Restriction map for
pF1KSDB0046
(Circular) (Six-base) MAPSORT of: pF1KSDB0046.seq Check: 2451 from: 1 to: 9064
pF1KSDB0046 9064 bp
With 182 enzymes: SgfI *
June 20, 2008 10:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 915 915
Size: 9064
AccI GT'mk_AC
Cuts at: 5458 6100 5458
Size: 642 8422
Acc65I G'GTAC_C
Cuts at: 6078 6078
Size: 9064
AclI AA'CG_TT
Cuts at: 8904 8904
Size: 9064
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 243 575 641 1202 1355 1601 2102 2375 3980
Size: 332 66 561 153 246 501 273 1605
Cuts at: 3980 4016 4431 4787 5180 5770 5947 5957 6887
Size: 36 415 356 393 590 177 10 930
Cuts at: 6887 7319 8068 8336 243
Size: 432 749 268 971
Fragments arranged by size:
1605 971 930 749 590 561 501 432
415 393 356 332 273 268 246 177
153 66 36 10
AflIII A'CryG_T
Cuts at: 439 879 1319 6414 7520 8497 439
Size: 440 440 5095 1106 977 1006
Fragments arranged by size:
5095 1106 1006 977 440 440
AgeI A'CCGG_T
Cuts at: 5260 7452 5260
Size: 2192 6872
AleI CACnn'nnGTG
Cuts at: 1324 4118 4768 5967 1324
Size: 2794 650 1199 4421
Fragments arranged by size:
4421 2794 1199 650
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1702 1702
Size: 9064
AlwNI CAG_nnn'CTG
Cuts at: 415 1030 2125 2599 2614 2790 3637 5485 5748
Size: 615 1095 474 15 176 847 1848 263
Cuts at: 5748 5830 6367 7936 415
Size: 82 537 1569 1543
Fragments arranged by size:
1848 1569 1543 1095 847 615 537 474
263 176 82 15
ApaLI G'TGCA_C
Cuts at: 7834 7834
Size: 9064
ApoI r'AATT_y
Cuts at: 1212 1635 1813 4532 5425 5435 5708 6066 8417
Size: 423 178 2719 893 10 273 358 2351
Cuts at: 8417 8673 1212
Size: 256 1603
Fragments arranged by size:
2719 2351 1603 893 423 358 273 256
178 10
AseI AT'TA_AT
Cuts at: 20 20
Size: 9064
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 9064
AvaI C'yCGr_G
Cuts at: 4202 4463 6082 4202
Size: 261 1619 7184
Fragments arranged by size:
7184 1619 261
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4698 4731 4698
Size: 33 9031
BamHI G'GATC_C
Cuts at: 3294 6087 3294
Size: 2793 6271
BanI G'GyrC_C
Cuts at: 2921 6078 6492 6740 6775 2921
Size: 3157 414 248 35 5210
Fragments arranged by size:
5210 3157 414 248 35
BanII G_rGCy'C
Cuts at: 2939 4024 4607 5239 5795 6076 7106 2939
Size: 1085 583 632 556 281 1030 4897
Fragments arranged by size:
4897 1085 1030 632 583 556 281
BbeI G_GCGC'C
Cuts at: 6496 6744 6496
Size: 248 8816
BbsI GAAGACnn'nnnn_
Cuts at: 1058 1458 2108 2239 2741 3238 3524 5533 1058
Size: 400 650 131 502 497 286 2009 4589
Fragments arranged by size:
4589 2009 650 502 497 400 286 131
BbvCI CC'TCA_GC
Cuts at: 476 3824 476
Size: 3348 5716
BciVI GTATCCnnnnn_n'
Cuts at: 1773 1849 6960 7723 9064 1773
Size: 76 5111 763 1341 1773
Fragments arranged by size:
5111 1773 1341 763 76
BclI T'GATC_A
Cuts at: 6582 6582
