Restriction map for
pF1KSDB0042
(Circular) (Six-base) MAPSORT of: pF1KSDB0042.seq Check: 4427 from: 1 to: 5152
pF1KSDB0042 5152 bp
With 182 enzymes: SgfI *
June 20, 2008 10:36 ..
AccI GT'mk_AC
Cuts at: 1874 2188 1874
Size: 314 4838
Acc65I G'GTAC_C
Cuts at: 418 2166 418
Size: 1748 3404
AclI AA'CG_TT
Cuts at: 4992 4992
Size: 5152
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 914 1237 1748 2975 3407 4156 4424 914
Size: 323 511 1227 432 749 268 1642
Fragments arranged by size:
1642 1227 749 511 432 323 268
AfeI AGC'GCT
Cuts at: 1300 1300
Size: 5152
AflIII A'CryG_T
Cuts at: 2502 3608 4585 2502
Size: 1106 977 3069
Fragments arranged by size:
3069 1106 977
AgeI A'CCGG_T
Cuts at: 3540 3540
Size: 5152
AleI CACnn'nnGTG
Cuts at: 299 299
Size: 5152
AlwNI CAG_nnn'CTG
Cuts at: 1242 2130 2455 4024 1242
Size: 888 325 1569 2370
Fragments arranged by size:
2370 1569 888 325
ApaLI G'TGCA_C
Cuts at: 3922 3922
Size: 5152
ApoI r'AATT_y
Cuts at: 2154 4505 4761 2154
Size: 2351 256 2545
Fragments arranged by size:
2545 2351 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5152
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5152
AvaI C'yCGr_G
Cuts at: 2170 2170
Size: 5152
BamHI G'GATC_C
Cuts at: 2175 2175
Size: 5152
BanI G'GyrC_C
Cuts at: 320 418 2166 2580 2828 2863 320
Size: 98 1748 414 248 35 2609
Fragments arranged by size:
2609 1748 414 248 98 35
BanII G_rGCy'C
Cuts at: 662 1709 1943 2164 3194 662
Size: 1047 234 221 1030 2620
Fragments arranged by size:
2620 1047 1030 234 221
BbeI G_GCGC'C
Cuts at: 2584 2832 2584
Size: 248 4904
BbsI GAAGACnn'nnnn_
Cuts at: 1064 1324 1846 1064
Size: 260 522 4370
Fragments arranged by size:
4370 522 260
BbvCI CC'TCA_GC
Cuts at: 1408 1408
Size: 5152
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1456 1490 1581 1615 1456
Size: 34 91 34 4993
Fragments arranged by size:
4993 91 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 1777 3048 3811 5152 1777
Size: 1271 763 1341 1777
Fragments arranged by size:
1777 1341 1271 763
BclI T'GATC_A
Cuts at: 2670 2670
Size: 5152
BglII A'GATC_T
Cuts at: 954 1896 2665 4704 954
Size: 942 769 2039 1402
Fragments arranged by size:
2039 1402 942 769
BlpI GC'TnA_GC
Cuts at: 2262 2262
Size: 5152
Bme1580I G_kGCm'C
Cuts at: 318 2775 2868 3926 318
Size: 2457 93 1058 1544
Fragments arranged by size:
2457 1544 1058 93
BmrI ACTGGGnnnn_n'
Cuts at: 479 656 2000 2042 2362 2443 2778 4924 479
Size: 177 1344 42 320 81 335 2146 707
Fragments arranged by size:
2146 1344 707 335 320 177 81 42
BmtI G_CTAG'C
Cuts at: 1099 1099
Size: 5152
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 791 3517 791
Size: 2726 2426
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 277 444 747 875 1693 277
Size: 167 303 128 818 3736
Fragments arranged by size:
3736 818 303 167 128
Bpu10I CC'TnA_GC
Cuts at: 1408 1408
Size: 5152
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 815 2326 3205 3699 3997 815
