Restriction map for
pF1KSDB0060
(Circular) (Six-base) MAPSORT of: pF1KSDB0060.seq Check: 7706 from: 1 to: 5860
pF1KSDB0060 5860 bp
With 182 enzymes: SgfI *
June 20, 2008 10:36 ..
AccI GT'mk_AC
Cuts at: 1516 2896 1516
Size: 1380 4480
Acc65I G'GTAC_C
Cuts at: 1817 2818 2874 1817
Size: 1001 56 4803
Fragments arranged by size:
4803 1001 56
AclI AA'CG_TT
Cuts at: 155 5700 155
Size: 5545 315
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1279 2022 2464 2742 3683 4115 4864 5132 1279
Size: 743 442 278 941 432 749 268 2007
Fragments arranged by size:
2007 941 749 743 442 432 278 268
AflIII A'CryG_T
Cuts at: 2177 2843 3210 4316 5293 2177
Size: 666 367 1106 977 2744
Fragments arranged by size:
2744 1106 977 666 367
AgeI A'CCGG_T
Cuts at: 4248 4248
Size: 5860
AhdI GACnn_n'nnGTC
Cuts at: 144 144
Size: 5860
AlwNI CAG_nnn'CTG
Cuts at: 171 337 3163 4732 171
Size: 166 2826 1569 1299
Fragments arranged by size:
2826 1569 1299 166
ApaLI G'TGCA_C
Cuts at: 4630 4630
Size: 5860
ApoI r'AATT_y
Cuts at: 277 1188 1459 1596 1922 2629 2862 5213 5469
Size: 911 271 137 326 707 233 2351 256
Cuts at: 5469 277
Size: 668
Fragments arranged by size:
2351 911 707 668 326 271 256 233
137
AseI AT'TA_AT
Cuts at: 20 20
Size: 5860
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5860
AvaI C'yCGr_G
Cuts at: 2084 2878 2084
Size: 794 5066
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2676 2709 2676
Size: 33 5827
BamHI G'GATC_C
Cuts at: 182 2883 182
Size: 2701 3159
BanI G'GyrC_C
Cuts at: 1220 1817 2818 2874 3288 3536 3571 1220
Size: 597 1001 56 414 248 35 3509
Fragments arranged by size:
3509 1001 597 414 248 56 35
BanII G_rGCy'C
Cuts at: 586 2798 2872 3902 586
Size: 2212 74 1030 2544
Fragments arranged by size:
2544 2212 1030 74
BbeI G_GCGC'C
Cuts at: 1224 3292 3540 1224
Size: 2068 248 3544
Fragments arranged by size:
3544 2068 248
BbsI GAAGACnn'nnnn_
Cuts at: 1267 2028 2734 1267
Size: 761 706 4393
Fragments arranged by size:
4393 761 706
BbvCI CC'TCA_GC
Cuts at: 1317 1381 1317
Size: 64 5796
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 141 175 234 268 141
Size: 34 59 34 5733
Fragments arranged by size:
5733 59 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 831 3756 4519 5860 831
Size: 2925 763 1341 831
Fragments arranged by size:
2925 1341 831 763
BclI T'GATC_A
Cuts at: 1620 2463 3378 1620
Size: 843 915 4102
Fragments arranged by size:
4102 915 843
BfrBI ATG'CAT
Cuts at: 1711 1711
Size: 5860
BglII A'GATC_T
Cuts at: 3373 5412 3373
Size: 2039 3821
BlpI GC'TnA_GC
Cuts at: 418 2970 418
Size: 2552 3308
Bme1580I G_kGCm'C
Cuts at: 570 633 2436 3483 3576 4634 570
Size: 63 1803 1047 93 1058 1796
Fragments arranged by size:
1803 1796 1058 1047 93 63
BmgBI CAC'GTC
Cuts at: 1992 1992
Size: 5860
BmrI ACTGGGnnnn_n'
Cuts at: 564 788 3070 3151 3486 5632 564
Size: 224 2282 81 335 2146 792
Fragments arranged by size:
2282 2146 792 335 224 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1332 4225 1332
Size: 2893 2967
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 674 1997 2023 2148 2412 674
Size: 1323 26 125 264 4122
Fragments arranged by size:
4122 1323 264 125 26
Bpu10I CC'TnA_GC
Cuts at: 482 547 1317 1381 482
Size: 65 770 64 4961
Fragments arranged by size:
4961 770 65 64
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1219 1462 2474 3034 3913 4407 4705 1219