Size: 9064
BfrBI ATG'CAT
Cuts at: 1581 1581
Size: 9064
BglI GCCn_nnn'nGGC
Cuts at: 4329 4400 4329
Size: 71 8993
BglII A'GATC_T
Cuts at: 1221 2583 6577 8616 1221
Size: 1362 3994 2039 1669
Fragments arranged by size:
3994 2039 1669 1362
BlpI GC'TnA_GC
Cuts at: 2764 6174 2764
Size: 3410 5654
Bme1580I G_kGCm'C
Cuts at: 2924 4463 5856 6687 6780 7838 2924
Size: 1539 1393 831 93 1058 4150
Fragments arranged by size:
4150 1539 1393 1058 831 93
BmgBI CAC'GTC
Cuts at: 1322 3081 1322
Size: 1759 7305
BmrI ACTGGGnnnn_n'
Cuts at: 860 2487 4025 5511 6274 6355 6690 8836 860
Size: 1627 1538 1486 763 81 335 2146 1088
Fragments arranged by size:
2146 1627 1538 1486 1088 763 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2191 2499 7429 2191
Size: 308 4930 3826
Fragments arranged by size:
4930 3826 308
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 274 368 1781 2385 2498 4298 4511 4568 5049
Size: 94 1413 604 113 1800 213 57 481
Cuts at: 5049 5638 5809 274
Size: 589 171 3529
Fragments arranged by size:
3529 1800 1413 604 589 481 213 171
113 94 57
Bpu10I CC'TnA_GC
Cuts at: 476 3824 4128 476
Size: 3348 304 5412
Fragments arranged by size:
5412 3348 304
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1752 3329 3470 3642 5716 6238 7117 7611 7909
Size: 1577 141 172 2074 522 879 494 298
Cuts at: 7909 1752
Size: 2907
Fragments arranged by size:
2907 2074 1577 879 522 494 298 172
141
BsaI GGTCTCn'nnnn_
Cuts at: 3019 8768 3019
Size: 5749 3315
BsaAI yAC'GTr
Cuts at: 6054 6415 6054
Size: 361 8703
BsaBI GATnn'nnATC
Cuts at: 6 6576 8615 6
Size: 6570 2039 455
Fragments arranged by size:
6570 2039 455
BsaHI Gr'CG_yC
Cuts at: 4255 6493 6741 7445 8941 4255
Size: 2238 248 704 1496 4378
Fragments arranged by size:
4378 2238 1496 704 248
BsaWI w'CCGG_w
Cuts at: 5260 6251 6772 7452 7726 7873 8284 5260
Size: 991 521 680 274 147 411 6040
Fragments arranged by size:
6040 991 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 4777 4807 5026 5056 5216 5246 5782 5812 4777
Size: 30 219 30 160 30 536 30 8029
Fragments arranged by size:
8029 536 219 160 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 374 1358 2122 3370 3967 4014 4047 4220 4282
Size: 984 764 1248 597 47 33 173 62
Cuts at: 4282 4569 5434 5713 374
Size: 287 865 279 3725
Fragments arranged by size:
3725 1248 984 865 764 597 287 279
173 62 47 33
BseYI C'CCAG_C
Cuts at: 1496 2790 3580 4195 4675 5046 5468 5683 5913
Size: 1294 790 615 480 371 422 215 230
Cuts at: 5913 6487 7245 7824 8320 1496
Size: 574 758 579 496 2240
Fragments arranged by size:
2240 1294 790 758 615 579 574 496
480 422 371 230 215
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 965 4823 5843 965
Size: 3858 1020 4186
Fragments arranged by size:
4186 3858 1020
BsiEI CG_ry'CG
Cuts at: 104 6604 6650 7860 104
