Restriction map for
pF1KSDB0036
(Circular) (Six-base) MAPSORT of: pF1KSDB0036.seq Check: 2498 from: 1 to: 5072
pF1KSDB0036 5072 bp
With 182 enzymes: SgfI *
June 20, 2008 10:36 ..
AccI GT'mk_AC
Cuts at: 487 2110 487
Size: 1623 3449
Acc65I G'GTAC_C
Cuts at: 2088 2088
Size: 5072
AclI AA'CG_TT
Cuts at: 4912 4912
Size: 5072
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1445 1532 2897 3329 4076 4344 1445
Size: 87 1365 432 747 268 2173
Fragments arranged by size:
2173 1365 747 432 268 87
AflII C'TTAA_G
Cuts at: 441 441
Size: 5072
AflIII A'CryG_T
Cuts at: 428 482 2424 3528 4505 428
Size: 54 1942 1104 977 995
Fragments arranged by size:
1942 1104 995 977 54
AgeI A'CCGG_T
Cuts at: 3460 3460
Size: 5072
AleI CACnn'nnGTG
Cuts at: 565 565
Size: 5072
AlwNI CAG_nnn'CTG
Cuts at: 141 2377 3944 141
Size: 2236 1567 1269
Fragments arranged by size:
2236 1567 1269
ApaI G_GGCC'C
Cuts at: 236 236
Size: 5072
ApaLI G'TGCA_C
Cuts at: 267 3842 267
Size: 3575 1497
ApoI r'AATT_y
Cuts at: 1940 2076 4425 4681 1940
Size: 136 2349 256 2331
Fragments arranged by size:
2349 2331 256 136
AseI AT'TA_AT
Cuts at: 20 773 20
Size: 753 4319
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5072
AvaI C'yCGr_G
Cuts at: 2092 2092
Size: 5072
BamHI G'GATC_C
Cuts at: 2097 2097
Size: 5072
BanI G'GyrC_C
Cuts at: 1884 2088 2502 2750 2785 1884
Size: 204 414 248 35 4171
Fragments arranged by size:
4171 414 248 204 35
BanII G_rGCy'C
Cuts at: 236 2086 3116 236
Size: 1850 1030 2192
Fragments arranged by size:
2192 1850 1030
BbeI G_GCGC'C
Cuts at: 2506 2754 2506
Size: 248 4824
BbsI GAAGACnn'nnnn_
Cuts at: 342 937 1037 342
Size: 595 100 4377
Fragments arranged by size:
4377 595 100
BbvCI CC'TCA_GC
Cuts at: 1215 1215
Size: 5072
BciVI GTATCCnnnnn_n'
Cuts at: 201 1218 2970 3731 5072 201
Size: 1017 1752 761 1341 201
Fragments arranged by size:
1752 1341 1017 761 201
BclI T'GATC_A
Cuts at: 2592 2592
Size: 5072
BglII A'GATC_T
Cuts at: 2587 4624 2587
Size: 2037 3035
BlpI GC'TnA_GC
Cuts at: 2184 2184
Size: 5072
Bme1580I G_kGCm'C
Cuts at: 236 271 2697 2790 3846 236
Size: 35 2426 93 1056 1462
Fragments arranged by size:
2426 1462 1056 93 35
BmrI ACTGGGnnnn_n'
Cuts at: 1832 2284 2365 2700 4844 1832
Size: 452 81 335 2144 2060
Fragments arranged by size:
2144 2060 452 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3439 3439
Size: 5072
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1511 1511
Size: 5072
Bpu10I CC'TnA_GC
Cuts at: 1215 1215
Size: 5072
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2248 3127 3619 3917 2248
Size: 879 492 298 3403
Fragments arranged by size:
3403 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 2004 4776 2004
Size: 2772 2300
BsaAI yAC'GTr
Cuts at: 2064 2425 2064
Size: 361 4711
BsaBI GATnn'nnATC
Cuts at: 6 2586 4623 6
Size: 2580 2037 455
Fragments arranged by size:
2580 2037 455
BsaHI Gr'CG_yC
Cuts at: 2503 2751 3453 4949 2503
Size: 248 702 1496 2626
Fragments arranged by size:
2626 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2261 2782 3460 3734 3881 4292 2261
Size: 521 678 274 147 411 3041
Fragments arranged by size:
3041 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1373 1403 1509 1539 1373
Size: 30 106 30 4906
Fragments arranged by size:
4906 106 30 30
BseYI C'CCAG_C
Cuts at: 164 2497 3255 3832 4328 164
Size: 2333 758 577 496 908
Fragments arranged by size:
2333 908 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1220 1220
Size: 5072
BsiEI CG_ry'CG
Cuts at: 104 2614 2660 3868 104
Size: 2510 46 1208 1308
Fragments arranged by size:
2510 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 271 2046 2086 2864 3054 3846 271
