Restriction map for
pF1KSDB0032
(Circular) (Six-base) MAPSORT of: pF1KSDB0032.seq Check: 8950 from: 1 to: 4576
pF1KSDB0032 4576 bp
With 182 enzymes: SgfI *
June 20, 2008 10:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1047 1047
Size: 4576
AatII G_ACGT'C
Cuts at: 1041 1041
Size: 4576
AccI GT'mk_AC
Cuts at: 1562 1612 1562
Size: 50 4526
Acc65I G'GTAC_C
Cuts at: 1590 1590
Size: 4576
AclI AA'CG_TT
Cuts at: 4416 4416
Size: 4576
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 700 1156 1368 1481 2399 2831 3580 3848 700
Size: 456 212 113 918 432 749 268 1428
Fragments arranged by size:
1428 918 749 456 432 268 212 113
AflIII A'CryG_T
Cuts at: 1926 3032 4009 1926
Size: 1106 977 2493
Fragments arranged by size:
2493 1106 977
AgeI A'CCGG_T
Cuts at: 2964 2964
Size: 4576
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 239 239
Size: 4576
AlwNI CAG_nnn'CTG
Cuts at: 1879 3448 1879
Size: 1569 3007
ApaLI G'TGCA_C
Cuts at: 3346 3346
Size: 4576
ApoI r'AATT_y
Cuts at: 496 971 1045 1262 1578 3929 4185 496
Size: 475 74 217 316 2351 256 887
Fragments arranged by size:
2351 887 475 316 256 217 74
AseI AT'TA_AT
Cuts at: 20 20
Size: 4576
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4576
AvaI C'yCGr_G
Cuts at: 752 1594 752
Size: 842 3734
BamHI G'GATC_C
Cuts at: 1599 1599
Size: 4576
BanI G'GyrC_C
Cuts at: 1590 2004 2252 2287 1590
Size: 414 248 35 3879
Fragments arranged by size:
3879 414 248 35
BanII G_rGCy'C
Cuts at: 1588 2618 1588
Size: 1030 3546
BbeI G_GCGC'C
Cuts at: 2008 2256 2008
Size: 248 4328
BbsI GAAGACnn'nnnn_
Cuts at: 1195 1195
Size: 4576
BciVI GTATCCnnnnn_n'
Cuts at: 2472 3235 4576 2472
Size: 763 1341 2472
Fragments arranged by size:
2472 1341 763
BclI T'GATC_A
Cuts at: 2094 2094
Size: 4576
BglII A'GATC_T
Cuts at: 461 589 2089 4128 461
Size: 128 1500 2039 909
Fragments arranged by size:
2039 1500 909 128
BlpI GC'TnA_GC
Cuts at: 212 1686 212
Size: 1474 3102
Bme1580I G_kGCm'C
Cuts at: 2199 2292 3350 2199
Size: 93 1058 3425
Fragments arranged by size:
3425 1058 93
BmrI ACTGGGnnnn_n'
Cuts at: 445 1786 1867 2202 4348 445
Size: 1341 81 335 2146 673
Fragments arranged by size:
2146 1341 673 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2941 2941
Size: 4576
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1490 1490
Size: 4576
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1204 1750 2629 3123 3421 1204
Size: 546 879 494 298 2359
Fragments arranged by size:
2359 879 546 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 4280 4280
Size: 4576
BsaAI yAC'GTr
Cuts at: 1566 1927 1566
Size: 361 4215
BsaBI GATnn'nnATC
Cuts at: 6 1286 2088 4127 6
Size: 1280 802 2039 455
Fragments arranged by size:
2039 1280 802 455
BsaHI Gr'CG_yC
Cuts at: 1038 2005 2253 2957 4453 1038
Size: 967 248 704 1496 1161
Fragments arranged by size:
1496 1161 967 704 248
BsaWI w'CCGG_w
Cuts at: 1763 2284 2964 3238 3385 3796 1763
Size: 521 680 274 147 411 2543
Fragments arranged by size:
2543 680 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 584 584
Size: 4576
BseYI C'CCAG_C
Cuts at: 1999 2757 3336 3832 1999
Size: 758 579 496 2743
Fragments arranged by size:
2743 758 579 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 175 175
Size: 4576
BsiEI CG_ry'CG
Cuts at: 104 2116 2162 3372 104
Size: 2012 46 1210 1308
Fragments arranged by size:
2012 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 1588 2366 2556 3350 1588
Size: 778 190 794 2814
Fragments arranged by size:
2814 794 778 190
BsmI GAATG_Cn'
Cuts at: 1034 1034
Size: 4576
Bsp1286I G_dGCh'C
Cuts at: 1588 2199 2292 2366 2556 2618 3350 1588
