Restriction map for
pF1KSDB0064
(Circular) (Six-base) MAPSORT of: pF1KSDB0064.seq Check: 3487 from: 1 to: 5635
pF1KSDB0064 5635 bp
With 182 enzymes: SgfI *
June 20, 2008 10:36 ..
AccI GT'mk_AC
Cuts at: 2671 2671
Size: 5635
Acc65I G'GTAC_C
Cuts at: 2649 2649
Size: 5635
AclI AA'CG_TT
Cuts at: 2147 5475 2147
Size: 3328 2307
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1082 2492 3458 3890 4639 4907 1082
Size: 1410 966 432 749 268 1810
Fragments arranged by size:
1810 1410 966 749 432 268
AflIII A'CryG_T
Cuts at: 1101 2004 2985 4091 5068 1101
Size: 903 981 1106 977 1668
Fragments arranged by size:
1668 1106 981 977 903
AgeI A'CCGG_T
Cuts at: 4023 4023
Size: 5635
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2260 2260
Size: 5635
AlwNI CAG_nnn'CTG
Cuts at: 2313 2938 4507 2313
Size: 625 1569 3441
Fragments arranged by size:
3441 1569 625
ApaI G_GGCC'C
Cuts at: 1432 1432
Size: 5635
ApaLI G'TGCA_C
Cuts at: 4405 4405
Size: 5635
ApoI r'AATT_y
Cuts at: 221 484 1986 2068 2088 2637 4988 5244 221
Size: 263 1502 82 20 549 2351 256 612
Fragments arranged by size:
2351 1502 612 549 263 256 82 20
AseI AT'TA_AT
Cuts at: 20 827 20
Size: 807 4828
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5635
AvaI C'yCGr_G
Cuts at: 406 618 1425 2653 406
Size: 212 807 1228 3388
Fragments arranged by size:
3388 1228 807 212
BamHI G'GATC_C
Cuts at: 928 2658 928
Size: 1730 3905
BanI G'GyrC_C
Cuts at: 324 2649 3063 3311 3346 324
Size: 2325 414 248 35 2613
Fragments arranged by size:
2613 2325 414 248 35
BanII G_rGCy'C
Cuts at: 288 1432 1806 2647 3677 288
Size: 1144 374 841 1030 2246
Fragments arranged by size:
2246 1144 1030 841 374
BbeI G_GCGC'C
Cuts at: 3067 3315 3067
Size: 248 5387
BbsI GAAGACnn'nnnn_
Cuts at: 253 781 253
Size: 528 5107
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1866 1900 1866
Size: 34 5601
BciVI GTATCCnnnnn_n'
Cuts at: 3531 4294 5635 3531
Size: 763 1341 3531
Fragments arranged by size:
3531 1341 763
BclI T'GATC_A
Cuts at: 3153 3153
Size: 5635
BglI GCCn_nnn'nGGC
Cuts at: 1193 1230 1193
Size: 37 5598
BglII A'GATC_T
Cuts at: 3148 5187 3148
Size: 2039 3596
BlpI GC'TnA_GC
Cuts at: 1289 2745 1289
Size: 1456 4179
Bme1580I G_kGCm'C
Cuts at: 327 1432 3258 3351 4409 327
Size: 1105 1826 93 1058 1553
Fragments arranged by size:
1826 1553 1105 1058 93
BmgBI CAC'GTC
Cuts at: 2007 2007
Size: 5635
BmrI ACTGGGnnnn_n'
Cuts at: 2845 2926 3261 5407 2845
Size: 81 335 2146 3073
Fragments arranged by size:
3073 2146 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4000 4000
Size: 5635
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 668 692 1166 1538 1586 2329 668
Size: 24 474 372 48 743 3974
Fragments arranged by size:
3974 743 474 372 48 24
Bpu10I CC'TnA_GC
Cuts at: 374 1635 1805 374
Size: 1261 170 4204
Fragments arranged by size:
4204 1261 170
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1701 2809 3688 4182 4480 1701
Size: 1108 879 494 298 2856
Fragments arranged by size:
2856 1108 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 5339 5339
Size: 5635
BsaAI yAC'GTr