Size: 6500 46 1210 1308
Fragments arranged by size:
6500 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 1459 2485 3768 5795 6076 6854 7044 7838 1459
Size: 1026 1283 2027 281 778 190 794 2685
Fragments arranged by size:
2685 2027 1283 1026 794 778 281 190
BsmI GAATG_Cn'
Cuts at: 2278 2278
Size: 9064
BsmBI CGTCTCn'nnnn_
Cuts at: 356 356
Size: 9064
Bsp1286I G_dGCh'C
Cuts at: 1459 2485 2924 2939 3768 4024 4463 4607 5239
Size: 1026 439 15 829 256 439 144 632
Cuts at: 5239 5795 5856 6076 6687 6780 6854 7044 7106
Size: 556 61 220 611 93 74 190 62
Cuts at: 7106 7838 1459
Size: 732 2685
Fragments arranged by size:
2685 1026 829 732 632 611 556 439
439 256 220 190 144 93 74 62
61 15
BspEI T'CCGG_A
Cuts at: 8284 8284
Size: 9064
BspHI T'CATG_A
Cuts at: 1512 5810 8240 1512
Size: 4298 2430 2336
Fragments arranged by size:
4298 2430 2336
BspMI ACCTGCnnnn'nnnn_
Cuts at: 915 6112 6326 6628 7009 915
Size: 5197 214 302 381 2970
Fragments arranged by size:
5197 2970 381 302 214
BsrBI CCG'CTC
Cuts at: 50 5378 7354 7408 8894 9059 50
Size: 5328 1976 54 1486 165 55
Fragments arranged by size:
5328 1976 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3503 5941 6974 3503
Size: 2438 1033 5593
Fragments arranged by size:
5593 2438 1033
BsrFI r'CCGG_y
Cuts at: 5260 7060 7241 7452 5260
Size: 1800 181 211 6872
Fragments arranged by size:
6872 1800 211 181
BsrGI T'GTAC_A
Cuts at: 876 876
Size: 9064
BssSI C'ACGA_G
Cuts at: 3893 4325 4661 5111 7333 7693 3893
Size: 432 336 450 2222 360 5264
Fragments arranged by size:
5264 2222 450 432 360 336
BstAPI GCAn_nnn'nTGC
Cuts at: 949 1164 4186 6439 949
Size: 215 3022 2253 3574
Fragments arranged by size:
3574 3022 2253 215
BstBI TT'CG_AA
Cuts at: 110 110
Size: 9064
BstEII G'GTnAC_C
Cuts at: 3376 4030 3376
Size: 654 8410
BstXI CCAn_nnnn'nTGG
Cuts at: 3693 3706 4758 3693
Size: 13 1052 7999
Fragments arranged by size:
7999 1052 13
BstYI r'GATC_y
Cuts at: 1 1221 1647 2583 3193 3294 5804 6087 6554
Size: 1220 426 936 610 101 2510 283 467
Cuts at: 6554 6577 6912 7158 8172 8258 8270 8616 1
Size: 23 335 246 1014 86 12 346 449
Fragments arranged by size:
2510 1220 1014 936 610 467 449 426
346 335 283 246 101 86 23 12
BstZ17I GTA'TAC
Cuts at: 5459 5459
Size: 9064
Bsu36I CC'TnA_GG
Cuts at: 1031 1643 4026 1031
Size: 612 2383 6069
Fragments arranged by size:
6069 2383 612
BtgI C'CryG_G
Cuts at: 3608 5120 5345 3608
Size: 1512 225 7327
Fragments arranged by size:
7327 1512 225
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 7012 7193 7356 7012
Size: 181 163 8720
Fragments arranged by size:
8720 181 163
BtsI GCAGTG_nn'
Cuts at: 501 4125 5415 6305 501
Size: 3624 1290 890 3260
Fragments arranged by size:
3624 3260 1290 890
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 4858 4893 4858
