Restriction map for
pF1KSDB0029
(Circular) (Six-base) MAPSORT of: pF1KSDB0029.seq Check: 9331 from: 1 to: 4849
pF1KSDB0029 4849 bp
With 182 enzymes: SgfI *
June 20, 2008 10:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 409 691 409
Size: 282 4567
AccI GT'mk_AC
Cuts at: 1559 1885 1559
Size: 326 4523
Acc65I G'GTAC_C
Cuts at: 1863 1863
Size: 4849
AclI AA'CG_TT
Cuts at: 4689 4689
Size: 4849
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1116 2672 3104 3853 4121 1116
Size: 1556 432 749 268 1844
Fragments arranged by size:
1844 1556 749 432 268
AflIII A'CryG_T
Cuts at: 2199 3305 4282 2199
Size: 1106 977 2766
Fragments arranged by size:
2766 1106 977
AgeI A'CCGG_T
Cuts at: 3237 3237
Size: 4849
AleI CACnn'nnGTG
Cuts at: 1756 1756
Size: 4849
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1498 1498
Size: 4849
AlwNI CAG_nnn'CTG
Cuts at: 1372 1678 2152 3721 1372
Size: 306 474 1569 2500
Fragments arranged by size:
2500 1569 474 306
ApaLI G'TGCA_C
Cuts at: 3619 3619
Size: 4849
ApoI r'AATT_y
Cuts at: 1851 4202 4458 1851
Size: 2351 256 2242
Fragments arranged by size:
2351 2242 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4849
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4849
AvaI C'yCGr_G
Cuts at: 1682 1867 1682
Size: 185 4664
BamHI G'GATC_C
Cuts at: 1362 1872 1362
Size: 510 4339
BanI G'GyrC_C
Cuts at: 424 450 1186 1663 1750 1797 1863 2277 2525
Size: 26 736 477 87 47 66 414 248
Cuts at: 2525 2560 424
Size: 35 2713
Fragments arranged by size:
2713 736 477 414 248 87 66 47
35 26
BanII G_rGCy'C
Cuts at: 668 959 1051 1452 1690 1861 2891 668
Size: 291 92 401 238 171 1030 2626
Fragments arranged by size:
2626 1030 401 291 238 171 92
BbeI G_GCGC'C
Cuts at: 1801 2281 2529 1801
Size: 480 248 4121
Fragments arranged by size:
4121 480 248
BbsI GAAGACnn'nnnn_
Cuts at: 298 298
Size: 4849
BbvCI CC'TCA_GC
Cuts at: 824 824
Size: 4849
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1773 1807 1773
Size: 34 4815
BciVI GTATCCnnnnn_n'
Cuts at: 1319 2745 3508 4849 1319
Size: 1426 763 1341 1319
Fragments arranged by size:
1426 1341 1319 763
BclI T'GATC_A
Cuts at: 207 434 2367 207
Size: 227 1933 2689
Fragments arranged by size:
2689 1933 227
BglI GCCn_nnn'nGGC
Cuts at: 788 1018 1748 788
Size: 230 730 3889
Fragments arranged by size:
3889 730 230
BglII A'GATC_T
Cuts at: 674 1295 2362 4401 674
Size: 621 1067 2039 1122
Fragments arranged by size:
2039 1122 1067 621
BlpI GC'TnA_GC
Cuts at: 1776 1959 1776
Size: 183 4666
Bme1580I G_kGCm'C
Cuts at: 1122 1189 2472 2565 3623 1122
Size: 67 1283 93 1058 2348
Fragments arranged by size:
2348 1283 1058 93 67
BmrI ACTGGGnnnn_n'
Cuts at: 1017 2059 2140 2475 4621 1017
Size: 1042 81 335 2146 1245
Fragments arranged by size:
2146 1245 1042 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3214 3214
Size: 4849
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 302 347 302
Size: 45 4804
Bpu10I CC'TnA_GC
Cuts at: 414 455 824 1045 414
Size: 41 369 221 4218
Fragments arranged by size:
4218 369 221 41
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 695 2023 2902 3396 3694 695
Size: 1328 879 494 298 1850
Fragments arranged by size:
1850 1328 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 4553 4553