Cuts at: 2625 2986 2625
Size: 361 5274
BsaBI GATnn'nnATC
Cuts at: 6 3147 5186 6
Size: 3141 2039 455
Fragments arranged by size:
3141 2039 455
BsaHI Gr'CG_yC
Cuts at: 3064 3312 4016 5512 3064
Size: 248 704 1496 3187
Fragments arranged by size:
3187 1496 704 248
BsaWI w'CCGG_w
Cuts at: 1110 1897 2822 3343 4023 4297 4444 4855 1110
Size: 787 925 521 680 274 147 411 1890
Fragments arranged by size:
1890 925 787 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 666 696 1356 1386 1497 1527 1892 1922 666
Size: 30 660 30 111 30 365 30 4379
Fragments arranged by size:
4379 660 365 111 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 250 722 1226 1511 1610 2345 250
Size: 472 504 285 99 735 3540
Fragments arranged by size:
3540 735 504 472 285 99
BseYI C'CCAG_C
Cuts at: 1164 1176 1783 1959 3058 3816 4395 4891 1164
Size: 12 607 176 1099 758 579 496 1908
Fragments arranged by size:
1908 1099 758 607 579 496 176 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2326 2524 2326
Size: 198 5437
BsiEI CG_ry'CG
Cuts at: 104 3175 3221 4431 104
Size: 3071 46 1210 1308
Fragments arranged by size:
3071 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 1806 2357 2647 3425 3615 4409 1806
Size: 551 290 778 190 794 3032
Fragments arranged by size:
3032 794 778 551 290 190
BsmI GAATG_Cn'
Cuts at: 537 2183 2379 537
Size: 1646 196 3793
Fragments arranged by size:
3793 1646 196
Bsp1286I G_dGCh'C
Cuts at: 288 327 1432 1806 2357 2647 3258 3351 3425
Size: 39 1105 374 551 290 611 93 74
Cuts at: 3425 3615 3677 4409 288
Size: 190 62 732 1514
Fragments arranged by size:
1514 1105 732 611 551 374 290 190
93 74 62 39
BspEI T'CCGG_A
Cuts at: 1110 4855 1110
Size: 3745 1890
BspHI T'CATG_A
Cuts at: 4811 4811
Size: 5635
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1985 2252 2683 2897 3199 3580 1985
Size: 267 431 214 302 381 4040
Fragments arranged by size:
4040 431 381 302 267 214
BsrBI CCG'CTC
Cuts at: 50 1257 3925 3979 5465 5630 50
Size: 1207 2668 54 1486 165 55
Fragments arranged by size:
2668 1486 1207 165 55 54
BsrDI GCAATG_nn'
Cuts at: 324 505 1248 2282 3545 324
Size: 181 743 1034 1263 2414
Fragments arranged by size:
2414 1263 1034 743 181
BsrFI r'CCGG_y
Cuts at: 3631 3812 4023 3631
Size: 181 211 5243
Fragments arranged by size:
5243 211 181
BssSI C'ACGA_G
Cuts at: 3904 4264 3904
Size: 360 5275
BstAPI GCAn_nnn'nTGC
Cuts at: 3010 3010
Size: 5635
BstBI TT'CG_AA
Cuts at: 110 110
Size: 5635
BstXI CCAn_nnnn'nTGG
Cuts at: 1485 2369 1485
Size: 884 4751
BstYI r'GATC_y
Cuts at: 1 928 2658 3125 3148 3483 3729 4743 4829
Size: 927 1730 467 23 335 246 1014 86
Cuts at: 4829 4841 5187 1
Size: 12 346 449
Fragments arranged by size:
1730 1014 927 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 1796 1796
Size: 5635
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 558 2235 3583 3764 3927 558
Size: 1677 1348 181 163 2266
Fragments arranged by size:
2266 1677 1348 181 163
BtsI GCAGTG_nn'
Cuts at: 886 2216 2357 2876 886
Size: 1330 141 519 3645
Fragments arranged by size:
3645 1330 519 141
ClaI AT'CG_AT