Size: 1511 879 494 298 1970
Fragments arranged by size:
1970 1511 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 4856 4856
Size: 5152
BsaAI yAC'GTr
Cuts at: 2142 2503 2142
Size: 361 4791
BsaBI GATnn'nnATC
Cuts at: 6 1153 2664 4703 6
Size: 1147 1511 2039 455
Fragments arranged by size:
2039 1511 1147 455
BsaHI Gr'CG_yC
Cuts at: 2581 2829 3533 5029 2581
Size: 248 704 1496 2704
Fragments arranged by size:
2704 1496 704 248
BsaWI w'CCGG_w
Cuts at: 209 1673 2339 2860 3540 3814 3961 4372 209
Size: 1464 666 521 680 274 147 411 989
Fragments arranged by size:
1464 989 680 666 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 575 605 575
Size: 30 5122
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 175 1113 175
Size: 938 4214
BseYI C'CCAG_C
Cuts at: 1644 1977 2130 2575 3333 3912 4408 1644
Size: 333 153 445 758 579 496 2388
Fragments arranged by size:
2388 758 579 496 445 333 153
BsiEI CG_ry'CG
Cuts at: 104 1139 2692 2738 3948 104
Size: 1035 1553 46 1210 1308
Fragments arranged by size:
1553 1308 1210 1035 46
BsiHKAI G_wGCw'C
Cuts at: 1709 2164 2942 3132 3926 1709
Size: 455 778 190 794 2935
Fragments arranged by size:
2935 794 778 455 190
BsmBI CGTCTCn'nnnn_
Cuts at: 1396 1396
Size: 5152
Bsp1286I G_dGCh'C
Cuts at: 318 662 1709 1943 2164 2775 2868 2942 3132
Size: 344 1047 234 221 611 93 74 190
Cuts at: 3132 3194 3926 318
Size: 62 732 1544
Fragments arranged by size:
1544 1047 732 611 344 234 221 190
93 74 62
BspEI T'CCGG_A
Cuts at: 209 4372 209
Size: 4163 989
BspHI T'CATG_A
Cuts at: 4328 4328
Size: 5152
BspMI ACCTGCnnnn'nnnn_
Cuts at: 564 2200 2414 2716 3097 564
Size: 1636 214 302 381 2619
Fragments arranged by size:
2619 1636 381 302 214
BsrBI CCG'CTC
Cuts at: 50 648 3442 3496 4982 5147 50
Size: 598 2794 54 1486 165 55
Fragments arranged by size:
2794 1486 598 165 55 54
BsrDI GCAATG_nn'
Cuts at: 420 1551 1959 3062 420
Size: 1131 408 1103 2510
Fragments arranged by size:
2510 1131 1103 408
BsrFI r'CCGG_y
Cuts at: 3148 3329 3540 3148
Size: 181 211 4760
Fragments arranged by size:
4760 211 181
BssSI C'ACGA_G
Cuts at: 3421 3781 3421
Size: 360 4792
BstAPI GCAn_nnn'nTGC
Cuts at: 437 2061 2527 437
Size: 1624 466 3062
Fragments arranged by size:
3062 1624 466
BstBI TT'CG_AA
Cuts at: 110 110
Size: 5152
BstEII G'GTnAC_C
Cuts at: 1669 1669
Size: 5152
BstXI CCAn_nnnn'nTGG
Cuts at: 2081 2081
Size: 5152
BstYI r'GATC_y
Cuts at: 1 954 1418 1511 1896 2175 2642 2665 3000
Size: 953 464 93 385 279 467 23 335
Cuts at: 3000 3246 4260 4346 4358 4704 1
Size: 246 1014 86 12 346 449
Fragments arranged by size:
1014 953 467 464 449 385 346 335
279 246 93 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 922 922
Size: 5152
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1069 3100 3281 3444 1069
Size: 2031 181 163 2777
Fragments arranged by size:
2777 2031 181 163
BtsI GCAGTG_nn'
Cuts at: 566 2393 566
Size: 1827 3325
DraI TTT'AAA
Cuts at: 2149 2149
Size: 5152
