Restriction map for
pF1KSDB0028
(Circular) (Six-base) MAPSORT of: pF1KSDB0028.seq Check: 8509 from: 1 to: 4804
pF1KSDB0028 4804 bp
With 182 enzymes: SgfI *
June 20, 2008 10:36 ..
AccI GT'mk_AC
Cuts at: 1840 1840
Size: 4804
Acc65I G'GTAC_C
Cuts at: 1818 1818
Size: 4804
AclI AA'CG_TT
Cuts at: 1366 4644 1366
Size: 3278 1526
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 421 2627 3059 3808 4076 421
Size: 2206 432 749 268 1149
Fragments arranged by size:
2206 1149 749 432 268
AflIII A'CryG_T
Cuts at: 878 2154 3260 4237 878
Size: 1276 1106 977 1445
Fragments arranged by size:
1445 1276 1106 977
AgeI A'CCGG_T
Cuts at: 3192 3192
Size: 4804
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 464 464
Size: 4804
AlwNI CAG_nnn'CTG
Cuts at: 2107 3676 2107
Size: 1569 3235
ApaI G_GGCC'C
Cuts at: 435 435
Size: 4804
ApaLI G'TGCA_C
Cuts at: 3574 3574
Size: 4804
ApoI r'AATT_y
Cuts at: 1527 1806 4157 4413 1527
Size: 279 2351 256 1918
Fragments arranged by size:
2351 1918 279 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4804
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4804
AvaI C'yCGr_G
Cuts at: 290 1822 290
Size: 1532 3272
BamHI G'GATC_C
Cuts at: 1827 1827
Size: 4804
BanI G'GyrC_C
Cuts at: 1096 1818 2232 2480 2515 1096
Size: 722 414 248 35 3385
Fragments arranged by size:
3385 722 414 248 35
BanII G_rGCy'C
Cuts at: 435 1134 1597 1745 1757 1816 2846 435
Size: 699 463 148 12 59 1030 2393
Fragments arranged by size:
2393 1030 699 463 148 59 12
BbeI G_GCGC'C
Cuts at: 2236 2484 2236
Size: 248 4556
BbsI GAAGACnn'nnnn_
Cuts at: 430 430
Size: 4804
BciVI GTATCCnnnnn_n'
Cuts at: 1482 2700 3463 4804 1482
Size: 1218 763 1341 1482
Fragments arranged by size:
1482 1341 1218 763
BclI T'GATC_A
Cuts at: 2322 2322
Size: 4804
BglI GCCn_nnn'nGGC
Cuts at: 562 562
Size: 4804
BglII A'GATC_T
Cuts at: 591 2317 4356 591
Size: 1726 2039 1039
Fragments arranged by size:
2039 1726 1039
BlpI GC'TnA_GC
Cuts at: 1133 1914 1133
Size: 781 4023
Bme1580I G_kGCm'C
Cuts at: 435 1101 1406 2427 2520 3578 435
Size: 666 305 1021 93 1058 1661
Fragments arranged by size:
1661 1058 1021 666 305 93
BmrI ACTGGGnnnn_n'
Cuts at: 1515 1739 1751 2014 2095 2430 4576 1515
Size: 224 12 263 81 335 2146 1743
Fragments arranged by size:
2146 1743 335 263 224 81 12
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1470 3169 1470
Size: 1699 3105
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 165 908 1440 165
Size: 743 532 3529
Fragments arranged by size:
3529 743 532
Bpu10I CC'TnA_GC
Cuts at: 1115 1115
Size: 4804
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 313 606 1978 2857 3351 3649 313
Size: 293 1372 879 494 298 1468
Fragments arranged by size:
1468 1372 879 494 298 293
BsaI GGTCTCn'nnnn_
Cuts at: 1165 4508 1165
Size: 3343 1461
BsaAI yAC'GTr
Cuts at: 1794 2155 1794
Size: 361 4443
BsaBI GATnn'nnATC
Cuts at: 6 2316 4355 6
Size: 2310 2039 455
Fragments arranged by size:
2310 2039 455
BsaHI Gr'CG_yC
Cuts at: 2233 2481 3185 4681 2233
Size: 248 704 1496 2356
Fragments arranged by size:
2356 1496 704 248
BsaWI w'CCGG_w
Cuts at: 245 1991 2512 3192 3466 3613 4024 245
Size: 1746 521 680 274 147 411 1025
Fragments arranged by size:
1746 1025 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1411 1441 1411
Size: 30 4774
BseYI C'CCAG_C
Cuts at: 1100 1388 1578 1672 2227 2985 3564 4060 1100
Size: 288 190 94 555 758 579 496 1844
Fragments arranged by size:
1844 758 579 555 496 288 190 94
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 992 992
Size: 4804
BsiEI CG_ry'CG
Cuts at: 104 2344 2390 3600 104
Size: 2240 46 1210 1308
Fragments arranged by size:
