Restriction map for
pF1KSDB0037
(Circular) (Six-base) MAPSORT of: pF1KSDB0037.seq Check: 1612 from: 1 to: 4552
pF1KSDB0037 4552 bp
With 182 enzymes: SgfI *
June 20, 2008 10:36 ..
AatII G_ACGT'C
Cuts at: 779 779
Size: 4552
AccI GT'mk_AC
Cuts at: 1415 1588 1415
Size: 173 4379
Acc65I G'GTAC_C
Cuts at: 1272 1566 1272
Size: 294 4258
AclI AA'CG_TT
Cuts at: 4392 4392
Size: 4552
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 730 901 2375 2807 3556 3824 730
Size: 171 1474 432 749 268 1458
Fragments arranged by size:
1474 1458 749 432 268 171
AflIII A'CryG_T
Cuts at: 1902 3008 3985 1902
Size: 1106 977 2469
Fragments arranged by size:
2469 1106 977
AgeI A'CCGG_T
Cuts at: 2940 2940
Size: 4552
AleI CACnn'nnGTG
Cuts at: 1094 1094
Size: 4552
AlwNI CAG_nnn'CTG
Cuts at: 1855 3424 1855
Size: 1569 2983
ApaLI G'TGCA_C
Cuts at: 3322 3322
Size: 4552
ApoI r'AATT_y
Cuts at: 655 675 1502 1554 3905 4161 655
Size: 20 827 52 2351 256 1046
Fragments arranged by size:
2351 1046 827 256 52 20
AseI AT'TA_AT
Cuts at: 20 20
Size: 4552
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4552
AvaI C'yCGr_G
Cuts at: 860 1570 860
Size: 710 3842
BamHI G'GATC_C
Cuts at: 1364 1575 1364
Size: 211 4341
BanI G'GyrC_C
Cuts at: 241 1272 1566 1980 2228 2263 241
Size: 1031 294 414 248 35 2530
Fragments arranged by size:
2530 1031 414 294 248 35
BanII G_rGCy'C
Cuts at: 1348 1564 2594 1348
Size: 216 1030 3306
Fragments arranged by size:
3306 1030 216
BbeI G_GCGC'C
Cuts at: 1984 2232 1984
Size: 248 4304
BbsI GAAGACnn'nnnn_
Cuts at: 771 1256 771
Size: 485 4067
BbvCI CC'TCA_GC
Cuts at: 1128 1128
Size: 4552
BciVI GTATCCnnnnn_n'
Cuts at: 2448 3211 4552 2448
Size: 763 1341 2448
Fragments arranged by size:
2448 1341 763
BclI T'GATC_A
Cuts at: 2070 2070
Size: 4552
BglII A'GATC_T
Cuts at: 935 2065 4104 935
Size: 1130 2039 1383
Fragments arranged by size:
2039 1383 1130
BlpI GC'TnA_GC
Cuts at: 1662 1662
Size: 4552
Bme1580I G_kGCm'C
Cuts at: 962 2175 2268 3326 962
Size: 1213 93 1058 2188
Fragments arranged by size:
2188 1213 1058 93
BmgBI CAC'GTC
Cuts at: 786 1165 786
Size: 379 4173
BmrI ACTGGGnnnn_n'
Cuts at: 1762 1843 2178 4324 1762
Size: 81 335 2146 1990
Fragments arranged by size:
2146 1990 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2917 2917
Size: 4552
Bpu10I CC'TnA_GC
Cuts at: 1128 1128
Size: 4552
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1726 2605 3099 3397 1726
Size: 879 494 298 2881
Fragments arranged by size:
2881 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 532 766 4256 532
Size: 234 3490 828
Fragments arranged by size:
3490 828 234
BsaAI yAC'GTr
Cuts at: 1542 1903 1542
Size: 361 4191
BsaBI GATnn'nnATC
Cuts at: 6 142 395 1139 2064 4103 6
Size: 136 253 744 925 2039 455
Fragments arranged by size:
2039 925 744 455 253 136
BsaHI Gr'CG_yC
Cuts at: 776 1981 2229 2933 4429 776
Size: 1205 248 704 1496 899
Fragments arranged by size:
1496 1205 899 704 248
BsaWI w'CCGG_w
Cuts at: 1739 2260 2940 3214 3361 3772 1739
Size: 521 680 274 147 411 2519
Fragments arranged by size:
2519 680 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1081 1081
Size: 4552
BseYI C'CCAG_C
Cuts at: 328 1975 2733 3312 3808 328
Size: 1647 758 579 496 1072
Fragments arranged by size:
1647 1072 758 579 496
BsiEI CG_ry'CG
Cuts at: 104 2092 2138 3348 104
Size: 1988 46 1210 1308