Size: 35 9029
DraI TTT'AAA
Cuts at: 6061 6061
Size: 9064
DraIII CAC_nnn'GTG
Cuts at: 2155 2155
Size: 9064
DrdI GACnn_nn'nnGTC
Cuts at: 6768 7628 6768
Size: 860 8204
EaeI y'GGCC_r
Cuts at: 3837 5348 5369 6647 6821 7212 7239 8586 3837
Size: 1511 21 1278 174 391 27 1347 4315
Fragments arranged by size:
4315 1511 1347 1278 391 174 27 21
EagI C'GGCC_G
Cuts at: 6647 6647
Size: 9064
EarI CTCTTCn'nnn_
Cuts at: 533 545 3134 3347 3803 3956 5156 5276 5411
Size: 12 2589 213 456 153 1200 120 135
Cuts at: 5411 7085 7295 533
Size: 1674 210 2302
Fragments arranged by size:
2589 2302 1674 1200 456 213 210 153
135 120 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 7270 7582 7728 8874 7270
Size: 312 146 1146 7460
Fragments arranged by size:
7460 1146 312 146
EcoICRI GAG'CTC
Cuts at: 5793 6074 5793
Size: 281 8783
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 243 274 368 575 641 1202 1355 1601 1781
Size: 31 94 207 66 561 153 246 180
Cuts at: 1781 2102 2375 2385 2498 3980 4016 4298 4431
Size: 321 273 10 113 1482 36 282 133
Cuts at: 4431 4511 4568 4787 5049 5180 5638 5770 5809
Size: 80 57 219 262 131 458 132 39
Cuts at: 5809 5947 5957 6887 7319 8068 8336 243
Size: 138 10 930 432 749 268 971
Fragments arranged by size:
1482 971 930 749 561 458 432 321
282 273 268 262 246 219 207 180
153 138 133 132 131 113 94 80
66 57 39 36 31 10 10
EcoNI CCTnn'n_nnAGG
Cuts at: 1192 2870 4232 4783 1192
Size: 1678 1362 551 5473
Fragments arranged by size:
5473 1678 1362 551
EcoO109I rG'GnC_Cy
Cuts at: 1184 3432 3627 5293 6201 1184
Size: 2248 195 1666 908 4047
Fragments arranged by size:
4047 2248 1666 908 195
EcoRI G'AATT_C
Cuts at: 1212 1635 5708 6066 1212
Size: 423 4073 358 4210
Fragments arranged by size:
4210 4073 423 358
EcoRV GAT'ATC
Cuts at: 816 2880 4044 4989 816
Size: 2064 1164 945 4891
Fragments arranged by size:
4891 2064 1164 945
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 5775 7081 5775
Size: 1306 7758
FspI TGC'GCA
Cuts at: 6843 6843
Size: 9064
HaeII r_GCGC'y
Cuts at: 6496 6744 7768 8747 6496
Size: 248 1024 979 6813
Fragments arranged by size:
6813 1024 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 310 342 616 648 1024 1056 1081 1113 2098
Size: 32 274 32 376 32 25 32 985
Cuts at: 2098 2130 2211 2243 2479 2511 2575 2607 2992
Size: 32 81 32 236 32 64 32 385
Cuts at: 2992 3018 3024 3050 3769 3801 4033 4065 4426
Size: 26 6 26 719 32 232 32 361
Cuts at: 4426 4458 4564 4596 5155 5187 5782 5814 6904
Size: 32 106 32 559 32 595 32 1090
Cuts at: 6904 6934 6936 6966 310
Size: 30 2 30 2408
Fragments arranged by size:
2408 1090 985 719 595 559 385 376
361 274 236 232 106 81 64 32
32 32 32 32 32 32 32 32
32 32 32 32 32 30 30 26
26 25 6 2
HincII GTy'rAC
Cuts at: 1043 3400 6101 1043
Size: 2357 2701 4006