DraIII CAC_nnn'GTG
Cuts at: 384 1074 384
Size: 690 4462
DrdI GACnn_nn'nnGTC
Cuts at: 1774 2856 3716 1774
Size: 1082 860 3210
Fragments arranged by size:
3210 1082 860
EaeI y'GGCC_r
Cuts at: 622 1946 2117 2735 2909 3300 3327 4674 622
Size: 1324 171 618 174 391 27 1347 1100
Fragments arranged by size:
1347 1324 1100 618 391 174 171 27
EagI C'GGCC_G
Cuts at: 2735 2735
Size: 5152
EarI CTCTTCn'nnn_
Cuts at: 197 1034 3173 3383 197
Size: 837 2139 210 1966
Fragments arranged by size:
2139 1966 837 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3358 3670 3816 4962 3358
Size: 312 146 1146 3548
Fragments arranged by size:
3548 1146 312 146
EcoICRI GAG'CTC
Cuts at: 1707 2162 1707
Size: 455 4697
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 277 444 747 875 914 1237 1693 1748 2975
Size: 167 303 128 39 323 456 55 1227
Cuts at: 2975 3407 4156 4424 277
Size: 432 749 268 1005
Fragments arranged by size:
1227 1005 749 456 432 323 303 268
167 128 55 39
EcoNI CCTnn'n_nnAGG
Cuts at: 1501 1664 1501
Size: 163 4989
EcoO109I rG'GnC_Cy
Cuts at: 1445 2289 1445
Size: 844 4308
EcoRI G'AATT_C
Cuts at: 2154 2154
Size: 5152
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3169 3169
Size: 5152
FspI TGC'GCA
Cuts at: 2931 2931
Size: 5152
HaeII r_GCGC'y
Cuts at: 1302 2584 2832 3856 4835 1302
Size: 1282 248 1024 979 1619
Fragments arranged by size:
1619 1282 1024 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 575 607 711 743 745 777 804 836 1833
Size: 32 104 32 2 32 27 32 997
Cuts at: 1833 1865 2992 3022 3024 3054 575
Size: 32 1127 30 2 30 2673
Fragments arranged by size:
2673 1127 997 104 32 32 32 32
32 30 30 27 2 2
HincII GTy'rAC
Cuts at: 2189 2189
Size: 5152
HindIII A'AGCT_T
Cuts at: 1531 1531
Size: 5152
KasI G'GCGC_C
Cuts at: 2580 2828 2580
Size: 248 4904
KpnI G_GTAC'C
Cuts at: 422 2170 422
Size: 1748 3404
MluI A'CGCG_T
Cuts at: 4585 4585
Size: 5152
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 110 1644 3823 4007 110
Size: 1534 2179 184 1255
Fragments arranged by size:
2179 1534 1255 184
MscI TGG'CCA
Cuts at: 624 1948 2119 2911 624
Size: 1324 171 792 2865
Fragments arranged by size:
2865 1324 792 171
MslI CAynn'nnrTG
Cuts at: 299 983 1189 3139 3266 299
Size: 684 206 1950 127 2185
Fragments arranged by size:
2185 1950 684 206 127
MspA1I CmG'CkG
Cuts at: 745 1272 2261 2575 2935 3950 4195 745
Size: 527 989 314 360 1015 245 1702
Fragments arranged by size:
1702 1015 989 527 360 314 245
NaeI GCC'GGC
Cuts at: 3331 3331
Size: 5152
NarI GG'CG_CC
Cuts at: 2581 2829 2581
Size: 248 4904
NdeI CA'TA_TG
Cuts at: 1311 1907 1311
Size: 596 4556
NgoMIV G'CCGG_C
Cuts at: 3329 3329
Size: 5152
NheI G'CTAG_C
Cuts at: 1095 1095
Size: 5152
NruI TCG'CGA
Cuts at: 3513 3513
Size: 5152
NspI r_CATG'y
Cuts at: 1113 2203 2703 3612 1113
Size: 1090 500 909 2653
Fragments arranged by size:
2653 1090 909 500
PasI CC'CwG_GG
Cuts at: 2074 2074
Size: 5152
PciI A'CATG_T
Cuts at: 3608 3608
Size: 5152