Fragments arranged by size:
1988 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 1564 2342 2532 3326 1564
Size: 778 190 794 2790
Fragments arranged by size:
2790 794 778 190
BsmI GAATG_Cn'
Cuts at: 623 623
Size: 4552
Bsp1286I G_dGCh'C
Cuts at: 962 1348 1564 2175 2268 2342 2532 2594 3326
Size: 386 216 611 93 74 190 62 732
Cuts at: 3326 962
Size: 2188
Fragments arranged by size:
2188 732 611 386 216 190 93 74
62
BspEI T'CCGG_A
Cuts at: 3772 3772
Size: 4552
BspHI T'CATG_A
Cuts at: 3728 3728
Size: 4552
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1600 1814 2116 2497 1600
Size: 214 302 381 3655
Fragments arranged by size:
3655 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2842 2896 4382 4547 50
Size: 2792 54 1486 165 55
Fragments arranged by size:
2792 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 205 872 2462 205
Size: 667 1590 2295
Fragments arranged by size:
2295 1590 667
BsrFI r'CCGG_y
Cuts at: 178 2548 2729 2940 178
Size: 2370 181 211 1790
Fragments arranged by size:
2370 1790 211 181
BssSI C'ACGA_G
Cuts at: 2821 3181 2821
Size: 360 4192
BstAPI GCAn_nnn'nTGC
Cuts at: 1927 1927
Size: 4552
BstBI TT'CG_AA
Cuts at: 110 110
Size: 4552
BstYI r'GATC_y
Cuts at: 1 935 1364 1398 1575 2042 2065 2400 2646
Size: 934 429 34 177 467 23 335 246
Cuts at: 2646 3660 3746 3758 4104 1
Size: 1014 86 12 346 449
Fragments arranged by size:
1014 934 467 449 429 346 335 246
177 86 34 23 12
BtgI C'CryG_G
Cuts at: 125 125
Size: 4552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1126 2500 2681 2844 1126
Size: 1374 181 163 2834
Fragments arranged by size:
2834 1374 181 163
BtsI GCAGTG_nn'
Cuts at: 1793 1793
Size: 4552
DraI TTT'AAA
Cuts at: 497 1549 497
Size: 1052 3500
DrdI GACnn_nn'nnGTC
Cuts at: 2256 3116 2256
Size: 860 3692
EaeI y'GGCC_r
Cuts at: 128 176 2135 2309 2700 2727 4074 128
Size: 48 1959 174 391 27 1347 606
Fragments arranged by size:
1959 1347 606 391 174 48 27
EagI C'GGCC_G
Cuts at: 2135 2135
Size: 4552
EarI CTCTTCn'nnn_
Cuts at: 449 1031 2573 2783 449
Size: 582 1542 210 2218
Fragments arranged by size:
2218 1542 582 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 821 2758 3070 3216 4362 821
Size: 1937 312 146 1146 1011
Fragments arranged by size:
1937 1146 1011 312 146
EcoICRI GAG'CTC
Cuts at: 1562 1562
Size: 4552
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 730 901 2375 2807 3556 3824 730
Size: 171 1474 432 749 268 1458
Fragments arranged by size:
1474 1458 749 432 268 171
EcoNI CCTnn'n_nnAGG
Cuts at: 278 278
Size: 4552
EcoO109I rG'GnC_Cy
Cuts at: 1689 1689
Size: 4552
EcoRI G'AATT_C
Cuts at: 1554 1554
Size: 4552
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1138 2569 1138
Size: 1431 3121
FspI TGC'GCA
Cuts at: 2331 2331
Size: 4552
HaeII r_GCGC'y
Cuts at: 1984 2232 3256 4235 1984
Size: 248 1024 979 2301
Fragments arranged by size:
2301 1024 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1014 1046 1433 1456 1465 1488 2392 2422 2424
Size: 32 387 23 9 23 904 30 2
Cuts at: 2424 2454 1014
Size: 30 3112
Fragments arranged by size:
3112 904 387 32 30 30 23 23
9 2
HincII GTy'rAC
Cuts at: 1589 1589
Size: 4552
KasI G'GCGC_C
Cuts at: 1980 2228 1980
Size: 248 4304
KpnI G_GTAC'C
Cuts at: 1276 1570 1276
Size: 294 4258
MfeI C'AATT_G
Cuts at: 683 683
Size: 4552
MluI A'CGCG_T
Cuts at: 3985 3985
Size: 4552