Size: 243 1012 560 879 494 298 2374
Fragments arranged by size:
2374 1012 879 560 494 298 243
BsaI GGTCTCn'nnnn_
Cuts at: 1340 1367 1738 2013 5564 1340
Size: 27 371 275 3551 1636
Fragments arranged by size:
3551 1636 371 275 27
BsaAI yAC'GTr
Cuts at: 2846 2850 3211 2846
Size: 4 361 5495
Fragments arranged by size:
5495 361 4
BsaBI GATnn'nnATC
Cuts at: 6 2357 3372 5411 6
Size: 2351 1015 2039 455
Fragments arranged by size:
2351 2039 1015 455
BsaHI Gr'CG_yC
Cuts at: 1221 3289 3537 4241 5737 1221
Size: 2068 248 704 1496 1344
Fragments arranged by size:
2068 1496 1344 704 248
BsaWI w'CCGG_w
Cuts at: 3047 3568 4248 4522 4669 5080 3047
Size: 521 680 274 147 411 3827
Fragments arranged by size:
3827 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 647 677 2410 2440 647
Size: 30 1733 30 4067
Fragments arranged by size:
4067 1733 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 655 1071 2620 655
Size: 416 1549 3895
Fragments arranged by size:
3895 1549 416
BseYI C'CCAG_C
Cuts at: 212 3283 4041 4620 5116 212
Size: 3071 758 579 496 956
Fragments arranged by size:
3071 956 758 579 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1390 2212 2357 2719 1390
Size: 822 145 362 4531
Fragments arranged by size:
4531 822 362 145
BsiEI CG_ry'CG
Cuts at: 104 3400 3446 4656 104
Size: 3296 46 1210 1308
Fragments arranged by size:
3296 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 586 2659 2872 3650 3840 4634 586
Size: 2073 213 778 190 794 1812
Fragments arranged by size:
2073 1812 794 778 213 190
BsiWI C'GTAC_G
Cuts at: 2846 2846
Size: 5860
BsmI GAATG_Cn'
Cuts at: 2490 2490
Size: 5860
BsmBI CGTCTCn'nnnn_
Cuts at: 2523 2523
Size: 5860
Bsp1286I G_dGCh'C
Cuts at: 570 586 633 2436 2659 2798 2872 3483 3576
Size: 16 47 1803 223 139 74 611 93
Cuts at: 3576 3650 3840 3902 4634 570
Size: 74 190 62 732 1796
Fragments arranged by size:
1803 1796 732 611 223 190 139 93
74 74 62 47 16
BspEI T'CCGG_A
Cuts at: 5080 5080
Size: 5860
BspHI T'CATG_A
Cuts at: 1617 5036 1617
Size: 3419 2441
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1829 2908 3122 3424 3805 1829
Size: 1079 214 302 381 3884
Fragments arranged by size:
3884 1079 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1070 4150 4204 5690 5855 50
Size: 1020 3080 54 1486 165 55
Fragments arranged by size:
3080 1486 1020 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1814 2190 2366 3770 1814
Size: 376 176 1404 3904
Fragments arranged by size:
3904 1404 376 176
BsrFI r'CCGG_y
Cuts at: 3856 4037 4248 3856
Size: 181 211 5468
Fragments arranged by size:
5468 211 181
BssSI C'ACGA_G
Cuts at: 1387 2377 4129 4489 1387
Size: 990 1752 360 2758
Fragments arranged by size:
2758 1752 990 360
BstAPI GCAn_nnn'nTGC
Cuts at: 337 3235 337
Size: 2898 2962
BstBI TT'CG_AA
Cuts at: 110 110
Size: 5860
BstYI r'GATC_y
Cuts at: 1 182 1476 1973 2883 3350 3373 3708 3954
Size: 181 1294 497 910 467 23 335 246
Cuts at: 3954 4968 5054 5066 5412 1
Size: 1014 86 12 346 449
Fragments arranged by size:
1294 1014 910 497 467 449 346 335
246 181 86 23 12
BstZ17I GTA'TAC
Cuts at: 1517 1517
Size: 5860
BtgI C'CryG_G
Cuts at: 125 125
Size: 5860
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3808 3989 4152 3808
Size: 181 163 5516
Fragments arranged by size:
5516 181 163
BtsI GCAGTG_nn'
Cuts at: 609 1284 3101 609
Size: 675 1817 3368
Fragments arranged by size:
3368 1817 675
ClaI AT'CG_AT
Cuts at: 1134 1531 1134
Size: 397 5463
DraI TTT'AAA
Cuts at: 2441 2634 2857 2441
Size: 193 223 5444
Fragments arranged by size:
5444 223 193
DraIII CAC_nnn'GTG
Cuts at: 2382 2382
Size: 5860
DrdI GACnn_nn'nnGTC
Cuts at: 997 3564 4424 997
Size: 2567 860 2433
Fragments arranged by size:
2567 2433 860
EaeI y'GGCC_r
Cuts at: 3443 3617 4008 4035 5382 3443
Size: 174 391 27 1347 3921
Fragments arranged by size:
3921 1347 391 174 27
EagI C'GGCC_G
Cuts at: 3443 3443
Size: 5860
EarI CTCTTCn'nnn_
Cuts at: 318 2540 2597 3881 4091 318
Size: 2222 57 1284 210 2087
Fragments arranged by size:
2222 2087 1284 210 57
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4066 4378 4524 5670 4066
Size: 312 146 1146 4256
Fragments arranged by size:
4256 1146 312 146
EcoICRI GAG'CTC
Cuts at: 584 2870 584
Size: 2286 3574
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 674 1279 1997 2022 2023 2148 2412 2464 2742
Size: 605 718 25 1 125 264 52 278
Cuts at: 2742 3683 4115 4864 5132 674
Size: 941 432 749 268 1402
Fragments arranged by size:
1402 941 749 718 605 432 278 268
264 125 52 25 1
EcoNI CCTnn'n_nnAGG
Cuts at: 427 427
Size: 5860
EcoO109I rG'GnC_Cy
Cuts at: 432 1572 2997 432
Size: 1140 1425 3295
Fragments arranged by size:
3295 1425 1140
EcoRI G'AATT_C
Cuts at: 1922 2862 1922
Size: 940 4920
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 998 1803 3877 998
Size: 805 2074 2981
Fragments arranged by size:
2981 2074 805
FspI TGC'GCA
Cuts at: 3639 3639
Size: 5860
HaeII r_GCGC'y
Cuts at: 1224 3292 3540 4564 5543 1224
Size: 2068 248 1024 979 1541
Fragments arranged by size:
2068 1541 1024 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 130 162 490 522 1227 1259 1542 1574 1887
Size: 32 328 32 705 32 283 32 313
Cuts at: 1887 1919 2018 2050 2410 2442 3700 3730 3732
Size: 32 99 32 360 32 1258 30 2
Cuts at: 3732 3762 130
Size: 30 2228
Fragments arranged by size:
2228 1258 705 360 328 313 283 99
32 32 32 32 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 2058 2897 2058
Size: 839 5021
KasI G'GCGC_C
Cuts at: 1220 3288 3536 1220
Size: 2068 248 3544
Fragments arranged by size:
3544 2068 248
KpnI G_GTAC'C
Cuts at: 1821 2822 2878 1821
Size: 1001 56 4803
Fragments arranged by size:
4803 1001 56
MfeI C'AATT_G
Cuts at: 2066 2066
Size: 5860
MluI A'CGCG_T
Cuts at: 5293 5293
Size: 5860
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1504 1742 4531 4715 1504
Size: 238 2789 184 2649
Fragments arranged by size:
2789 2649 238 184
MscI TGG'CCA
Cuts at: 3619 3619
Size: 5860
MslI CAynn'nnrTG
Cuts at: 925 3847 3974 925
Size: 2922 127 2811
Fragments arranged by size:
2922 2811 127
MspA1I CmG'CkG
Cuts at: 1321 1421 1936 2408 2969 3283 3643 4658 4903
Size: 100 515 472 561 314 360 1015 245
Cuts at: 4903 1321
Size: 2278
Fragments arranged by size:
2278 1015 561 515 472 360 314 245
100
NaeI GCC'GGC
Cuts at: 4039 4039
Size: 5860
NarI GG'CG_CC
Cuts at: 1221 3289 3537 1221
Size: 2068 248 3544
Fragments arranged by size:
3544 2068 248
NcoI C'CATG_G
Cuts at: 125 125
Size: 5860
NgoMIV G'CCGG_C
Cuts at: 4037 4037
Size: 5860
NruI TCG'CGA
Cuts at: 4221 4221
Size: 5860
NsiI A_TGCA'T
Cuts at: 1713 1713
Size: 5860