PflMI CCAn_nnn'nTGG
Cuts at: 1242 1272 1242
Size: 30 5122
PfoI T'CCnGG_A
Cuts at: 763 1525 763
Size: 762 4390
PmeI GTTT'AAAC
Cuts at: 2149 2149
Size: 5152
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4291 4323 4291
Size: 32 5120
PpuMI rG'GwC_Cy
Cuts at: 1445 1445
Size: 5152
PstI C_TGCA'G
Cuts at: 561 1454 2102 2197 2882 561
Size: 893 648 95 685 2831
Fragments arranged by size:
2831 893 685 648 95
PvuI CG_AT'CG
Cuts at: 104 1139 104
Size: 1035 4117
PvuII CAG'CTG
Cuts at: 745 1272 2575 2935 745
Size: 527 1303 360 2962
Fragments arranged by size:
2962 1303 527 360
RsrII CG'GwC_CG
Cuts at: 3345 3345
Size: 5152
SacI G_AGCT'C
Cuts at: 1709 2164 1709
Size: 455 4697
SalI G'TCGA_C
Cuts at: 2187 2187
Size: 5152
SapI GCTCTTCn'nnn_
Cuts at: 3173 3383 3173
Size: 210 4942
SbfI CC_TGCA'GG
Cuts at: 2197 2197
Size: 5152
ScaI AGT'ACT
Cuts at: 4669 4669
Size: 5152
SexAI A'CCwGG_T
Cuts at: 380 380
Size: 5152
SfcI C'TryA_G
Cuts at: 33 190 297 557 1158 1450 1900 2098 2193
Size: 157 107 260 601 292 450 198 95
Cuts at: 2193 2878 3873 4064 33
Size: 685 995 191 1121
Fragments arranged by size:
1121 995 685 601 450 292 260 198
191 157 107 95
SfoI GGC'GCC
Cuts at: 2582 2830 2582
Size: 248 4904
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5152
SmaI CCC'GGG
Cuts at: 2172 2172
Size: 5152
SmlI C'TyrA_G
Cuts at: 830 2305 3220 3714 3976 830
Size: 1475 915 494 262 2006
Fragments arranged by size:
2006 1475 915 494 262
SnaBI TAC'GTA
Cuts at: 2142 2142
Size: 5152
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5152
SphI G_CATG'C
Cuts at: 1113 2203 2703 1113
Size: 1090 500 3562
Fragments arranged by size:
3562 1090 500
StuI AGG'CCT
Cuts at: 927 1022 927
Size: 95 5057
StyI C'CwwG_G
Cuts at: 443 992 1347 1497 2284 2638 443
Size: 549 355 150 787 354 2957
Fragments arranged by size:
2957 787 549 355 354 150
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 601 1969 2733 2866 3325 3536 601
Size: 1368 764 133 459 211 2217
Fragments arranged by size:
2217 1368 764 459 211 133
TatI w'GTAC_w
Cuts at: 935 4667 935
Size: 3732 1420
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1371 1403 4669 4701 1371
Size: 32 3266 32 1822
Fragments arranged by size:
3266 1822 32 32
Tth111I GACn'n_nGTC
Cuts at: 2947 2947
Size: 5152
XbaI T'CTAG_A
Cuts at: 2181 2181
Size: 5152
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2127 2645 2127
Size: 518 4634
XmaI C'CCGG_G
Cuts at: 2170 2170
Size: 5152
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglII
BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NdeI NgoMIV
NheI NruI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SnaBI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AlfI AloI ApaI AscI
AvrII BaeI BfrBI BglI BmgBI BsgI BsiWI BsmI
BsrGI BssHII BstZ17I BtgI ClaI CspCI EcoRV FseI
FspAI HpaI MfeI NcoI NotI NsiI PacI PmlI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SfiI
SgrAI SrfI SspI SwaI XhoI XmnI ZraI