: 2537 2037 606
Fragments arranged by size:
2537 2037 606
BlpI GC'TnA_GC
Cuts at: 831 2292 831
Size: 1461 3719
Bme1580I G_kGCm'C
Cuts at: 459 577 670 760 1254 2132 2805 2898 3954
Size: 118 93 90 494 878 673 93 1056
Cuts at: 3954 459
Size: 1685
Fragments arranged by size:
1685 1056 878 673 494 118 93 93
90
BmrI ACTGGGnnnn_n'
Cuts at: 498 519 1038 1936 2005 2392 2473 2808 4952
Size: 21 519 898 69 387 81 335 2144
Cuts at: 4952 498
Size: 726
Fragments arranged by size:
2144 898 726 519 387 335 81 69
21
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1425 1606 3547 1425
Size: 181 1941 3058
Fragments arranged by size:
3058 1941 181
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 301 499 1830 2050 301
Size: 198 1331 220 3431
Fragments arranged by size:
3431 1331 220 198
Bpu10I CC'TnA_GC
Cuts at: 559 1306 1682 559
Size: 747 376 4057
Fragments arranged by size:
4057 747 376
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2356 3235 3727 4025 2356
Size: 879 492 298 3511
Fragments arranged by size:
3511 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1886 2010 4884 1886
Size: 124 2874 2182
Fragments arranged by size:
2874 2182 124
BsaAI yAC'GTr
Cuts at: 1356 2172 2533 1356
Size: 816 361 4003
Fragments arranged by size:
4003 816 361
BsaBI GATnn'nnATC
Cuts at: 6 2694 4731 6
Size: 2688 2037 455
Fragments arranged by size:
2688 2037 455
BsaHI Gr'CG_yC
Cuts at: 270 1787 2611 2859 3561 5057 270
Size: 1517 824 248 702 1496 393
Fragments arranged by size:
1517 1496 824 702 393 248
BsaWI w'CCGG_w
Cuts at: 2369 2890 3568 3842 3989 4400 2369
Size: 521 678 274 147 411 3149
Fragments arranged by size:
3149 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 927 957 1588 1618 1909 1939 1990 2020 927
Size: 30 631 30 291 30 51 30 4087
Fragments arranged by size:
4087 631 291 51 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 814 981 1564 1598 1639 814
Size: 167 583 34 41 4355
Fragments arranged by size:
4355 583 167 41 34
BseYI C'CCAG_C
Cuts at: 474 708 1167 2605 3363 3940 4436 474
Size: 234 459 1438 758 577 496 1218
Fragments arranged by size:
1438 1218 758 577 496 459 234
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 331 331
Size: 5180
BsiEI CG_ry'CG
Cuts at: 104 1003 2722 2768 3976 104
Size: 899 1719 46 1208 1308
Fragments arranged by size:
1719 1308 1208 899 46
BsiHKAI G_wGCw'C
Cuts at: 488 730 1565 1773 2081 2194 2972 3162 3954
Size: 242 835 208 308 113 778 190 792
Cuts at: 3954 488
Size: 1714
Fragments arranged by size:
1714 835 792 778 308 242 208 190
113
BsmBI CGTCTCn'nnnn_
Cuts at: 1608 1608
Size: 5180
Bsp1286I G_dGCh'C
Cuts at: 459 488 523 577 622 670 730 760 832
Size: 29 35 54 45 48 60 30 72
Cuts at: 832 1254 1375 1565 1590 1773 2081 2132 2194
Size: 422 121 190 25 183 308 51 62
Cuts at: 2194 2805 2898 2972 3162 3224 3954 459
Size: 611 93 74 190 62 730 1685
Fragments arranged by size:
1685 730 611 422 308 190 190 183
121 93 74 72 62 62 60 54
51 48 45 35 30 29 25
BspEI T'CCGG_A
Cuts at: 4400 4400
Size: 5180
BspHI T'CATG_A
Cuts at: 4356 4356
Size: 5180
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1229 1682 2173 2230 2444 2746 3127 1229
Size: 453 491 57 214 302 381 3282
Fragments arranged by size:
3282 491 453 381 302 214 57
BsrBI CCG'CTC
Cuts at: 50 2034 3472 3526 5010 5175 50
Size: 1984 1438 54 1484 165 55
Fragments arranged by size:
1984 1484 1438 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3092 3092
Size: 5180
BsrFI r'CCGG_y
Cuts at: 446 1065 1157 1378 3178 3359 3568 446
Size: 619 92 221 1800 181 209 2058
Fragments arranged by size:
2058 1800 619 221 209 181 92
BsrGI T'GTAC_A
Cuts at: 1526 1526
Size: 5180
BssSI C'ACGA_G
Cuts at: 3451 3809 3451
Size: 358 4822
BstAPI GCAn_nnn'nTGC
Cuts at: 1388 2557 1388
Size: 1169 4011