Size: 1775 40 778 190 792 1497
Fragments arranged by size:
1775 1497 792 778 190 40
BsmI GAATG_Cn'
Cuts at: 1875 1875
Size: 5072
Bsp1286I G_dGCh'C
Cuts at: 236 271 2046 2086 2697 2790 2864 3054 3116
Size: 35 1775 40 611 93 74 190 62
Cuts at: 3116 3846 236
Size: 730 1462
Fragments arranged by size:
1775 1462 730 611 190 93 74 62
40 35
BspEI T'CCGG_A
Cuts at: 4292 4292
Size: 5072
BspHI T'CATG_A
Cuts at: 1497 2006 4248 1497
Size: 509 2242 2321
Fragments arranged by size:
2321 2242 509
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2122 2336 2638 3019 2122
Size: 214 302 381 4175
Fragments arranged by size:
4175 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3364 3418 4902 5067 50
Size: 3314 54 1484 165 55
Fragments arranged by size:
3314 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 687 2984 687
Size: 2297 2775
BsrFI r'CCGG_y
Cuts at: 178 3070 3251 3460 178
Size: 2892 181 209 1790
Fragments arranged by size:
2892 1790 209 181
BsrGI T'GTAC_A
Cuts at: 1338 1338
Size: 5072
BssSI C'ACGA_G
Cuts at: 220 3343 3701 220
Size: 3123 358 1591
Fragments arranged by size:
3123 1591 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2449 2449
Size: 5072
BstBI TT'CG_AA
Cuts at: 110 3433 110
Size: 3323 1749
BstXI CCAn_nnnn'nTGG
Cuts at: 1746 1746
Size: 5072
BstYI r'GATC_y
Cuts at: 1 1828 2097 2564 2587 2922 3168 4180 4266
Size: 1827 269 467 23 335 246 1012 86
Cuts at: 4266 4278 4624 1
Size: 12 346 449
Fragments arranged by size:
1827 1012 467 449 346 335 269 246
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3022 3203 3366 3022
Size: 181 163 4728
Fragments arranged by size:
4728 181 163
BtsI GCAGTG_nn'
Cuts at: 155 1046 2315 155
Size: 891 1269 2912
Fragments arranged by size:
2912 1269 891
DraI TTT'AAA
Cuts at: 518 1139 2071 518
Size: 621 932 3519
Fragments arranged by size:
3519 932 621
DrdI GACnn_nn'nnGTC
Cuts at: 2778 3636 2778
Size: 858 4214
EaeI y'GGCC_r
Cuts at: 132 2657 2831 3222 3249 4594 132
Size: 2525 174 391 27 1345 610
Fragments arranged by size:
2525 1345 610 391 174 27
EagI C'GGCC_G
Cuts at: 2657 2657
Size: 5072
EarI CTCTTCn'nnn_
Cuts at: 565 3095 3305 565
Size: 2530 210 2332
Fragments arranged by size:
2530 2332 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3280 3590 3736 4882 3280
Size: 310 146 1146 3470
Fragments arranged by size:
3470 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2084 2084
Size: 5072
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1445 1511 1532 2897 3329 4076 4344 1445
Size: 66 21 1365 432 747 268 2173
Fragments arranged by size:
2173 1365 747 432 268 66 21
EcoNI CCTnn'n_nnAGG
Cuts at: 928 2036 928
Size: 1108 3964
EcoO109I rG'GnC_Cy
Cuts at: 232 933 2211 232
Size: 701 1278 3093
Fragments arranged by size:
3093 1278 701
EcoRI G'AATT_C
Cuts at: 1940 2076 1940
Size: 136 4936
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 841 3091 841
Size: 2250 2822
FspI TGC'GCA
Cuts at: 2853 2853
Size: 5072
HaeII r_GCGC'y
Cuts at: 1152 2506 2754 3776 4755 1152
Size: 1354 248 1022 979 1469
Fragments arranged by size:
1469 1354 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1479 1511 1558 1590 2914 2944 2946 2976 1479
Size: 32 47 32 1324 30 2 30 3575
Fragments arranged by size:
3575 1324 47 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1352 1566 1801 1820 2111 1352
Size: 214 235 19 291 4313
Fragments arranged by size:
4313 291 235 214 19
HindIII A'AGCT_T
Cuts at: 1133 1407 1133
Size: 274 4798
HpaI GTT'AAC
Cuts at: 1820 1820
Size: 5072
KasI G'GCGC_C
Cuts at: 2502 2750 2502
Size: 248 4824
KpnI G_GTAC'C
Cuts at: 2092 2092
Size: 5072
MluI A'CGCG_T