2240 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 1597 1816 2594 2784 3578 1597
Size: 219 778 190 794 2823
Fragments arranged by size:
2823 794 778 219 190
Bsp1286I G_dGCh'C
Cuts at: 435 1101 1134 1406 1597 1745 1757 1816 2427
Size: 666 33 272 191 148 12 59 611
Cuts at: 2427 2520 2594 2784 2846 3578 435
Size: 93 74 190 62 732 1661
Fragments arranged by size:
1661 732 666 611 272 191 190 148
93 74 62 59 33 12
BspEI T'CCGG_A
Cuts at: 4024 4024
Size: 4804
BspHI T'CATG_A
Cuts at: 456 3980 456
Size: 3524 1280
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1852 2066 2368 2749 1852
Size: 214 302 381 3907
Fragments arranged by size:
3907 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3094 3148 4634 4799 50
Size: 3044 54 1486 165 55
Fragments arranged by size:
3044 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 470 1037 2714 470
Size: 567 1677 2560
Fragments arranged by size:
2560 1677 567
BsrFI r'CCGG_y
Cuts at: 1783 2800 2981 3192 1783
Size: 1017 181 211 3395
Fragments arranged by size:
3395 1017 211 181
BssSI C'ACGA_G
Cuts at: 745 1302 3073 3433 745
Size: 557 1771 360 2116
Fragments arranged by size:
2116 1771 557 360
BstAPI GCAn_nnn'nTGC
Cuts at: 1621 2179 1621
Size: 558 4246
BstBI TT'CG_AA
Cuts at: 110 110
Size: 4804
BstYI r'GATC_y
Cuts at: 1 591 708 1827 2294 2317 2652 2898 3912
Size: 590 117 1119 467 23 335 246 1014
Cuts at: 3912 3998 4010 4356 1
Size: 86 12 346 449
Fragments arranged by size:
1119 1014 590 467 449 346 335 246
117 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1017 1017
Size: 4804
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1125 2752 2933 3096 1125
Size: 1627 181 163 2833
Fragments arranged by size:
2833 1627 181 163
BtsI GCAGTG_nn'
Cuts at: 210 667 1001 2045 210
Size: 457 334 1044 2969
Fragments arranged by size:
2969 1044 457 334
DraI TTT'AAA
Cuts at: 231 1801 231
Size: 1570 3234
DrdI GACnn_nn'nnGTC
Cuts at: 2508 3368 2508
Size: 860 3944
EaeI y'GGCC_r
Cuts at: 542 1186 1196 2387 2561 2952 2979 4326 542
Size: 644 10 1191 174 391 27 1347 1020
Fragments arranged by size:
1347 1191 1020 644 391 174 27 10
EagI C'GGCC_G
Cuts at: 2387 2387
Size: 4804
EarI CTCTTCn'nnn_
Cuts at: 689 1649 1679 2825 3035 689
Size: 960 30 1146 210 2458
Fragments arranged by size:
2458 1146 960 210 30
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1199 1385 3010 3322 3468 4614 1199
Size: 186 1625 312 146 1146 1389
Fragments arranged by size:
1625 1389 1146 312 186 146
EcoICRI GAG'CTC
Cuts at: 1595 1814 1595
Size: 219 4585
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 165 421 908 1440 2627 3059 3808 4076 165
Size: 256 487 532 1187 432 749 268 893
Fragments arranged by size:
1187 893 749 532 487 432 268 256
EcoO109I rG'GnC_Cy
Cuts at: 431 432 1270 1941 431
Size: 1 838 671 3294
Fragments arranged by size:
3294 838 671 1
EcoRI G'AATT_C
Cuts at: 1806 1806
Size: 4804
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2821 2821
Size: 4804
FspI TGC'GCA
Cuts at: 2583 2583
Size: 4804
HaeII r_GCGC'y
Cuts at: 170 350 1040 2236 2484 3508 4487 170
Size: 180 690 1196 248 1024 979 487
Fragments arranged by size:
1196 1024 979 690 487 248 180
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 886 918 1411 1443 2644 2674 2676 2706 886
Size: 32 493 32 1201 30 2 30 2984
Fragments arranged by size:
2984 1201 493 32 32 30 30 2
HincII GTy'rAC
Cuts at: 131 838 1841 131
Size: 707 1003 3094
Fragments arranged by size:
3094 1003 707
KasI G'GCGC_C
Cuts at: 2232 2480 2232
Size: 248 4556
KpnI G_GTAC'C
Cuts at: 1822 1822
Size: 4804
MluI A'CGCG_T
Cuts at: 4237 4237
Size: 4804