Fragments arranged by size:
4006 2701 2357
HindIII A'AGCT_T
Cuts at: 2372 2820 2372
Size: 448 8616
KasI G'GCGC_C
Cuts at: 6492 6740 6492
Size: 248 8816
KpnI G_GTAC'C
Cuts at: 6082 6082
Size: 9064
MfeI C'AATT_G
Cuts at: 3968 3968
Size: 9064
MluI A'CGCG_T
Cuts at: 8497 8497
Size: 9064
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 143 296 536 830 4650 7735 7919 143
Size: 153 240 294 3820 3085 184 1288
Fragments arranged by size:
3820 3085 1288 294 240 184 153
MscI TGG'CCA
Cuts at: 3839 5350 5371 6823 3839
Size: 1511 21 1452 6080
Fragments arranged by size:
6080 1511 1452 21
MslI CAynn'nnrTG
Cuts at: 850 1324 3241 3839 4118 4768 5849 5967 6038
Size: 474 1917 598 279 650 1081 118 71
Cuts at: 6038 7051 7178 850
Size: 1013 127 2736
Fragments arranged by size:
2736 1917 1081 1013 650 598 474 279
127 118 71
MspA1I CmG'CkG
Cuts at: 346 4166 4384 4758 5683 5949 6173 6487 6847
Size: 3820 218 374 925 266 224 314 360
Cuts at: 6847 7862 8107 346
Size: 1015 245 1303
Fragments arranged by size:
3820 1303 1015 925 374 360 314 266
245 224 218
NaeI GCC'GGC
Cuts at: 7243 7243
Size: 9064
NarI GG'CG_CC
Cuts at: 6493 6741 6493
Size: 248 8816
NcoI C'CATG_G
Cuts at: 3608 5120 5345 3608
Size: 1512 225 7327
Fragments arranged by size:
7327 1512 225
NdeI CA'TA_TG
Cuts at: 702 702
Size: 9064
NgoMIV G'CCGG_C
Cuts at: 7241 7241
Size: 9064
NruI TCG'CGA
Cuts at: 7425 7425
Size: 9064
NsiI A_TGCA'T
Cuts at: 1583 1583
Size: 9064
NspI r_CATG'y
Cuts at: 443 883 927 6115 6615 7524 443
Size: 440 44 5188 500 909 1983
Fragments arranged by size:
5188 1983 909 500 440 44
PciI A'CATG_T
Cuts at: 439 879 7520 439
Size: 440 6641 1983
Fragments arranged by size:
6641 1983 440
PflMI CCAn_nnn'nTGG
Cuts at: 2352 2790 2896 3459 3609 2352
Size: 438 106 563 150 7807
Fragments arranged by size:
7807 563 438 150 106
PfoI T'CCnGG_A
Cuts at: 3869 4584 5786 3869
Size: 715 1202 7147
Fragments arranged by size:
7147 1202 715
PmeI GTTT'AAAC
Cuts at: 6061 6061
Size: 9064
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1719 1751 8203 8235 1719
Size: 32 6452 32 2548
Fragments arranged by size:
6452 2548 32 32
PpuMI rG'GwC_Cy
Cuts at: 1184 3432 3627 1184
Size: 2248 195 6621
Fragments arranged by size:
6621 2248 195
PstI C_TGCA'G
Cuts at: 1010 1169 2604 6109 6794 1010
Size: 159 1435 3505 685 3280
Fragments arranged by size:
3505 3280 1435 685 159
PvuI CG_AT'CG
Cuts at: 104 104
Size: 9064
PvuII CAG'CTG
Cuts at: 346 4384 5683 5949 6487 6847 346
Size: 4038 1299 266 538 360 2563
Fragments arranged by size:
4038 2563 1299 538 360 266
RsrII CG'GwC_CG
Cuts at: 7257 7257
Size: 9064
SacI G_AGCT'C
Cuts at: 5795 6076 5795
Size: 281 8783
SalI G'TCGA_C
Cuts at: 6099 6099
Size: 9064
SanDI GG'GwC_CC
Cuts at: 3627 3627
Size: 9064
SapI GCTCTTCn'nnn_
Cuts at: 3956 5276 7085 7295 3956