Size: 4849
BsaAI yAC'GTr
Cuts at: 1506 1839 2200 1506
Size: 333 361 4155
Fragments arranged by size:
4155 361 333
BsaBI GATnn'nnATC
Cuts at: 6 319 644 2361 4400 6
Size: 313 325 1717 2039 455
Fragments arranged by size:
2039 1717 455 325 313
BsaHI Gr'CG_yC
Cuts at: 1798 2278 2526 3230 4726 1798
Size: 480 248 704 1496 1921
Fragments arranged by size:
1921 1496 704 480 248
BsaWI w'CCGG_w
Cuts at: 1065 2036 2557 3237 3511 3658 4069 1065
Size: 971 521 680 274 147 411 1845
Fragments arranged by size:
1845 971 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1063 1093 1195 1225 1063
Size: 30 102 30 4687
Fragments arranged by size:
4687 102 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 299 1216 1442 299
Size: 917 226 3706
Fragments arranged by size:
3706 917 226
BseYI C'CCAG_C
Cuts at: 696 2272 3030 3609 4105 696
Size: 1576 758 579 496 1440
Fragments arranged by size:
1576 1440 758 579 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 601 923 1110 1486 601
Size: 322 187 376 3964
Fragments arranged by size:
3964 376 322 187
BsiEI CG_ry'CG
Cuts at: 104 1702 1812 2389 2435 3645 104
Size: 1598 110 577 46 1210 1308
Fragments arranged by size:
1598 1308 1210 577 110 46
BsiHKAI G_wGCw'C
Cuts at: 668 1861 2639 2829 3623 668
Size: 1193 778 190 794 1894
Fragments arranged by size:
1894 1193 794 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 178 1389 178
Size: 1211 3638
Bsp1286I G_dGCh'C
Cuts at: 668 959 1051 1122 1189 1452 1690 1861 2472
Size: 291 92 71 67 263 238 171 611
Cuts at: 2472 2565 2639 2829 2891 3623 668
Size: 93 74 190 62 732 1894
Fragments arranged by size:
1894 732 611 291 263 238 190 171
93 92 74 71 67 62
BspEI T'CCGG_A
Cuts at: 1065 4069 1065
Size: 3004 1845
BspHI T'CATG_A
Cuts at: 890 4025 890
Size: 3135 1714
BspMI ACCTGCnnnn'nnnn_
Cuts at: 409 691 1508 1604 1897 2111 2413 2794 409
Size: 282 817 96 293 214 302 381 2464
Fragments arranged by size:
2464 817 381 302 293 282 214 96
BsrBI CCG'CTC
Cuts at: 50 1206 3139 3193 4679 4844 50
Size: 1156 1933 54 1486 165 55
Fragments arranged by size:
1933 1486 1156 165 55 54
BsrDI GCAATG_nn'
Cuts at: 704 737 848 2759 704
Size: 33 111 1911 2794
Fragments arranged by size:
2794 1911 111 33
BsrFI r'CCGG_y
Cuts at: 2845 3026 3237 2845
Size: 181 211 4457
Fragments arranged by size:
4457 211 181
BssSI C'ACGA_G
Cuts at: 162 3118 3478 162
Size: 2956 360 1533
Fragments arranged by size:
2956 1533 360
BstAPI GCAn_nnn'nTGC
Cuts at: 579 1678 2224 579
Size: 1099 546 3204
Fragments arranged by size:
3204 1099 546
BstBI TT'CG_AA
Cuts at: 110 110
Size: 4849
BstXI CCAn_nnnn'nTGG
Cuts at: 1197 1519 1197
Size: 322 4527
BstYI r'GATC_y
Cuts at: 1 674 1098 1295 1362 1872 2339 2362 2697
Size: 673 424 197 67 510 467 23 335
Cuts at: 2697 2943 3957 4043 4055 4401 1
Size: 246 1014 86 12 346 449
Fragments arranged by size:
1014 673 510 467 449 424 346 335
246 197 86 67 23 12
Bsu36I CC'TnA_GG
Cuts at: 1181 1181
Size: 4849
BtgI C'CryG_G
Cuts at: 154 154
Size: 4849
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2797 2978 3141 2797
Size: 181 163 4505
Fragments arranged by size:
4505 181 163
BtsI GCAGTG_nn'
Cuts at: 962 1122 1136 2090 962
Size: 160 14 954 3721
Fragments arranged by size:
3721 954 160 14