NspI r_CATG'y
Cuts at: 1359 1803 2181 2208 2911 3411 4320 1359
Size: 444 378 27 703 500 909 2899
Fragments arranged by size:
2899 909 703 500 444 378 27
PciI A'CATG_T
Cuts at: 2177 4316 2177
Size: 2139 3721
PflMI CCAn_nnn'nTGG
Cuts at: 2355 2355
Size: 5860
PfoI T'CCnGG_A
Cuts at: 690 823 690
Size: 133 5727
PmeI GTTT'AAAC
Cuts at: 2857 2857
Size: 5860
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4999 5031 4999
Size: 32 5828
PpuMI rG'GwC_Cy
Cuts at: 432 1572 432
Size: 1140 4720
PshAI GACnn'nnGTC
Cuts at: 2738 2738
Size: 5860
PsiI TTA'TAA
Cuts at: 1846 1846
Size: 5860
PstI C_TGCA'G
Cuts at: 167 181 1665 1686 2905 3590 167
Size: 14 1484 21 1219 685 2437
Fragments arranged by size:
2437 1484 1219 685 21 14
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5860
PvuII CAG'CTG
Cuts at: 1321 3283 3643 1321
Size: 1962 360 3538
Fragments arranged by size:
3538 1962 360
RsrII CG'GwC_CG
Cuts at: 4053 4053
Size: 5860
SacI G_AGCT'C
Cuts at: 586 2872 586
Size: 2286 3574
SalI G'TCGA_C
Cuts at: 2895 2895
Size: 5860
SapI GCTCTTCn'nnn_
Cuts at: 3881 4091 3881
Size: 210 5650
SbfI CC_TGCA'GG
Cuts at: 2905 2905
Size: 5860
ScaI AGT'ACT
Cuts at: 5377 5377
Size: 5860
SfcI C'TryA_G
Cuts at: 33 163 177 1661 1682 2901 3586 4581 4772
Size: 130 14 1484 21 1219 685 995 191
Cuts at: 4772 33
Size: 1121
Fragments arranged by size:
1484 1219 1121 995 685 191 130 21
14
SfoI GGC'GCC
Cuts at: 1222 3290 3538 1222
Size: 2068 248 3544
Fragments arranged by size:
3544 2068 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5860
SmaI CCC'GGG
Cuts at: 2880 2880
Size: 5860
SmlI C'TyrA_G
Cuts at: 1198 1441 2084 2489 3013 3928 4422 4684 1198
Size: 243 643 405 524 915 494 262 2374
Fragments arranged by size:
2374 915 643 524 494 405 262 243
SnaBI TAC'GTA
Cuts at: 2850 2850
Size: 5860
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5860
SphI G_CATG'C
Cuts at: 2911 3411 2911
Size: 500 5360
SspI AAT'ATT
Cuts at: 2672 2672
Size: 5860
StuI AGG'CCT
Cuts at: 487 531 487
Size: 44 5816
StyI C'CwwG_G
Cuts at: 125 1290 2992 3346 125
Size: 1165 1702 354 2639
Fragments arranged by size:
2639 1702 1165 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3441 3574 4033 4244 3441
Size: 133 459 211 5057
Fragments arranged by size:
5057 459 211 133
TatI w'GTAC_w
Cuts at: 1781 5375 1781
Size: 3594 2266
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 647 679 1290 1322 5377 5409 647
Size: 32 611 32 4055 32 1098
Fragments arranged by size:
4055 1098 611 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 819 1656 3655 819
Size: 837 1999 3024
Fragments arranged by size:
3024 1999 837
XbaI T'CTAG_A
Cuts at: 1926 2889 1926
Size: 963 4897
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3353 3353
Size: 5860
XhoI C'TCGA_G
Cuts at: 2084 2084
Size: 5860
XmaI C'CCGG_G
Cuts at: 2878 2878
Size: 5860
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlwNI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI BstZ17I BtgI BtgZI BtsI ClaI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NruI NsiI NspI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AlfI AloI ApaI
AscI AvrII BglI BmtI BsrGI BssHII BstEII BstXI
Bsu36I CspCI EcoRV FseI FspAI HindIII HpaI NdeI
NheI NotI PacI PasI PmlI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SrfI SwaI XmnI
ZraI