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1101 1597 1745 3475 3659 1101
Size: 496 148 1730 184 2246
Fragments arranged by size:
2246 1730 496 184 148
MscI TGG'CCA
Cuts at: 544 1188 2563 544
Size: 644 1375 2785
Fragments arranged by size:
2785 1375 644
MslI CAynn'nnrTG
Cuts at: 446 1625 2791 2918 446
Size: 1179 1166 127 2332
Fragments arranged by size:
2332 1179 1166 127
MspA1I CmG'CkG
Cuts at: 1255 1913 2227 2587 3602 3847 1255
Size: 658 314 360 1015 245 2212
Fragments arranged by size:
2212 1015 658 360 314 245
NaeI GCC'GGC
Cuts at: 2983 2983
Size: 4804
NarI GG'CG_CC
Cuts at: 2233 2481 2233
Size: 248 4556
NgoMIV G'CCGG_C
Cuts at: 2981 2981
Size: 4804
NruI TCG'CGA
Cuts at: 3165 3165
Size: 4804
NspI r_CATG'y
Cuts at: 882 1855 2355 3264 882
Size: 973 500 909 2422
Fragments arranged by size:
2422 973 909 500
PciI A'CATG_T
Cuts at: 878 3260 878
Size: 2382 2422
PflMI CCAn_nnn'nTGG
Cuts at: 1145 1145
Size: 4804
PmeI GTTT'AAAC
Cuts at: 1801 1801
Size: 4804
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3943 3975 3943
Size: 32 4772
PpuMI rG'GwC_Cy
Cuts at: 1270 1270
Size: 4804
PspOMI G'GGCC_C
Cuts at: 431 431
Size: 4804
PstI C_TGCA'G
Cuts at: 1849 2534 1849
Size: 685 4119
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4804
PvuII CAG'CTG
Cuts at: 1255 2227 2587 1255
Size: 972 360 3472
Fragments arranged by size:
3472 972 360
RsrII CG'GwC_CG
Cuts at: 2997 2997
Size: 4804
SacI G_AGCT'C
Cuts at: 1597 1816 1597
Size: 219 4585
SalI G'TCGA_C
Cuts at: 1839 1839
Size: 4804
SapI GCTCTTCn'nnn_
Cuts at: 1679 2825 3035 1679
Size: 1146 210 3448
Fragments arranged by size:
3448 1146 210
SbfI CC_TGCA'GG
Cuts at: 1849 1849
Size: 4804
ScaI AGT'ACT
Cuts at: 4321 4321
Size: 4804
SexAI A'CCwGG_T
Cuts at: 1315 1315
Size: 4804
SfcI C'TryA_G
Cuts at: 33 1845 2530 3525 3716 33
Size: 1812 685 995 191 1121
Fragments arranged by size:
1812 1121 995 685 191
SfoI GGC'GCC
Cuts at: 2234 2482 2234
Size: 248 4556
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4804
SmaI CCC'GGG
Cuts at: 1824 1824
Size: 4804
SmlI C'TyrA_G
Cuts at: 328 585 1957 2872 3366 3628 328
Size: 257 1372 915 494 262 1504
Fragments arranged by size:
1504 1372 915 494 262 257
SnaBI TAC'GTA
Cuts at: 1794 1794
Size: 4804
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4804
SphI G_CATG'C
Cuts at: 1855 2355 1855
Size: 500 4304
SspI AAT'ATT
Cuts at: 689 689
Size: 4804
StuI AGG'CCT
Cuts at: 1450 1714 1450
Size: 264 4540
StyI C'CwwG_G
Cuts at: 1266 1936 2290 1266
Size: 670 354 3780
Fragments arranged by size:
3780 670 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1084 1348 2385 2518 2977 3188 1084
Size: 264 1037 133 459 211 2700
Fragments arranged by size:
2700 1037 459 264 211 133
TatI w'GTAC_w
Cuts at: 722 4319 722
Size: 3597 1207
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 646 678 4321 4353 646
Size: 32 3643 32 1097
Fragments arranged by size:
3643 1097 32 32
Tth111I GACn'n_nGTC
Cuts at: 2599 2599
Size: 4804
XbaI T'CTAG_A
Cuts at: 1833 1833
Size: 4804
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1350 2297 1350
Size: 947 3857
XmaI C'CCGG_G
Cuts at: 1822 1822
Size: 4804
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI Bsu36I BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NruI
NspI PciI PflMI PmeI PpiI PpuMI PspOMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI AscI
AvrII BaeI BbvCI BcgI BfrBI BmgBI BmtI BseRI
BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI BstZ17I
BtgI ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NcoI NdeI NheI NotI NsiI
PacI PasI PfoI PmlI PshAI PsiI PspXI PsrI
SacII SanDI SfiI SgrAI SrfI SwaI XhoI XmnI
ZraI