ClaI AT'CG_AT
Cuts at: 335 335
Size: 4849
DraI TTT'AAA
Cuts at: 1846 1846
Size: 4849
DrdI GACnn_nn'nnGTC
Cuts at: 2553 3413 2553
Size: 860 3989
EaeI y'GGCC_r
Cuts at: 1088 1235 1521 1699 1809 2432 2606 2997 3024
Size: 147 286 178 110 623 174 391 27
Cuts at: 3024 4371 1088
Size: 1347 1566
Fragments arranged by size:
1566 1347 623 391 286 178 174 147
110 27
EagI C'GGCC_G
Cuts at: 1699 1809 2432 1699
Size: 110 623 4116
Fragments arranged by size:
4116 623 110
EarI CTCTTCn'nnn_
Cuts at: 508 806 2870 3080 508
Size: 298 2064 210 2277
Fragments arranged by size:
2277 2064 298 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3055 3367 3513 4659 3055
Size: 312 146 1146 3245
Fragments arranged by size:
3245 1146 312 146
EcoICRI GAG'CTC
Cuts at: 666 1859 666
Size: 1193 3656
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 302 347 1116 2672 3104 3853 4121 302
Size: 45 769 1556 432 749 268 1030
Fragments arranged by size:
1556 1030 769 749 432 268 45
EcoNI CCTnn'n_nnAGG
Cuts at: 181 475 592 181
Size: 294 117 4438
Fragments arranged by size:
4438 294 117
EcoO109I rG'GnC_Cy
Cuts at: 1986 1986
Size: 4849
EcoRI G'AATT_C
Cuts at: 1851 1851
Size: 4849
EcoRV GAT'ATC
Cuts at: 735 735
Size: 4849
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 429 1182 2866 429
Size: 753 1684 2412
Fragments arranged by size:
2412 1684 753
FspI TGC'GCA
Cuts at: 2628 2628
Size: 4849
HaeII r_GCGC'y
Cuts at: 1801 2281 2529 3553 4532 1801
Size: 480 248 1024 979 2118
Fragments arranged by size:
2118 1024 979 480 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 156 179 188 196 211 228 448 480 901
Size: 23 9 8 15 17 220 32 421
Cuts at: 901 933 1195 1227 2689 2719 2721 2751 156
Size: 32 262 32 1462 30 2 30 2254
Fragments arranged by size:
2254 1462 421 262 220 32 32 32
30 30 23 17 15 9 8 2
HincII GTy'rAC
Cuts at: 609 1886 609
Size: 1277 3572
HpaI GTT'AAC
Cuts at: 609 609
Size: 4849
KasI G'GCGC_C
Cuts at: 1797 2277 2525 1797
Size: 480 248 4121
Fragments arranged by size:
4121 480 248
KpnI G_GTAC'C
Cuts at: 1867 1867
Size: 4849
MfeI C'AATT_G
Cuts at: 864 1151 864
Size: 287 4562
MluI A'CGCG_T
Cuts at: 4282 4282
Size: 4849
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 743 1574 3520 3704 743
Size: 831 1946 184 1888
Fragments arranged by size:
1946 1888 831 184
MscI TGG'CCA
Cuts at: 1090 1237 2608 1090
Size: 147 1371 3331
Fragments arranged by size:
3331 1371 147
MslI CAynn'nnrTG
Cuts at: 430 557 1756 2836 2963 430
Size: 127 1199 1080 127 2316
Fragments arranged by size:
2316 1199 1080 127 127
MspA1I CmG'CkG
Cuts at: 156 907 1094 1958 2272 2632 3647 3892 156
Size: 751 187 864 314 360 1015 245 1113
Fragments arranged by size:
1113 1015 864 751 360 314 245 187
NaeI GCC'GGC
Cuts at: 3028 3028
Size: 4849
NarI GG'CG_CC
Cuts at: 1798 2278 2526 1798
Size: 480 248 4121
Fragments arranged by size:
4121 480 248
NgoMIV G'CCGG_C
Cuts at: 3026 3026
Size: 4849
NruI TCG'CGA
Cuts at: 3210 3210
Size: 4849
NspI r_CATG'y
Cuts at: 541 762 1603 1620 1900 2400 3309 541
Size: 221 841 17 280 500 909 2081
Fragments arranged by size:
2081 909 841 500 280 221 17
PasI CC'CwG_GG
Cuts at: 1512 1512
Size: 4849
PciI A'CATG_T
Cuts at: 3305 3305
Size: 4849
PflMI CCAn_nnn'nTGG
Cuts at: 1266 1266
Size: 4849