Cuts at: 2568 2568
Size: 5635
DraI TTT'AAA
Cuts at: 2632 2632
Size: 5635
DrdI GACnn_nn'nnGTC
Cuts at: 2332 3339 4199 2332
Size: 1007 860 3768
Fragments arranged by size:
3768 1007 860
EaeI y'GGCC_r
Cuts at: 1540 3218 3392 3783 3810 5157 1540
Size: 1678 174 391 27 1347 2018
Fragments arranged by size:
2018 1678 1347 391 174 27
EagI C'GGCC_G
Cuts at: 3218 3218
Size: 5635
EarI CTCTTCn'nnn_
Cuts at: 3656 3866 3656
Size: 210 5425
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3841 4153 4299 5445 3841
Size: 312 146 1146 4031
Fragments arranged by size:
4031 1146 312 146
EcoICRI GAG'CTC
Cuts at: 1804 2645 1804
Size: 841 4794
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 668 692 1082 1166 1538 1586 2329 2492 3458
Size: 24 390 84 372 48 743 163 966
Cuts at: 3458 3890 4639 4907 668
Size: 432 749 268 1396
Fragments arranged by size:
1396 966 749 743 432 390 372 268
163 84 48 24
EcoO109I rG'GnC_Cy
Cuts at: 1186 1204 1213 1222 1429 1780 2772 1186
Size: 18 9 9 207 351 992 4049
Fragments arranged by size:
4049 992 351 207 18 9 9
EcoRI G'AATT_C
Cuts at: 221 2068 2637 221
Size: 1847 569 3219
Fragments arranged by size:
3219 1847 569
EcoRV GAT'ATC
Cuts at: 2412 2412
Size: 5635
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 541 1673 2504 3652 541
Size: 1132 831 1148 2524
Fragments arranged by size:
2524 1148 1132 831
FspI TGC'GCA
Cuts at: 2248 3414 2248
Size: 1166 4469
HaeII r_GCGC'y
Cuts at: 3067 3315 4339 5318 3067
Size: 248 1024 979 3384
Fragments arranged by size:
3384 1024 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 588 620 2561 2593 3475 3505 3507 3537 588
Size: 32 1941 32 882 30 2 30 2686
Fragments arranged by size:
2686 1941 882 32 32 30 30 2
HincII GTy'rAC
Cuts at: 296 854 1118 1670 2151 2672 296
Size: 558 264 552 481 521 3259
Fragments arranged by size:
3259 558 552 521 481 264
HpaI GTT'AAC
Cuts at: 296 854 296
Size: 558 5077
KasI G'GCGC_C
Cuts at: 3063 3311 3063
Size: 248 5387
KpnI G_GTAC'C
Cuts at: 2653 2653
Size: 5635
MfeI C'AATT_G
Cuts at: 941 941
Size: 5635
MluI A'CGCG_T
Cuts at: 5068 5068
Size: 5635
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1551 2535 4306 4490 1551
Size: 984 1771 184 2696
Fragments arranged by size:
2696 1771 984 184
MscI TGG'CCA
Cuts at: 3394 3394
Size: 5635
MslI CAynn'nnrTG
Cuts at: 3622 3749 3622
Size: 127 5508
MspA1I CmG'CkG
Cuts at: 1168 1963 2744 3058 3418 4433 4678 1168
Size: 795 781 314 360 1015 245 2125
Fragments arranged by size:
2125 1015 795 781 360 314 245
NaeI GCC'GGC
Cuts at: 3814 3814
Size: 5635
NarI GG'CG_CC
Cuts at: 3064 3312 3064
Size: 248 5387
NcoI C'CATG_G
Cuts at: 1796 1796
Size: 5635
NgoMIV G'CCGG_C
Cuts at: 3812 3812
Size: 5635
NruI TCG'CGA
Cuts at: 3996 3996
Size: 5635
NspI r_CATG'y
Cuts at: 1105 2085 2157 2686 3186 4095 1105
Size: 980 72 529 500 909 2645
Fragments arranged by size:
2645 980 909 529 500 72
PasI CC'CwG_GG
Cuts at: 1219 2178 1219
Size: 959 4676
PciI A'CATG_T
Cuts at: 1101 4091 1101
Size: 2990 2645
PfoI T'CCnGG_A
Cuts at: 1554 1554
Size: 5635
PmeI GTTT'AAAC
Cuts at: 2632 2632
Size: 5635