BstBI TT'CG_AA
Cuts at: 110 3541 110
Size: 3431 1749
BstEII G'GTnAC_C
Cuts at: 1327 1327
Size: 5180
BstXI CCAn_nnnn'nTGG
Cuts at: 282 642 1869 282
Size: 360 1227 3593
Fragments arranged by size:
3593 1227 360
BstYI r'GATC_y
Cuts at: 1 158 1174 2205 2672 2695 3030 3276 4288
Size: 157 1016 1031 467 23 335 246 1012
Cuts at: 4288 4374 4386 4732 1
Size: 86 12 346 449
Fragments arranged by size:
1031 1016 1012 467 449 346 335 246
157 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1444 1444
Size: 5180
BtgI C'CryG_G
Cuts at: 281 546 601 622 752 1235 281
Size: 265 55 21 130 483 4226
Fragments arranged by size:
4226 483 265 130 55 21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 364 3130 3311 3474 364
Size: 2766 181 163 2070
Fragments arranged by size:
2766 2070 181 163
BtsI GCAGTG_nn'
Cuts at: 262 926 2423 262
Size: 664 1497 3019
Fragments arranged by size:
3019 1497 664
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1013 1048 1013
Size: 35 5145
DraI TTT'AAA
Cuts at: 2179 2179
Size: 5180
DraIII CAC_nnn'GTG
Cuts at: 1643 1643
Size: 5180
DrdI GACnn_nn'nnGTC
Cuts at: 2886 3744 2886
Size: 858 4322
EaeI y'GGCC_r
Cuts at: 429 808 1034 1136 1424 2765 2939 3330 3357
Size: 379 226 102 288 1341 174 391 27
Cuts at: 3357 4702 429
Size: 1345 907
Fragments arranged by size:
1345 1341 907 391 379 288 226 174
102 27
EagI C'GGCC_G
Cuts at: 2765 2765
Size: 5180
EarI CTCTTCn'nnn_
Cuts at: 1477 1951 3203 3413 1477
Size: 474 1252 210 3244
Fragments arranged by size:
3244 1252 474 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3388 3698 3844 4990 3388
Size: 310 146 1146 3578
Fragments arranged by size:
3578 1146 310 146
EcoICRI GAG'CTC
Cuts at: 728 2079 2192 728
Size: 1351 113 3716
Fragments arranged by size:
3716 1351 113
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 301 499 913 1062 1830 1856 1914 2050 3005
Size: 198 414 149 768 26 58 136 955
Cuts at: 3005 3437 4184 4452 301
Size: 432 747 268 1029
Fragments arranged by size:
1029 955 768 747 432 414 268 198
149 136 58 26
EcoNI CCTnn'n_nnAGG
Cuts at: 1225 1225
Size: 5180
EcoO109I rG'GnC_Cy
Cuts at: 627 667 964 1073 1230 1662 2049 2319 627
Size: 40 297 109 157 432 387 270 3488
Fragments arranged by size:
3488 432 387 297 270 157 109 40
EcoRI G'AATT_C
Cuts at: 2184 2184
Size: 5180
EcoRV GAT'ATC
Cuts at: 1875 1875
Size: 5180
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3199 3199
Size: 5180
FspI TGC'GCA
Cuts at: 2961 2961
Size: 5180
HaeII r_GCGC'y
Cuts at: 169 273 699 2614 2862 3884 4863 169
Size: 104 426 1915 248 1022 979 486
Fragments arranged by size:
1915 1022 979 486 426 248 104
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1588 1620 1864 1896 3022 3052 3054 3084 1588
Size: 32 244 32 1126 30 2 30 3684
Fragments arranged by size:
3684 1126 244 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2219 2219
Size: 5180
KasI G'GCGC_C
Cuts at: 269 2610 2858 269
Size: 2341 248 2591
Fragments arranged by size:
2591 2341 248
KpnI G_GTAC'C
Cuts at: 2200 2200
Size: 5180
MluI A'CGCG_T
Cuts at: 4613 4613
Size: 5180
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 440 1023 3851 4035 440
Size: 583 2828 184 1585
Fragments arranged by size:
2828 1585 583 184
MscI TGG'CCA
Cuts at: 1036 2941 1036
Size: 1905 3275
MslI CAynn'nnrTG
Cuts at: 307 1867 1894 3169 3296 307
Size: 1560 27 1275 127 2191
Fragments arranged by size:
2191 1560 1275 127 27
MspA1I CmG'CkG
Cuts at: 563 708 1171 2291 2605 2965 3978 4223 563
Size: 145 463 1120 314 360 1013 245 1520
Fragments arranged by size:
1520 1120 1013 463 360 314 245 145
NaeI GCC'GGC
Cuts at: 448 3361 448
Size: 2913 2267
NarI GG'CG_CC
Cuts at: 270 2611 2859 270
Size: 2341 248 2591
Fragments arranged by size:
2591 2341 248
NgoMIV G'CCGG_C
Cuts at: 446 3359 446