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 440 3223 3407 440
Size: 2783 184 1585
Fragments arranged by size:
2783 1585 184
MscI TGG'CCA
Cuts at: 130 2311 130
Size: 2181 2371
MslI CAynn'nnrTG
Cuts at: 547 1094 2539 2666 547
Size: 547 1445 127 2433
Fragments arranged by size:
2433 1445 547 127
MspA1I CmG'CkG
Cuts at: 1661 1975 2335 3350 3595 1661
Size: 314 360 1015 245 2618
Fragments arranged by size:
2618 1015 360 314 245
NaeI GCC'GGC
Cuts at: 180 2731 180
Size: 2551 2001
NarI GG'CG_CC
Cuts at: 1981 2229 1981
Size: 248 4304
NcoI C'CATG_G
Cuts at: 125 125
Size: 4552
NdeI CA'TA_TG
Cuts at: 948 948
Size: 4552
NgoMIV G'CCGG_C
Cuts at: 178 2729 178
Size: 2551 2001
NruI TCG'CGA
Cuts at: 2913 2913
Size: 4552
NspI r_CATG'y
Cuts at: 1603 2103 3012 1603
Size: 500 909 3143
Fragments arranged by size:
3143 909 500
PciI A'CATG_T
Cuts at: 3008 3008
Size: 4552
PflMI CCAn_nnn'nTGG
Cuts at: 789 789
Size: 4552
PmeI GTTT'AAAC
Cuts at: 1549 1549
Size: 4552
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3691 3723 3691
Size: 32 4520
PstI C_TGCA'G
Cuts at: 1597 2282 1597
Size: 685 3867
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4552
PvuII CAG'CTG
Cuts at: 1975 2335 1975
Size: 360 4192
RsrII CG'GwC_CG
Cuts at: 2745 2745
Size: 4552
SacI G_AGCT'C
Cuts at: 1564 1564
Size: 4552
SalI G'TCGA_C
Cuts at: 1587 1587
Size: 4552
SapI GCTCTTCn'nnn_
Cuts at: 2573 2783 2573
Size: 210 4342
SbfI CC_TGCA'GG
Cuts at: 1597 1597
Size: 4552
ScaI AGT'ACT
Cuts at: 922 4069 922
Size: 3147 1405
SfcI C'TryA_G
Cuts at: 33 1412 1593 2278 3273 3464 33
Size: 1379 181 685 995 191 1121
Fragments arranged by size:
1379 1121 995 685 191 181
SfoI GGC'GCC
Cuts at: 1982 2230 1982
Size: 248 4304
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4552
SmaI CCC'GGG
Cuts at: 1572 1572
Size: 4552
SmlI C'TyrA_G
Cuts at: 1705 2620 3114 3376 1705
Size: 915 494 262 2881
Fragments arranged by size:
2881 915 494 262
SnaBI TAC'GTA
Cuts at: 1542 1542
Size: 4552
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4552
SphI G_CATG'C
Cuts at: 1603 2103 1603
Size: 500 4052
StyI C'CwwG_G
Cuts at: 125 1684 2038 125
Size: 1559 354 2639
Fragments arranged by size:
2639 1559 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2133 2266 2725 2936 2133
Size: 133 459 211 3749
Fragments arranged by size:
3749 459 211 133
TatI w'GTAC_w
Cuts at: 920 4067 920
Size: 3147 1405
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1341 1373 4069 4101 1341
Size: 32 2696 32 1792
Fragments arranged by size:
2696 1792 32 32
Tth111I GACn'n_nGTC
Cuts at: 2347 2347
Size: 4552
XbaI T'CTAG_A
Cuts at: 816 1581 816
Size: 765 3787
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2045 2045
Size: 4552
XmaI C'CCGG_G
Cuts at: 1570 1570
Size: 4552
ZraI GAC'GTC
Cuts at: 777 777
Size: 4552
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglII BlpI
Bme1580I BmgBI BmrI BplI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NruI NspI PciI PflMI PmeI
PpiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SnaBI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AlfI AloI ApaI AscI
AvrII BaeI BcgI BfrBI BglI BmtI BpmI BsaXI
BsgI BsiWI BsmBI BsrGI BssHII BstEII BstXI BstZ17I
Bsu36I ClaI CspCI DraIII EcoRV FseI FspAI HindIII
HpaI NheI NotI NsiI PacI PasI PfoI PmlI
PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SrfI SspI StuI SwaI XhoI
XmnI