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4774 4806 4774
Size: 32 5603
PpuMI rG'GwC_Cy
Cuts at: 1204 1780 1204
Size: 576 5059
PsiI TTA'TAA
Cuts at: 694 2144 694
Size: 1450 4185
PspOMI G'GGCC_C
Cuts at: 1428 1428
Size: 5635
PstI C_TGCA'G
Cuts at: 2601 2680 3365 2601
Size: 79 685 4871
Fragments arranged by size:
4871 685 79
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5635
PvuII CAG'CTG
Cuts at: 1168 1963 3058 3418 1168
Size: 795 1095 360 3385
Fragments arranged by size:
3385 1095 795 360
RsrII CG'GwC_CG
Cuts at: 3828 3828
Size: 5635
SacI G_AGCT'C
Cuts at: 1806 2647 1806
Size: 841 4794
SalI G'TCGA_C
Cuts at: 2670 2670
Size: 5635
SanDI GG'GwC_CC
Cuts at: 1780 1780
Size: 5635
SapI GCTCTTCn'nnn_
Cuts at: 3656 3866 3656
Size: 210 5425
SbfI CC_TGCA'GG
Cuts at: 2680 2680
Size: 5635
ScaI AGT'ACT
Cuts at: 5152 5152
Size: 5635
SfcI C'TryA_G
Cuts at: 33 197 393 542 1392 2597 2676 3361 4356
Size: 164 196 149 850 1205 79 685 995
Cuts at: 4356 4547 33
Size: 191 1121
Fragments arranged by size:
1205 1121 995 850 685 196 191 164
149 79
SfiI GGCCn_nnn'nGGCC
Cuts at: 1193 1230 1193
Size: 37 5598
SfoI GGC'GCC
Cuts at: 3065 3313 3065
Size: 248 5387
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5635
SmaI CCC'GGG
Cuts at: 620 2655 620
Size: 2035 3600
SmlI C'TyrA_G
Cuts at: 1680 2788 3703 4197 4459 1680
Size: 1108 915 494 262 2856
Fragments arranged by size:
2856 1108 915 494 262
SnaBI TAC'GTA
Cuts at: 2625 2625
Size: 5635
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5635
SphI G_CATG'C
Cuts at: 2686 3186 2686
Size: 500 5135
SspI AAT'ATT
Cuts at: 2393 2393
Size: 5635
StuI AGG'CCT
Cuts at: 160 861 160
Size: 701 4934
StyI C'CwwG_G
Cuts at: 1123 1796 2063 2767 3121 1123
Size: 673 267 704 354 3637
Fragments arranged by size:
3637 704 673 354 267
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1401 3216 3349 3808 4019 1401
Size: 1815 133 459 211 3017
Fragments arranged by size:
3017 1815 459 211 133
TatI w'GTAC_w
Cuts at: 1245 5150 1245
Size: 3905 1730
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 666 698 1434 1466 1497 1529 1892 1924 5152
Size: 32 736 32 31 32 363 32 3228
Cuts at: 5152 5184 666
Size: 32 1117
Fragments arranged by size:
3228 1117 736 363 32 32 32 32
32 31
Tth111I GACn'n_nGTC
Cuts at: 3430 3430
Size: 5635
XbaI T'CTAG_A
Cuts at: 2664 2664
Size: 5635
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 651 1005 2366 3128 651
Size: 354 1361 762 3158
Fragments arranged by size:
3158 1361 762 354
XmaI C'CCGG_G
Cuts at: 618 2653 618
Size: 2035 3600
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI ClaI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NruI NspI PasI PciI PfoI PmeI PpiI
PpuMI PsiI PspOMI PstI PvuI PvuII RsrII SacI
SalI SanDI SapI SbfI ScaI SfcI SfiI SfoI
SgfI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI AscI
AvrII BaeI BbvCI BfrBI BmtI BsiWI BsmBI BsrGI
BssHII BstEII BstZ17I Bsu36I CspCI DraIII EcoNI FseI
FspAI HindIII NdeI NheI NotI NsiI PacI PflMI
PmlI PshAI PspXI PsrI SacII SexAI SgrAI SrfI
SwaI XhoI XmnI ZraI