Cuts at: 4505 4505
Size: 5072
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 761 876 1491 3743 3927 761
Size: 115 615 2252 184 1906
Fragments arranged by size:
2252 1906 615 184 115
MscI TGG'CCA
Cuts at: 2833 2833
Size: 5072
MslI CAynn'nnrTG
Cuts at: 565 3061 3188 565
Size: 2496 127 2449
Fragments arranged by size:
2496 2449 127
MspA1I CmG'CkG
Cuts at: 1009 1552 2183 2497 2857 3870 4115 1009
Size: 543 631 314 360 1013 245 1966
Fragments arranged by size:
1966 1013 631 543 360 314 245
NaeI GCC'GGC
Cuts at: 3253 3253
Size: 5072
NarI GG'CG_CC
Cuts at: 2503 2751 2503
Size: 248 4824
NgoMIV G'CCGG_C
Cuts at: 3251 3251
Size: 5072
NspI r_CATG'y
Cuts at: 486 2125 2625 3532 486
Size: 1639 500 907 2026
Fragments arranged by size:
2026 1639 907 500
PciI A'CATG_T
Cuts at: 482 3528 482
Size: 3046 2026
PmeI GTTT'AAAC
Cuts at: 2071 2071
Size: 5072
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4211 4243 4211
Size: 32 5040
PspOMI G'GGCC_C
Cuts at: 232 232
Size: 5072
PstI C_TGCA'G
Cuts at: 1197 2119 2804 1197
Size: 922 685 3465
Fragments arranged by size:
3465 922 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5072
PvuII CAG'CTG
Cuts at: 1009 1552 2497 2857 1009
Size: 543 945 360 3224
Fragments arranged by size:
3224 945 543 360
RsrII CG'GwC_CG
Cuts at: 147 3267 147
Size: 3120 1952
SacI G_AGCT'C
Cuts at: 2086 2086
Size: 5072
SalI G'TCGA_C
Cuts at: 2109 2109
Size: 5072
SapI GCTCTTCn'nnn_
Cuts at: 3095 3305 3095
Size: 210 4862
SbfI CC_TGCA'GG
Cuts at: 2119 2119
Size: 5072
ScaI AGT'ACT
Cuts at: 4589 4589
Size: 5072
SexAI A'CCwGG_T
Cuts at: 1803 1803
Size: 5072
SfcI C'TryA_G
Cuts at: 33 1004 1193 2115 2800 3793 3984 33
Size: 971 189 922 685 993 191 1121
Fragments arranged by size:
1121 993 971 922 685 191 189
SfoI GGC'GCC
Cuts at: 2504 2752 2504
Size: 248 4824
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5072
SmaI CCC'GGG
Cuts at: 2094 2094
Size: 5072
SmlI C'TyrA_G
Cuts at: 441 2227 3142 3634 3896 441
Size: 1786 915 492 262 1617
Fragments arranged by size:
1786 1617 915 492 262
SnaBI TAC'GTA
Cuts at: 2064 2064
Size: 5072
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5072
SphI G_CATG'C
Cuts at: 2125 2625 2125
Size: 500 4572
SspI AAT'ATT
Cuts at: 790 790
Size: 5072
StyI C'CwwG_G
Cuts at: 977 2206 2560 977
Size: 1229 354 3489
Fragments arranged by size:
3489 1229 354
SwaI ATTT'AAAT
Cuts at: 518 518
Size: 5072
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 164 330 1346 2655 2788 3247 3456 164
Size: 166 1016 1309 133 459 209 1780
Fragments arranged by size:
1780 1309 1016 459 209 166 133
TatI w'GTAC_w
Cuts at: 1338 1372 4587 1338
Size: 34 3215 1823
Fragments arranged by size:
3215 1823 34
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1509 1541 4589 4621 1509
Size: 32 3048 32 1960
Fragments arranged by size:
3048 1960 32 32
Tth111I GACn'n_nGTC
Cuts at: 2869 2869
Size: 5072
XbaI T'CTAG_A
Cuts at: 2103 2103
Size: 5072
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1862 2567 1862
Size: 705 4367
XmaI C'CCGG_G
Cuts at: 2092 2092
Size: 5072
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AleI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NspI PciI PmeI
PpiI PspOMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SnaBI SpeI SphI SspI StyI SwaI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AhdI AlfI AloI AscI AvrII
BaeI BcgI BfrBI BglI BmgBI BmtI BseRI BsiWI
BsmBI BssHII BstEII BstZ17I Bsu36I BtgI ClaI CspCI
DraIII EcoRV FseI FspAI MfeI NcoI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PfoI PmlI
PpuMI PshAI PsiI PspXI PsrI SacII SanDI SfiI
SgrAI SrfI StuI XhoI XmnI ZraI