Size: 1320 1809 210 5725
Fragments arranged by size:
5725 1809 1320 210
SbfI CC_TGCA'GG
Cuts at: 6109 6109
Size: 9064
ScaI AGT'ACT
Cuts at: 2952 3540 5578 8581 2952
Size: 588 2038 3003 3435
Fragments arranged by size:
3435 3003 2038 588
SfcI C'TryA_G
Cuts at: 33 1006 1165 2600 2615 4848 5213 5399 6105
Size: 973 159 1435 15 2233 365 186 706
Cuts at: 6105 6790 7785 7976 33
Size: 685 995 191 1121
Fragments arranged by size:
2233 1435 1121 995 973 706 685 365
191 186 159 15
SfoI GGC'GCC
Cuts at: 6494 6742 6494
Size: 248 8816
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 9064
SgrAI Cr'CCGG_yG
Cuts at: 5260 5260
Size: 9064
SmaI CCC'GGG
Cuts at: 6084 6084
Size: 9064
SmlI C'TyrA_G
Cuts at: 1731 3344 3485 3621 5695 6217 7132 7626 7888
Size: 1613 141 136 2074 522 915 494 262
Cuts at: 7888 1731
Size: 2907
Fragments arranged by size:
2907 2074 1613 915 522 494 262 141
136
SnaBI TAC'GTA
Cuts at: 6054 6054
Size: 9064
SpeI A'CTAG_T
Cuts at: 67 67
Size: 9064
SphI G_CATG'C
Cuts at: 6115 6615 6115
Size: 500 8564
StuI AGG'CCT
Cuts at: 3565 4286 3565
Size: 721 8343
StyI C'CwwG_G
Cuts at: 612 2235 2552 3608 4061 4287 4397 4907 5120
Size: 1623 317 1056 453 226 110 510 213
Cuts at: 5120 5345 6196 6550 612
Size: 225 851 354 3126
Fragments arranged by size:
3126 1623 1056 851 510 453 354 317
226 225 213 110
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1112 3324 4486 5961 6645 6778 7237 7448 1112
Size: 2212 1162 1475 684 133 459 211 2728
Fragments arranged by size:
2728 2212 1475 1162 684 459 211 133
TatI w'GTAC_w
Cuts at: 876 1869 2950 3538 3688 4844 5576 8579 876
Size: 993 1081 588 150 1156 732 3003 1361
Fragments arranged by size:
3003 1361 1156 1081 993 732 588 150
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 480 497 512 529 1108 1140 1451 1472 1483
Size: 17 15 17 579 32 311 21 11
Cuts at: 1483 1504 5091 5123 5216 5248 5592 5624 8581
Size: 21 3587 32 93 32 344 32 2957
Cuts at: 8581 8613 480
Size: 32 931
Fragments arranged by size:
3587 2957 931 579 344 311 93 32
32 32 32 32 21 21 17 17
15 11
Tth111I GACn'n_nGTC
Cuts at: 3237 6859 3237
Size: 3622 5442
XbaI T'CTAG_A
Cuts at: 234 6093 234
Size: 5859 3205
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4549 5364 6557 4549
Size: 815 1193 7056
Fragments arranged by size:
7056 1193 815
XmaI C'CCGG_G
Cuts at: 6082 6082
Size: 9064
XmnI GAAnn'nnTTC
Cuts at: 5578 5578
Size: 9064
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I
BtgI BtgZI BtsI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NruI NsiI NspI PciI
PflMI PfoI PmeI PpiI PpuMI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AloI ApaI AscI AvrII
BcgI BmtI BsiWI BssHII ClaI FseI FspAI HpaI
NheI NotI PacI PasI PmlI PshAI PsiI PspOMI
PspXI PsrI SacII SexAI SfiI SrfI SspI SwaI
XhoI ZraI