Size: 2913 2267
NruI TCG'CGA
Cuts at: 297 297
Size: 5180
NspI r_CATG'y
Cuts at: 510 918 1681 2233 2733 3640 510
Size: 408 763 552 500 907 2050
Fragments arranged by size:
2050 907 763 552 500 408
PasI CC'CwG_GG
Cuts at: 465 663 465
Size: 198 4982
PciI A'CATG_T
Cuts at: 506 3636 506
Size: 3130 2050
PflMI CCAn_nnn'nTGG
Cuts at: 1068 1068
Size: 5180
PfoI T'CCnGG_A
Cuts at: 254 1964 2027 254
Size: 1710 63 3407
Fragments arranged by size:
3407 1710 63
PmeI GTTT'AAAC
Cuts at: 2179 2179
Size: 5180
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4319 4351 4319
Size: 32 5148
PpuMI rG'GwC_Cy
Cuts at: 627 1073 1230 2049 627
Size: 446 157 819 3758
Fragments arranged by size:
3758 819 446 157
PspOMI G'GGCC_C
Cuts at: 666 666
Size: 5180
PstI C_TGCA'G
Cuts at: 707 725 1580 1661 2105 2227 2912 707
Size: 18 855 81 444 122 685 2975
Fragments arranged by size:
2975 855 685 444 122 81 18
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5180
PvuII CAG'CTG
Cuts at: 563 708 2605 2965 563
Size: 145 1897 360 2778
Fragments arranged by size:
2778 1897 360 145
RsrII CG'GwC_CG
Cuts at: 3375 3375
Size: 5180
SacI G_AGCT'C
Cuts at: 730 2081 2194 730
Size: 1351 113 3716
Fragments arranged by size:
3716 1351 113
SalI G'TCGA_C
Cuts at: 2217 2217
Size: 5180
SanDI GG'GwC_CC
Cuts at: 2049 2049
Size: 5180
SapI GCTCTTCn'nnn_
Cuts at: 1477 3203 3413 1477
Size: 1726 210 3244
Fragments arranged by size:
3244 1726 210
SbfI CC_TGCA'GG
Cuts at: 1580 2227 1580
Size: 647 4533
ScaI AGT'ACT
Cuts at: 4697 4697
Size: 5180
SexAI A'CCwGG_T
Cuts at: 1538 1538
Size: 5180
SfcI C'TryA_G
Cuts at: 33 703 721 1576 1657 2101 2223 2908 3901
Size: 670 18 855 81 444 122 685 993
Cuts at: 3901 4092 33
Size: 191 1121
Fragments arranged by size:
1121 993 855 685 670 444 191 122
81 18
SfoI GGC'GCC
Cuts at: 271 2612 2860 271
Size: 2341 248 2591
Fragments arranged by size:
2591 2341 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5180
SmaI CCC'GGG
Cuts at: 880 1399 2202 880
Size: 519 803 3858
Fragments arranged by size:
3858 803 519
SmlI C'TyrA_G
Cuts at: 2335 3250 3742 4004 2335
Size: 915 492 262 3511
Fragments arranged by size:
3511 915 492 262
SnaBI TAC'GTA
Cuts at: 2172 2172
Size: 5180
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5180
SphI G_CATG'C
Cuts at: 1681 2233 2733 1681
Size: 552 500 4128
Fragments arranged by size:
4128 552 500
StyI C'CwwG_G
Cuts at: 1037 1244 2314 2668 1037
Size: 207 1070 354 3549
Fragments arranged by size:
3549 1070 354 207
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 700 1548 1995 2763 2896 3355 3564 700
Size: 848 447 768 133 459 209 2316
Fragments arranged by size:
2316 848 768 459 447 209 133
TatI w'GTAC_w
Cuts at: 1526 4695 1526
Size: 3169 2011
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 161 193 927 959 1990 2022 4697 4729 161
Size: 32 734 32 1031 32 2675 32 612
Fragments arranged by size:
2675 1031 734 612 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1600 2977 1600
Size: 1377 3803
XbaI T'CTAG_A
Cuts at: 2211 2211
Size: 5180
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 282 330 1251 2675 282
Size: 48 921 1424 2787
Fragments arranged by size:
2787 1424 921 48
XmaI C'CCGG_G
Cuts at: 878 1397 2200 878
Size: 519 803 3858
Fragments arranged by size:
3858 803 519
ZraI GAC'GTC
Cuts at: 1788 1788
Size: 5180
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NruI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PspOMI
PstI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SnaBI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AflII AhdI AloI AscI AvrII BaeI BcgI BfrBI
BmgBI BmtI BsiWI BsmI BssHII BstZ17I ClaI FseI
FspAI HindIII HpaI MfeI NcoI NdeI NheI NotI
NsiI PacI PmlI PshAI PsiI PspXI PsrI SacII
SfiI SgrAI SrfI SspI StuI SwaI XhoI XmnI