PfoI T'CCnGG_A
Cuts at: 279 279
Size: 4849
PmeI GTTT'AAAC
Cuts at: 1846 1846
Size: 4849
PmlI CAC'GTG
Cuts at: 1506 1506
Size: 4849
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 817 849 3988 4020 817
Size: 32 3139 32 1646
Fragments arranged by size:
3139 1646 32 32
PsiI TTA'TAA
Cuts at: 635 635
Size: 4849
PstI C_TGCA'G
Cuts at: 1106 1540 1894 2579 1106
Size: 434 354 685 3376
Fragments arranged by size:
3376 685 434 354
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4849
PvuII CAG'CTG
Cuts at: 907 2272 2632 907
Size: 1365 360 3124
Fragments arranged by size:
3124 1365 360
RsrII CG'GwC_CG
Cuts at: 3042 3042
Size: 4849
SacI G_AGCT'C
Cuts at: 668 1861 668
Size: 1193 3656
SacII CC_GC'GG
Cuts at: 157 157
Size: 4849
SalI G'TCGA_C
Cuts at: 1884 1884
Size: 4849
SapI GCTCTTCn'nnn_
Cuts at: 806 2870 3080 806
Size: 2064 210 2575
Fragments arranged by size:
2575 2064 210
SbfI CC_TGCA'GG
Cuts at: 1894 1894
Size: 4849
ScaI AGT'ACT
Cuts at: 4366 4366
Size: 4849
SfcI C'TryA_G
Cuts at: 33 1102 1536 1890 2575 3570 3761 33
Size: 1069 434 354 685 995 191 1121
Fragments arranged by size:
1121 1069 995 685 434 354 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 788 788
Size: 4849
SfoI GGC'GCC
Cuts at: 1799 2279 2527 1799
Size: 480 248 4121
Fragments arranged by size:
4121 480 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4849
SmaI CCC'GGG
Cuts at: 1869 1869
Size: 4849
SmlI C'TyrA_G
Cuts at: 710 2002 2917 3411 3673 710
Size: 1292 915 494 262 1886
Fragments arranged by size:
1886 1292 915 494 262
SnaBI TAC'GTA
Cuts at: 1839 1839
Size: 4849
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4849
SphI G_CATG'C
Cuts at: 1603 1900 2400 1603
Size: 297 500 4052
Fragments arranged by size:
4052 500 297
StuI AGG'CCT
Cuts at: 481 481
Size: 4849
StyI C'CwwG_G
Cuts at: 135 396 1013 1609 1981 2335 135
Size: 261 617 596 372 354 2649
Fragments arranged by size:
2649 617 596 372 354 261
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 565 1768 2430 2563 3022 3233 565
Size: 1203 662 133 459 211 2181
Fragments arranged by size:
2181 1203 662 459 211 133
TatI w'GTAC_w
Cuts at: 795 1415 4364 795
Size: 620 2949 1280
Fragments arranged by size:
2949 1280 620
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 150 182 1161 1193 4366 4398 150
Size: 32 979 32 3173 32 601
Fragments arranged by size:
3173 979 601 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 565 1351 1555 2644 565
Size: 786 204 1089 2770
Fragments arranged by size:
2770 1089 786 204
XbaI T'CTAG_A
Cuts at: 1878 1878
Size: 4849
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 312 1150 1245 2342 312
Size: 838 95 1097 2819
Fragments arranged by size:
2819 1097 838 95
XmaI C'CCGG_G
Cuts at: 1867 1867
Size: 4849
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NruI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PsiI PstI PvuI PvuII
RsrII SacI SacII SalI SapI SbfI ScaI SfcI
SfiI SfoI SgfI SmaI SmlI SnaBI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AloI ApaI AscI AvrII
BaeI BfrBI BmgBI BmtI BsiWI BsmI BsrGI BssHII
BstEII BstZ17I CspCI DraIII FseI FspAI HindIII NcoI
NdeI NheI NotI NsiI PacI PpuMI PshAI PspOMI
PspXI PsrI SanDI SexAI SgrAI SrfI SspI SwaI
XhoI XmnI ZraI