Size: 611 93 74 190 62 732 2814
Fragments arranged by size:
2814 732 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3796 3796
Size: 4576
BspHI T'CATG_A
Cuts at: 3752 3752
Size: 4576
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1047 1624 1838 2140 2521 1047
Size: 577 214 302 381 3102
Fragments arranged by size:
3102 577 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2866 2920 4406 4571 50
Size: 2816 54 1486 165 55
Fragments arranged by size:
2816 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 444 551 1077 1139 2486 444
Size: 107 526 62 1347 2534
Fragments arranged by size:
2534 1347 526 107 62
BsrFI r'CCGG_y
Cuts at: 2572 2753 2964 2572
Size: 181 211 4184
Fragments arranged by size:
4184 211 181
BsrGI T'GTAC_A
Cuts at: 428 428
Size: 4576
BssHII G'CGCG_C
Cuts at: 816 816
Size: 4576
BssSI C'ACGA_G
Cuts at: 2845 3205 2845
Size: 360 4216
BstAPI GCAn_nnn'nTGC
Cuts at: 1951 1951
Size: 4576
BstBI TT'CG_AA
Cuts at: 110 110
Size: 4576
BstYI r'GATC_y
Cuts at: 1 378 461 589 1599 2066 2089 2424 2670
Size: 377 83 128 1010 467 23 335 246
Cuts at: 2670 3684 3770 3782 4128 1
Size: 1014 86 12 346 449
Fragments arranged by size:
1014 1010 467 449 377 346 335 246
128 86 83 23 12
BstZ17I GTA'TAC
Cuts at: 1563 1563
Size: 4576
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2524 2705 2868 2524
Size: 181 163 4232
Fragments arranged by size:
4232 181 163
BtsI GCAGTG_nn'
Cuts at: 562 1817 562
Size: 1255 3321
DraI TTT'AAA
Cuts at: 894 1475 1573 894
Size: 581 98 3897
Fragments arranged by size:
3897 581 98
DrdI GACnn_nn'nnGTC
Cuts at: 2280 3140 2280
Size: 860 3716
EaeI y'GGCC_r
Cuts at: 936 2159 2333 2724 2751 4098 936
Size: 1223 174 391 27 1347 1414
Fragments arranged by size:
1414 1347 1223 391 174 27
EagI C'GGCC_G
Cuts at: 2159 2159
Size: 4576
EarI CTCTTCn'nnn_
Cuts at: 866 2597 2807 866
Size: 1731 210 2635
Fragments arranged by size:
2635 1731 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2782 3094 3240 4386 2782
Size: 312 146 1146 2972
Fragments arranged by size:
2972 1146 312 146
EcoICRI GAG'CTC
Cuts at: 1586 1586
Size: 4576
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 700 1156 1368 1481 1490 2399 2831 3580 3848
Size: 456 212 113 9 909 432 749 268
Cuts at: 3848 700
Size: 1428
Fragments arranged by size:
1428 909 749 456 432 268 212 113
9
EcoO109I rG'GnC_Cy
Cuts at: 1093 1713 1093
Size: 620 3956
EcoRI G'AATT_C
Cuts at: 1262 1578 1262
Size: 316 4260
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1282 2593 1282
Size: 1311 3265
FspI TGC'GCA
Cuts at: 2355 2355
Size: 4576
HaeII r_GCGC'y
Cuts at: 2008 2256 3280 4259 2008
Size: 248 1024 979 2325
Fragments arranged by size:
2325 1024 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2416 2446 2448 2478 2416
Size: 30 2 30 4514
Fragments arranged by size:
4514 30 30 2
HincII GTy'rAC
Cuts at: 291 1613 291
Size: 1322 3254
HindIII A'AGCT_T
Cuts at: 233 741 233
Size: 508 4068
HpaI GTT'AAC
Cuts at: 291 291
Size: 4576
KasI G'GCGC_C
Cuts at: 2004 2252 2004
Size: 248 4328
KpnI G_GTAC'C
Cuts at: 1594 1594
Size: 4576
MfeI C'AATT_G
Cuts at: 1418 1418
Size: 4576
MluI A'CGCG_T
Cuts at: 4009 4009
Size: 4576
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 340 3247 3431 340
Size: 2907 184 1485
Fragments arranged by size:
2907 1485 184
MscI TGG'CCA
Cuts at: 938 2335 938
Size: 1397 3179
MslI CAynn'nnrTG
Cuts at: 2563 2690 2563
Size: 127 4449
MspA1I CmG'CkG
Cuts at: 159 1459 1685 1999 2359 3374 3619 159
Size: 1300 226 314 360 1015 245 1116
Fragments arranged by size:
1300 1116 1015 360 314 245 226
NaeI GCC'GGC
Cuts at: 2755 2755
Size: 4576
NarI GG'CG_CC
Cuts at: 2005 2253 2005
Size: 248 4328
NdeI CA'TA_TG
Cuts at: 734 734
Size: 4576
NgoMIV G'CCGG_C
Cuts at: 2753 2753
Size: 4576
NruI TCG'CGA
Cuts at: 2937 2937
Size: 4576
NspI r_CATG'y
Cuts at: 429 1627 2127 3036 429
Size: 1198 500 909 1969
Fragments arranged by size:
1969 1198 909 500
PciI A'CATG_T
Cuts at: 3032 3032
Size: 4576
PflMI CCAn_nnn'nTGG
Cuts at: 934 1225 934
Size: 291 4285
PmeI GTTT'AAAC
Cuts at: 1573 1573
Size: 4576
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3715 3747 3715
Size: 32 4544
PpuMI rG'GwC_Cy
Cuts at: 1093 1093
Size: 4576
PshAI GACnn'nnGTC
Cuts at: 829 829
Size: 4576
PsiI TTA'TAA
Cuts at: 1154 1154
Size: 4576
PspXI vC'TCGA_Gb
Cuts at: 752 752
Size: 4576
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1437 1469 1437
Size: 32 4544
PstI C_TGCA'G
Cuts at: 569 780 1128 1621 2306 569
Size: 211 348 493 685 2839
Fragments arranged by size:
2839 685 493 348 211
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4576
PvuII CAG'CTG
Cuts at: 159 1459 1999 2359 159
Size: 1300 540 360 2376
Fragments arranged by size:
2376 1300 540 360
RsrII CG'GwC_CG
Cuts at: 2769 2769
Size: 4576
SacI G_AGCT'C
Cuts at: 1588 1588
Size: 4576
SalI G'TCGA_C
Cuts at: 1611 1611
Size: 4576
SapI GCTCTTCn'nnn_
Cuts at: 2597 2807 2597
Size: 210 4366
SbfI CC_TGCA'GG
Cuts at: 1621 1621
Size: 4576
ScaI AGT'ACT
Cuts at: 4093 4093
Size: 4576
SfcI C'TryA_G
Cuts at: 33 366 482 565 776 1082 1124 1454 1617
Size: 333 116 83 211 306 42 330 163
Cuts at: 1617 2302 3297 3488 33
Size: 685 995 191 1121
Fragments arranged by size:
1121 995 685 333 330 306 211 191
163 116 83 42
SfoI GGC'GCC
Cuts at: 2006 2254 2006
Size: 248 4328
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4576
SmaI CCC'GGG
Cuts at: 1596 1596
Size: 4576
SmlI C'TyrA_G
Cuts at: 752 1183 1729 2644 3138 3400 752
Size: 431 546 915 494 262 1928
Fragments arranged by size:
1928 915 546 494 431 262
SnaBI TAC'GTA
Cuts at: 1566 1566
Size: 4576
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4576
SphI G_CATG'C
Cuts at: 1627 2127 1627
Size: 500 4076
SspI AAT'ATT
Cuts at: 1534 1534
Size: 4576
StyI C'CwwG_G
Cuts at: 939 1708 2062 939
Size: 769 354 3453
Fragments arranged by size:
3453 769 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 291 1365 2157 2290 2749 2960 291
Size: 1074 792 133 459 211 1907
Fragments arranged by size:
1907 1074 792 459 211 133
TatI w'GTAC_w
Cuts at: 428 1509 4091 428
Size: 1081 2582 913
Fragments arranged by size:
2582 1081 913
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 605 637 4093 4125 605
Size: 32 3456 32 1056
Fragments arranged by size:
3456 1056 32 32
Tth111I GACn'n_nGTC
Cuts at: 2371 2371
Size: 4576
XbaI T'CTAG_A
Cuts at: 1605 1605
Size: 4576
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2069 2069
Size: 4576
XhoI C'TCGA_G
Cuts at: 752 752
Size: 4576
XmaI C'CCGG_G
Cuts at: 1594 1594
Size: 4576
XmnI GAAnn'nnTTC
Cuts at: 578 1449 578
Size: 871 3705
ZraI GAC'GTC
Cuts at: 1039 1039
Size: 4576
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstYI BstZ17I BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NdeI NgoMIV NruI NspI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PspXI PsrI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SnaBI SpeI
SphI SspI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AleI AloI ApaI AscI AvrII
BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI Bpu10I
BsaXI BsiWI BsmBI BstEII BstXI Bsu36I BtgI ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI NcoI NheI
NotI NsiI PacI PasI PfoI PmlI PspOMI SacII
SanDI SexAI SfiI SgrAI SrfI StuI SwaI